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The map label for this gene is purF

Identifier: 53722712

GI number: 53722712

Start: 2326228

End: 2327763

Strand: Reverse

Name: purF

Synonym: BPSS1692

Alternate gene names: 53722712

Gene position: 2327763-2326228 (Counterclockwise)

Preceding gene: 53722713

Following gene: 53722711

Centisome position: 73.36

GC content: 65.43

Gene sequence:

>1536_bases
ATGTGCGGCATCGTAGGCGTTATCTCCCAGTCCCCGGTCAACCAGCTCGTCTATGACAGCCTGCTGCTGCTGCAGCATCG
CGGTCAGGACGCGGCGGGCATCGCGACGGCGGACGGCAGCAATTTCCATATGTACAAGGCGAACGGCATGGTGCGCGACG
TGTTCCGCACGCGCAACATGCGCAGCCTGCCCGGCACGGCCGGAATCGGTCAGGTCCGCTACCCGACGGCGGGCTCCGCG
TCGAGCGAAGCCGAGGCGCAACCGTTCTACGTGAACGCGCCGTTCGGCATCATCCTCGCGCACAACGGCAACCTGACGAA
CTGGCAGCAGTTGAAGGACGAAATGTTCCGCATCGACCGCCGCCACATCAACACGAACTCCGACAGCGAAGTGCTGCTGA
ACGTGCTCGCGCACGAACTGCAGCTCTCGACGACGGGCCTCGAGCTCGATCCCGATTCGCTGTTCAAGGCGGTCGCGGGC
GTGCATCGCCGCGCGCACGGCTCGTATGCGATCGTGTCGCTGATCGCCGGCTACGGTCTGCTCGCGTTTCGCGATCCGTT
CGGCATTCGCCCGCTTTGCATCGGCAAGCTCGAAACCGAGCAGGGCACCGAGTGGATGGTGGCGTCGGAATCGGTCGCGG
TGGAAGGCATCGGCTTCGAATTCGTCCGCGACGTGCAGCCGGGCGAGGCGGTCTTCATCGACGTGAACGGCAAGTTCCAT
AGCCGCCAGTGCGCGGACAAGCCGAGCCTGAACCCGTGCATGTTCGAGTACGTGTATCTCGCGCGCCCGGATTCGTGCCT
CGACGGCGTGCCCGTCTACAACGTACGCCTGCGCATGGGCGATTACCTCGCCGAGAAGATCAGGCGCGAGCTGCCGAACG
TGCCGATCGATGTCGTGATGCCGATTCCCGATTCGTCGCGGCCGGCCGCGATGCAGGTGGCGGCGAAGCTCGGCGTCGAG
TATCGCGAGGGCTTCTTCAAGAACCGCTACGTCGGCCGCACGTTCATCATGCCCGGCCAGGCGGTGCGCAAGAAGTCGGT
GCGCCAGAAGCTCAACGCGATGAGCATCGAGTTCAAGGATAAGCACGTGCTGATCGTCGACGATTCGATCGTGCGCGGCA
CCACGTCGCACGAGATCGTGCAGATGGCGCGCGACGCGGGCGCGAAATCGGTGATCTTCGCGTCGGCGGCGCCGCCCGTG
AAGTTCCCGAACGTCTACGGGATCGACATGCCGACGCGCGGCGAACTCGTCGCGCACGGCCGCACCGACGAGGAAGTCGC
GAAGATCATCGGCGCCGATTATCTGATCTACCAGGACGTCGGCGATCTGCGCCGCGCGGTGCGCGACATCAACCCGTCGC
TCGAAGGCTTCGAGGCGTCGTGCTTCGACGGCGACTACATCACCGGCGACGTCACGCCCGAGTATCTGGACGCGATCGAG
CGCGCCCGCCTCGCGCCCGCGTCGCAGGCGGATCGCGATCCGAGCGGCGAAGGCGCCGAGCGCTCGCAGATGAACCTGCA
GCTGTCGGTCGAATGA

Upstream 100 bases:

>100_bases
CTCAAGCCGCTCTTGCCCGACGGGCTCGCTGCGTACATTCACGTCTGATCCGCGCGAGCGCACCGACCGAACCGTTTTTT
GTATCGTTGAAGGACATGCC

Downstream 100 bases:

>100_bases
GCGCCGCGCACGTCGCCAAAAAGGCGTGATACGATGGGGCCTTGCGTCGATTTGATTTCAGCTTGCGGACGCGGGGCAAC
CCGAAACAGCTAAAGCGAAG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 511; Mature: 511

Protein sequence:

>511_residues
MCGIVGVISQSPVNQLVYDSLLLLQHRGQDAAGIATADGSNFHMYKANGMVRDVFRTRNMRSLPGTAGIGQVRYPTAGSA
SSEAEAQPFYVNAPFGIILAHNGNLTNWQQLKDEMFRIDRRHINTNSDSEVLLNVLAHELQLSTTGLELDPDSLFKAVAG
VHRRAHGSYAIVSLIAGYGLLAFRDPFGIRPLCIGKLETEQGTEWMVASESVAVEGIGFEFVRDVQPGEAVFIDVNGKFH
SRQCADKPSLNPCMFEYVYLARPDSCLDGVPVYNVRLRMGDYLAEKIRRELPNVPIDVVMPIPDSSRPAAMQVAAKLGVE
YREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMSIEFKDKHVLIVDDSIVRGTTSHEIVQMARDAGAKSVIFASAAPPV
KFPNVYGIDMPTRGELVAHGRTDEEVAKIIGADYLIYQDVGDLRRAVRDINPSLEGFEASCFDGDYITGDVTPEYLDAIE
RARLAPASQADRDPSGEGAERSQMNLQLSVE

Sequences:

>Translated_511_residues
MCGIVGVISQSPVNQLVYDSLLLLQHRGQDAAGIATADGSNFHMYKANGMVRDVFRTRNMRSLPGTAGIGQVRYPTAGSA
SSEAEAQPFYVNAPFGIILAHNGNLTNWQQLKDEMFRIDRRHINTNSDSEVLLNVLAHELQLSTTGLELDPDSLFKAVAG
VHRRAHGSYAIVSLIAGYGLLAFRDPFGIRPLCIGKLETEQGTEWMVASESVAVEGIGFEFVRDVQPGEAVFIDVNGKFH
SRQCADKPSLNPCMFEYVYLARPDSCLDGVPVYNVRLRMGDYLAEKIRRELPNVPIDVVMPIPDSSRPAAMQVAAKLGVE
YREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMSIEFKDKHVLIVDDSIVRGTTSHEIVQMARDAGAKSVIFASAAPPV
KFPNVYGIDMPTRGELVAHGRTDEEVAKIIGADYLIYQDVGDLRRAVRDINPSLEGFEASCFDGDYITGDVTPEYLDAIE
RARLAPASQADRDPSGEGAERSQMNLQLSVE
>Mature_511_residues
MCGIVGVISQSPVNQLVYDSLLLLQHRGQDAAGIATADGSNFHMYKANGMVRDVFRTRNMRSLPGTAGIGQVRYPTAGSA
SSEAEAQPFYVNAPFGIILAHNGNLTNWQQLKDEMFRIDRRHINTNSDSEVLLNVLAHELQLSTTGLELDPDSLFKAVAG
VHRRAHGSYAIVSLIAGYGLLAFRDPFGIRPLCIGKLETEQGTEWMVASESVAVEGIGFEFVRDVQPGEAVFIDVNGKFH
SRQCADKPSLNPCMFEYVYLARPDSCLDGVPVYNVRLRMGDYLAEKIRRELPNVPIDVVMPIPDSSRPAAMQVAAKLGVE
YREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMSIEFKDKHVLIVDDSIVRGTTSHEIVQMARDAGAKSVIFASAAPPV
KFPNVYGIDMPTRGELVAHGRTDEEVAKIIGADYLIYQDVGDLRRAVRDINPSLEGFEASCFDGDYITGDVTPEYLDAIE
RARLAPASQADRDPSGEGAERSQMNLQLSVE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=478, Percent_Identity=37.8661087866109, Blast_Score=273, Evalue=2e-73,
Organism=Escherichia coli, GI1788651, Length=502, Percent_Identity=57.5697211155379, Blast_Score=585, Evalue=1e-168,
Organism=Escherichia coli, GI1790167, Length=147, Percent_Identity=31.2925170068027, Blast_Score=65, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17554892, Length=496, Percent_Identity=33.8709677419355, Blast_Score=245, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6323958, Length=499, Percent_Identity=50.7014028056112, Blast_Score=462, Evalue=1e-131,
Organism=Drosophila melanogaster, GI28573187, Length=498, Percent_Identity=36.3453815261044, Blast_Score=275, Evalue=5e-74,
Organism=Drosophila melanogaster, GI24659598, Length=502, Percent_Identity=35.8565737051793, Blast_Score=271, Evalue=6e-73,
Organism=Drosophila melanogaster, GI24659604, Length=478, Percent_Identity=36.6108786610879, Blast_Score=267, Evalue=2e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 56232; Mature: 56232

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIVGVISQSPVNQLVYDSLLLLQHRGQDAAGIATADGSNFHMYKANGMVRDVFRTRNM
CCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEECCCHHHHHHHHCCC
RSLPGTAGIGQVRYPTAGSASSEAEAQPFYVNAPFGIILAHNGNLTNWQQLKDEMFRIDR
CCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHHHH
RHINTNSDSEVLLNVLAHELQLSTTGLELDPDSLFKAVAGVHRRAHGSYAIVSLIAGYGL
HHCCCCCHHHHHHHHHHHHHEEECCCCEECHHHHHHHHHHHHHHCCCCHHHHHHHHHCCE
LAFRDPFGIRPLCIGKLETEQGTEWMVASESVAVEGIGFEFVRDVQPGEAVFIDVNGKFH
EEECCCCCCCCEEEEEEECCCCCEEEEECCCEEEECCCHHHHHCCCCCCEEEEECCCCCC
SRQCADKPSLNPCMFEYVYLARPDSCLDGVPVYNVRLRMGDYLAEKIRRELPNVPIDVVM
CCCCCCCCCCCHHHEEEEEEECCCHHHCCCCEEEEEEHHHHHHHHHHHHHCCCCCEEEEE
PIPDSSRPAAMQVAAKLGVEYREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMSIEFKD
ECCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHEEEEECC
KHVLIVDDSIVRGTTSHEIVQMARDAGAKSVIFASAAPPVKFPNVYGIDMPTRGELVAHG
CEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCEEECC
RTDEEVAKIIGADYLIYQDVGDLRRAVRDINPSLEGFEASCFDGDYITGDVTPEYLDAIE
CCHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCCCCEEECCCCCEEECCCCHHHHHHHH
RARLAPASQADRDPSGEGAERSQMNLQLSVE
HHHCCCCCCCCCCCCCCCCCCCEEEEEEEEC
>Mature Secondary Structure
MCGIVGVISQSPVNQLVYDSLLLLQHRGQDAAGIATADGSNFHMYKANGMVRDVFRTRNM
CCCEEECCCCCHHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEEEECCCHHHHHHHHCCC
RSLPGTAGIGQVRYPTAGSASSEAEAQPFYVNAPFGIILAHNGNLTNWQQLKDEMFRIDR
CCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCEEEEEEECCCCCCHHHHHHHHHHHHH
RHINTNSDSEVLLNVLAHELQLSTTGLELDPDSLFKAVAGVHRRAHGSYAIVSLIAGYGL
HHCCCCCHHHHHHHHHHHHHEEECCCCEECHHHHHHHHHHHHHHCCCCHHHHHHHHHCCE
LAFRDPFGIRPLCIGKLETEQGTEWMVASESVAVEGIGFEFVRDVQPGEAVFIDVNGKFH
EEECCCCCCCCEEEEEEECCCCCEEEEECCCEEEECCCHHHHHCCCCCCEEEEECCCCCC
SRQCADKPSLNPCMFEYVYLARPDSCLDGVPVYNVRLRMGDYLAEKIRRELPNVPIDVVM
CCCCCCCCCCCHHHEEEEEEECCCHHHCCCCEEEEEEHHHHHHHHHHHHHCCCCCEEEEE
PIPDSSRPAAMQVAAKLGVEYREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMSIEFKD
ECCCCCCCHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHEEEEECC
KHVLIVDDSIVRGTTSHEIVQMARDAGAKSVIFASAAPPVKFPNVYGIDMPTRGELVAHG
CEEEEECCCHHCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEECCCCCCCCEEECC
RTDEEVAKIIGADYLIYQDVGDLRRAVRDINPSLEGFEASCFDGDYITGDVTPEYLDAIE
CCHHHHHHHHCCCEEEECCHHHHHHHHHHCCCCCCCCEEECCCCCEEECCCCHHHHHHHH
RARLAPASQADRDPSGEGAERSQMNLQLSVE
HHHCCCCCCCCCCCCCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 7704274 [H]