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The map label for this gene is spoU

Identifier: 54020406

GI number: 54020406

Start: 264379

End: 264903

Strand: Reverse

Name: spoU

Synonym: mhp239

Alternate gene names: 54020406

Gene position: 264903-264379 (Counterclockwise)

Preceding gene: 54020407

Following gene: 54020405

Centisome position: 29.67

GC content: 30.86

Gene sequence:

>525_bases
ATGATTAATATTGTTTTATTTCAACCCGAAATCGGCCCTAATACCGGCAATATAATTCGTTCTTGTTTTGTTGCTGGACT
AAAACTGCATATTATTATGCCGATTGCCTTTGATTTAGACCCAAAACACTTAAAAAGACCGGCTGCGGGAATTTTATTAT
CTGAGATTGAGCATGAGATACATAAAAATTGACAAAATTTTTCTGAAAAATACGGTCAAAAAAATCTATTTTTTATTACC
CGTTATGGCCAAAAATTGTACACTGAAGTTGATTTTTACACTGAAATCGAAACAAAAAAAGATATTTTTTTTGTTTTTGG
CCGTGAATCTACCGGCATTCCAATTGAAATTTTGAAAAAAAACAAAGAAAAATGCCTCAGAATACCAATGATCGCGAAAG
CTCGTTCATTAAATTTAGCAAACACAGTTGTAATTATAGCATATGAATTCCACAGACAATTAAACTTCAAAAAATTATCA
CGATTCGAGGTACAAAAAGGAACAAATTATCTTGATGAGAATTAA

Upstream 100 bases:

>100_bases
AATTTAATTTTGCTTGAATATGATCTTTTTCTAAAAGGAAATAATAATAATAATAATAATAATAAAATTGCCACTTATAT
AACAAATATGGAACTAATAA

Downstream 100 bases:

>100_bases
AGAAATTACTTCTTATAATAATCCAATTATAAAAGAAATAGGTAAATTAAAATTAAAAAAATACCGTAATTTATGGAATA
AATTTGTAGTTGAAGGTGAA

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 174; Mature: 174

Protein sequence:

>174_residues
MINIVLFQPEIGPNTGNIIRSCFVAGLKLHIIMPIAFDLDPKHLKRPAAGILLSEIEHEIHKNWQNFSEKYGQKNLFFIT
RYGQKLYTEVDFYTEIETKKDIFFVFGRESTGIPIEILKKNKEKCLRIPMIAKARSLNLANTVVIIAYEFHRQLNFKKLS
RFEVQKGTNYLDEN

Sequences:

>Translated_174_residues
MINIVLFQPEIGPNTGNIIRSCFVAGLKLHIIMPIAFDLDPKHLKRPAAGILLSEIEHEIHKN*QNFSEKYGQKNLFFIT
RYGQKLYTEVDFYTEIETKKDIFFVFGRESTGIPIEILKKNKEKCLRIPMIAKARSLNLANTVVIIAYEFHRQLNFKKLS
RFEVQKGTNYLDEN
>Mature_174_residues
MINIVLFQPEIGPNTGNIIRSCFVAGLKLHIIMPIAFDLDPKHLKRPAAGILLSEIEHEIHKN*QNFSEKYGQKNLFFIT
RYGQKLYTEVDFYTEIETKKDIFFVFGRESTGIPIEILKKNKEKCLRIPMIAKARSLNLANTVVIIAYEFHRQLNFKKLS
RFEVQKGTNYLDEN

Specific function: Could methylate the ribose at the nucleotide 34 wobble position in tRNA [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=158, Percent_Identity=39.2405063291139, Blast_Score=111, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 20102; Mature: 20102

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINIVLFQPEIGPNTGNIIRSCFVAGLKLHIIMPIAFDLDPKHLKRPAAGILLSEIEHEI
CEEEEEECCCCCCCHHHHHHHHHHHCEEEEEEEEEEECCCHHHHCCCHHHHHHHHHHHHH
HKNQNFSEKYGQKNLFFITRYGQKLYTEVDFYTEIETKKDIFFVFGRESTGIPIEILKKN
HCCCCCHHHHCCCCEEEEEECCHHHHHEEHEEEECCCCCEEEEEECCCCCCCCHHEECCC
KEKCLRIPMIAKARSLNLANTVVIIAYEFHRQLNFKKLSRFEVQKGTNYLDEN
CHHHEECCHHHHHCCCCCCCEEEEEEEEHHCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MINIVLFQPEIGPNTGNIIRSCFVAGLKLHIIMPIAFDLDPKHLKRPAAGILLSEIEHEI
CEEEEEECCCCCCCHHHHHHHHHHHCEEEEEEEEEEECCCHHHHCCCHHHHHHHHHHHHH
HKNQNFSEKYGQKNLFFITRYGQKLYTEVDFYTEIETKKDIFFVFGRESTGIPIEILKKN
HCCCCCHHHHCCCCEEEEEECCHHHHHEEHEEEECCCCCEEEEEECCCCCCCCHHEECCC
KEKCLRIPMIAKARSLNLANTVVIIAYEFHRQLNFKKLSRFEVQKGTNYLDEN
CHHHEECCHHHHHCCCCCCCEEEEEEEEHHCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1339360 [H]