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The map label for this gene is phnE [H]

Identifier: 54025498

GI number: 54025498

Start: 3740081

End: 3741679

Strand: Reverse

Name: phnE [H]

Synonym: nfa35280

Alternate gene names: 54025498

Gene position: 3741679-3740081 (Counterclockwise)

Preceding gene: 54025499

Following gene: 54025497

Centisome position: 62.14

GC content: 71.79

Gene sequence:

>1599_bases
GTGACCGCGACGCTCACCACACCGGGGCCCGTCCTGCCCGGCTCGCCGCCGCGGGCCGAGCGGGTGCGGGCGGCGCTGCT
GCGGCTGCTCGGGGTGGGCGGTCTCGTGCTGACCCTGATCTCGGCCTGGTCGATCGATTTCGCCCCGGCCACCCTGCTGG
ACGGTCTCGACGACATGGCCGCGCTGCTCGAGCGGATGCTGCCGCCGCGACTGGACGATCCGCCGCGGATCTGGGCGCTC
GCGGTGGAGACGCTGCTGATGGCGGTGCTTGGCACCGTGCTGGCGGCACTGGCCTCGATGCCGTTGGCGTTCCTGGCGGC
ACGCAACACCACCCCGCACCCGGCGGTGGCGGCGATCGCGCGCGGGATCATCACCTTCTGCCGGGCCATGCCGGACCTGT
TGTTCGCGGCGTTGCTGGTGCGCGCGCTCGGCATCGGTGTGCTGCCCGGCATTCTCGCGCTGGCGCTGCACTCCATCGGC
ATGCTCGGCAAGCTGTTCGCCGACGCGATCGAGCAGACCGATCCCGGCCCGCGCGAGGCCGCCCGGGCGGCGGGCGCCGG
GTACGTGCGCGAGATGATCCACGCCGTGGTGCCGCAGACGGTCCCGTCCTGGATCGGCACGTTCGTCTATCGCGTCGACA
TCAACCTGCGGATCTCGGTGGTGCTCGGCTTCGTCGGCGCGGGCGGCATCGGTTTCGCGCTGCAGGATTCGTTGCGCGGG
CTGGCCTATCCCCGCGCGCTGGGCATCGTGGCGGTGATCCTGGCGATCATCGCGGCGATGGAGGTGTTCGCGATCCTCGC
GCGGCGGGTGCTGCTGAGTCCGGAGTCCCTCGGACCCCGGGCCGCCAGGGCGGCCCGCACGGCCTTCGGCGCGATCGCCG
TGGCGGCCACGGTGGCCGCGCTGGTGGTGCTCGACGTCGATCCGGTGGAGCTGCTGACCTGGCCTACCGCCGCCGTCGAG
GTGTTCGGGCGGCTGCTGCCGCCGGATTTCGCCGCGCTGGGCCCGCAGTTGCTCGAGGCGGCCTGGCAGACGGTCGCGAT
CGCCGTGGTGGCGACCGCGATCGGGGCGGTACTGTCAGTGCCCGTCGGAATCCTCGCCGCCCGCACGGTGACTCCGCATC
CGGCGGTGTACTGGGCTGCGCGGGCCTGGATCCTGGTCGTGCGCGCGATCCCCGAGCTGATCCTGGCCGTGATCTTCGTC
GCCGCACTGGGCCTCGGGCCGATCGCGGGTACCTGCGCGCTGGCCATCGGCTCGGTCGGGCTGCTCGGCAAACTCGTCGC
CGACGCCGTCGAGGAGATCGACACGGGCCCGCTCGAAGCGGTCCGTGCGCTCGGCGGCGGCTGGTGGAAGACGTTGGTGG
CGGCGGTGATTCCGCAGGCGGTGCCCGCGCTGGTTGGTTCGACGCTCTACCTGCTCGACGTGAACATCCGCACCTCCACG
ATCCTGGGGATCGTGGGCGCGGGCGGGATCGGTTACCTGTTGTTCGAGTCGATCCGCACGCTCGAGTTCGGCGTCGCCGG
CGCGATCGTGCTCGTCGTCTTCGCGCTCGTCTACTTCATCGAAAGGCTCTCCGGATGGATTCGCTCCCAGGTGGTGTGA

Upstream 100 bases:

>100_bases
ACCGGCCCGCCGCCGGACTGTCCCGCGACGAGGTCATGGCGATCTACCAGCGGGCCGAGGTCACCCGGTCGGCGCCCGCC
GGACTGGCCGCGGGGGCGCT

Downstream 100 bases:

>100_bases
CGCGCGCGGCGGTGTGGACCGGTGCCGGGGTCGCGGTCCGCGACGTGCCGGTGCCGCGCCTGGCGCCGGGCCAATCGCTG
GTGCGGGTCCGCCTCGCCAC

Product: putative transporter

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 532; Mature: 531

Protein sequence:

>532_residues
MTATLTTPGPVLPGSPPRAERVRAALLRLLGVGGLVLTLISAWSIDFAPATLLDGLDDMAALLERMLPPRLDDPPRIWAL
AVETLLMAVLGTVLAALASMPLAFLAARNTTPHPAVAAIARGIITFCRAMPDLLFAALLVRALGIGVLPGILALALHSIG
MLGKLFADAIEQTDPGPREAARAAGAGYVREMIHAVVPQTVPSWIGTFVYRVDINLRISVVLGFVGAGGIGFALQDSLRG
LAYPRALGIVAVILAIIAAMEVFAILARRVLLSPESLGPRAARAARTAFGAIAVAATVAALVVLDVDPVELLTWPTAAVE
VFGRLLPPDFAALGPQLLEAAWQTVAIAVVATAIGAVLSVPVGILAARTVTPHPAVYWAARAWILVVRAIPELILAVIFV
AALGLGPIAGTCALAIGSVGLLGKLVADAVEEIDTGPLEAVRALGGGWWKTLVAAVIPQAVPALVGSTLYLLDVNIRTST
ILGIVGAGGIGYLLFESIRTLEFGVAGAIVLVVFALVYFIERLSGWIRSQVV

Sequences:

>Translated_532_residues
MTATLTTPGPVLPGSPPRAERVRAALLRLLGVGGLVLTLISAWSIDFAPATLLDGLDDMAALLERMLPPRLDDPPRIWAL
AVETLLMAVLGTVLAALASMPLAFLAARNTTPHPAVAAIARGIITFCRAMPDLLFAALLVRALGIGVLPGILALALHSIG
MLGKLFADAIEQTDPGPREAARAAGAGYVREMIHAVVPQTVPSWIGTFVYRVDINLRISVVLGFVGAGGIGFALQDSLRG
LAYPRALGIVAVILAIIAAMEVFAILARRVLLSPESLGPRAARAARTAFGAIAVAATVAALVVLDVDPVELLTWPTAAVE
VFGRLLPPDFAALGPQLLEAAWQTVAIAVVATAIGAVLSVPVGILAARTVTPHPAVYWAARAWILVVRAIPELILAVIFV
AALGLGPIAGTCALAIGSVGLLGKLVADAVEEIDTGPLEAVRALGGGWWKTLVAAVIPQAVPALVGSTLYLLDVNIRTST
ILGIVGAGGIGYLLFESIRTLEFGVAGAIVLVVFALVYFIERLSGWIRSQVV
>Mature_531_residues
TATLTTPGPVLPGSPPRAERVRAALLRLLGVGGLVLTLISAWSIDFAPATLLDGLDDMAALLERMLPPRLDDPPRIWALA
VETLLMAVLGTVLAALASMPLAFLAARNTTPHPAVAAIARGIITFCRAMPDLLFAALLVRALGIGVLPGILALALHSIGM
LGKLFADAIEQTDPGPREAARAAGAGYVREMIHAVVPQTVPSWIGTFVYRVDINLRISVVLGFVGAGGIGFALQDSLRGL
AYPRALGIVAVILAIIAAMEVFAILARRVLLSPESLGPRAARAARTAFGAIAVAATVAALVVLDVDPVELLTWPTAAVEV
FGRLLPPDFAALGPQLLEAAWQTVAIAVVATAIGAVLSVPVGILAARTVTPHPAVYWAARAWILVVRAIPELILAVIFVA
ALGLGPIAGTCALAIGSVGLLGKLVADAVEEIDTGPLEAVRALGGGWWKTLVAAVIPQAVPALVGSTLYLLDVNIRTSTI
LGIVGAGGIGYLLFESIRTLEFGVAGAIVLVVFALVYFIERLSGWIRSQVV

Specific function: Part of the ABC transporter complex phnCDE involved in phosphate import. Responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ABC transmembrane type-1 domains [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 55107; Mature: 54976

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATLTTPGPVLPGSPPRAERVRAALLRLLGVGGLVLTLISAWSIDFAPATLLDGLDDMA
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHCHHHHH
ALLERMLPPRLDDPPRIWALAVETLLMAVLGTVLAALASMPLAFLAARNTTPHPAVAAIA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
RGIITFCRAMPDLLFAALLVRALGIGVLPGILALALHSIGMLGKLFADAIEQTDPGPREA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
ARAAGAGYVREMIHAVVPQTVPSWIGTFVYRVDINLRISVVLGFVGAGGIGFALQDSLRG
HHHCCHHHHHHHHHHHHCHHHHHHHHHHHHEEECCEEHHHHHHHHCCCCCHHHHHHHHHH
LAYPRALGIVAVILAIIAAMEVFAILARRVLLSPESLGPRAARAARTAFGAIAVAATVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LVVLDVDPVELLTWPTAAVEVFGRLLPPDFAALGPQLLEAAWQTVAIAVVATAIGAVLSV
HHHHCCCHHHHHHCCHHHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
PVGILAARTVTPHPAVYWAARAWILVVRAIPELILAVIFVAALGLGPIAGTCALAIGSVG
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LLGKLVADAVEEIDTGPLEAVRALGGGWWKTLVAAVIPQAVPALVGSTLYLLDVNIRTST
HHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHH
ILGIVGAGGIGYLLFESIRTLEFGVAGAIVLVVFALVYFIERLSGWIRSQVV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TATLTTPGPVLPGSPPRAERVRAALLRLLGVGGLVLTLISAWSIDFAPATLLDGLDDMA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHCHHHHH
ALLERMLPPRLDDPPRIWALAVETLLMAVLGTVLAALASMPLAFLAARNTTPHPAVAAIA
HHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
RGIITFCRAMPDLLFAALLVRALGIGVLPGILALALHSIGMLGKLFADAIEQTDPGPREA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
ARAAGAGYVREMIHAVVPQTVPSWIGTFVYRVDINLRISVVLGFVGAGGIGFALQDSLRG
HHHCCHHHHHHHHHHHHCHHHHHHHHHHHHEEECCEEHHHHHHHHCCCCCHHHHHHHHHH
LAYPRALGIVAVILAIIAAMEVFAILARRVLLSPESLGPRAARAARTAFGAIAVAATVAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LVVLDVDPVELLTWPTAAVEVFGRLLPPDFAALGPQLLEAAWQTVAIAVVATAIGAVLSV
HHHHCCCHHHHHHCCHHHHHHHHHHCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHH
PVGILAARTVTPHPAVYWAARAWILVVRAIPELILAVIFVAALGLGPIAGTCALAIGSVG
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LLGKLVADAVEEIDTGPLEAVRALGGGWWKTLVAAVIPQAVPALVGSTLYLLDVNIRTST
HHHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCHHHH
ILGIVGAGGIGYLLFESIRTLEFGVAGAIVLVVFALVYFIERLSGWIRSQVV
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA