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The map label for this gene is purR [H]

Identifier: 53723103

GI number: 53723103

Start: 2825317

End: 2826375

Strand: Reverse

Name: purR [H]

Synonym: BPSS2087

Alternate gene names: 53723103

Gene position: 2826375-2825317 (Counterclockwise)

Preceding gene: 53723105

Following gene: 53723102

Centisome position: 89.08

GC content: 57.51

Gene sequence:

>1059_bases
GTGAGTCGCATAACCAGCATCCAAGTAGCCCAACTTGCCGGCGTGTCGAGAACAACGGTGTCGTTTGTGTTGAACGGCAT
CACCGGCATGGGAATTAGCGAAGAAACTCGCAATCGTGTGCTCGCGGCGGCCCGGAAGCTCGGTTATGTTCCGAACGCCA
TGGCTCGGTCACTCGCCAGTGGCTCGACAAAGACGATTGCTCTGCTCATGCCTTACGGCGACCACATCCATGTCGATGTC
GATGCCTATCTCCCTCGGTTCCTCGCAGGATTGAGTGCCGCCTGTCATGCAACCGGTTACAAGGTGATACTCGAGCCTGT
TAAGGAATCGCACCGCCCCGGGGCATTCCTTGATCTCGTGAATAGTGGGCGCATTGACGGATTGGTGGTTTTAAATCCTC
GAAAGCTAGACGATGGTCACCTTCATGAGCTCGCTCACAAAGGCTTTCCGCTCGTTGTCTTCGGCTCGAACCTAACCGAG
ACGAAATCGATGTGCGCCATTGGCATCGACAATCGATCTGCGGCGCAACGGGCAACGGAACATCTACTCCATCTCGGCCA
TGAGCGGATTGCTCATATCGGCTTCGCGTCCGATGACTATCAGGTAGTGCGCGATCGACTGGATGGCTTCCGGCTGGCAA
TGGAAACACGTGGCCTCGATGTACGTCCTGAATGGATTGCATTTGCCGATTACAGCGCACAAAGCGGATATGAAGCCATG
CGGAAAATGCTCGCCCAGCCTTCCCGCATGACAGCGCTGTTCGCCGGAAACGACACCGTCGCTTTTGGAGCAATGGCCGC
AATCCGCGATGTCGGATTGCGCGTTCCGGACGACCTGGCGGTCGTCGGTTACGACGACATTCCGCTCGCGGCATTCGCGA
CGCCCCCCCTTACGACGGTTCGCACCGAACCCTTCAAGGAGGGAAATGACGCGGCGGCGACGTTGCTGGGGCTTATCGCC
GGCAACCCGGTCGACGGGCTTCATTCCCGCGGACTTGCGCCACTGATTGTTCGCCAATCCTGCGGCGCAAGCGGTCCAAA
TATCGAGAAACCAAACTAA

Upstream 100 bases:

>100_bases
ACCAATGCGCGCTTCTATACTAAAGCGCGTTAGTATCGCGCAGGATTCATTTAGCTTTCGCATTTGCTCTGGACCTAATT
CGAATCAGGAGCAATCTCCA

Downstream 100 bases:

>100_bases
AAGTAGCCTATACATGATGTGCGTGCAAGAATATTTCCATTCCGACTTATCTTTGAGTATATAGAAATTATTACCGAATG
ATCTGCAGTCCGGTAGTAAC

Product: LacI family regulatory protein

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSRITSIQVAQLAGVSRTTVSFVLNGITGMGISEETRNRVLAAARKLGYVPNAMARSLASGSTKTIALLMPYGDHIHVDV
DAYLPRFLAGLSAACHATGYKVILEPVKESHRPGAFLDLVNSGRIDGLVVLNPRKLDDGHLHELAHKGFPLVVFGSNLTE
TKSMCAIGIDNRSAAQRATEHLLHLGHERIAHIGFASDDYQVVRDRLDGFRLAMETRGLDVRPEWIAFADYSAQSGYEAM
RKMLAQPSRMTALFAGNDTVAFGAMAAIRDVGLRVPDDLAVVGYDDIPLAAFATPPLTTVRTEPFKEGNDAAATLLGLIA
GNPVDGLHSRGLAPLIVRQSCGASGPNIEKPN

Sequences:

>Translated_352_residues
MSRITSIQVAQLAGVSRTTVSFVLNGITGMGISEETRNRVLAAARKLGYVPNAMARSLASGSTKTIALLMPYGDHIHVDV
DAYLPRFLAGLSAACHATGYKVILEPVKESHRPGAFLDLVNSGRIDGLVVLNPRKLDDGHLHELAHKGFPLVVFGSNLTE
TKSMCAIGIDNRSAAQRATEHLLHLGHERIAHIGFASDDYQVVRDRLDGFRLAMETRGLDVRPEWIAFADYSAQSGYEAM
RKMLAQPSRMTALFAGNDTVAFGAMAAIRDVGLRVPDDLAVVGYDDIPLAAFATPPLTTVRTEPFKEGNDAAATLLGLIA
GNPVDGLHSRGLAPLIVRQSCGASGPNIEKPN
>Mature_351_residues
SRITSIQVAQLAGVSRTTVSFVLNGITGMGISEETRNRVLAAARKLGYVPNAMARSLASGSTKTIALLMPYGDHIHVDVD
AYLPRFLAGLSAACHATGYKVILEPVKESHRPGAFLDLVNSGRIDGLVVLNPRKLDDGHLHELAHKGFPLVVFGSNLTET
KSMCAIGIDNRSAAQRATEHLLHLGHERIAHIGFASDDYQVVRDRLDGFRLAMETRGLDVRPEWIAFADYSAQSGYEAMR
KMLAQPSRMTALFAGNDTVAFGAMAAIRDVGLRVPDDLAVVGYDDIPLAAFATPPLTTVRTEPFKEGNDAAATLLGLIAG
NPVDGLHSRGLAPLIVRQSCGASGPNIEKPN

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=338, Percent_Identity=31.9526627218935, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1787948, Length=342, Percent_Identity=34.2105263157895, Blast_Score=156, Evalue=2e-39,
Organism=Escherichia coli, GI1790194, Length=339, Percent_Identity=32.7433628318584, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1789068, Length=313, Percent_Identity=30.6709265175719, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788474, Length=348, Percent_Identity=31.0344827586207, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787580, Length=319, Percent_Identity=28.8401253918495, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI1789202, Length=299, Percent_Identity=30.7692307692308, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=29.8507462686567, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI48994940, Length=325, Percent_Identity=29.2307692307692, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789456, Length=296, Percent_Identity=29.3918918918919, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1787906, Length=306, Percent_Identity=30.3921568627451, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1790689, Length=323, Percent_Identity=26.0061919504644, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1786268, Length=303, Percent_Identity=26.7326732673267, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1790715, Length=328, Percent_Identity=25.3048780487805, Blast_Score=78, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 37701; Mature: 37569

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRITSIQVAQLAGVSRTTVSFVLNGITGMGISEETRNRVLAAARKLGYVPNAMARSLAS
CCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHC
GSTKTIALLMPYGDHIHVDVDAYLPRFLAGLSAACHATGYKVILEPVKESHRPGAFLDLV
CCCCEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEC
NSGRIDGLVVLNPRKLDDGHLHELAHKGFPLVVFGSNLTETKSMCAIGIDNRSAAQRATE
CCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHH
HLLHLGHERIAHIGFASDDYQVVRDRLDGFRLAMETRGLDVRPEWIAFADYSAQSGYEAM
HHHHHCHHHHHHCCCCCCHHHHHHHHHCCEEEEHECCCCCCCCCEEEEECCCCCCHHHHH
RKMLAQPSRMTALFAGNDTVAFGAMAAIRDVGLRVPDDLAVVGYDDIPLAAFATPPLTTV
HHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEECCCCCCCC
RTEPFKEGNDAAATLLGLIAGNPVDGLHSRGLAPLIVRQSCGASGPNIEKPN
CCCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCEEEEHHCCCCCCCCCCCC
>Mature Secondary Structure 
SRITSIQVAQLAGVSRTTVSFVLNGITGMGISEETRNRVLAAARKLGYVPNAMARSLAS
CCCCHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHC
GSTKTIALLMPYGDHIHVDVDAYLPRFLAGLSAACHATGYKVILEPVKESHRPGAFLDLV
CCCCEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEC
NSGRIDGLVVLNPRKLDDGHLHELAHKGFPLVVFGSNLTETKSMCAIGIDNRSAAQRATE
CCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCHHHHHHHHH
HLLHLGHERIAHIGFASDDYQVVRDRLDGFRLAMETRGLDVRPEWIAFADYSAQSGYEAM
HHHHHCHHHHHHCCCCCCHHHHHHHHHCCEEEEHECCCCCCCCCEEEEECCCCCCHHHHH
RKMLAQPSRMTALFAGNDTVAFGAMAAIRDVGLRVPDDLAVVGYDDIPLAAFATPPLTTV
HHHHCCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEECCCCCCCC
RTEPFKEGNDAAATLLGLIAGNPVDGLHSRGLAPLIVRQSCGASGPNIEKPN
CCCCCCCCCHHHHHHHHHHCCCCCCHHHHCCCCCEEEEHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA