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The map label for this gene is leuS

Identifier: 54020648

GI number: 54020648

Start: 840470

End: 842737

Strand: Reverse

Name: leuS

Synonym: mhp667

Alternate gene names: 54020648

Gene position: 842737-840470 (Counterclockwise)

Preceding gene: 54020649

Following gene: 54020647

Centisome position: 94.4

GC content: 30.73

Gene sequence:

>2268_bases
ATGTTTCCCTATCCTTCGGCTTCTGGTCTGCATCTAGGTCATCCGATTGGCTATACGGCTTCTGATATTGTTGCTAGATT
TAAACGTTTAAATGGTTTTGACGTGCTTCATCCGATGGGTTGGGATGCTTTTGGTTTGCCAGCAGAGCAATATGCGATAA
AAACAGGTAATCATCCGGGTGAATTTACAAAAAGAAATATTGAAAATTTTAAAAAACAAATAATTTCTTTTGGATTTTCT
TATGATTGGGATAAGGAAATTAACACCTCTGATCCAGAATTTTATGTCCAAACTCAGTGGATTTTTAAACTATTATATAA
GGCAAATTTAGCTGAGATTCGCGAAGTTGAAGTCAATTGATGCCCGGAATTAGGCACTGTTTTAGCAAATGAGGAATTAA
AAAGGGAAAAAAATGGTGCGCTTGTCAGTGAAAGAGGAAATTTTCCTGTAATTTTAAAAAAAATGAAACAGTGAGTTTTG
AAAATCACTGAATATGCACAGAAATTACTTGATGGACTTGATCAAGTTGATTTTCCTGATTCTTTAAAGGCTTTACAAAA
AAAATGAATCGGTAAATCAAAAGGGTGAAAAATTAAATTTTTAATTGAAAAAACCGGTGAATTTCTTGAAATTTTTACAA
CAAAAATCGAAACTTTTTATGGAGTAAGTTTCCTAGCCCTTGCACCTTTACATAATTTTGCTCAAAAATTGGCTAAAAAT
AATGAAAAAATCAGCCAATTTATTGAGCAAAACAGCTTTTCAAGTCCAAAATTACAAAGTAAAATGACAGGAATTTTCAC
TGGATTATATGCTATTCATCCTTTAAATTCAAGTAAAATTCCGATTTATATTGCAAATTATGTTCTAAATAACTATGCAA
CTTCTGCAATTATGGGGGTTCCTGGCCATAATTTAGCTGATTTAGAATTTGCTCAAATTTTCCAAATTGACTATGTTAAA
GTAATAAATCAAGAAAATTTACTTATAAATTCTGCAGAATTTTCTGGTTTGACTATTAAAGACGGAGCAAATTCTATTTT
CAATAAGCTAAATTCAATGAATTTAGTTCAAAAAACTACAATTTTTCGACTAAAAGATTGAGTTTTTTCCCGTCAGAGAT
ACTGAGGAGAACCTTTTCCGGTTTATTTTGATGAAAAAGGAAAGATTTATCTTGAAGAGAAAATAGTTGAGCTCCCACAT
ACTGATAAAATTATGCCTTCTGGTGATGGCCAGTCCCCACTTGCATCGATAAAAGACTGAGTGTTCTTTGAAAAAAATGG
GAAATACTACCGCCGGGAAACAAATACAATGCCACAATGAAGCGGAAGCTCATGGTATTATCTAGCTTTTATTTTAAAAG
AAAAAGATAAATATTTGCCGCTTGATTCAAAAATTGCCTATGATAAATTCAAAAAATGGCTGCCAGTTGATCTTTATATA
GGGGGTCAGGAGCATGCGGTTGGCCATTTAATTTATTCCCGTTTTTGACATAAGGTTTTATTTGATGCGGGGATAGTTCC
AAATTCTGAACCTTTTCAAAAAATAATTCATCAAGGAATGTTACTAGGTTCTGATGGTCAAAAAATGTCGAAATCTAAAG
GAAATACGATAAATCCTACGGAAATGATAGACAAATTTGGTGCTGATACAATTAGAATTTTTTTAATGTTTATGGGTCCG
ATTGGGGATCTTCGACTTTGAGATGAAAATGGAGTAGTTTCAATTTTTAACTGAATTCAACGGGTACAAAGAACAATTTT
AGCTAACTATGAAATTGACCCTGAACTTGAAAAAGACTATGAATTTACGTATTTTTTCAATAATTTTGTTTTCGAAATCA
CAAATTTAATCGAAACTTTTAAGTTCAATGTCGCAATTTCAAAATTAATGATTTACATTAATTTTCTAAATAAAAAGCAA
AAAATTCCTTCAAAAAATTATCTGACAGATTTTTTAATTATTTTCTCAGTTTTTGCACCGCATACAGCAGAAGAATTACT
TGAAAAATTAGGACAAAAACCCTTAAATTTCCAGATTTGACCTCAATTTGATCGGTCAAAAATTTTTAAAAATACTTTTC
ATTTACCTGTTTCAATTAATGGGAAAAACCGCGGAATTCTCGAATTAACAGATGAAAAATCACAAGAAGAAATCATCGAG
ATTGCCAAAAAAGACTATAAAATACGTAACTATTTGCAAAATAAGCAAATAATAAAAGTCATTTATATCCCGAGAAAAAT
TCTAAATTTTATTCTCAAAAATAATTAA

