Click here to switch to the map view.

The map label for this gene is rutF [H]

Identifier: 53723286

GI number: 53723286

Start: 3046960

End: 3047433

Strand: Reverse

Name: rutF [H]

Synonym: BPSS2268

Alternate gene names: 53723286

Gene position: 3047433-3046960 (Counterclockwise)

Preceding gene: 53723287

Following gene: 53723285

Centisome position: 96.04

GC content: 71.31

Gene sequence:

>474_bases
ATGCGCCGGCTGACATCGACGGTGTCGATCGTCGCGACGCGCGACGCGTGCGCGCGCTACGGCATGGCGGCGACCGCGGT
GAGCGCGCTCAGCACCGAGCCGCCCGCGATCCTCGTATGCATCAATCGCGCGGCGACGCTGCATGCGCCGCTGATGCGCG
TGCGCCGCTTCTCGCTGAACCTGCTGCACGAGCGTCAGCTCGAGCTGATCGCGCCGTTCAGCGGCAAGCTCGATCACGAC
GCGCGTTTCGCGCACGGCGAATGGTGCGATGCGCACGGCGTGCCGATGCTCGCCGGCGCGCAGGCGACGCTGTGGTGCGC
GGTGGACGGCGATTTCAGCTACGGCAGCCATACGATCGTGATCGGCCGGGTCGATTCGGTGTCGACCGCCGGCCCCGTCG
CGCCGCTGTTGTGGCAGGACGGCGCGCCGTGCGCGGCGCGCGCCCTGGCCCCATGCCTGCACGATGCGCAATGA

Upstream 100 bases:

>100_bases
CGCATCGACGCCGAACGCACGCGCATCGGCATCGAACGCCGCCGCATCGCCCACGCCCGCGCCCGCGCCGGATGCGCTCG
CGCAGCATTTCAAGACCGCG

Downstream 100 bases:

>100_bases
CGCCGCGCGGCCGAGGGAATTCCTGCACAAATGCGAAACGCTCATATAGACTGCAAAGCGAATCATCCGATGGTTCCATG
GCCGCAGCGCGGCGCGCCGA

Product: hypothetical protein

Products: NA

Alternate protein names: FMN reductase; NAD(P)H:flavin oxidoreductase [H]

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRRLTSTVSIVATRDACARYGMAATAVSALSTEPPAILVCINRAATLHAPLMRVRRFSLNLLHERQLELIAPFSGKLDHD
ARFAHGEWCDAHGVPMLAGAQATLWCAVDGDFSYGSHTIVIGRVDSVSTAGPVAPLLWQDGAPCAARALAPCLHDAQ

Sequences:

>Translated_157_residues
MRRLTSTVSIVATRDACARYGMAATAVSALSTEPPAILVCINRAATLHAPLMRVRRFSLNLLHERQLELIAPFSGKLDHD
ARFAHGEWCDAHGVPMLAGAQATLWCAVDGDFSYGSHTIVIGRVDSVSTAGPVAPLLWQDGAPCAARALAPCLHDAQ
>Mature_157_residues
MRRLTSTVSIVATRDACARYGMAATAVSALSTEPPAILVCINRAATLHAPLMRVRRFSLNLLHERQLELIAPFSGKLDHD
ARFAHGEWCDAHGVPMLAGAQATLWCAVDGDFSYGSHTIVIGRVDSVSTAGPVAPLLWQDGAPCAARALAPCLHDAQ

Specific function: Catalyzes the reduction of FMN to FMNH2 which is used to reduce pyrimidine by RutA via the Rut pathway [H]

COG id: COG1853

COG function: function code R; Conserved protein/domain typically associated with flavoprotein oxygenases, DIM6/NTAB family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the non-flavoprotein flavin reductase family. RutF subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081819, Length=141, Percent_Identity=29.0780141843972, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019917
- InterPro:   IPR002563
- InterPro:   IPR012349
- InterPro:   IPR009002 [H]

Pfam domain/function: PF01613 Flavin_Reduct [H]

EC number: =1.5.1.29 [H]

Molecular weight: Translated: 16728; Mature: 16728

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
3.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLTSTVSIVATRDACARYGMAATAVSALSTEPPAILVCINRAATLHAPLMRVRRFSLN
CCCCCCCEEEEEEHHHHHHHCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
LLHERQLELIAPFSGKLDHDARFAHGEWCDAHGVPMLAGAQATLWCAVDGDFSYGSHTIV
HHHHCCEEEECCCCCCCCCCCEECCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCEEEE
IGRVDSVSTAGPVAPLLWQDGAPCAARALAPCLHDAQ
EECCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MRRLTSTVSIVATRDACARYGMAATAVSALSTEPPAILVCINRAATLHAPLMRVRRFSLN
CCCCCCCEEEEEEHHHHHHHCHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHH
LLHERQLELIAPFSGKLDHDARFAHGEWCDAHGVPMLAGAQATLWCAVDGDFSYGSHTIV
HHHHCCEEEECCCCCCCCCCCEECCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCEEEE
IGRVDSVSTAGPVAPLLWQDGAPCAARALAPCLHDAQ
EECCCCCCCCCCCCCEEECCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA