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The map label for this gene is trmD

Identifier: 54020036

GI number: 54020036

Start: 109700

End: 110383

Strand: Direct

Name: trmD

Synonym: mhp095

Alternate gene names: 54020036

Gene position: 109700-110383 (Clockwise)

Preceding gene: 54020035

Following gene: 54020037

Centisome position: 12.29

GC content: 31.14

Gene sequence:

>684_bases
ATGAAAATTAACATATTAACACTTTTTCCCCGTTATTTTGAGGTTTTCTGTAGAGAATCAATAATTGGAAAAGCAATTAA
GCATAAAAAAATAACAATAAATGTTGTTAATTTTCGAGACTTTAGTAAAAATAAACACAAAAAAGTTGATGACTATGTCT
ATGGTGGCGGTCCTGGTTTACTTTTACAAATTCAGCCAGTTGTTGATGCGCTTGAAAAGGTAGGTGGTCTTAAAATTGCT
CTTAGTCCACAAGGACAAAAATTTGATCAAGGCGTTGCGAGAAAGCTTGCAAAAGAAGATGAAATTACGATTTTATGTGG
TCATTATGAGGGTTTTGATCAAAGAATTATCGATAATTTTATTGATTTTGAATTATCACTAGGCGATTTTATTCTAACTG
GAGGCGAAATTGCTGCAATGGCAATAATTGATGCAATTATTCGGTTAAAGCCAGATATAATAAATCCAGAATCACTTAAA
AATGAGACTTTTAATGATTTTTTATTAGATTTTCCGCAATATTCGCGACCAGCAAATTTTCGCGGCCTAGAAGTTCCTAA
CGTTCTAATTTCTGGAAATCATAGAGAGATTGGAGAATGACGTCAGGAACAGCGAGAGCTAATTACTAAAAAAAAACGAC
CAGATTTATGAGAAAAATTTTTAAAAATAAAAAATAAAAAATAA

Upstream 100 bases:

>100_bases
AACTCAAACCGTTAGAAATATTTTCTCTAAAAAGGGTGTTTTTAAAAATTTTCTAGAACAAAAATCATCAAGAGCTTAAT
TTTTTTTCTAAATACAACAG

Downstream 100 bases:

>100_bases
ATTAATTTTCCATTATAATTTTTTAAAACAAATTAAAAAAAGGGAGTTCAAAATGCAAGCAAAATTAATCGAAATTCTTG
AATCAAGCCAAATCCGTTTA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MKINILTLFPRYFEVFCRESIIGKAIKHKKITINVVNFRDFSKNKHKKVDDYVYGGGPGLLLQIQPVVDALEKVGGLKIA
LSPQGQKFDQGVARKLAKEDEITILCGHYEGFDQRIIDNFIDFELSLGDFILTGGEIAAMAIIDAIIRLKPDIINPESLK
NETFNDFLLDFPQYSRPANFRGLEVPNVLISGNHREIGEWRQEQRELITKKKRPDLWEKFLKIKNKK

Sequences:

>Translated_227_residues
MKINILTLFPRYFEVFCRESIIGKAIKHKKITINVVNFRDFSKNKHKKVDDYVYGGGPGLLLQIQPVVDALEKVGGLKIA
LSPQGQKFDQGVARKLAKEDEITILCGHYEGFDQRIIDNFIDFELSLGDFILTGGEIAAMAIIDAIIRLKPDIINPESLK
NETFNDFLLDFPQYSRPANFRGLEVPNVLISGNHREIGE*RQEQRELITKKKRPDL*EKFLKIKNKK
>Mature_227_residues
MKINILTLFPRYFEVFCRESIIGKAIKHKKITINVVNFRDFSKNKHKKVDDYVYGGGPGLLLQIQPVVDALEKVGGLKIA
LSPQGQKFDQGVARKLAKEDEITILCGHYEGFDQRIIDNFIDFELSLGDFILTGGEIAAMAIIDAIIRLKPDIINPESLK
NETFNDFLLDFPQYSRPANFRGLEVPNVLISGNHREIGE*RQEQRELITKKKRPDL*EKFLKIKNKK

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=233, Percent_Identity=39.4849785407725, Blast_Score=182, Evalue=2e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 25678; Mature: 25678

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKINILTLFPRYFEVFCRESIIGKAIKHKKITINVVNFRDFSKNKHKKVDDYVYGGGPGL
CEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCEEECCCCEE
LLQIQPVVDALEKVGGLKIALSPQGQKFDQGVARKLAKEDEITILCGHYEGFDQRIIDNF
EEEEHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHH
IDFELSLGDFILTGGEIAAMAIIDAIIRLKPDIINPESLKNETFNDFLLDFPQYSRPANF
HCCEEECCCEEEECCHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCCCCCCCCCCC
RGLEVPNVLISGNHREIGERQEQRELITKKKRPDLEKFLKIKNKK
CCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC
>Mature Secondary Structure
MKINILTLFPRYFEVFCRESIIGKAIKHKKITINVVNFRDFSKNKHKKVDDYVYGGGPGL
CEEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCCEEECCCCEE
LLQIQPVVDALEKVGGLKIALSPQGQKFDQGVARKLAKEDEITILCGHYEGFDQRIIDNF
EEEEHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHH
IDFELSLGDFILTGGEIAAMAIIDAIIRLKPDIINPESLKNETFNDFLLDFPQYSRPANF
HCCEEECCCEEEECCHHHHHHHHHHHHHCCCCCCCHHHHCCCCHHHHHHCCCCCCCCCCC
RGLEVPNVLISGNHREIGERQEQRELITKKKRPDLEKFLKIKNKK
CCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA