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The map label for this gene is hphIAM [H]

Identifier: 54020626

GI number: 54020626

Start: 791932

End: 793182

Strand: Reverse

Name: hphIAM [H]

Synonym: mhp633

Alternate gene names: 54020626

Gene position: 793182-791932 (Counterclockwise)

Preceding gene: 54020627

Following gene: 54020625

Centisome position: 88.85

GC content: 32.45

Gene sequence:

>1251_bases
ATGAAATACTATAAAATTTCCGAAATTGCAAACCTTGAAGCTGTTAGTCAAAAATTCATTAGAAGAGAAATTGCAAGAAA
AACTTTAATCTCAATAAAGAAAAATAATAAGTATCTAATTTCGGAATTTGATTATCAAAAATGAAAATCATCCTATAGAA
AACAAGAAAAACTAAGGAAAAATTTAGGAAAAACTAAAGAAAAAGTCCACTTTATTGAGATCTCAGAAGAAATGAAGAAA
CTTGATGGTTGGAGCTCAAGTTATCACAATGGTTATAATTTTATCGATCTTTTCTCGGGTGCTGGCGGCCTTTCTTGTGG
TCTTGTTATGGCTGGATTTTTGCCCTTGGCAAGTTTAGAAATAATGAAACAAGCCTTTGAAACCTATGCCTATAATTTTA
AAAAAAGGTCCAAAAATAAAGAACTTTTTAAACTAGGCGATATTAGAGATTCTAAAATAAAATCCGAATTTTATGATCAT
TTTAAGGATCAAGAACTGGATTTAATTGCTGGAGGTTTTCCTTGTCAGGGCTTTTCTATGGCAGGTAATCGAGTCTTTGA
TGATCCCCGAAATAATTTATACCTTGAAATGTTAGATATTGTTGCAAATTTAAAGCCAAAATTCGTGTTAATGGAGAATG
TTCAAGGGCTTAGAACCATGCTTTCTGGTCAAATTGAGGCCAAAATCATAAAAGATTTTGAAAAAATCGGCTATAAGATT
AATGTAGCAACATTAAATAGTGCCGATTTTGGAGTCGCTCAAATAAGAAAACGGGTAATTTTTATTGCAAACCGGATTGG
TTTGACAAATTTTTTCCCTGCAGCTATTCTTAGCCCAGAAAATTATAAAACTTTAGGTCAGTGTATTGAAAAATATATGA
ATTGAGAAGAAAATATTGAAATTAATCATATTTTTACAAAACATTCAGCCAAAATGAGGGAAAAAATTTTAAAAACATCC
GAAGGACAAAGCGTTTATAAAAATTACTCTGATGGTTGGAAGAAATCACCTTGAAATCAGCCTAGTTGTACAGTTAAAGA
AAATCATGGCGGGGTGAATCTCCATCCTAAACTCCCAAGGGTTTTAACTCCGCGTGAGTTGGCGGCTTTACAGTCATTTC
CTGATAGTTTTATTTTTAAAGGGTCAAAAAAATGACAACTTGTGCAAATCGGAAATGCGGTGCCCCCACTTTTAGCAAAG
GCTATCGGCCTGGCTATTAAAAAATCTTTGGATAAGAAAGATGTATTATAA

Upstream 100 bases:

>100_bases
GATCTAGAGACCAGATCGGTGAAAATCTGGTGAAATTAACTAAATTTTTCCTTTTTTTGATAAAATAATTTAAAAAAAAT
TAATATATAATAGACTAGAA

Downstream 100 bases:

>100_bases
GGTTTTAAAATTTTTCCAGAAACAAATTGAAGATTATGCCAAACAAATCTTTAAGGTTTATGGAAATGAATTAACCTTAA
AATTAGAAGATTATAATTTA

Product: cytosine specific DNA methyltransferase

Products: NA

Alternate protein names: M.HphIA; Cytosine-specific methyltransferase HphIA; M.Hphi(C) [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MKYYKISEIANLEAVSQKFIRREIARKTLISIKKNNKYLISEFDYQKWKSSYRKQEKLRKNLGKTKEKVHFIEISEEMKK
LDGWSSSYHNGYNFIDLFSGAGGLSCGLVMAGFLPLASLEIMKQAFETYAYNFKKRSKNKELFKLGDIRDSKIKSEFYDH
FKDQELDLIAGGFPCQGFSMAGNRVFDDPRNNLYLEMLDIVANLKPKFVLMENVQGLRTMLSGQIEAKIIKDFEKIGYKI
NVATLNSADFGVAQIRKRVIFIANRIGLTNFFPAAILSPENYKTLGQCIEKYMNWEENIEINHIFTKHSAKMREKILKTS
EGQSVYKNYSDGWKKSPWNQPSCTVKENHGGVNLHPKLPRVLTPRELAALQSFPDSFIFKGSKKWQLVQIGNAVPPLLAK
AIGLAIKKSLDKKDVL

Sequences:

