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The map label for this gene is fdoI

Identifier: 53722686

GI number: 53722686

Start: 2287056

End: 2287682

Strand: Reverse

Name: fdoI

Synonym: BPSS1665

Alternate gene names: 53722686

Gene position: 2287682-2287056 (Counterclockwise)

Preceding gene: 53722687

Following gene: 53722685

Centisome position: 72.1

GC content: 64.11

Gene sequence:

>627_bases
ATGAAACATGACGACCCGAACCTGATCGTCCGCTACACGGCGAACGAGCGCTCGAACCACTGGATCACCGCGATCTCGTT
CGTGCTGCTCGCGCTGTCCGGGCTCGCGCTGTTTCATCCTTCGATGTTCTGGATGACGGCGCTGTTCGGCGGCGGGCAGT
GGACGCGGATCCTGCATCCGTTCGTCGGCCTCGTGATGTTCGCGTCGTTCGCGGTGATGGTCGTGCGCTACTGGCGCCAT
AACCTGCTCGACGCGGGCGACCGCCAGTGGCTGCGGCAAATGGACGACGTGCTCGCGAACCGCGAGGACAAGCTGCCGGA
AGTCGGCCGCTATAACGCCGGACAGAAGCTGCTATTCTTCGTGATGGTGGCGTGTCTGCTGTTGCTGCTCGTCTCCGGGA
TCGTGATCTGGCGGCGCTATTTCTCGCTCTACTTCCCGATCGGCGTGATTCGCGCGGCGGCCGTCGTCCATGCGGCAGCG
GCGTTCGTGCTGATCGTCGGCATCGTCGTGCACGTGTACGCGGCGCTGTGGGTGAAGGGCTCGATCGGCGCGATGGTGCG
CGGCACCGTGACCGTCGGCTGGGCCAGAAAGCACCACCCGAAGTGGTTTCGCGAGAGCGTGAAGTAA

Upstream 100 bases:

>100_bases
TCGGCTTCTTCCACTACACGCGCGTCGGCCCGAACGACGTGACGGACGACGAGGAGGCCGCCGCCCGTGACGAAGCGCGC
CGCATCCAGGAGGACGCGAA

Downstream 100 bases:

>100_bases
TATCGCATCGCGCGAAGCCGGCGCGGCGCGCGGGCCCGGGCGGTCCGCGCGCCGCAAGCCGCGCGCCCGTCGGGCCGCCG
GGCCGCCCCTGCGGGCGGCG

Product: formate dehydrogenase, cytochrome b556 (FDO) subunit

Products: CO2; NADH

Alternate protein names: Aerobic formate dehydrogenase cytochrome b556 subunit; FDH-Z subunit gamma; Formate dehydrogenase-O subunit gamma [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MKHDDPNLIVRYTANERSNHWITAISFVLLALSGLALFHPSMFWMTALFGGGQWTRILHPFVGLVMFASFAVMVVRYWRH
NLLDAGDRQWLRQMDDVLANREDKLPEVGRYNAGQKLLFFVMVACLLLLLVSGIVIWRRYFSLYFPIGVIRAAAVVHAAA
AFVLIVGIVVHVYAALWVKGSIGAMVRGTVTVGWARKHHPKWFRESVK

Sequences:

>Translated_208_residues
MKHDDPNLIVRYTANERSNHWITAISFVLLALSGLALFHPSMFWMTALFGGGQWTRILHPFVGLVMFASFAVMVVRYWRH
NLLDAGDRQWLRQMDDVLANREDKLPEVGRYNAGQKLLFFVMVACLLLLLVSGIVIWRRYFSLYFPIGVIRAAAVVHAAA
AFVLIVGIVVHVYAALWVKGSIGAMVRGTVTVGWARKHHPKWFRESVK
>Mature_208_residues
MKHDDPNLIVRYTANERSNHWITAISFVLLALSGLALFHPSMFWMTALFGGGQWTRILHPFVGLVMFASFAVMVVRYWRH
NLLDAGDRQWLRQMDDVLANREDKLPEVGRYNAGQKLLFFVMVACLLLLLVSGIVIWRRYFSLYFPIGVIRAAAVVHAAA
AFVLIVGIVVHVYAALWVKGSIGAMVRGTVTVGWARKHHPKWFRESVK

Specific function: Allows to use formate as major electron donor during aerobic respiration. Subunit gamma is probably the cytochrome b556(FDO) component of the formate dehydrogenase [H]

COG id: COG2864

COG function: function code C; Cytochrome b subunit of formate dehydrogenase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the formate dehydrogenase gamma subunit family [H]

Homologues:

Organism=Escherichia coli, GI1790325, Length=208, Percent_Identity=54.3269230769231, Blast_Score=219, Evalue=9e-59,
Organism=Escherichia coli, GI1787750, Length=205, Percent_Identity=45.3658536585366, Blast_Score=193, Evalue=8e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005797
- InterPro:   IPR016174
- InterPro:   IPR006471 [H]

Pfam domain/function: PF00033 Cytochrom_B_N [H]

EC number: 1.2.1.2

Molecular weight: Translated: 23663; Mature: 23663

Theoretical pI: Translated: 10.83; Mature: 10.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHDDPNLIVRYTANERSNHWITAISFVLLALSGLALFHPSMFWMTALFGGGQWTRILHP
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FVGLVMFASFAVMVVRYWRHNLLDAGDRQWLRQMDDVLANREDKLPEVGRYNAGQKLLFF
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
VMVACLLLLLVSGIVIWRRYFSLYFPIGVIRAAAVVHAAAAFVLIVGIVVHVYAALWVKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SIGAMVRGTVTVGWARKHHPKWFRESVK
CCCHHHHHHEEECHHHHCCCHHHHHCCC
>Mature Secondary Structure
MKHDDPNLIVRYTANERSNHWITAISFVLLALSGLALFHPSMFWMTALFGGGQWTRILHP
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FVGLVMFASFAVMVVRYWRHNLLDAGDRQWLRQMDDVLANREDKLPEVGRYNAGQKLLFF
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHH
VMVACLLLLLVSGIVIWRRYFSLYFPIGVIRAAAVVHAAAAFVLIVGIVVHVYAALWVKG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
SIGAMVRGTVTVGWARKHHPKWFRESVK
CCCHHHHHHEEECHHHHCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD+

Specific reaction: formate + NAD+ = CO2 + NADH

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]