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The map label for this gene is dut

Identifier: 54025723

GI number: 54025723

Start: 3971937

End: 3972434

Strand: Reverse

Name: dut

Synonym: nfa37530

Alternate gene names: 54025723

Gene position: 3972434-3971937 (Counterclockwise)

Preceding gene: 54025725

Following gene: 54025722

Centisome position: 65.97

GC content: 73.69

Gene sequence:

>498_bases
GTGAGCGCACTTTCACCGATACCGCTGCGGCGGCTGGATCCCGGCATCCCCGTGCCGACGCGCGCCCATCCCGGCGACGC
GGGCGTCGACCTGTGCACCACCGAGGACGTCGTGCTGGCGCCGGGGGAGCGGGTGCTGGTCGGCACCGGCATCGCGGTGG
CGCTGCCGGTGGGCACCGTCGGCCTGATCCATCCCCGCTCCGGCCTGGCCGCCAAGACCGGCCTGTCGGTGGTGAACACC
CCCGGCACCGTGGACGCGGGCTACCGGGGCGAGATCAAGGTGTGCCTGATCAATCACGACCCGCGTACGCCGATCGAGCT
GCGCCGTGGCGACCGCATCGCGCAGCTGCTCGTGCAGCGGGTGGAACTGGTGGACTTCGTCGAGGTGGAGCAGCTCGACG
AGACCTCCCGCGGCGCGGGTGGCCACGGGTCCAGCGGCGGTCATGCGAGCTTGACCCCGGGCGCGCGGAGCGCGGCCCGG
GTGGCCCAGGAGGGGTGA

Upstream 100 bases:

>100_bases
CGACGCTCGGTCACCGACGGGTTGGGCTGGTCCGACACGTTTCCACAGTAAGTCACCGTCACGGCGGCGCGCCGTCCAGG
TACGCGTAGGCTCGACCACC

Downstream 100 bases:

>100_bases
GACGGGAATGTTCGGCAGGAAGAAGCGCGACACCAAGAGATTCGAGTCCGACGCCTACGACGAGGACTACGCGGATCAGG
GGTACGACGAGGCTTACGAC

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 165; Mature: 164

Protein sequence:

>165_residues
MSALSPIPLRRLDPGIPVPTRAHPGDAGVDLCTTEDVVLAPGERVLVGTGIAVALPVGTVGLIHPRSGLAAKTGLSVVNT
PGTVDAGYRGEIKVCLINHDPRTPIELRRGDRIAQLLVQRVELVDFVEVEQLDETSRGAGGHGSSGGHASLTPGARSAAR
VAQEG

Sequences:

>Translated_165_residues
MSALSPIPLRRLDPGIPVPTRAHPGDAGVDLCTTEDVVLAPGERVLVGTGIAVALPVGTVGLIHPRSGLAAKTGLSVVNT
PGTVDAGYRGEIKVCLINHDPRTPIELRRGDRIAQLLVQRVELVDFVEVEQLDETSRGAGGHGSSGGHASLTPGARSAAR
VAQEG
>Mature_164_residues
SALSPIPLRRLDPGIPVPTRAHPGDAGVDLCTTEDVVLAPGERVLVGTGIAVALPVGTVGLIHPRSGLAAKTGLSVVNTP
GTVDAGYRGEIKVCLINHDPRTPIELRRGDRIAQLLVQRVELVDFVEVEQLDETSRGAGGHGSSGGHASLTPGARSAARV
AQEG

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=138, Percent_Identity=42.0289855072464, Blast_Score=104, Evalue=3e-23,
Organism=Homo sapiens, GI4503423, Length=140, Percent_Identity=41.4285714285714, Blast_Score=104, Evalue=3e-23,
Organism=Homo sapiens, GI70906441, Length=138, Percent_Identity=42.0289855072464, Blast_Score=103, Evalue=6e-23,
Organism=Escherichia coli, GI1790071, Length=150, Percent_Identity=36.6666666666667, Blast_Score=87, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI71988561, Length=145, Percent_Identity=41.3793103448276, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319729, Length=130, Percent_Identity=42.3076923076923, Blast_Score=101, Evalue=6e-23,
Organism=Drosophila melanogaster, GI19921126, Length=128, Percent_Identity=40.625, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24583610, Length=128, Percent_Identity=40.625, Blast_Score=91, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_NOCFA (Q5YT90)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_119965.1
- ProteinModelPortal:   Q5YT90
- SMR:   Q5YT90
- GeneID:   3109764
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa37530
- NMPDR:   fig|247156.1.peg.3655
- HOGENOM:   HBG436079
- OMA:   GTIDEGY
- ProtClustDB:   PRK00601
- BioCyc:   NFAR247156:NFA37530-MONOMER
- BRENDA:   3.6.1.23
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 16997; Mature: 16865

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: BINDING 79-79 BINDING 93-93

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALSPIPLRRLDPGIPVPTRAHPGDAGVDLCTTEDVVLAPGERVLVGTGIAVALPVGTV
CCCCCCCCHHHCCCCCCCCCCCCCCCCCEEEECCCCEEECCCCEEEEECCEEEEECCCEE
GLIHPRSGLAAKTGLSVVNTPGTVDAGYRGEIKVCLINHDPRTPIELRRGDRIAQLLVQR
EEEECCCCCCHHCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCEECCCCHHHHHHHHH
VELVDFVEVEQLDETSRGAGGHGSSGGHASLTPGARSAARVAQEG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCHHHHHHHCCC
>Mature Secondary Structure 
SALSPIPLRRLDPGIPVPTRAHPGDAGVDLCTTEDVVLAPGERVLVGTGIAVALPVGTV
CCCCCCCHHHCCCCCCCCCCCCCCCCCEEEECCCCEEECCCCEEEEECCEEEEECCCEE
GLIHPRSGLAAKTGLSVVNTPGTVDAGYRGEIKVCLINHDPRTPIELRRGDRIAQLLVQR
EEEECCCCCCHHCCCEEEECCCCCCCCCCCCEEEEEECCCCCCCCEECCCCHHHHHHHHH
VELVDFVEVEQLDETSRGAGGHGSSGGHASLTPGARSAARVAQEG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA