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The map label for this gene is topA

Identifier: 54020038

GI number: 54020038

Start: 110806

End: 112716

Strand: Direct

Name: topA

Synonym: mhp097

Alternate gene names: 54020038

Gene position: 110806-112716 (Clockwise)

Preceding gene: 54020037

Following gene: 54020039

Centisome position: 12.41

GC content: 30.77

Gene sequence:

>1911_bases
GTGGAAAAATTAATTATTGTCGAATCCCCTAATAAAATTAATACAATTTCATCGTATCTTGATTCAAGTTATAAAGTTAC
AGCTTGCGTCGGTCATATCACAAATCTTGCTAAAACTGGACAGTTTGGTTTAGGAATTGATATTAACACTTGAACTCCAT
CTTATGTTCTTGACAAGACCAAGAAAAAAACAATTGATGAGATTAAAAAGTTAGCAAAACAAGCAAAAACTGTAATAATT
GCCACCGATGCCGACCGCGAAGGCGAGGCAATTGGCGCTTCACTTGTCGAACATTTAGCCATTACTGATAAATACCAACG
GATTAAATATAACGAAATTACCAAAACAGCAATACTCGAAGCCTTGGAAAACCCTGGTGAACTTAATCAAAATTTAATAA
ATTCCCAACAAACAAGGCGAATGTTAGATCGAATTATTGGATTTCGGCTAACTGCCTTATTGAAAAATAAAATTAAAAAT
CCACCTAAAAATCCGGCAGCAGGTCGCGTTCAATCATTTGCTCTAAGCCTTATTTTTGACCGTGAAAAAGAAATACGTGA
ATTTATACCTGAAAAATATTTTAATTTAGAAGTTAAAATTAATGACGAAAATGGTAATTTTTTTGATACAATTTATTGTA
ATCAAAAAAATGAGGCAAAGAAAGTCAACTGAATTAATTCAGAAGAAGAACTTTCTGAGATAATTGCTGAAATTAAAGAA
AATCCTTATCTAATTTTTAAAAAATATAGCAAAGCAACCCGGAAGGACCCAAAAGTTATCCCGCTAAAACAGGCGACAAT
TTTTAAGAAAATGTCACAATATTCATCTGCTAGTGTCTCAAATGCCCTTCAAAAACTTTATGAGGGTTATGGCGATTATG
GTTTGATTTCCTATCCGCGTACTGATTCAACAAGGCTAAGTCAAACTTTTATTGAAAAAGGTAAAGAATTTATTATTAAA
AATTTTGGACCTAAATATTTTACTAAAAGTATTAAAGGATTTGCTGGCGATCAAGATGCCCATGAGGCCATTAGACCAAC
AAATTTAGAGCTAAGTCCGGAACTAGCAAGAGAAAAATATCATTTAGGTCAAATTGAATTTCAAATTTATCAACTAATTT
ACCAGACAACAATTCAAGCCTTAATGAGATCTCCGGAACGAAAGATTACTAATTTGACTTTCAAAACACCTATAAAAGAT
CATATTTTTTCTTATAGTTATTCAGAGTTTACTTTTGATGGATATTATAAAGCCACTGAAAAACCAAGTTTAAAATTTAA
ACCTGAATTTTCTGATATAGAAAGTTTAATTAGCAAAAAATTTCAGGTCGATAAAAATTTTGATAATTCGATAAAATCTT
CATTACATCAAACGAAACCGCCAGCCCATTTTCGTGATGGAACTTTAATTGAAAAATTAGATGATGAAAAAGTGGGCCGC
CCCTCAACTTTTGCAATTTCAGTAAAAAAATTAAAGGATCATTTATATGTTGATTCTGAATCAAAATCGCTTGTTTTAAG
TGATTTTGGCAAAATTGTCTATGAAAATTTAAAAATAATTTCGCCAGAAATTATTAATGCAAAATTTACAGCTCAAGTTG
AAGAAGAACTTGATTTAATCGCTCAAGGGAAACAAGATTATAAAAAATATCTTGATTCAATGTGATCAAAAATTGAAAAT
ACTATAGAAAAGGCAGTTGAAGTTGAAAAGGTAATAATGGAAGCCAGAACTACTGGAGAAAAATGCACAGAATGTGATGC
TGATTTGGTTTATCGTTATAATCGGAAAACAAATCAGCGTTTTATTGGCTGTTCTAGTTTCCCAAATTGTCGCTTTATAA
AAGTTGATGAAAATGCTAAAAAAGTTTACCGATTCAGAAAGAGAAATACCAAAATTTTAACAAATAAATAA

Upstream 100 bases:

>100_bases
AAAGTTCGCCGCGCGAAATTATATTATATGAAAAAACGTTCTGGAAAGTCTGCTCGTCTTAAAGAAATTAAACGAAAAGA
ATTAAAAAATTTATAAAATC

Downstream 100 bases:

