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The map label for this gene is pfkB [H]

Identifier: 53722978

GI number: 53722978

Start: 2645187

End: 2646128

Strand: Reverse

Name: pfkB [H]

Synonym: BPSS1957

Alternate gene names: 53722978

Gene position: 2646128-2645187 (Counterclockwise)

Preceding gene: 53722979

Following gene: 53722974

Centisome position: 83.4

GC content: 74.31

Gene sequence:

>942_bases
ATGCCCGAAATCGTCACCCTCACGCCGAATCCCGCCATCGACGTCGCGACGTGCGTCGAGCGAGTCACCGACACCCGCAA
GCTGCGCTGCGGCCCCGCACGACGCGATCCGGGCGGCGGCGGAATCAATGTCGCCCGCGTGCTGACGCGCCTCGGCGCCG
ATTGTTCGGCCGTCTACCTGGCGGGCGGCGGCACCGGCCTCGCGCTGCGCGGCCTGCTCGCCGACGAAGGCGTGCGGGCG
CATGGCATCGACATCGCCGGCGAGACGCGCGAAAACTTTTCGGTGCTCGAAACGTCGACGGGCCGCGAATTCCGCTTCGT
GTTGCCCGGGCCGGCGCTCGCGGCGCACGAATGGCCGCGCTGCGTCGAAGCGCTCGGGCGGCTCGCCGACGCGTCGCGCT
ATCTGGTGATGAGCGGCAGCCTGCCGCCCGGCATGCCGGACGATTGCTACGCCCGGCTCGCGCGGCGCGCGAGCGCGCGC
GGCGTGCGCACGGTCGTCGATACGTCGGGGCCGGCGCTCGCCGCCGCGCTCGATGCGGGCGTCTATCTGGTGAAGCCGAG
CCTCGGTGAGTTGCGCGCGCTGACCGGCCTGCCGCTCGAAGACGACGGCGCGCGCCTTGCCGCCGCGCGCGCGATCGTCG
CCGGCGGGCGCGCGCAGATCGTCGCGCTGACGCTCGGCGACGCGGGCGCGCTCGTCGTGTCGCGCGACGATGCGGTGCGT
CTGCCGGGCGTGAAGGTGGCGGTGCGCAGCGCGATCGGCGCGGGCGACAGCTTCGTCGCGGGGCTCGTCGCCGCGCTCAA
TCGCGGCGCGAACGTCGCCGACGCGGCGCGCCATGCGCTCGCCGCCGCGTCGGCGTCGCTGCTGAGCACGGGCACCGCGC
TGGGCACGAAGGAAGACATCGCGCGGATCTATCGCGAACTGCCGGGGACGATCGACGCGTGA

Upstream 100 bases:

>100_bases
CGGCGCGTGCCCGCCGCGCAGCACCCGCGAGCCGCGCACCCGAGCCAGCCGCTGCGTTGTCTTTCCGTCGCGAATTCCCA
CGTTGAAACCGAACGTCACG

Downstream 100 bases:

>100_bases
CACGTGACGCGCCGGGCGCGCGCGCCGCCGGCGGGGCGCTGCCGGGCGGGCGATCTTGACCGCTGCGCGCGGCAGGCGCG
TTGCGTTCGCCGACGCCGAT

Product: 6-phosphofructokinase

Products: ADP; D-Fructose 1,6-bisphosphate; H+ [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MPEIVTLTPNPAIDVATCVERVTDTRKLRCGPARRDPGGGGINVARVLTRLGADCSAVYLAGGGTGLALRGLLADEGVRA
HGIDIAGETRENFSVLETSTGREFRFVLPGPALAAHEWPRCVEALGRLADASRYLVMSGSLPPGMPDDCYARLARRASAR
GVRTVVDTSGPALAAALDAGVYLVKPSLGELRALTGLPLEDDGARLAAARAIVAGGRAQIVALTLGDAGALVVSRDDAVR
LPGVKVAVRSAIGAGDSFVAGLVAALNRGANVADAARHALAAASASLLSTGTALGTKEDIARIYRELPGTIDA

Sequences:

