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The map label for this gene is fmt

Identifier: 54025578

GI number: 54025578

Start: 3818897

End: 3819820

Strand: Reverse

Name: fmt

Synonym: nfa36080

Alternate gene names: 54025578

Gene position: 3819820-3818897 (Counterclockwise)

Preceding gene: 54025579

Following gene: 54025577

Centisome position: 63.44

GC content: 72.51

Gene sequence:

>924_bases
ATGCGCCTGGTCTTCGCGGGCACGCCCGAACCCGCCGTCCCGTCGCTGCGCCGCCTCATCGCCTCGCCGCGGCACGAGGT
GGTCGCCGTGGTCACCCGGCCCGACGCGGTCGCCGGTCGCGGCCGCAAGATCACCCGATCGCCGATCGCGGCACTGGCCG
ACGAGCACGGCATCCCGGTGCTCTCGCCGCGCACACCCGCCGAACCCGAATTCCTGGACCGGTTGACCGAGCTGGCGCCG
GACTGCTGCCCGGTCGTCGCCTACGGTGCCTTGCTGCCCCAGGCCGCCCTGGACATTCCGCGGCACGGCTGGATCAACCT
GCACTTCTCCCTGCTGCCCGCCTGGCGCGGCGCGGCACCCGTGCAGGCCGCCATCAACGCCGGTGAGGAGATCACCGGCG
CCACCACCTTCCAGATCGAGGCCGGCCTCGACAGCGGCCCGGTCTACGGCGTCGTCACCGAGAAGATCGATGTCACCGAC
ACCGCGGGGACGTTGCTCGAACGACTGGCCGAAACCGGCGCCCGGCTGTTGGAGACCACGCTCGACGGCGTCGAGGACGG
CACGCTGCAGGCGGTGCCGCAGCCGACCGAAGGCGTGTCCTACGCGCCGAAGGTCACCCCCGAGGACGGACGGATCCGCT
GGGATCAGCCCGCGCTCGCGGTGAACCGGCGCATCCGCGCCGTCACTCCCGCGCCTGGAGCCTGGACGCTCGTCGGCGGT
AACCGGCTCAAGCTCGGCCCGGTGGAGCTGGTCGAGGAGCAGCTGCCCGAGCGGGTGATCGAGGTGCGCAAGTCCGGCGT
GTTCGTCGGTACCGCCACCACCGCCGTCCGCTTGGACAAGGTGCAGCCCCAGGGCAAGAAGATGATGCCCGCCCTCGACT
GGGCCCGCGGCGCCCGCCTACAGCCCGGCGCGGTGGTCGAGTGA

Upstream 100 bases:

>100_bases
CGATGCGCGCCATCCGCGAGTCCGACTGGTTCGAGGCGGGCATCACCGTGCGCGAGAGTCAGGGCGTCGGCCAGTCCGGT
CCGTTCGGACGGACCCGCTG

Downstream 100 bases:

>100_bases
GCGCCGCCGAGCGTCCGAGAAAGGCCGACGACCGCGACATCGACGGCGGGTGGGCCCGTCGCGCCGCCCGCGAGCAGGAA
GCCCGCCGGTCCGGGCGCAA

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MRLVFAGTPEPAVPSLRRLIASPRHEVVAVVTRPDAVAGRGRKITRSPIAALADEHGIPVLSPRTPAEPEFLDRLTELAP
DCCPVVAYGALLPQAALDIPRHGWINLHFSLLPAWRGAAPVQAAINAGEEITGATTFQIEAGLDSGPVYGVVTEKIDVTD
TAGTLLERLAETGARLLETTLDGVEDGTLQAVPQPTEGVSYAPKVTPEDGRIRWDQPALAVNRRIRAVTPAPGAWTLVGG
NRLKLGPVELVEEQLPERVIEVRKSGVFVGTATTAVRLDKVQPQGKKMMPALDWARGARLQPGAVVE

Sequences:

>Translated_307_residues
MRLVFAGTPEPAVPSLRRLIASPRHEVVAVVTRPDAVAGRGRKITRSPIAALADEHGIPVLSPRTPAEPEFLDRLTELAP
DCCPVVAYGALLPQAALDIPRHGWINLHFSLLPAWRGAAPVQAAINAGEEITGATTFQIEAGLDSGPVYGVVTEKIDVTD
TAGTLLERLAETGARLLETTLDGVEDGTLQAVPQPTEGVSYAPKVTPEDGRIRWDQPALAVNRRIRAVTPAPGAWTLVGG
NRLKLGPVELVEEQLPERVIEVRKSGVFVGTATTAVRLDKVQPQGKKMMPALDWARGARLQPGAVVE
>Mature_307_residues
MRLVFAGTPEPAVPSLRRLIASPRHEVVAVVTRPDAVAGRGRKITRSPIAALADEHGIPVLSPRTPAEPEFLDRLTELAP
DCCPVVAYGALLPQAALDIPRHGWINLHFSLLPAWRGAAPVQAAINAGEEITGATTFQIEAGLDSGPVYGVVTEKIDVTD
TAGTLLERLAETGARLLETTLDGVEDGTLQAVPQPTEGVSYAPKVTPEDGRIRWDQPALAVNRRIRAVTPAPGAWTLVGG
NRLKLGPVELVEEQLPERVIEVRKSGVFVGTATTAVRLDKVQPQGKKMMPALDWARGARLQPGAVVE