>100_bases
TATATTTTTAAAAAATATAAGAAAATGTTATGGTTGATACTAATTTAATTGTTGTAGTTGTTTTACTTGTAACTTTAATT
ATCGGTTTTTTTGCTTATAG

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 636; Mature: 636

Protein sequence:

>636_residues
MEKLIIVESPNKINTISSYLDSSYKVTACVGHITNLAKTGQFGLGIDINTWTPSYVLDKTKKKTIDEIKKLAKQAKTVII
ATDADREGEAIGASLVEHLAITDKYQRIKYNEITKTAILEALENPGELNQNLINSQQTRRMLDRIIGFRLTALLKNKIKN
PPKNPAAGRVQSFALSLIFDREKEIREFIPEKYFNLEVKINDENGNFFDTIYCNQKNEAKKVNWINSEEELSEIIAEIKE
NPYLIFKKYSKATRKDPKVIPLKQATIFKKMSQYSSASVSNALQKLYEGYGDYGLISYPRTDSTRLSQTFIEKGKEFIIK
NFGPKYFTKSIKGFAGDQDAHEAIRPTNLELSPELAREKYHLGQIEFQIYQLIYQTTIQALMRSPERKITNLTFKTPIKD
HIFSYSYSEFTFDGYYKATEKPSLKFKPEFSDIESLISKKFQVDKNFDNSIKSSLHQTKPPAHFRDGTLIEKLDDEKVGR
PSTFAISVKKLKDHLYVDSESKSLVLSDFGKIVYENLKIISPEIINAKFTAQVEEELDLIAQGKQDYKKYLDSMWSKIEN
TIEKAVEVEKVIMEARTTGEKCTECDADLVYRYNRKTNQRFIGCSSFPNCRFIKVDENAKKVYRFRKRNTKILTNK

Sequences:

>Translated_636_residues
MEKLIIVESPNKINTISSYLDSSYKVTACVGHITNLAKTGQFGLGIDINT*TPSYVLDKTKKKTIDEIKKLAKQAKTVII
ATDADREGEAIGASLVEHLAITDKYQRIKYNEITKTAILEALENPGELNQNLINSQQTRRMLDRIIGFRLTALLKNKIKN
PPKNPAAGRVQSFALSLIFDREKEIREFIPEKYFNLEVKINDENGNFFDTIYCNQKNEAKKVN*INSEEELSEIIAEIKE
NPYLIFKKYSKATRKDPKVIPLKQATIFKKMSQYSSASVSNALQKLYEGYGDYGLISYPRTDSTRLSQTFIEKGKEFIIK
NFGPKYFTKSIKGFAGDQDAHEAIRPTNLELSPELAREKYHLGQIEFQIYQLIYQTTIQALMRSPERKITNLTFKTPIKD
HIFSYSYSEFTFDGYYKATEKPSLKFKPEFSDIESLISKKFQVDKNFDNSIKSSLHQTKPPAHFRDGTLIEKLDDEKVGR
PSTFAISVKKLKDHLYVDSESKSLVLSDFGKIVYENLKIISPEIINAKFTAQVEEELDLIAQGKQDYKKYLDSM*SKIEN
TIEKAVEVEKVIMEARTTGEKCTECDADLVYRYNRKTNQRFIGCSSFPNCRFIKVDENAKKVYRFRKRNTKILTNK
>Mature_636_residues
MEKLIIVESPNKINTISSYLDSSYKVTACVGHITNLAKTGQFGLGIDINT*TPSYVLDKTKKKTIDEIKKLAKQAKTVII
ATDADREGEAIGASLVEHLAITDKYQRIKYNEITKTAILEALENPGELNQNLINSQQTRRMLDRIIGFRLTALLKNKIKN
PPKNPAAGRVQSFALSLIFDREKEIREFIPEKYFNLEVKINDENGNFFDTIYCNQKNEAKKVN*INSEEELSEIIAEIKE
NPYLIFKKYSKATRKDPKVIPLKQATIFKKMSQYSSASVSNALQKLYEGYGDYGLISYPRTDSTRLSQTFIEKGKEFIIK
NFGPKYFTKSIKGFAGDQDAHEAIRPTNLELSPELAREKYHLGQIEFQIYQLIYQTTIQALMRSPERKITNLTFKTPIKD
HIFSYSYSEFTFDGYYKATEKPSLKFKPEFSDIESLISKKFQVDKNFDNSIKSSLHQTKPPAHFRDGTLIEKLDDEKVGR
PSTFAISVKKLKDHLYVDSESKSLVLSDFGKIVYENLKIISPEIINAKFTAQVEEELDLIAQGKQDYKKYLDSM*SKIEN
TIEKAVEVEKVIMEARTTGEKCTECDADLVYRYNRKTNQRFIGCSSFPNCRFIKVDENAKKVYRFRKRNTKILTNK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=559, Percent_Identity=22.0035778175313, Blast_Score=94, Evalue=4e-19,
Organism=Escherichia coli, GI1787529, Length=647, Percent_Identity=30.757341576507, Blast_Score=271, Evalue=8e-74,
Organism=Escherichia coli, GI1788061, Length=631, Percent_Identity=21.7115689381933, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI32563869, Length=594, Percent_Identity=23.5690235690236, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17555378, Length=576, Percent_Identity=22.9166666666667, Blast_Score=86, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323263, Length=623, Percent_Identity=20.7062600321027, Blast_Score=84, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 72644; Mature: 72644

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLIIVESPNKINTISSYLDSSYKVTACVGHITNLAKTGQFGLGIDINTTPSYVLDKTK
CCCEEEEECCCCHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHH
KKTIDEIKKLAKQAKTVIIATDADREGEAIGASLVEHLAITDKYQRIKYNEITKTAILEA
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LENPGELNQNLINSQQTRRMLDRIIGFRLTALLKNKIKNPPKNPAAGRVQSFALSLIFDR
HCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
EKEIREFIPEKYFNLEVKINDENGNFFDTIYCNQKNEAKKVNINSEEELSEIIAEIKENP
HHHHHHHCCHHHEEEEEEEECCCCCEEEEEEECCCCCCCEECCCCHHHHHHHHHHHCCCC
YLIFKKYSKATRKDPKVIPLKQATIFKKMSQYSSASVSNALQKLYEGYGDYGLISYPRTD
EEEEECHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCC
STRLSQTFIEKGKEFIIKNFGPKYFTKSIKGFAGDQDAHEAIRPTNLELSPELAREKYHL
CHHHHHHHHHCCHHHHHHCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHC
GQIEFQIYQLIYQTTIQALMRSPERKITNLTFKTPIKDHIFSYSYSEFTFDGYYKATEKP
CHHHHHHHHHHHHHHHHHHHHCCCHHHHCCEEECCHHHHHHCCCCCCEEECCEEECCCCC
SLKFKPEFSDIESLISKKFQVDKNFDNSIKSSLHQTKPPAHFRDGTLIEKLDDEKVGRPS
CCEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCC
TFAISVKKLKDHLYVDSESKSLVLSDFGKIVYENLKIISPEIINAKFTAQVEEELDLIAQ
EEEEEHHHHHHHHEECCCCCEEHHHHHHHHHHHHHHEECHHHCCCCHHHHHHHHHHHHHC
GKQDYKKYLDSMSKIENTIEKAVEVEKVIMEARTTGEKCTECDADLVYRYNRKTNQRFIG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHEEECCCCCCEEEE
CSSFPNCRFIKVDENAKKVYRFRKRNTKILTNK
CCCCCCCEEEEECCCHHHHHHHHHCCCEEECCC
>Mature Secondary Structure
MEKLIIVESPNKINTISSYLDSSYKVTACVGHITNLAKTGQFGLGIDINTTPSYVLDKTK
CCCEEEEECCCCHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHH
KKTIDEIKKLAKQAKTVIIATDADREGEAIGASLVEHLAITDKYQRIKYNEITKTAILEA
HHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LENPGELNQNLINSQQTRRMLDRIIGFRLTALLKNKIKNPPKNPAAGRVQSFALSLIFDR
HCCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCC
EKEIREFIPEKYFNLEVKINDENGNFFDTIYCNQKNEAKKVNINSEEELSEIIAEIKENP
HHHHHHHCCHHHEEEEEEEECCCCCEEEEEEECCCCCCCEECCCCHHHHHHHHHHHCCCC
YLIFKKYSKATRKDPKVIPLKQATIFKKMSQYSSASVSNALQKLYEGYGDYGLISYPRTD
EEEEECHHHHCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCC
STRLSQTFIEKGKEFIIKNFGPKYFTKSIKGFAGDQDAHEAIRPTNLELSPELAREKYHL
CHHHHHHHHHCCHHHHHHCCCHHHHHHHHCCCCCCCHHHHCCCCCCCCCCHHHHHHHHHC
GQIEFQIYQLIYQTTIQALMRSPERKITNLTFKTPIKDHIFSYSYSEFTFDGYYKATEKP
CHHHHHHHHHHHHHHHHHHHHCCCHHHHCCEEECCHHHHHHCCCCCCEEECCEEECCCCC
SLKFKPEFSDIESLISKKFQVDKNFDNSIKSSLHQTKPPAHFRDGTLIEKLDDEKVGRPS
CCEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHCCCCCCCCCC
TFAISVKKLKDHLYVDSESKSLVLSDFGKIVYENLKIISPEIINAKFTAQVEEELDLIAQ
EEEEEHHHHHHHHEECCCCCEEHHHHHHHHHHHHHHEECHHHCCCCHHHHHHHHHHHHHC
GKQDYKKYLDSMSKIENTIEKAVEVEKVIMEARTTGEKCTECDADLVYRYNRKTNQRFIG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCHHHHEEECCCCCCEEEE
CSSFPNCRFIKVDENAKKVYRFRKRNTKILTNK
CCCCCCCEEEEECCCHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]