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The map label for this gene is ksgA

Identifier: 54020652

GI number: 54020652

Start: 847071

End: 847850

Strand: Reverse

Name: ksgA

Synonym: mhp673

Alternate gene names: 54020652

Gene position: 847850-847071 (Counterclockwise)

Preceding gene: 54020653

Following gene: 54020651

Centisome position: 94.97

GC content: 27.44

Gene sequence:

>780_bases
ATGCAAAAACCGGTTCCTAAGAAGCGTTTAGGTCAGAATTTTTTAAAAGATAGAAAAATTGCTGAAAAAATAGTTGAAAA
TATTGATCTTAAAAACAAAGAAATTATCGAAATTGGCTGCGGAACCGGTTTTTTGACAAATTTTTTGCTTGAAAAAGCAA
AATTTGTTACTTGTTATGAAATTGATAAAAATTTAATTCCAATTCTTGAAAAAAAATTCAAGAATAAAAATCTTCGTATA
ATTAACGAAGATTTTTTGCTCGCAGAGTTTGAATCTAAAGAAAAAAAAACAATTATAGCCAACCTTCCTTACTATATTAC
CTCAAAAATTTTGTTTAAAATCTTTGCAAATTTTGAAAAATTTGATAAAATTATTTTAATGGTTCAAAACGAGGTCGCCG
ATCGAATAGTGGCCAAGCCAAAAACACCAACTTATTCAAAATTATCGCTTGCATCACAATATATAGCGAAGGTCAGAAAA
TTGTTTGTAGTTGGCCCGGATTCGTTTTTTCCAAAACCGAAAGTCAATTCAGCTGTTGTTTCTTTTGATTTTAGGGCAAA
CTTGGACGCCAAAGAAATGGAGAATTTTTTTTGGTTTACAAAAAGATGTTTTCAATTTAAACGAAAAACTCTATATAATA
ATCTTATTTTTTTTCTAAATAAGCAGCAAATTGAAAAAATTTATAATTTTTTTCAATTTGCTCAAAATATAAGACCTCAA
CAGCTTGATTTGGTAACTTATATAAGGTTAGCCGACTTTTATTTTAACAATATTTTTTAA

Upstream 100 bases:

>100_bases
CAGTGAACTTATCAAACGATAGCAAATTTAAAAAATTTGACAAAAATAGAACTTTCTGAAATAATTTATAGAAATGTAAG
GAAAATTTTTAAGGTAAAAA

Downstream 100 bases:

>100_bases
AAAAAATATTTTGTTTTTTTAAAAAATATTGTATAATTTTAATACATTCTTGACAACCAAAAATTTGGTTGCTTATTTAT
TATTTTTTCTTATTTTTTTG

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MQKPVPKKRLGQNFLKDRKIAEKIVENIDLKNKEIIEIGCGTGFLTNFLLEKAKFVTCYEIDKNLIPILEKKFKNKNLRI
INEDFLLAEFESKEKKTIIANLPYYITSKILFKIFANFEKFDKIILMVQNEVADRIVAKPKTPTYSKLSLASQYIAKVRK
LFVVGPDSFFPKPKVNSAVVSFDFRANLDAKEMENFFWFTKRCFQFKRKTLYNNLIFFLNKQQIEKIYNFFQFAQNIRPQ
QLDLVTYIRLADFYFNNIF

Sequences:

>Translated_259_residues
MQKPVPKKRLGQNFLKDRKIAEKIVENIDLKNKEIIEIGCGTGFLTNFLLEKAKFVTCYEIDKNLIPILEKKFKNKNLRI
INEDFLLAEFESKEKKTIIANLPYYITSKILFKIFANFEKFDKIILMVQNEVADRIVAKPKTPTYSKLSLASQYIAKVRK
LFVVGPDSFFPKPKVNSAVVSFDFRANLDAKEMENFFWFTKRCFQFKRKTLYNNLIFFLNKQQIEKIYNFFQFAQNIRPQ
QLDLVTYIRLADFYFNNIF
>Mature_259_residues
MQKPVPKKRLGQNFLKDRKIAEKIVENIDLKNKEIIEIGCGTGFLTNFLLEKAKFVTCYEIDKNLIPILEKKFKNKNLRI
INEDFLLAEFESKEKKTIIANLPYYITSKILFKIFANFEKFDKIILMVQNEVADRIVAKPKTPTYSKLSLASQYIAKVRK
LFVVGPDSFFPKPKVNSAVVSFDFRANLDAKEMENFFWFTKRCFQFKRKTLYNNLIFFLNKQQIEKIYNFFQFAQNIRPQ
QLDLVTYIRLADFYFNNIF

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI7657198, Length=233, Percent_Identity=33.4763948497854, Blast_Score=108, Evalue=4e-24,
Organism=Homo sapiens, GI156415992, Length=280, Percent_Identity=28.5714285714286, Blast_Score=101, Evalue=7e-22,
Organism=Escherichia coli, GI1786236, Length=265, Percent_Identity=28.6792452830189, Blast_Score=115, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI25146882, Length=207, Percent_Identity=35.7487922705314, Blast_Score=99, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25141369, Length=206, Percent_Identity=29.6116504854369, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324989, Length=196, Percent_Identity=33.1632653061224, Blast_Score=97, Evalue=3e-21,
Organism=Drosophila melanogaster, GI21358017, Length=234, Percent_Identity=33.3333333333333, Blast_Score=111, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_MYCH2 (Q5ZZN4)

Other databases:

- EMBL:   AE017332
- RefSeq:   YP_116181.1
- ProteinModelPortal:   Q5ZZN4
- SMR:   Q5ZZN4
- STRING:   Q5ZZN4
- GeneID:   3105664
- GenomeReviews:   AE017332_GR
- KEGG:   mhy:mhp673
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   LAVLMYQ
- PhylomeDB:   Q5ZZN4
- ProtClustDB:   PRK00274
- BioCyc:   MHYO295358:MHP673-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 30736; Mature: 30736

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 13-13 BINDING 15-15 BINDING 39-39 BINDING 60-60 BINDING 84-84 BINDING 101-101

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKPVPKKRLGQNFLKDRKIAEKIVENIDLKNKEIIEIGCGTGFLTNFLLEKAKFVTCYE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHEEEEEE
IDKNLIPILEKKFKNKNLRIINEDFLLAEFESKEKKTIIANLPYYITSKILFKIFANFEK
CCCCHHHHHHHHHCCCCCEEEECCEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHCHHH
FDKIILMVQNEVADRIVAKPKTPTYSKLSLASQYIAKVRKLFVVGPDSFFPKPKVNSAVV
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEE
SFDFRANLDAKEMENFFWFTKRCFQFKRKTLYNNLIFFLNKQQIEKIYNFFQFAQNIRPQ
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHCCCCH
QLDLVTYIRLADFYFNNIF
HHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQKPVPKKRLGQNFLKDRKIAEKIVENIDLKNKEIIEIGCGTGFLTNFLLEKAKFVTCYE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHEEEEEE
IDKNLIPILEKKFKNKNLRIINEDFLLAEFESKEKKTIIANLPYYITSKILFKIFANFEK
CCCCHHHHHHHHHCCCCCEEEECCEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHCHHH
FDKIILMVQNEVADRIVAKPKTPTYSKLSLASQYIAKVRKLFVVGPDSFFPKPKVNSAVV
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEE
SFDFRANLDAKEMENFFWFTKRCFQFKRKTLYNNLIFFLNKQQIEKIYNFFQFAQNIRPQ
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECHHHHHHHHHHHHHHHCCCCH
QLDLVTYIRLADFYFNNIF
HHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA