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The map label for this gene is wcaJ [H]

Identifier: 53722853

GI number: 53722853

Start: 2494171

End: 2495586

Strand: Reverse

Name: wcaJ [H]

Synonym: BPSS1834

Alternate gene names: 53722853

Gene position: 2495586-2494171 (Counterclockwise)

Preceding gene: 53722855

Following gene: 53722852

Centisome position: 78.65

GC content: 63.98

Gene sequence:

>1416_bases
GTGCGGCCGGTTTCCGTCTCACGAGGAAGAACCATGTTGAGCGTGCTGGCTAGGATGATCGATATCGCGATGGCAGTGGC
GGGCGCCATGATCGCCGCCATGATCCATCGCGGCGGCTTCGTGTGGCTCGACGATCTGCAGCGCACCGTCGTCGTGTTCG
ACTGCATGCTCGTCGTCGTGTTCTTTCCGGCGTTCGGTATCTATCAGTCGTGGCGCGGCAAGCCGGTGTTCGGGCTCATG
TGGCGCGTGTCGGTGGCGTGGCTCGTCGTCGAGGGCGCGGGCATTCTGATGAGCTTCAGCGTCCACCACGCGGGCGAGCT
GTCGCGCTTGTGGCTCGGCTACTGGGCGATGGCTACGATCACGTTGCTCGTTGCGTCGAAGGCCTGCGTGTACGCGGTGC
TGCGGCAACTGCGGCGCGGCGGCTACAACCACAAGTCGGTCGCGATCGTCGGCCGCGCGCAGGCGAGCGCGACGAAGCGG
CTCATCGCCCACATGCGGGCGCGTCCGGATGCGGGCTTCAATCCGGTCTGCATCTACGACGAGCACGACGACGCCTCGAC
GGGCGAAACCGACGGCATCGCGATCGAGCGCCGCTTCGGCCATCTCGTGCGCCGCGTGCGCAGCCGCGAGATCGGCGAGC
TCTGGCTCGTGCTGCCCCTTTCCGAGGAACCGCAGATTCACCGGATCGTCACCGAGTTCCGAAACGATTTCGTCAACATC
CGGTTCGTGCCGGACGTGCGCAGCCTGTCGTTCTTCAATCAGGAGGTCATCGAGCTGCTCGGCGTGCCCGCGATCAATCT
CGCCGCTTCGCCGATCACCGACGTGCGGATCCTGCCGAAGTTCGTGTTCGATCGGCTGTTCGCGCTCGTCGCGCTCATGA
TGCTCTCGCCGGCGATGCTCGTGATCGCGACACTCGTGAAGCTCACGTCGCCCGGCCCGGTGTTCTTCCGGCAGAAGCGC
AAGGGCATCGACGGCAACGAGTTCGAGATCTACAAGTTCCGCTCGATGAAGGTGCACCAGGAGATGGGGGGAAAGGTCAC
GCAGGCGAGGAAGAACGATTCGCGCGTGACGCCGGTCGGCCGCTTCCTGCGCCGCACGAGCCTCGACGAGCTTCCGCAGT
TCATCAATGTGCTCAAGGGCGAGATGTCGGTGGTCGGCCCGCGCCCGCACGCGCTCGAGCACGACGACATCTACAAGGAT
CTCGTCAAGGGCTACATGTTCCGCTACCGGATCAAGCCGGGCATCACCGGCTGGGCGCAGATCAACGGCTTTCGCGGCGA
GACCGATCGCATCGAGAAGATGATGGGCCGCGTGAAGCTCGATCTGTACTACATGCAGAACTGGTCGTTCTGGCTCGACA
TCAAGATCGTCGCGCTGACGTTGTGGAAAGGCTTCACCGGCAGCAACGCGTATTGA

Upstream 100 bases:

>100_bases
CGATTATTTTTAGAAATACTTGTGCGCTTGCGGGAGGAATTTTCTTCCCGGTTCAATAACGGCATGCAATGTTTAAATCG
GATGCACGTCGACGAGATGT

Downstream 100 bases:

>100_bases
GCGCGGTCATGCGCTTACGACTCACTGGGTAAAGAGGTTCGACAGATGAATCTGACTATCATCGGCAGCGGTTATGTAGG
GCTCGTCACCGGCGCGTGTC

Product: lipopolysaccharide biosynthesis related protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MRPVSVSRGRTMLSVLARMIDIAMAVAGAMIAAMIHRGGFVWLDDLQRTVVVFDCMLVVVFFPAFGIYQSWRGKPVFGLM
WRVSVAWLVVEGAGILMSFSVHHAGELSRLWLGYWAMATITLLVASKACVYAVLRQLRRGGYNHKSVAIVGRAQASATKR
LIAHMRARPDAGFNPVCIYDEHDDASTGETDGIAIERRFGHLVRRVRSREIGELWLVLPLSEEPQIHRIVTEFRNDFVNI
RFVPDVRSLSFFNQEVIELLGVPAINLAASPITDVRILPKFVFDRLFALVALMMLSPAMLVIATLVKLTSPGPVFFRQKR
KGIDGNEFEIYKFRSMKVHQEMGGKVTQARKNDSRVTPVGRFLRRTSLDELPQFINVLKGEMSVVGPRPHALEHDDIYKD
LVKGYMFRYRIKPGITGWAQINGFRGETDRIEKMMGRVKLDLYYMQNWSFWLDIKIVALTLWKGFTGSNAY

Sequences:

>Translated_471_residues
MRPVSVSRGRTMLSVLARMIDIAMAVAGAMIAAMIHRGGFVWLDDLQRTVVVFDCMLVVVFFPAFGIYQSWRGKPVFGLM
WRVSVAWLVVEGAGILMSFSVHHAGELSRLWLGYWAMATITLLVASKACVYAVLRQLRRGGYNHKSVAIVGRAQASATKR
LIAHMRARPDAGFNPVCIYDEHDDASTGETDGIAIERRFGHLVRRVRSREIGELWLVLPLSEEPQIHRIVTEFRNDFVNI
RFVPDVRSLSFFNQEVIELLGVPAINLAASPITDVRILPKFVFDRLFALVALMMLSPAMLVIATLVKLTSPGPVFFRQKR
KGIDGNEFEIYKFRSMKVHQEMGGKVTQARKNDSRVTPVGRFLRRTSLDELPQFINVLKGEMSVVGPRPHALEHDDIYKD
LVKGYMFRYRIKPGITGWAQINGFRGETDRIEKMMGRVKLDLYYMQNWSFWLDIKIVALTLWKGFTGSNAY
>Mature_471_residues
MRPVSVSRGRTMLSVLARMIDIAMAVAGAMIAAMIHRGGFVWLDDLQRTVVVFDCMLVVVFFPAFGIYQSWRGKPVFGLM
WRVSVAWLVVEGAGILMSFSVHHAGELSRLWLGYWAMATITLLVASKACVYAVLRQLRRGGYNHKSVAIVGRAQASATKR
LIAHMRARPDAGFNPVCIYDEHDDASTGETDGIAIERRFGHLVRRVRSREIGELWLVLPLSEEPQIHRIVTEFRNDFVNI
RFVPDVRSLSFFNQEVIELLGVPAINLAASPITDVRILPKFVFDRLFALVALMMLSPAMLVIATLVKLTSPGPVFFRQKR
KGIDGNEFEIYKFRSMKVHQEMGGKVTQARKNDSRVTPVGRFLRRTSLDELPQFINVLKGEMSVVGPRPHALEHDDIYKD
LVKGYMFRYRIKPGITGWAQINGFRGETDRIEKMMGRVKLDLYYMQNWSFWLDIKIVALTLWKGFTGSNAY

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=469, Percent_Identity=39.6588486140725, Blast_Score=318, Evalue=6e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 53488; Mature: 53488

Theoretical pI: Translated: 10.52; Mature: 10.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPVSVSRGRTMLSVLARMIDIAMAVAGAMIAAMIHRGGFVWLDDLQRTVVVFDCMLVVV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHH
FFPAFGIYQSWRGKPVFGLMWRVSVAWLVVEGAGILMSFSVHHAGELSRLWLGYWAMATI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHEEECCCEEEEEEHHCCCHHHHHHHHHHHHHHH
TLLVASKACVYAVLRQLRRGGYNHKSVAIVGRAQASATKRLIAHMRARPDAGFNPVCIYD
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEEE
EHDDASTGETDGIAIERRFGHLVRRVRSREIGELWLVLPLSEEPQIHRIVTEFRNDFVNI
CCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEE
RFVPDVRSLSFFNQEVIELLGVPAINLAASPITDVRILPKFVFDRLFALVALMMLSPAML
EECCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHH
VIATLVKLTSPGPVFFRQKRKGIDGNEFEIYKFRSMKVHQEMGGKVTQARKNDSRVTPVG
HHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEEECHHHHHHHCCCEECCCCCCCCCCHHH
RFLRRTSLDELPQFINVLKGEMSVVGPRPHALEHDDIYKDLVKGYMFRYRIKPGITGWAQ
HHHHHCCHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCE
INGFRGETDRIEKMMGRVKLDLYYMQNWSFWLDIKIVALTLWKGFTGSNAY
ECCCCCCHHHHHHHHHHHEEEEEEEECCCEEEEEEEEEEEHHCCCCCCCCC
>Mature Secondary Structure
MRPVSVSRGRTMLSVLARMIDIAMAVAGAMIAAMIHRGGFVWLDDLQRTVVVFDCMLVVV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHH
FFPAFGIYQSWRGKPVFGLMWRVSVAWLVVEGAGILMSFSVHHAGELSRLWLGYWAMATI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHEEECCCEEEEEEHHCCCHHHHHHHHHHHHHHH
TLLVASKACVYAVLRQLRRGGYNHKSVAIVGRAQASATKRLIAHMRARPDAGFNPVCIYD
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCEEEEE
EHDDASTGETDGIAIERRFGHLVRRVRSREIGELWLVLPLSEEPQIHRIVTEFRNDFVNI
CCCCCCCCCCCCEEHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHCCCEEE
RFVPDVRSLSFFNQEVIELLGVPAINLAASPITDVRILPKFVFDRLFALVALMMLSPAML
EECCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHH
VIATLVKLTSPGPVFFRQKRKGIDGNEFEIYKFRSMKVHQEMGGKVTQARKNDSRVTPVG
HHHHHHHHCCCCCHHHHHHHCCCCCCCEEEEEEECHHHHHHHCCCEECCCCCCCCCCHHH
RFLRRTSLDELPQFINVLKGEMSVVGPRPHALEHDDIYKDLVKGYMFRYRIKPGITGWAQ
HHHHHCCHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHEEEEECCCCCCCCE
INGFRGETDRIEKMMGRVKLDLYYMQNWSFWLDIKIVALTLWKGFTGSNAY
ECCCCCCHHHHHHHHHHHEEEEEEEECCCEEEEEEEEEEEHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]