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family

Homologues:

Organism=Homo sapiens, GI238814322, Length=290, Percent_Identity=26.8965517241379, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI21614513, Length=294, Percent_Identity=28.2312925170068, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI164663775, Length=253, Percent_Identity=28.8537549407115, Blast_Score=83, Evalue=3e-16,
Organism=Escherichia coli, GI1789683, Length=296, Percent_Identity=41.2162162162162, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1788589, Length=285, Percent_Identity=28.7719298245614, Blast_Score=113, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI133930964, Length=282, Percent_Identity=26.9503546099291, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI28571984, Length=267, Percent_Identity=31.0861423220974, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI45550868, Length=249, Percent_Identity=31.7269076305221, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI24585660, Length=326, Percent_Identity=24.8466257668712, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): FMT_NOCFA (Q5YTN5)

Other databases:

- EMBL:   AP006618
- RefSeq:   YP_119820.1
- ProteinModelPortal:   Q5YTN5
- SMR:   Q5YTN5
- GeneID:   3109675
- GenomeReviews:   AP006618_GR
- KEGG:   nfa:nfa36080
- NMPDR:   fig|247156.1.peg.3521
- HOGENOM:   HBG571560
- OMA:   IMQMDEG
- ProtClustDB:   PRK00005
- BioCyc:   NFAR247156:NFA36080-MONOMER
- BRENDA:   2.1.2.9
- HAMAP:   MF_00182
- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR015518
- Gene3D:   G3DSA:3.10.25.10
- Gene3D:   G3DSA:3.40.50.170
- PANTHER:   PTHR11138
- TIGRFAMs:   TIGR00460

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N; SSF50486 FMT_C_like; SSF53328 formyl_transf

EC number: =2.1.2.9

Molecular weight: Translated: 32745; Mature: 32745

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLVFAGTPEPAVPSLRRLIASPRHEVVAVVTRPDAVAGRGRKITRSPIAALADEHGIPV
CEEEECCCCCCCHHHHHHHHCCCCCEEEEEEECCCHHCCCCCCCCCCHHHHHHHCCCCCE
LSPRTPAEPEFLDRLTELAPDCCPVVAYGALLPQAALDIPRHGWINLHFSLLPAWRGAAP
ECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCC
VQAAINAGEEITGATTFQIEAGLDSGPVYGVVTEKIDVTDTAGTLLERLAETGARLLETT
HHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEECEEECCCHHHHHHHHHHHHHHHHHHHH
LDGVEDGTLQAVPQPTEGVSYAPKVTPEDGRIRWDQPALAVNRRIRAVTPAPGAWTLVGG
HCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEECCCHHHHCCEEEEECCCCCCEEEECC
NRLKLGPVELVEEQLPERVIEVRKSGVFVGTATTAVRLDKVQPQGKKMMPALDWARGARL
CEEEECHHHHHHHHHHHHHHHHHHCCEEEEECHHEEEEECCCCCCCCCCCHHHHHCCCCC
QPGAVVE
CCCCCCC
>Mature Secondary Structure
MRLVFAGTPEPAVPSLRRLIASPRHEVVAVVTRPDAVAGRGRKITRSPIAALADEHGIPV
CEEEECCCCCCCHHHHHHHHCCCCCEEEEEEECCCHHCCCCCCCCCCHHHHHHHCCCCCE
LSPRTPAEPEFLDRLTELAPDCCPVVAYGALLPQAALDIPRHGWINLHFSLLPAWRGAAP
ECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCCCC
VQAAINAGEEITGATTFQIEAGLDSGPVYGVVTEKIDVTDTAGTLLERLAETGARLLETT
HHHHHCCCCCCCCCEEEEEECCCCCCCEEEEEECEEECCCHHHHHHHHHHHHHHHHHHHH
LDGVEDGTLQAVPQPTEGVSYAPKVTPEDGRIRWDQPALAVNRRIRAVTPAPGAWTLVGG
HCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEECCCHHHHCCEEEEECCCCCCEEEECC
NRLKLGPVELVEEQLPERVIEVRKSGVFVGTATTAVRLDKVQPQGKKMMPALDWARGARL
CEEEECHHHHHHHHHHHHHHHHHHCCEEEEECHHEEEEECCCCCCCCCCCHHHHHCCCCC
QPGAVVE
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA