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The map label for this gene is pnp

Identifier: 54025857

GI number: 54025857

Start: 4099851

End: 4102145

Strand: Reverse

Name: pnp

Synonym: nfa38870

Alternate gene names: 54025857

Gene position: 4102145-4099851 (Counterclockwise)

Preceding gene: 54025858

Following gene: 54025856

Centisome position: 68.13

GC content: 69.98

Gene sequence:

>2295_bases
ATGACGCAGACAAGTGAACGCAAGTCCTCGGCCGTGGAGGTCGAGCCGGGTGTGTTCGAATCGGTCGCGCTGATCGACAA
CGGCAGCTACGGCACCCGCACGGTGCGGTTCGAGACCGGCAGGCTGGCGCGCCAGGCCGCCGGGTCGGTCGTGGCCTACC
TGGACGACGAGACCATGCTCCTGTCGGCCACCACCGCGGGCAAGACCCCGAAGGACCAGTTCGACTTCTTCCCGCTGACG
GTCGACGTCGAGGAGCGGATGTACGCCGCGGGCCGCATCCCCGGCTCGTTCTTCCGCCGCGAGGGCCGCCCCTCCACCGA
CGCGATCCTGACCTGCCGCCTGATCGACCGCCCGCTGCGCCCGTCGTTCGTCGACGGCCTGCGCAACGAGGTCCAGGTCG
TGGTCACCGTGCTGAGCCTGGATCCGAAGGATCTCTACGACGTGGTGGCCATCAACGCCGCCTCCGCGTCCACCCAGATC
GCGGGCCTGCCGTTCTCCGGCCCGGTCGGCGGCGTGCGCGTCGCCCTGATCCCGGACCAGGGTGCGAACTCCGCGGGCGG
TGGCCAGTGGGTGGCGTTCCCGACCGTGGAGCAGCTCGAGGGCGCCGTGTTCGACATGGTGGTCGCGGGCCGGGTCGTGG
AATCCGGTGACGTCGCGATCATGATGGTCGAGGCCGAGGCCACCGAAAAGGTCATCGAGCTGGTCGAGGGCGGCGCGCAG
GCGCCGACCGAGGCCGTGGTGGCCGAGGGACTCGAGGCGGCCAAGCCGTTCATCGCCCGGCTGTGCCGCGCCCAGCAGGA
CCTGGCCGAGCTGGCGGCCAAGCCGACCGAGGAGTTCCCGCTCTTCCCGCCCTACGGCCCCGACGTGTACGAGGCCGTCG
AAGGCGCGGCCGAGGCCGAGCTGGGCGAGGCGCTGAGCATCGCGGGCAAGCAGGAGCGCGAGGAGAAGATCGACGAGATC
AAGCTCGCCGTGCTCGACCGGCTGGCCGAGCAGTTCGCCGGCCGGGAGAAGGAGCTGGGCGCGGCGTTCCGCTCGGTCAC
CAAGAAGCTGGTCCGTCAGCGCATCCTCACCGACGGTTTCCGCATCGACGGCCGCGGTCTGGCCGACATCCGCGCGCTCT
CGGCCGAGGTGGCCGTGGTGCCGCGCGCGCACGGCTCGGCGCTGTTCGAGCGCGGTGAGACCCAGATCCTGGGCGTCACC
ACGCTGGACATGGTGAAGATGGCGCAGCAGGTCGACTCGCTCGGCCCGGAGACCAGCAAGCGCTACATGCACCACTACAA
CTTCCCGCCGTTCTCCACCGGTGAGACCGGCCGGGTCGGTTCGCCCAAGCGGCGCGAGATCGGCCACGGTGCGCTGGCCG
AGCGGGCGCTGATCCCGGTGCTGCCCAGCCAGGAGGATTTCCCGTACGCGATCCGTCAGGTCTCCGAGGCGCTGGGCTCC
AACGGCTCGACCTCGATGGGCTCGGTGTGTGCCTCCACCCTGTCGCTGCTCAACGCCGGTGTGCCGCTCAAGGCGCCGGT
CGCGGGCATCGCGATGGGCCTGGTGTCGGACACCGTCACCAACGACAAGGGTGAGCAGGAGGTCCGCTACGTCGCGCTGA
CCGACATCCTCGGCGCCGAGGACGCCTTCGGCGACATGGACTTCAAGGTCGCAGGCACCCGCGAGTTCGTCACCGCGCTG
CAGCTGGACACCAAGCTCGACGGCATCCCCTCGCAGGTGCTCGCCGGTGCGCTGAGCCAGGCGCACGACGCCCGCACCAC
CATCCTGGACGTGATGGCCGAGGCCATCGCCACCCCGGACGAGATGAGCCCCTACGCTCCGCGCGTCACCGCGATCAAGA
TCCCGGTCGACAAGATCGGCGAGGTGATCGGGCCCAAGGGCAAGGTCATCAACCAGATCACCGAGGACACCGGCGCCAAC
ATCTCCATCGAGGACGACGGCACCGTGTTCGTCGGCGCGACCGACGGCCCCTCGGCCCAGGCCGCGATCGACGCGATCAA
CGCCATCGCCAACCCGCAGCTGCCCAAGGTGGGCGAGCGCTTCCTCGGCACGGTCGTCAAGACCACCGCCTTCGGCGCGT
TCGTCTCGCTGCTGCCGGGTCGCGACGGTCTGGTGCACATCTCCAAGCTGGGCAACGGCAAGCGCGTCGCCAAGGTCGAG
GACGTGGTGAACGTCGGCGACAAGCTGCGCGTGGAGATCGCCGACATCGACAACCGGGGCAAGATCTCGCTGGTCCCGGT
CGACGAGAACGCCGACGAGCCCGCGGCGGATGCCGTCGACGCCGGAACCGAGTAG

Upstream 100 bases:

>100_bases
CAGGAGGGCTACCGCGCGACCGTGCCGGGTACGGCTGACGACAGCCGGATCGCGTCCGACGGACGCTCGATCCGGCGAGA
CGAGAGGTTGAGAGAAGACA

Downstream 100 bases:

>100_bases
TACTGGTCGGTGTGAGCAAGAAGAAGACGACGACCCCCCTGATCCCGAGCGGGCAGGGGGGTCCGTCACTACGGCAGCGG
GTCGACGAGGCGGTGGACTC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 764; Mature: 763

Protein sequence:

>764_residues
MTQTSERKSSAVEVEPGVFESVALIDNGSYGTRTVRFETGRLARQAAGSVVAYLDDETMLLSATTAGKTPKDQFDFFPLT
VDVEERMYAAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLRNEVQVVVTVLSLDPKDLYDVVAINAASASTQI
AGLPFSGPVGGVRVALIPDQGANSAGGGQWVAFPTVEQLEGAVFDMVVAGRVVESGDVAIMMVEAEATEKVIELVEGGAQ
APTEAVVAEGLEAAKPFIARLCRAQQDLAELAAKPTEEFPLFPPYGPDVYEAVEGAAEAELGEALSIAGKQEREEKIDEI
KLAVLDRLAEQFAGREKELGAAFRSVTKKLVRQRILTDGFRIDGRGLADIRALSAEVAVVPRAHGSALFERGETQILGVT
TLDMVKMAQQVDSLGPETSKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALIPVLPSQEDFPYAIRQVSEALGS
NGSTSMGSVCASTLSLLNAGVPLKAPVAGIAMGLVSDTVTNDKGEQEVRYVALTDILGAEDAFGDMDFKVAGTREFVTAL
QLDTKLDGIPSQVLAGALSQAHDARTTILDVMAEAIATPDEMSPYAPRVTAIKIPVDKIGEVIGPKGKVINQITEDTGAN
ISIEDDGTVFVGATDGPSAQAAIDAINAIANPQLPKVGERFLGTVVKTTAFGAFVSLLPGRDGLVHISKLGNGKRVAKVE
DVVNVGDKLRVEIADIDNRGKISLVPVDENADEPAADAVDAGTE

Sequences:

>Translated_764_residues
MTQTSERKSSAVEVEPGVFESVALIDNGSYGTRTVRFETGRLARQAAGSVVAYLDDETMLLSATTAGKTPKDQFDFFPLT
VDVEERMYAAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLRNEVQVVVTVLSLDPKDLYDVVAINAASASTQI
AGLPFSGPVGGVRVALIPDQGANSAGGGQWVAFPTVEQLEGAVFDMVVAGRVVESGDVAIMMVEAEATEKVIELVEGGAQ
APTEAVVAEGLEAAKPFIARLCRAQQDLAELAAKPTEEFPLFPPYGPDVYEAVEGAAEAELGEALSIAGKQEREEKIDEI
KLAVLDRLAEQFAGREKELGAAFRSVTKKLVRQRILTDGFRIDGRGLADIRALSAEVAVVPRAHGSALFERGETQILGVT
TLDMVKMAQQVDSLGPETSKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALIPVLPSQEDFPYAIRQVSEALGS
NGSTSMGSVCASTLSLLNAGVPLKAPVAGIAMGLVSDTVTNDKGEQEVRYVALTDILGAEDAFGDMDFKVAGTREFVTAL
QLDTKLDGIPSQVLAGALSQAHDARTTILDVMAEAIATPDEMSPYAPRVTAIKIPVDKIGEVIGPKGKVINQITEDTGAN
ISIEDDGTVFVGATDGPSAQAAIDAINAIANPQLPKVGERFLGTVVKTTAFGAFVSLLPGRDGLVHISKLGNGKRVAKVE
DVVNVGDKLRVEIADIDNRGKISLVPVDENADEPAADAVDAGTE
>Mature_763_residues
TQTSERKSSAVEVEPGVFESVALIDNGSYGTRTVRFETGRLARQAAGSVVAYLDDETMLLSATTAGKTPKDQFDFFPLTV
DVEERMYAAGRIPGSFFRREGRPSTDAILTCRLIDRPLRPSFVDGLRNEVQVVVTVLSLDPKDLYDVVAINAASASTQIA
GLPFSGPVGGVRVALIPDQGANSAGGGQWVAFPTVEQLEGAVFDMVVAGRVVESGDVAIMMVEAEATEKVIELVEGGAQA
PTEAVVAEGLEAAKPFIARLCRAQQDLAELAAKPTEEFPLFPPYGPDVYEAVEGAAEAELGEALSIAGKQEREEKIDEIK
LAVLDRLAEQFAGREKELGAAFRSVTKKLVRQRILTDGFRIDGRGLADIRALSAEVAVVPRAHGSALFERGETQILGVTT
LDMVKMAQQVDSLGPETSKRYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALIPVLPSQEDFPYAIRQVSEALGSN
GSTSMGSVCASTLSLLNAGVPLKAPVAGIAMGLVSDTVTNDKGEQEVRYVALTDILGAEDAFGDMDFKVAGTREFVTALQ
LDTKLDGIPSQVLAGALSQAHDARTTILDVMAEAIATPDEMSPYAPRVTAIKIPVDKIGEVIGPKGKVINQITEDTGANI
SIEDDGTVFVGATDGPSAQAAIDAINAIANPQLPKVGERFLGTVVKTTAFGAFVSLLPGRDGLVHISKLGNGKRVAKVED
VVNVGDKLRVEIADIDNRGKISLVPVDENADEPAADAVDAGTE

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=696, Percent_Identity=35.4885057471264, Blast_Score=373, Evalue=1e-103,
Organism=Escherichia coli, GI145693187, Length=720, Percent_Identity=45.6944444444444, Blast_Score=559, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI115534063, Length=726, Percent_Identity=32.7823691460055, Blast_Score=309, Evalue=3e-84,
Organism=Drosophila melanogaster, GI281362905, Length=721, Percent_Identity=34.9514563106796, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24651641, Length=721, Percent_Identity=34.9514563106796, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24651643, Length=721, Percent_Identity=34.9514563106796, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI161079377, Length=693, Percent_Identity=33.9105339105339, Blast_Score=358, Evalue=5e-99,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_NOCFA (Q5YSV6)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_120099.1
- HSSP:   P05055
- ProteinModelPortal:   Q5YSV6
- SMR:   Q5YSV6
- GeneID:   3111377
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa38870
- NMPDR:   fig|247156.1.peg.3783
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- BioCyc:   NFAR247156:NFA38870-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR014069
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591
- TIGRFAMs:   TIGR02696

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 80876; Mature: 80745

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTSERKSSAVEVEPGVFESVALIDNGSYGTRTVRFETGRLARQAAGSVVAYLDDETML
CCCCCCCCCCCEEECCCCCCEEEEEECCCCCCEEEEEECHHHHHHHCCCEEEEECCCEEE
LSATTAGKTPKDQFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSTDAILTCRLIDRPLR
EEECCCCCCCCCCCCEEEEEEEHHHHHHHHCCCCHHHHHCCCCCCHHHEEEEHHHCCCCC
PSFVDGLRNEVQVVVTVLSLDPKDLYDVVAINAASASTQIAGLPFSGPVGGVRVALIPDQ
CHHHHHHHHHHEEEEEEECCCHHHHHHHHEEECCCCCCEEECCCCCCCCCCEEEEEECCC
GANSAGGGQWVAFPTVEQLEGAVFDMVVAGRVVESGDVAIMMVEAEATEKVIELVEGGAQ
CCCCCCCCCEEECCCHHHHHHHHHHHHHHCCEEECCCEEEEEEECHHHHHHHHHHHCCCC
APTEAVVAEGLEAAKPFIARLCRAQQDLAELAAKPTEEFPLFPPYGPDVYEAVEGAAEAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCHHHHH
LGEALSIAGKQEREEKIDEIKLAVLDRLAEQFAGREKELGAAFRSVTKKLVRQRILTDGF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
RIDGRGLADIRALSAEVAVVPRAHGSALFERGETQILGVTTLDMVKMAQQVDSLGPETSK
EECCCCHHHHHHHCCCEEEEECCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCHHHH
RYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALIPVLPSQEDFPYAIRQVSEALGS
HHHHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHHCCEECCCCCCCCCCHHHHHHHHHHCC
NGSTSMGSVCASTLSLLNAGVPLKAPVAGIAMGLVSDTVTNDKGEQEVRYVALTDILGAE
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHEEEEEEHHHHCCC
DAFGDMDFKVAGTREFVTALQLDTKLDGIPSQVLAGALSQAHDARTTILDVMAEAIATPD
CCCCCCCEEEECHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EMSPYAPRVTAIKIPVDKIGEVIGPKGKVINQITEDTGANISIEDDGTVFVGATDGPSAQ
CCCCCCCCEEEEECCHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCCEEEEECCCCCCHH
AAIDAINAIANPQLPKVGERFLGTVVKTTAFGAFVSLLPGRDGLVHISKLGNGKRVAKVE
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHH
DVVNVGDKLRVEIADIDNRGKISLVPVDENADEPAADAVDAGTE
HHHCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHCCCCC
>Mature Secondary Structure 
TQTSERKSSAVEVEPGVFESVALIDNGSYGTRTVRFETGRLARQAAGSVVAYLDDETML
CCCCCCCCCCEEECCCCCCEEEEEECCCCCCEEEEEECHHHHHHHCCCEEEEECCCEEE
LSATTAGKTPKDQFDFFPLTVDVEERMYAAGRIPGSFFRREGRPSTDAILTCRLIDRPLR
EEECCCCCCCCCCCCEEEEEEEHHHHHHHHCCCCHHHHHCCCCCCHHHEEEEHHHCCCCC
PSFVDGLRNEVQVVVTVLSLDPKDLYDVVAINAASASTQIAGLPFSGPVGGVRVALIPDQ
CHHHHHHHHHHEEEEEEECCCHHHHHHHHEEECCCCCCEEECCCCCCCCCCEEEEEECCC
GANSAGGGQWVAFPTVEQLEGAVFDMVVAGRVVESGDVAIMMVEAEATEKVIELVEGGAQ
CCCCCCCCCEEECCCHHHHHHHHHHHHHHCCEEECCCEEEEEEECHHHHHHHHHHHCCCC
APTEAVVAEGLEAAKPFIARLCRAQQDLAELAAKPTEEFPLFPPYGPDVYEAVEGAAEAE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHCCHHHHH
LGEALSIAGKQEREEKIDEIKLAVLDRLAEQFAGREKELGAAFRSVTKKLVRQRILTDGF
HHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
RIDGRGLADIRALSAEVAVVPRAHGSALFERGETQILGVTTLDMVKMAQQVDSLGPETSK
EECCCCHHHHHHHCCCEEEEECCCCHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCHHHH
RYMHHYNFPPFSTGETGRVGSPKRREIGHGALAERALIPVLPSQEDFPYAIRQVSEALGS
HHHHHCCCCCCCCCCCCCCCCCHHHHCCCCHHHHCCEECCCCCCCCCCHHHHHHHHHHCC
NGSTSMGSVCASTLSLLNAGVPLKAPVAGIAMGLVSDTVTNDKGEQEVRYVALTDILGAE
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCHHEEEEEEHHHHCCC
DAFGDMDFKVAGTREFVTALQLDTKLDGIPSQVLAGALSQAHDARTTILDVMAEAIATPD
CCCCCCCEEEECHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
EMSPYAPRVTAIKIPVDKIGEVIGPKGKVINQITEDTGANISIEDDGTVFVGATDGPSAQ
CCCCCCCCEEEEECCHHHHHHHHCCCCHHHHHHHHCCCCEEEECCCCEEEEECCCCCCHH
AAIDAINAIANPQLPKVGERFLGTVVKTTAFGAFVSLLPGRDGLVHISKLGNGKRVAKVE
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHH
DVVNVGDKLRVEIADIDNRGKISLVPVDENADEPAADAVDAGTE
HHHCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA