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The map label for this gene is pilQ

Identifier: 53722620

GI number: 53722620

Start: 2171401

End: 2173011

Strand: Reverse

Name: pilQ

Synonym: BPSS1597

Alternate gene names: 53722620

Gene position: 2173011-2171401 (Counterclockwise)

Preceding gene: 53722621

Following gene: 53722619

Centisome position: 68.48

GC content: 71.26

Gene sequence:

>1611_bases
ATGAGCCACGACGCGCAACGACCCGACACGGCCGCCGCGCGCGCGACGCCGAGCGCCGCCGACGCCGCCGCGCCGCCGCG
CGGCCCGCGCGCGGTCAGCGAGGCGGGCGATTTCGCCGCGAGCGTCGAGGAGCGCAAGTTCGTCTGCCTGTTCGACGACG
GGCGGCTGCTGATCGCCGAAGGCCACGAGATGAATCCGTTCGTGCTGTCGTATCGCGCGCGCCTCGACCGGATGGGCCGG
CCGTACCGGCCGACGCCGGCGACGCTGATGCAGGTGCGCGAGGCGTACCGCCAGCACGTGGCGGGCGGCGGCGAGCGGCT
CGACCACACGGTGATGCAGGTGCTCGCGAAGGAGCTGATCGGCCGCGCGTGCCGCGAGCGCGCATCCGACGTGCACATTC
GCGTGCGCCGCTTCAGCACGGAGGTCTACTTCCGGATCCACAACGAACTCATGCGCGTGAACGAGCACACGCGCGAGCAC
GGCGAGCGGCTGCTCGCGACGCTGTATGGCGCGATGACGACCGTCTCGGACAACAGCTACCGGCCGAGCGAGCGGCAGGA
CGCGAGCATCGGCGACCGCGACAAGCTGCCGGACGACCTGTACGGCGTGCGGATCGCGACGACGCCGACGAGCGAGGGCA
GCCTGATGGTGCTGCGGCTGCTGTACAACGATGCGGGCGACGCGACCGATCTCGCGGCGCTCGGCTTCGCGCCCGAGCAC
GTCGCCGCGTTCCGCATGCTGCGCGCGCAGCCGCACGGGATGAACATCATCAGCGGCCCGACGGGCTCCGGCAAGTCGAC
GACGCTGCAGCGCATGCTCGCCGCGCAGATCGACGAATCGCACGGCAGCCTGCACGTGATCACCGTCGAGGATCCGATCG
AATATCCGATCGACGGCGCGGTGCAGACGCCGGTGGCGAACGCGCCGACGGAGGACGCCCGCGCGCTCGCGTTCGCGGCG
GCGATCACCAACGCGATGCGGCTCGATCCGGACACGATCATGATCGGCGAGATCCGCGATCGCGCGTCCGGGCAGACGGC
GCTGCGCGCGTCGATGACGGGCCATCAGGTGTGGACGACCGTGCACGCGAACAGTGCGCTCGCGATCGCCGATCGCCTGA
TCGATCTCGGCCTGCACGCGCGGATGATCACCGATCACACGGTGATCTCCGGGCTCATCAGCCAGCGCCTCGTGAAGCTG
CTGTGCCCGCACTGCAAGGTGCGGCTCCTCGATCATGCGGAGCGGATCGAGCCGGGCCTGCTCGCGCGGCTGCGGCTCGC
GCTCGACACCCGGATGAGCGAGGTCTGCATCACGGGCGACGGCTGCGAGCAGTGCGGCATGGCCGGCACGATCGGCCGCA
CGGTGGTGGCCGAGGTGATCCTGCCCGACGCGCGGCTCTTCGAGTTCCTGCGCGACGGCGACAAGGTCGGCGCGCTCGAG
TACTGCACCCGCACGCTCGGCGCGATGACGCTCGCCGAGCACGCACTGCGCAAGGTCGCGGCGGGGCTCGTCGATCCGCG
CGGCGTCGAGCGCGTGGTCGGCGCGCTCGCGCCGGTGACGGGCGACGCGCGACAGCAGCTGTCGCTCGTCGGATTCAGCT
ATGGCACTTGA

Upstream 100 bases:

>100_bases
CGGGCGCGCCGCCGTCGCCTGCCTATGCGGGCAATCCGCCGCTGCCGGCCGACGCGGCCGCGCCGCTGCCGACGTTGCCC
GCGCGGCCGGCGAGCGGATC

Downstream 100 bases:

>100_bases
GCTGAACCGCCGCTGGGCGAAGCTCTGCCTGAACGCCGACGAGCGGCTGCGCGTCTACCGGAAGATCGCGAAGATGCTCG
GCAACGGGCTGCCGTTGCTG

Product: type IV pilus biosynthesis protein

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MSHDAQRPDTAAARATPSAADAAAPPRGPRAVSEAGDFAASVEERKFVCLFDDGRLLIAEGHEMNPFVLSYRARLDRMGR
PYRPTPATLMQVREAYRQHVAGGGERLDHTVMQVLAKELIGRACRERASDVHIRVRRFSTEVYFRIHNELMRVNEHTREH
GERLLATLYGAMTTVSDNSYRPSERQDASIGDRDKLPDDLYGVRIATTPTSEGSLMVLRLLYNDAGDATDLAALGFAPEH
VAAFRMLRAQPHGMNIISGPTGSGKSTTLQRMLAAQIDESHGSLHVITVEDPIEYPIDGAVQTPVANAPTEDARALAFAA
AITNAMRLDPDTIMIGEIRDRASGQTALRASMTGHQVWTTVHANSALAIADRLIDLGLHARMITDHTVISGLISQRLVKL
LCPHCKVRLLDHAERIEPGLLARLRLALDTRMSEVCITGDGCEQCGMAGTIGRTVVAEVILPDARLFEFLRDGDKVGALE
YCTRTLGAMTLAEHALRKVAAGLVDPRGVERVVGALAPVTGDARQQLSLVGFSYGT

Sequences:

>Translated_536_residues
MSHDAQRPDTAAARATPSAADAAAPPRGPRAVSEAGDFAASVEERKFVCLFDDGRLLIAEGHEMNPFVLSYRARLDRMGR
PYRPTPATLMQVREAYRQHVAGGGERLDHTVMQVLAKELIGRACRERASDVHIRVRRFSTEVYFRIHNELMRVNEHTREH
GERLLATLYGAMTTVSDNSYRPSERQDASIGDRDKLPDDLYGVRIATTPTSEGSLMVLRLLYNDAGDATDLAALGFAPEH
VAAFRMLRAQPHGMNIISGPTGSGKSTTLQRMLAAQIDESHGSLHVITVEDPIEYPIDGAVQTPVANAPTEDARALAFAA
AITNAMRLDPDTIMIGEIRDRASGQTALRASMTGHQVWTTVHANSALAIADRLIDLGLHARMITDHTVISGLISQRLVKL
LCPHCKVRLLDHAERIEPGLLARLRLALDTRMSEVCITGDGCEQCGMAGTIGRTVVAEVILPDARLFEFLRDGDKVGALE
YCTRTLGAMTLAEHALRKVAAGLVDPRGVERVVGALAPVTGDARQQLSLVGFSYGT
>Mature_535_residues
SHDAQRPDTAAARATPSAADAAAPPRGPRAVSEAGDFAASVEERKFVCLFDDGRLLIAEGHEMNPFVLSYRARLDRMGRP
YRPTPATLMQVREAYRQHVAGGGERLDHTVMQVLAKELIGRACRERASDVHIRVRRFSTEVYFRIHNELMRVNEHTREHG
ERLLATLYGAMTTVSDNSYRPSERQDASIGDRDKLPDDLYGVRIATTPTSEGSLMVLRLLYNDAGDATDLAALGFAPEHV
AAFRMLRAQPHGMNIISGPTGSGKSTTLQRMLAAQIDESHGSLHVITVEDPIEYPIDGAVQTPVANAPTEDARALAFAAA
ITNAMRLDPDTIMIGEIRDRASGQTALRASMTGHQVWTTVHANSALAIADRLIDLGLHARMITDHTVISGLISQRLVKLL
CPHCKVRLLDHAERIEPGLLARLRLALDTRMSEVCITGDGCEQCGMAGTIGRTVVAEVILPDARLFEFLRDGDKVGALEY
CTRTLGAMTLAEHALRKVAAGLVDPRGVERVVGALAPVTGDARQQLSLVGFSYGT

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=403, Percent_Identity=31.0173697270471, Blast_Score=161, Evalue=9e-41,
Organism=Escherichia coli, GI1786296, Length=432, Percent_Identity=30.3240740740741, Blast_Score=153, Evalue=3e-38,
Organism=Escherichia coli, GI87082188, Length=163, Percent_Identity=34.9693251533742, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 58297; Mature: 58166

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHDAQRPDTAAARATPSAADAAAPPRGPRAVSEAGDFAASVEERKFVCLFDDGRLLIAE
CCCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCEEEEEC
GHEMNPFVLSYRARLDRMGRPYRPTPATLMQVREAYRQHVAGGGERLDHTVMQVLAKELI
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
GRACRERASDVHIRVRRFSTEVYFRIHNELMRVNEHTREHGERLLATLYGAMTTVSDNSY
HHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RPSERQDASIGDRDKLPDDLYGVRIATTPTSEGSLMVLRLLYNDAGDATDLAALGFAPEH
CCCCCCCCCCCCCCCCCCHHHEEEEEECCCCCCCEEEEEEHHCCCCCHHHHHHHCCCHHH
VAAFRMLRAQPHGMNIISGPTGSGKSTTLQRMLAAQIDESHGSLHVITVEDPIEYPIDGA
HHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
VQTPVANAPTEDARALAFAAAITNAMRLDPDTIMIGEIRDRASGQTALRASMTGHQVWTT
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECHHCCCCCCHHHHHHCCCCEEEEE
VHANSALAIADRLIDLGLHARMITDHTVISGLISQRLVKLLCPHCKVRLLDHAERIEPGL
EECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHH
LARLRLALDTRMSEVCITGDGCEQCGMAGTIGRTVVAEVILPDARLFEFLRDGDKVGALE
HHHHHHHHHHCCHHEEECCCCHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHH
YCTRTLGAMTLAEHALRKVAAGLVDPRGVERVVGALAPVTGDARQQLSLVGFSYGT
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC
>Mature Secondary Structure 
SHDAQRPDTAAARATPSAADAAAPPRGPRAVSEAGDFAASVEERKFVCLFDDGRLLIAE
CCCCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEECCEEEEEC
GHEMNPFVLSYRARLDRMGRPYRPTPATLMQVREAYRQHVAGGGERLDHTVMQVLAKELI
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
GRACRERASDVHIRVRRFSTEVYFRIHNELMRVNEHTREHGERLLATLYGAMTTVSDNSY
HHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
RPSERQDASIGDRDKLPDDLYGVRIATTPTSEGSLMVLRLLYNDAGDATDLAALGFAPEH
CCCCCCCCCCCCCCCCCCHHHEEEEEECCCCCCCEEEEEEHHCCCCCHHHHHHHCCCHHH
VAAFRMLRAQPHGMNIISGPTGSGKSTTLQRMLAAQIDESHGSLHVITVEDPIEYPIDGA
HHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCC
VQTPVANAPTEDARALAFAAAITNAMRLDPDTIMIGEIRDRASGQTALRASMTGHQVWTT
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECHHCCCCCCHHHHHHCCCCEEEEE
VHANSALAIADRLIDLGLHARMITDHTVISGLISQRLVKLLCPHCKVRLLDHAERIEPGL
EECCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCHHH
LARLRLALDTRMSEVCITGDGCEQCGMAGTIGRTVVAEVILPDARLFEFLRDGDKVGALE
HHHHHHHHHHCCHHEEECCCCHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHH
YCTRTLGAMTLAEHALRKVAAGLVDPRGVERVVGALAPVTGDARQQLSLVGFSYGT
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]