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The map label for this gene is phaZ1 [H]

Identifier: 53722803

GI number: 53722803

Start: 2442281

End: 2443327

Strand: Reverse

Name: phaZ1 [H]

Synonym: BPSS1784

Alternate gene names: 53722803

Gene position: 2443327-2442281 (Counterclockwise)

Preceding gene: 53722808

Following gene: 53722802

Centisome position: 77.0

GC content: 71.16

Gene sequence:

>1047_bases
ATGACGGCCGACTGGCCGTTCGCCGAAACGATGGCCGCGGCGGCGGCCGCTTCAGTCGCCTCCGTCGCGGCGTCCGCCGA
CACGGTACCGCCCGCCGCGCGCGAATCGCGCGTACGGCCGCGCGCGGCCGCTTGGGCGGGCGGCGAATGGATCCGCGCCG
ATCACCCGTTGCCGCCCGCGTTCGGGCGCTTCGTCAAGCATCTCGAATACGGGCTGTACGTGCCGTCCGGCGCGAAGACG
CTCGGTTTGCCGCTCGTCGTGATGCTGCACGGCTGCAAGCAGGACATGAATCAGTTCTCGCAAGGCACGCGGATGAACTT
GCTCGCGGACCGATACGGCTTCGCCGTGCTCTACCCGGAGCAGTCGCTCAGCGCGCACGCGCACGGCTGCTGGCACTGGT
ACGAGGACACCGTGCACGGCGGGCGCGGCGAAGCGCAGGCGGTCGTCGCGCTCGTCGACGCGCTCGTCGCCGAGCGCGGC
TTCGACGCATCGCGCGTGTATGCGGCCGGCATGTCGGCGGGCGCGGGGCTCGTATCGTTGCTCGCGCTGCATTTCCCGCG
GCGCTTCGCGGCCGTGGCGCTCCATTCGGGGCCGGCGTTCGGCGATGCGCATTCCGGGATCACCGCGATGGACGTGATGC
GCCGCGGCCTGCACCGCGATCCCGCCGCCGTTGTCGACGCGCTCGTCGAGCCGGGCGCGCATCCGGGAATGCCGGCCTTC
ATCGTGCACGGCGACGACGATCGCGTTGTCGTGCCGAAGAACGCGGACGAACTCGCCGTTCAGTTCCTGCGGCTGAACGG
TCTTGCCGACGCCGATGGTGAACCGGCGGGCGTCGAGCGTTTCGAGACGCGCGCAGCCGACGTGCGCACGCTCGACTATC
GCCGCGACGGCGAATCGGTCGTGCGCCTCTGCCGGGTCCACGGCCTCGCGCATGCGTGGGCGGGCGGCGACGACAGTGTG
CCGTTCCACTCGGCCGCCGGCCCGGATGCGAGCGAGCTGATCTGGTCTTTCTTCGCGTCGCGTGAGCGAGCGGCTGTCGC
GTCGTAA

Upstream 100 bases:

>100_bases
GACCAAAAGTCTGACGAAATTCTGGCTGGGCGGTGTGAAACGCATGCTGCACATGCCGAACGCGCGGACGTCGGCGTCCG
CCCTGAAGGCTGCCCAAAAG

Downstream 100 bases:

>100_bases
CGACGCACGCTCGGGAACGGCTGGCATTGATTCAGGGTTTTCCCTATAATACGGGTAAATACCTAAGAGAAAACCCTTGG
ATTGGAGTGACCGATCATGT

Product: depolymerase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 348; Mature: 347

Protein sequence:

>348_residues
MTADWPFAETMAAAAAASVASVAASADTVPPAARESRVRPRAAAWAGGEWIRADHPLPPAFGRFVKHLEYGLYVPSGAKT
LGLPLVVMLHGCKQDMNQFSQGTRMNLLADRYGFAVLYPEQSLSAHAHGCWHWYEDTVHGGRGEAQAVVALVDALVAERG
FDASRVYAAGMSAGAGLVSLLALHFPRRFAAVALHSGPAFGDAHSGITAMDVMRRGLHRDPAAVVDALVEPGAHPGMPAF
IVHGDDDRVVVPKNADELAVQFLRLNGLADADGEPAGVERFETRAADVRTLDYRRDGESVVRLCRVHGLAHAWAGGDDSV
PFHSAAGPDASELIWSFFASRERAAVAS

Sequences:

>Translated_348_residues
MTADWPFAETMAAAAAASVASVAASADTVPPAARESRVRPRAAAWAGGEWIRADHPLPPAFGRFVKHLEYGLYVPSGAKT
LGLPLVVMLHGCKQDMNQFSQGTRMNLLADRYGFAVLYPEQSLSAHAHGCWHWYEDTVHGGRGEAQAVVALVDALVAERG
FDASRVYAAGMSAGAGLVSLLALHFPRRFAAVALHSGPAFGDAHSGITAMDVMRRGLHRDPAAVVDALVEPGAHPGMPAF
IVHGDDDRVVVPKNADELAVQFLRLNGLADADGEPAGVERFETRAADVRTLDYRRDGESVVRLCRVHGLAHAWAGGDDSV
PFHSAAGPDASELIWSFFASRERAAVAS
>Mature_347_residues
TADWPFAETMAAAAAASVASVAASADTVPPAARESRVRPRAAAWAGGEWIRADHPLPPAFGRFVKHLEYGLYVPSGAKTL
GLPLVVMLHGCKQDMNQFSQGTRMNLLADRYGFAVLYPEQSLSAHAHGCWHWYEDTVHGGRGEAQAVVALVDALVAERGF
DASRVYAAGMSAGAGLVSLLALHFPRRFAAVALHSGPAFGDAHSGITAMDVMRRGLHRDPAAVVDALVEPGAHPGMPAFI
VHGDDDRVVVPKNADELAVQFLRLNGLADADGEPAGVERFETRAADVRTLDYRRDGESVVRLCRVHGLAHAWAGGDDSVP
FHSAAGPDASELIWSFFASRERAAVAS

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 37000; Mature: 36869

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADWPFAETMAAAAAASVASVAASADTVPPAARESRVRPRAAAWAGGEWIRADHPLPPA
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHCCCCCEEECCCCCCHH
FGRFVKHLEYGLYVPSGAKTLGLPLVVMLHGCKQDMNQFSQGTRMNLLADRYGFAVLYPE
HHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEECC
QSLSAHAHGCWHWYEDTVHGGRGEAQAVVALVDALVAERGFDASRVYAAGMSAGAGLVSL
HHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHH
LALHFPRRFAAVALHSGPAFGDAHSGITAMDVMRRGLHRDPAAVVDALVEPGAHPGMPAF
HHHHCCHHHHHEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEE
IVHGDDDRVVVPKNADELAVQFLRLNGLADADGEPAGVERFETRAADVRTLDYRRDGESV
EEECCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHEEECCCCCHHHH
VRLCRVHGLAHAWAGGDDSVPFHSAAGPDASELIWSFFASRERAAVAS
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TADWPFAETMAAAAAASVASVAASADTVPPAARESRVRPRAAAWAGGEWIRADHPLPPA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHCCCCCEEECCCCCCHH
FGRFVKHLEYGLYVPSGAKTLGLPLVVMLHGCKQDMNQFSQGTRMNLLADRYGFAVLYPE
HHHHHHHHHHCEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEECC
QSLSAHAHGCWHWYEDTVHGGRGEAQAVVALVDALVAERGFDASRVYAAGMSAGAGLVSL
HHCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHHHHH
LALHFPRRFAAVALHSGPAFGDAHSGITAMDVMRRGLHRDPAAVVDALVEPGAHPGMPAF
HHHHCCHHHHHEEEECCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCEE
IVHGDDDRVVVPKNADELAVQFLRLNGLADADGEPAGVERFETRAADVRTLDYRRDGESV
EEECCCCEEEECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHEEECCCCCHHHH
VRLCRVHGLAHAWAGGDDSVPFHSAAGPDASELIWSFFASRERAAVAS
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]