| Definition | Mycoplasma hyopneumoniae 232, complete genome. |
|---|---|
| Accession | NC_006360 |
| Length | 892,758 |
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The map label for this gene is valS
Identifier: 54020305
GI number: 54020305
Start: 884003
End: 886471
Strand: Direct
Name: valS
Synonym: mhp694
Alternate gene names: 54020305
Gene position: 884003-886471 (Clockwise)
Preceding gene: 54020304
Following gene: 54020306
Centisome position: 99.02
GC content: 29.93
Gene sequence:
>2469_bases ATGAAAAATAAATATGATTTTAAGCTTGTAGAAGAAAAAAGAAATGAAAAATGACAAAAAAAAGGCTTTTTTATCGCCCC AAAACAAACAAAAAAGCCTTTTTCAATTATTTCTCCTCCGCCAAATGTAACCGGACAACTCCATCTTGGGCACTCTTGAA ACGCTTTTATCCAGGATTCATTAGTCCGTTACCACAAATTACAAGGTTTTGATGTTCTGCTTCTACCCAGTGTCGATCAT GCCGGAATTGCCACGCAAGTAAAAGTTGAAGAGGATTTAGCAAAAAAAGGGATTAAAAAATCTGATCTTAAAAGAGAAGA ATTTATAAAAAAGTGTTATCACTGAAAAGAAAAACAATATTTAAAAATCAAAGAACAATGAGATAAACTGGGAATTTGTT ATGATTTTTCCAAGGAAAGATTCACCCTAGATCAGGATGCTCAAATTGCTGTAAGTGATTTTTTTATCAAATTATGAGAA AAAAATCTAATTTATCGCGGGCAAAAAGCGATAAATTGAGATATAAAATTACAGACTGCAATTTCTAATATCGAGGTGAT CAACAAACCTGTTGAACAAAAAATGTACTATTTAAAGTACTTTTTGGAAAATTCTAATGAATTTTTAACAGTTGCTACTA CCCGAATCGAAACGATATCCTCTGATGTTGCCCTGGCAATAAACCCAAAAGATAAAAGATATTTACACCTGGTTGGCAAG AAAGTAGTCCACCCGCTAACAAAAAAGTTAATCAAAATTATTGCTGATTCAAATGTTGGTTCCGATTTTGGATCTGGGAT AATGAAAGTTTCAGCCCACTCAATTCTTGACTTTGAAATTATGGAAAAACATAATTTAGAATCAAAAGATTGTATTGATA ATTATGGTAACTTAAATCACGAAGTGCCAGAATTTCAAGGTCAAAATCGTTTTTTTGCCCGCGACTTAATTGCAAAAAAA CTTGAAAAAGAAGGATTTTTAGCTAAAATAGAAACTGTTATTTCAAATGTTGGTTTTTCCCAACGCAGTGATGAAATTGT TGAAATTCTAAAAAAACCACAGTGATTTGTAAAAATGGATGAACTTGCTAAAAGTTTAATTTCGCACTTAAATTCAAAAG ATAAAATTAAATTCTACCCCAAGAATTTTGAAAAAAACCTGCGAAAATGATTTGAAAAAATTCATGACTGAACGATTTCA CGGCAACTTTGATGGGGCCACAGAATTCCTGTTTGATATAAAAATGACGAATTTAAAGTACAAATTGACTCACCTGGTCA GGGTTGAATTCAGGATGAAGATGTTCTTGATACTTGGTTTTCTTCCGGAATTAGTGTTTTTGCTTTTCTTGGTTGACCAC AGAATTTCGATCTAATTAAATCATATTTTCCGACAAGTTTACTCGTGACTGGCTGGGATATTTTATTTTTTTGGGTAGCG AGAATGTATTTTTCATCACTTTTTATAATGAAACAAAAGCCTTTTGAAAAGGTTTTGCTCCACGGTTTAATCCGGGATGA AATTGGGAGAAAAATGTCAAAATCTCTCGGAAATGGCTTAGATCCGATGGAAATAATTGAAAAATATGGCTCAGATACGC TAAGGCAGGCATTAATTTTCAATTCGAGTCCTGGAAAAGACATCAAATTCAACATTGAAAAGTTAAATACCGCCTGAAAT TTGAATAATAAAATCTGAAACATTGCAAAATATATTGCAGATTTAGACACTTTTTTTGCAAAACCTGACCTTATTGACCT TTGAATGGAAAATAAAATTTATATTTTAAAGCGGCAAATAGTAAAAAATATAAAAAAATATAATTTTAGCGTGATTGGAA CTGAAATAAATAATTTTATCTACGGGGATTTTTCTTCGCGTTATATTGAATTAATTAAAACGCGCAAAAACGGGTTTTAT GCTCGCAAACTTTTGAGAAAAGTTTTAATTATTTTACACCCGTTTTTACCATTTTTAACTGATTTCTTGATGGAAAAAAT TTTCAAAATGGAAATTTTAGAACAAAAAATGCCTAGAATTAGACAGTTTAAAGAAAACCAAAAAGTTGAAAATATCCTTG AAATTATAGATAATTTGCGAACTTATAGAGAAAAATTTCAAATTTCTAAAAAAATAATTTTAGAATATTGTATAATTAAT GACAAGTTTTCTAATGCTGAAATCGACATCATAAACAAACTCACCTTCGGAAAATGGCTAGAAAATAAAGAGTTAGTAAT TAAAACAAAAAATTTTGAAATCGCAATTAAAGTTCCTGAAGAATTGAAAAAAGAACAAAAAGGGCGTGAATTAAAAGAAA TTCAATTTCTTAAATCAGAAATTTTAAGGGCTGAAAAAATCCTTACAAATAAAGGATTTTTAGAAAAAGCTCCTAGAGAA AAAATAGATTTAGAGCGAACTAAACTTGAAAAATTAAAGGAAAAATTAGCTTTTTATGAGAAAAAGTAA