>Translated_416_residues
MKYYKISEIANLEAVSQKFIRREIARKTLISIKKNNKYLISEFDYQK*KSSYRKQEKLRKNLGKTKEKVHFIEISEEMKK
LDGWSSSYHNGYNFIDLFSGAGGLSCGLVMAGFLPLASLEIMKQAFETYAYNFKKRSKNKELFKLGDIRDSKIKSEFYDH
FKDQELDLIAGGFPCQGFSMAGNRVFDDPRNNLYLEMLDIVANLKPKFVLMENVQGLRTMLSGQIEAKIIKDFEKIGYKI
NVATLNSADFGVAQIRKRVIFIANRIGLTNFFPAAILSPENYKTLGQCIEKYMN*EENIEINHIFTKHSAKMREKILKTS
EGQSVYKNYSDGWKKSP*NQPSCTVKENHGGVNLHPKLPRVLTPRELAALQSFPDSFIFKGSKK*QLVQIGNAVPPLLAK
AIGLAIKKSLDKKDVL
>Mature_416_residues
MKYYKISEIANLEAVSQKFIRREIARKTLISIKKNNKYLISEFDYQK*KSSYRKQEKLRKNLGKTKEKVHFIEISEEMKK
LDGWSSSYHNGYNFIDLFSGAGGLSCGLVMAGFLPLASLEIMKQAFETYAYNFKKRSKNKELFKLGDIRDSKIKSEFYDH
FKDQELDLIAGGFPCQGFSMAGNRVFDDPRNNLYLEMLDIVANLKPKFVLMENVQGLRTMLSGQIEAKIIKDFEKIGYKI
NVATLNSADFGVAQIRKRVIFIANRIGLTNFFPAAILSPENYKTLGQCIEKYMN*EENIEINHIFTKHSAKMREKILKTS
EGQSVYKNYSDGWKKSP*NQPSCTVKENHGGVNLHPKLPRVLTPRELAALQSFPDSFIFKGSKK*QLVQIGNAVPPLLAK
AIGLAIKKSLDKKDVL

Specific function: This methylase protects the DNA from cleavage by the HphI endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4503351, Length=184, Percent_Identity=27.7173913043478, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI195927037, Length=184, Percent_Identity=27.7173913043478, Blast_Score=73, Evalue=4e-13,
Organism=Escherichia coli, GI1788271, Length=343, Percent_Identity=24.198250728863, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 46932; Mature: 46932

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS00094 C5_MTASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYYKISEIANLEAVSQKFIRREIARKTLISIKKNNKYLISEFDYQKKSSYRKQEKLRKN
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCHHHHHHHHHHHHHHH
LGKTKEKVHFIEISEEMKKLDGWSSSYHNGYNFIDLFSGAGGLSCGLVMAGFLPLASLEI
HCCHHHHEEEEEHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
MKQAFETYAYNFKKRSKNKELFKLGDIRDSKIKSEFYDHFKDQELDLIAGGFPCQGFSMA
HHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
GNRVFDDPRNNLYLEMLDIVANLKPKFVLMENVQGLRTMLSGQIEAKIIKDFEKIGYKIN
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEE
VATLNSADFGVAQIRKRVIFIANRIGLTNFFPAAILSPENYKTLGQCIEKYMNEENIEIN
EEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCEEE
HIFTKHSAKMREKILKTSEGQSVYKNYSDGWKKSPNQPSCTVKENHGGVNLHPKLPRVLT
EEEHHHHHHHHHHHHHCCCCHHHHHCCCCHHCCCCCCCCCEEECCCCCEEECCCCCCCCC
PRELAALQSFPDSFIFKGSKKQLVQIGNAVPPLLAKAIGLAIKKSLDKKDVL
HHHHHHHHHCCHHHEECCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKYYKISEIANLEAVSQKFIRREIARKTLISIKKNNKYLISEFDYQKKSSYRKQEKLRKN
CCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEECCCCHHHHHHHHHHHHHHH
LGKTKEKVHFIEISEEMKKLDGWSSSYHNGYNFIDLFSGAGGLSCGLVMAGFLPLASLEI
HCCHHHHEEEEEHHHHHHHHCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHH
MKQAFETYAYNFKKRSKNKELFKLGDIRDSKIKSEFYDHFKDQELDLIAGGFPCQGFSMA
HHHHHHHHHHHHHHHCCCCCCEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCCCC
GNRVFDDPRNNLYLEMLDIVANLKPKFVLMENVQGLRTMLSGQIEAKIIKDFEKIGYKIN
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEE
VATLNSADFGVAQIRKRVIFIANRIGLTNFFPAAILSPENYKTLGQCIEKYMNEENIEIN
EEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCCEEE
HIFTKHSAKMREKILKTSEGQSVYKNYSDGWKKSPNQPSCTVKENHGGVNLHPKLPRVLT
EEEHHHHHHHHHHHHHCCCCHHHHHCCCCHHCCCCCCCCCEEECCCCCEEECCCCCCCCC
PRELAALQSFPDSFIFKGSKKQLVQIGNAVPPLLAKAIGLAIKKSLDKKDVL
HHHHHHHHHCCHHHEECCCHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8759008 [H]