>Translated_313_residues
MPEIVTLTPNPAIDVATCVERVTDTRKLRCGPARRDPGGGGINVARVLTRLGADCSAVYLAGGGTGLALRGLLADEGVRA
HGIDIAGETRENFSVLETSTGREFRFVLPGPALAAHEWPRCVEALGRLADASRYLVMSGSLPPGMPDDCYARLARRASAR
GVRTVVDTSGPALAAALDAGVYLVKPSLGELRALTGLPLEDDGARLAAARAIVAGGRAQIVALTLGDAGALVVSRDDAVR
LPGVKVAVRSAIGAGDSFVAGLVAALNRGANVADAARHALAAASASLLSTGTALGTKEDIARIYRELPGTIDA
>Mature_312_residues
PEIVTLTPNPAIDVATCVERVTDTRKLRCGPARRDPGGGGINVARVLTRLGADCSAVYLAGGGTGLALRGLLADEGVRAH
GIDIAGETRENFSVLETSTGREFRFVLPGPALAAHEWPRCVEALGRLADASRYLVMSGSLPPGMPDDCYARLARRASARG
VRTVVDTSGPALAAALDAGVYLVKPSLGELRALTGLPLEDDGARLAAARAIVAGGRAQIVALTLGDAGALVVSRDDAVRL
PGVKVAVRSAIGAGDSFVAGLVAALNRGANVADAARHALAAASASLLSTGTALGTKEDIARIYRELPGTIDA

Specific function: Key control step of glycolysis. [C]

COG id: COG1105

COG function: function code G; Fructose-1-phosphate kinase and related fructose-6-phosphate kinase (PfkB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the carbohydrate kinase pfkB family [H]

Homologues:

Organism=Escherichia coli, GI1788017, Length=308, Percent_Identity=38.961038961039, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1788493, Length=273, Percent_Identity=32.2344322344322, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: 496 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011611
- InterPro:   IPR002173
- InterPro:   IPR017583 [H]

Pfam domain/function: PF00294 PfkB [H]

EC number: 2.7.1.11 [C]

Molecular weight: Translated: 31942; Mature: 31811

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00583 PFKB_KINASES_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPEIVTLTPNPAIDVATCVERVTDTRKLRCGPARRDPGGGGINVARVLTRLGADCSAVYL
CCCEEEECCCCCHHHHHHHHHHHCCHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEE
AGGGTGLALRGLLADEGVRAHGIDIAGETRENFSVLETSTGREFRFVLPGPALAAHEWPR
ECCCCCHHHHHHHHCCCCEECCCEECCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHH
CVEALGRLADASRYLVMSGSLPPGMPDDCYARLARRASARGVRTVVDTSGPALAAALDAG
HHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCC
VYLVKPSLGELRALTGLPLEDDGARLAAARAIVAGGRAQIVALTLGDAGALVVSRDDAVR
EEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCEE
LPGVKVAVRSAIGAGDSFVAGLVAALNRGANVADAARHALAAASASLLSTGTALGTKEDI
CCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
ARIYRELPGTIDA
HHHHHHCCCCCCC
>Mature Secondary Structure 
PEIVTLTPNPAIDVATCVERVTDTRKLRCGPARRDPGGGGINVARVLTRLGADCSAVYL
CCEEEECCCCCHHHHHHHHHHHCCHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEE
AGGGTGLALRGLLADEGVRAHGIDIAGETRENFSVLETSTGREFRFVLPGPALAAHEWPR
ECCCCCHHHHHHHHCCCCEECCCEECCCCCCCCEEEEECCCCEEEEEECCCCHHHHHHHH
CVEALGRLADASRYLVMSGSLPPGMPDDCYARLARRASARGVRTVVDTSGPALAAALDAG
HHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHCC
VYLVKPSLGELRALTGLPLEDDGARLAAARAIVAGGRAQIVALTLGDAGALVVSRDDAVR
EEEECCCHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEEEEEEECCCCEEEEECCCCEE
LPGVKVAVRSAIGAGDSFVAGLVAALNRGANVADAARHALAAASASLLSTGTALGTKEDI
CCCHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
ARIYRELPGTIDA
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Co2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; D-Fructose 6-phosphate [C]

Specific reaction: ATP + D-Fructose 6-phosphate --> ADP + D-Fructose 1,6-bisphosphate + H+ [C]

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]