Upstream 100 bases:
>100_bases TGAATTCCAATAAAAGAATTTAAACTCGAATTTAATAGTGAGAATAAAAAATATTCGCTAAAAAAAGATATTCTTGTAAT TGATTTATAAAAGGATTTTT
Downstream 100 bases:
>100_bases AGCTTTTTATTCAGATTTTTCAATTAAAATTGAAAAATCTGAATCAAATTCTTCTTCCTTTCCTTCAAAAGGAACAATAA ATGTTTGAGTATAAAATCTA
Product: valyl-tRNA synthetase
Products: NA
Alternate protein names: Valine--tRNA ligase; ValRS [H]
Number of amino acids: Translated: 822; Mature: 822
Protein sequence:
>822_residues MKNKYDFKLVEEKRNEKWQKKGFFIAPKQTKKPFSIISPPPNVTGQLHLGHSWNAFIQDSLVRYHKLQGFDVLLLPSVDH AGIATQVKVEEDLAKKGIKKSDLKREEFIKKCYHWKEKQYLKIKEQWDKLGICYDFSKERFTLDQDAQIAVSDFFIKLWE KNLIYRGQKAINWDIKLQTAISNIEVINKPVEQKMYYLKYFLENSNEFLTVATTRIETISSDVALAINPKDKRYLHLVGK KVVHPLTKKLIKIIADSNVGSDFGSGIMKVSAHSILDFEIMEKHNLESKDCIDNYGNLNHEVPEFQGQNRFFARDLIAKK LEKEGFLAKIETVISNVGFSQRSDEIVEILKKPQWFVKMDELAKSLISHLNSKDKIKFYPKNFEKNLRKWFEKIHDWTIS RQLWWGHRIPVWYKNDEFKVQIDSPGQGWIQDEDVLDTWFSSGISVFAFLGWPQNFDLIKSYFPTSLLVTGWDILFFWVA RMYFSSLFIMKQKPFEKVLLHGLIRDEIGRKMSKSLGNGLDPMEIIEKYGSDTLRQALIFNSSPGKDIKFNIEKLNTAWN LNNKIWNIAKYIADLDTFFAKPDLIDLWMENKIYILKRQIVKNIKKYNFSVIGTEINNFIYGDFSSRYIELIKTRKNGFY ARKLLRKVLIILHPFLPFLTDFLMEKIFKMEILEQKMPRIRQFKENQKVENILEIIDNLRTYREKFQISKKIILEYCIIN DKFSNAEIDIINKLTFGKWLENKELVIKTKNFEIAIKVPEELKKEQKGRELKEIQFLKSEILRAEKILTNKGFLEKAPRE KIDLERTKLEKLKEKLAFYEKK
Sequences:
>Translated_822_residues MKNKYDFKLVEEKRNEK*QKKGFFIAPKQTKKPFSIISPPPNVTGQLHLGHS*NAFIQDSLVRYHKLQGFDVLLLPSVDH AGIATQVKVEEDLAKKGIKKSDLKREEFIKKCYH*KEKQYLKIKEQ*DKLGICYDFSKERFTLDQDAQIAVSDFFIKL*E KNLIYRGQKAIN*DIKLQTAISNIEVINKPVEQKMYYLKYFLENSNEFLTVATTRIETISSDVALAINPKDKRYLHLVGK KVVHPLTKKLIKIIADSNVGSDFGSGIMKVSAHSILDFEIMEKHNLESKDCIDNYGNLNHEVPEFQGQNRFFARDLIAKK LEKEGFLAKIETVISNVGFSQRSDEIVEILKKPQ*FVKMDELAKSLISHLNSKDKIKFYPKNFEKNLRK*FEKIHD*TIS RQL*WGHRIPV*YKNDEFKVQIDSPGQG*IQDEDVLDTWFSSGISVFAFLG*PQNFDLIKSYFPTSLLVTGWDILFFWVA RMYFSSLFIMKQKPFEKVLLHGLIRDEIGRKMSKSLGNGLDPMEIIEKYGSDTLRQALIFNSSPGKDIKFNIEKLNTA*N LNNKI*NIAKYIADLDTFFAKPDLIDL*MENKIYILKRQIVKNIKKYNFSVIGTEINNFIYGDFSSRYIELIKTRKNGFY ARKLLRKVLIILHPFLPFLTDFLMEKIFKMEILEQKMPRIRQFKENQKVENILEIIDNLRTYREKFQISKKIILEYCIIN DKFSNAEIDIINKLTFGKWLENKELVIKTKNFEIAIKVPEELKKEQKGRELKEIQFLKSEILRAEKILTNKGFLEKAPRE KIDLERTKLEKLKEKLAFYEKK >Mature_822_residues MKNKYDFKLVEEKRNEK*QKKGFFIAPKQTKKPFSIISPPPNVTGQLHLGHS*NAFIQDSLVRYHKLQGFDVLLLPSVDH AGIATQVKVEEDLAKKGIKKSDLKREEFIKKCYH*KEKQYLKIKEQ*DKLGICYDFSKERFTLDQDAQIAVSDFFIKL*E KNLIYRGQKAIN*DIKLQTAISNIEVINKPVEQKMYYLKYFLENSNEFLTVATTRIETISSDVALAINPKDKRYLHLVGK KVVHPLTKKLIKIIADSNVGSDFGSGIMKVSAHSILDFEIMEKHNLESKDCIDNYGNLNHEVPEFQGQNRFFARDLIAKK LEKEGFLAKIETVISNVGFSQRSDEIVEILKKPQ*FVKMDELAKSLISHLNSKDKIKFYPKNFEKNLRK*FEKIHD*TIS RQL*WGHRIPV*YKNDEFKVQIDSPGQG*IQDEDVLDTWFSSGISVFAFLG*PQNFDLIKSYFPTSLLVTGWDILFFWVA RMYFSSLFIMKQKPFEKVLLHGLIRDEIGRKMSKSLGNGLDPMEIIEKYGSDTLRQALIFNSSPGKDIKFNIEKLNTA*N LNNKI*NIAKYIADLDTFFAKPDLIDL*MENKIYILKRQIVKNIKKYNFSVIGTEINNFIYGDFSSRYIELIKTRKNGFY ARKLLRKVLIILHPFLPFLTDFLMEKIFKMEILEQKMPRIRQFKENQKVENILEIIDNLRTYREKFQISKKIILEYCIIN DKFSNAEIDIINKLTFGKWLENKELVIKTKNFEIAIKVPEELKKEQKGRELKEIQFLKSEILRAEKILTNKGFLEKAPRE KIDLERTKLEKLKEKLAFYEKK
Specific function: Catalyzes the attachment of valine to tRNA(Val). As ValRS can inadvertently accommodate and process structurally similar amino acids such as threonine, to avoid such errors, it has a "posttransfer" editing activity that hydrolyzes mischarged Thr-tRNA(Val)
COG id: COG0525
COG function: function code J; Valyl-tRNA synthetase
Gene ontology:
Cell location: Cytoplasm [H]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. ValS type 1 subfamily [H]
Homologues:
Organism=Homo sapiens, GI5454158, Length=795, Percent_Identity=32.5786163522013, Blast_Score=351, Evalue=2e-96, Organism=Homo sapiens, GI268370297, Length=825, Percent_Identity=29.8181818181818, Blast_Score=333, Evalue=3e-91, Organism=Homo sapiens, GI268370293, Length=825, Percent_Identity=29.8181818181818, Blast_Score=333, Evalue=3e-91, Organism=Homo sapiens, GI268370295, Length=786, Percent_Identity=30.1526717557252, Blast_Score=325, Evalue=1e-88, Organism=Homo sapiens, GI46852147, Length=799, Percent_Identity=22.6533166458073, Blast_Score=118, Evalue=2e-26, Organism=Escherichia coli, GI1790708, Length=944, Percent_Identity=31.1440677966102, Blast_Score=375, Evalue=1e-105, Organism=Escherichia coli, GI2367096, Length=730, Percent_Identity=23.972602739726, Blast_Score=137, Evalue=3e-33, Organism=Caenorhabditis elegans, GI17510661, Length=803, Percent_Identity=31.1332503113325, Blast_Score=335, Evalue=6e-92, Organism=Caenorhabditis elegans, GI72001587, Length=782, Percent_Identity=30.0511508951407, Blast_Score=300, Evalue=1e-81, Organism=Caenorhabditis elegans, GI17541896, Length=895, Percent_Identity=22.7932960893855, Blast_Score=115, Evalue=8e-26, Organism=Caenorhabditis elegans, GI71980946, Length=284, Percent_Identity=23.2394366197183, Blast_Score=75, Evalue=1e-13, Organism=Saccharomyces cerevisiae, GI6321531, Length=739, Percent_Identity=35.0473612990528, Blast_Score=346, Evalue=9e-96, Organism=Saccharomyces cerevisiae, GI6319395, Length=841, Percent_Identity=24.0190249702735, Blast_Score=102, Evalue=3e-22, Organism=Drosophila melanogaster, GI17864482, Length=852, Percent_Identity=31.4553990610329, Blast_Score=342, Evalue=6e-94, Organism=Drosophila melanogaster, GI24653289, Length=852, Percent_Identity=31.4553990610329, Blast_Score=342, Evalue=6e-94, Organism=Drosophila melanogaster, GI21355675, Length=791, Percent_Identity=29.582806573957, Blast_Score=287, Evalue=2e-77, Organism=Drosophila melanogaster, GI28574730, Length=773, Percent_Identity=22.8978007761966, Blast_Score=103, Evalue=6e-22, Organism=Drosophila melanogaster, GI24668547, Length=773, Percent_Identity=22.8978007761966, Blast_Score=103, Evalue=6e-22, Organism=Drosophila melanogaster, GI24668543, Length=773, Percent_Identity=22.8978007761966, Blast_Score=103, Evalue=6e-22, Organism=Drosophila melanogaster, GI281366294, Length=753, Percent_Identity=21.9123505976096, Blast_Score=102, Evalue=8e-22,
Paralogues:
None
Copy number: 120 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 800 Molecules/Cell In: Glucose minimal media [C]
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR001412 - InterPro: IPR002300 - InterPro: IPR014729 - InterPro: IPR009080 - InterPro: IPR013155 - InterPro: IPR019499 - InterPro: IPR009008 - InterPro: IPR002303 [H]
Pfam domain/function: PF08264 Anticodon_1; PF00133 tRNA-synt_1; PF10458 Val_tRNA-synt_C [H]
EC number: =6.1.1.9 [H]
Molecular weight: Translated: 93986; Mature: 93986
Theoretical pI: Translated: 10.02; Mature: 10.02
Prosite motif: PS00178 AA_TRNA_LIGASE_I
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.5 %Cys (Translated Protein) 1.6 %Met (Translated Protein) 2.1 %Cys+Met (Translated Protein) 0.5 %Cys (Mature Protein) 1.6 %Met (Mature Protein) 2.1 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKNKYDFKLVEEKRNEKQKKGFFIAPKQTKKPFSIISPPPNVTGQLHLGHSNAFIQDSLV CCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCEEECCCCCCCEEEEECCCCCHHHHHHH RYHKLQGFDVLLLPSVDHAGIATQVKVEEDLAKKGIKKSDLKREEFIKKCYHKEKQYLKI HHHHCCCCCEEEECCCCCCCCEEEEEEHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHEEE KEQDKLGICYDFSKERFTLDQDAQIAVSDFFIKLEKNLIYRGQKAINDIKLQTAISNIEV ECCCCCEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH INKPVEQKMYYLKYFLENSNEFLTVATTRIETISSDVALAINPKDKRYLHLVGKKVVHPL HCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH TKKLIKIIADSNVGSDFGSGIMKVSAHSILDFEIMEKHNLESKDCIDNYGNLNHEVPEFQ HHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCC GQNRFFARDLIAKKLEKEGFLAKIETVISNVGFSQRSDEIVEILKKPQFVKMDELAKSLI CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHH SHLNSKDKIKFYPKNFEKNLRKFEKIHDTISRQLWGHRIPVYKNDEFKVQIDSPGQGIQD HHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCC EDVLDTWFSSGISVFAFLGPQNFDLIKSYFPTSLLVTGWDILFFWVARMYFSSLFIMKQK HHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC PFEKVLLHGLIRDEIGRKMSKSLGNGLDPMEIIEKYGSDTLRQALIFNSSPGKDIKFNIE CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEE KLNTANLNNKINIAKYIADLDTFFAKPDLIDLMENKIYILKRQIVKNIKKYNFSVIGTEI ECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEEC NNFIYGDFSSRYIELIKTRKNGFYARKLLRKVLIILHPFLPFLTDFLMEKIFKMEILEQK CCCEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH MPRIRQFKENQKVENILEIIDNLRTYREKFQISKKIILEYCIINDKFSNAEIDIINKLTF HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHH GKWLENKELVIKTKNFEIAIKVPEELKKEQKGRELKEIQFLKSEILRAEKILTNKGFLEK HHHCCCCEEEEEECCEEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHH APREKIDLERTKLEKLKEKLAFYEKK CCCHHHHHHHHHHHHHHHHHHHHCCC >Mature Secondary Structure MKNKYDFKLVEEKRNEKQKKGFFIAPKQTKKPFSIISPPPNVTGQLHLGHSNAFIQDSLV CCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCEEECCCCCCCEEEEECCCCCHHHHHHH RYHKLQGFDVLLLPSVDHAGIATQVKVEEDLAKKGIKKSDLKREEFIKKCYHKEKQYLKI HHHHCCCCCEEEECCCCCCCCEEEEEEHHHHHHCCCCHHCCCHHHHHHHHHHHHHHHEEE KEQDKLGICYDFSKERFTLDQDAQIAVSDFFIKLEKNLIYRGQKAINDIKLQTAISNIEV ECCCCCEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH INKPVEQKMYYLKYFLENSNEFLTVATTRIETISSDVALAINPKDKRYLHLVGKKVVHPL HCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHH TKKLIKIIADSNVGSDFGSGIMKVSAHSILDFEIMEKHNLESKDCIDNYGNLNHEVPEFQ HHHHHHHHHCCCCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCC GQNRFFARDLIAKKLEKEGFLAKIETVISNVGFSQRSDEIVEILKKPQFVKMDELAKSLI CCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHH SHLNSKDKIKFYPKNFEKNLRKFEKIHDTISRQLWGHRIPVYKNDEFKVQIDSPGQGIQD HHCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCC EDVLDTWFSSGISVFAFLGPQNFDLIKSYFPTSLLVTGWDILFFWVARMYFSSLFIMKQK HHHHHHHHHCCCEEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC PFEKVLLHGLIRDEIGRKMSKSLGNGLDPMEIIEKYGSDTLRQALIFNSSPGKDIKFNIE CHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEE KLNTANLNNKINIAKYIADLDTFFAKPDLIDLMENKIYILKRQIVKNIKKYNFSVIGTEI ECCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHHHHCCCEEEEEEC NNFIYGDFSSRYIELIKTRKNGFYARKLLRKVLIILHPFLPFLTDFLMEKIFKMEILEQK CCCEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH MPRIRQFKENQKVENILEIIDNLRTYREKFQISKKIILEYCIINDKFSNAEIDIINKLTF HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHH GKWLENKELVIKTKNFEIAIKVPEELKKEQKGRELKEIQFLKSEILRAEKILTNKGFLEK HHHCCCCEEEEEECCEEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCHHHH APREKIDLERTKLEKLKEKLAFYEKK CCCHHHHHHHHHHHHHHHHHHHHCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: NA