Upstream 100 bases:

>100_bases
AGATCACCGAGCTATTGAAAAAAAGTGACAAGATTACTGGTATAAAAATAAGGTTTTCAAAACAACCGAGACATCTAGAA
AAAAAGCCTATATTCTCGAT

Downstream 100 bases:

>100_bases
AATATGAAAAAATTTTTATTTCCCTTTTCAGTTTCATTTATAAGCTTTTTAGCAGAAATTTTGACCTTGTTATTAAAGGT
TATTTTTTATGATAGTTTTG

Product: leucyl-tRNA synthetase

Products: NA

Alternate protein names: Leucine--tRNA ligase; LeuRS [H]

Number of amino acids: Translated: 755; Mature: 755

Protein sequence:

>755_residues
MFPYPSASGLHLGHPIGYTASDIVARFKRLNGFDVLHPMGWDAFGLPAEQYAIKTGNHPGEFTKRNIENFKKQIISFGFS
YDWDKEINTSDPEFYVQTQWIFKLLYKANLAEIREVEVNWCPELGTVLANEELKREKNGALVSERGNFPVILKKMKQWVL
KITEYAQKLLDGLDQVDFPDSLKALQKKWIGKSKGWKIKFLIEKTGEFLEIFTTKIETFYGVSFLALAPLHNFAQKLAKN
NEKISQFIEQNSFSSPKLQSKMTGIFTGLYAIHPLNSSKIPIYIANYVLNNYATSAIMGVPGHNLADLEFAQIFQIDYVK
VINQENLLINSAEFSGLTIKDGANSIFNKLNSMNLVQKTTIFRLKDWVFSRQRYWGEPFPVYFDEKGKIYLEEKIVELPH
TDKIMPSGDGQSPLASIKDWVFFEKNGKYYRRETNTMPQWSGSSWYYLAFILKEKDKYLPLDSKIAYDKFKKWLPVDLYI
GGQEHAVGHLIYSRFWHKVLFDAGIVPNSEPFQKIIHQGMLLGSDGQKMSKSKGNTINPTEMIDKFGADTIRIFLMFMGP
IGDLRLWDENGVVSIFNWIQRVQRTILANYEIDPELEKDYEFTYFFNNFVFEITNLIETFKFNVAISKLMIYINFLNKKQ
KIPSKNYLTDFLIIFSVFAPHTAEELLEKLGQKPLNFQIWPQFDRSKIFKNTFHLPVSINGKNRGILELTDEKSQEEIIE
IAKKDYKIRNYLQNKQIIKVIYIPRKILNFILKNN

Sequences:

>Translated_755_residues
MFPYPSASGLHLGHPIGYTASDIVARFKRLNGFDVLHPMGWDAFGLPAEQYAIKTGNHPGEFTKRNIENFKKQIISFGFS
YDWDKEINTSDPEFYVQTQWIFKLLYKANLAEIREVEVN*CPELGTVLANEELKREKNGALVSERGNFPVILKKMKQ*VL
KITEYAQKLLDGLDQVDFPDSLKALQKK*IGKSKG*KIKFLIEKTGEFLEIFTTKIETFYGVSFLALAPLHNFAQKLAKN
NEKISQFIEQNSFSSPKLQSKMTGIFTGLYAIHPLNSSKIPIYIANYVLNNYATSAIMGVPGHNLADLEFAQIFQIDYVK
VINQENLLINSAEFSGLTIKDGANSIFNKLNSMNLVQKTTIFRLKD*VFSRQRY*GEPFPVYFDEKGKIYLEEKIVELPH
TDKIMPSGDGQSPLASIKD*VFFEKNGKYYRRETNTMPQ*SGSSWYYLAFILKEKDKYLPLDSKIAYDKFKKWLPVDLYI
GGQEHAVGHLIYSRF*HKVLFDAGIVPNSEPFQKIIHQGMLLGSDGQKMSKSKGNTINPTEMIDKFGADTIRIFLMFMGP
IGDLRL*DENGVVSIFN*IQRVQRTILANYEIDPELEKDYEFTYFFNNFVFEITNLIETFKFNVAISKLMIYINFLNKKQ
KIPSKNYLTDFLIIFSVFAPHTAEELLEKLGQKPLNFQI*PQFDRSKIFKNTFHLPVSINGKNRGILELTDEKSQEEIIE
IAKKDYKIRNYLQNKQIIKVIYIPRKILNFILKNN
>Mature_755_residues
MFPYPSASGLHLGHPIGYTASDIVARFKRLNGFDVLHPMGWDAFGLPAEQYAIKTGNHPGEFTKRNIENFKKQIISFGFS
YDWDKEINTSDPEFYVQTQWIFKLLYKANLAEIREVEVN*CPELGTVLANEELKREKNGALVSERGNFPVILKKMKQ*VL
KITEYAQKLLDGLDQVDFPDSLKALQKK*IGKSKG*KIKFLIEKTGEFLEIFTTKIETFYGVSFLALAPLHNFAQKLAKN
NEKISQFIEQNSFSSPKLQSKMTGIFTGLYAIHPLNSSKIPIYIANYVLNNYATSAIMGVPGHNLADLEFAQIFQIDYVK
VINQENLLINSAEFSGLTIKDGANSIFNKLNSMNLVQKTTIFRLKD*VFSRQRY*GEPFPVYFDEKGKIYLEEKIVELPH
TDKIMPSGDGQSPLASIKD*VFFEKNGKYYRRETNTMPQ*SGSSWYYLAFILKEKDKYLPLDSKIAYDKFKKWLPVDLYI
GGQEHAVGHLIYSRF*HKVLFDAGIVPNSEPFQKIIHQGMLLGSDGQKMSKSKGNTINPTEMIDKFGADTIRIFLMFMGP
IGDLRL*DENGVVSIFN*IQRVQRTILANYEIDPELEKDYEFTYFFNNFVFEITNLIETFKFNVAISKLMIYINFLNKKQ
KIPSKNYLTDFLIIFSVFAPHTAEELLEKLGQKPLNFQI*PQFDRSKIFKNTFHLPVSINGKNRGILELTDEKSQEEIIE
IAKKDYKIRNYLQNKQIIKVIYIPRKILNFILKNN

Specific function: Unknown

COG id: COG0495

COG function: function code J; Leucyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7661872, Length=846, Percent_Identity=29.5508274231678, Blast_Score=298, Evalue=9e-81,
Organism=Escherichia coli, GI1786861, Length=850, Percent_Identity=33.0588235294118, Blast_Score=405, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI71997510, Length=730, Percent_Identity=27.9452054794521, Blast_Score=201, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI71997517, Length=730, Percent_Identity=27.9452054794521, Blast_Score=201, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI212645227, Length=284, Percent_Identity=26.4084507042254, Blast_Score=76, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6323414, Length=854, Percent_Identity=32.903981264637, Blast_Score=317, Evalue=6e-87,
Organism=Drosophila melanogaster, GI21355409, Length=832, Percent_Identity=27.4038461538462, Blast_Score=246, Evalue=5e-65,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002300
- InterPro:   IPR002302
- InterPro:   IPR014729
- InterPro:   IPR009080
- InterPro:   IPR013155
- InterPro:   IPR009008 [H]

Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1 [H]

EC number: =6.1.1.4 [H]

Molecular weight: Translated: 85285; Mature: 85285

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFPYPSASGLHLGHPIGYTASDIVARFKRLNGFDVLHPMGWDAFGLPAEQYAIKTGNHPG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHEEECCCCCC
EFTKRNIENFKKQIISFGFSYDWDKEINTSDPEFYVQTQWIFKLLYKANLAEIREVEVNC
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCHHHHHEEECCC
PELGTVLANEELKREKNGALVSERGNFPVILKKMKQVLKITEYAQKLLDGLDQVDFPDSL
CHHHHHHHCHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
KALQKKIGKSKGKIKFLIEKTGEFLEIFTTKIETFYGVSFLALAPLHNFAQKLAKNNEKI
HHHHHHHCCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
SQFIEQNSFSSPKLQSKMTGIFTGLYAIHPLNSSKIPIYIANYVLNNYATSAIMGVPGHN
HHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCEEEHHHHHHHHHHHHEEECCCCC
LADLEFAQIFQIDYVKVINQENLLINSAEFSGLTIKDGANSIFNKLNSMNLVQKTTIFRL
CCCCHHHHHHHHHHHEEECCCCEEEECCCCCCEEEECCHHHHHHHHHCCHHHHHHHHHHH
KDVFSRQRYGEPFPVYFDEKGKIYLEEKIVELPHTDKIMPSGDGQSPLASIKDVFFEKNG
HHHHHHHHCCCCCCEEECCCCCEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
KYYRRETNTMPQSGSSWYYLAFILKEKDKYLPLDSKIAYDKFKKWLPVDLYIGGQEHAVG
CEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEEECCCHHHHH
HLIYSRFHKVLFDAGIVPNSEPFQKIIHQGMLLGSDGQKMSKSKGNTINPTEMIDKFGAD
HHHHHHHHHHHHHCCCCCCCHHHHHHHHCCEEECCCCCHHHHCCCCCCCHHHHHHHHCCH
TIRIFLMFMGPIGDLRLDENGVVSIFNIQRVQRTILANYEIDPELEKDYEFTYFFNNFVF
HHHHHHHHHCCCCCCEECCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHH
EITNLIETFKFNVAISKLMIYINFLNKKQKIPSKNYLTDFLIIFSVFAPHTAEELLEKLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHC
QKPLNFQIPQFDRSKIFKNTFHLPVSINGKNRGILELTDEKSQEEIIEIAKKDYKIRNYL
CCCCCCCCCCCCHHHHHHHHEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
QNKQIIKVIYIPRKILNFILKNN
CCCCEEEEEEECHHHHHHHHCCC
>Mature Secondary Structure
MFPYPSASGLHLGHPIGYTASDIVARFKRLNGFDVLHPMGWDAFGLPAEQYAIKTGNHPG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHEEECCCCCC
EFTKRNIENFKKQIISFGFSYDWDKEINTSDPEFYVQTQWIFKLLYKANLAEIREVEVNC
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHCCHHHHHEEECCC
PELGTVLANEELKREKNGALVSERGNFPVILKKMKQVLKITEYAQKLLDGLDQVDFPDSL
CHHHHHHHCHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
KALQKKIGKSKGKIKFLIEKTGEFLEIFTTKIETFYGVSFLALAPLHNFAQKLAKNNEKI
HHHHHHHCCCCCCEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
SQFIEQNSFSSPKLQSKMTGIFTGLYAIHPLNSSKIPIYIANYVLNNYATSAIMGVPGHN
HHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCEEEHHHHHHHHHHHHEEECCCCC
LADLEFAQIFQIDYVKVINQENLLINSAEFSGLTIKDGANSIFNKLNSMNLVQKTTIFRL
CCCCHHHHHHHHHHHEEECCCCEEEECCCCCCEEEECCHHHHHHHHHCCHHHHHHHHHHH
KDVFSRQRYGEPFPVYFDEKGKIYLEEKIVELPHTDKIMPSGDGQSPLASIKDVFFEKNG
HHHHHHHHCCCCCCEEECCCCCEEEEEHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCC
KYYRRETNTMPQSGSSWYYLAFILKEKDKYLPLDSKIAYDKFKKWLPVDLYIGGQEHAVG
CEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHCCCCEEEEECCCHHHHH
HLIYSRFHKVLFDAGIVPNSEPFQKIIHQGMLLGSDGQKMSKSKGNTINPTEMIDKFGAD
HHHHHHHHHHHHHCCCCCCCHHHHHHHHCCEEECCCCCHHHHCCCCCCCHHHHHHHHCCH
TIRIFLMFMGPIGDLRLDENGVVSIFNIQRVQRTILANYEIDPELEKDYEFTYFFNNFVF
HHHHHHHHHCCCCCCEECCCCCEEEEEHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHH
EITNLIETFKFNVAISKLMIYINFLNKKQKIPSKNYLTDFLIIFSVFAPHTAEELLEKLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHC
QKPLNFQIPQFDRSKIFKNTFHLPVSINGKNRGILELTDEKSQEEIIEIAKKDYKIRNYL
CCCCCCCCCCCCHHHHHHHHEEEEEEECCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
QNKQIIKVIYIPRKILNFILKNN
CCCCEEEEEEECHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11353084 [H]