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The map label for this gene is gacA [H]

Identifier: 53722543

GI number: 53722543

Start: 2074346

End: 2075017

Strand: Reverse

Name: gacA [H]

Synonym: BPSS1522

Alternate gene names: 53722543

Gene position: 2075017-2074346 (Counterclockwise)

Preceding gene: 53722544

Following gene: 53722542

Centisome position: 65.4

GC content: 68.75

Gene sequence:

>672_bases
ATGGTCGTAGAGAGAAAGTGTGAAAGGGTGCTCGTCGTCGACAGCCATCCGATCGTGCGCGACGGCATCGAGGTCGTGCT
CGGCAAGGCGTATGCGGGAATCGACGTGGACGGCGCGGCCGACATGGCCGAGGCGAGCGACCGGTGCGGTCGCGATCGTC
CGGATCTCGTGCTGTTGGACGTGATGCTGCCGGGCGAGGATCCGCTGCCGGCGATGGGCGAGATCCTGCGCGCGCACCCG
GGCCTGCGGTTCGTCGTCTTCACCGCGCTCGGCTCGGAGGCGCGCGCGGCGGACGCGATCCGCTTCGGCGCCTCCGGCTA
TCTGCTGAAGTGGTGCAGCCCGGACGGCATCGTGCAGGCGATTCGCCGCGTGATGGCGGGGCGCGTTCACATCGATCCGT
CGCTCGACGAGGCGAGGGTGGCGTCGGCGGCCGGCGGCCGCACGCTCGCCTCGCCCGTCGACGAGCTCACGCGGCGCGAG
AAGCAGGTGCTCAAGCTGATCGCCGACGGGATGCGCAATCGCGAAATCGCGTCGCTGCTGAACATCAGCCCGAAGACGGT
CGATTGCCATCGGCAGCGGCTGATGCAGAAGCTCGGCGCGCGCAACGTCGCGACCGTGATCCACTGGGCGTATCGATACG
GATATGCGAACGCGTGCGCGCCGGCGATGTAG

Upstream 100 bases:

>100_bases
AGGGCATGCGCTGGCGCGGTGGGGGCCGCGCTCCGTTCGGGCGATGCGTCGCGCGGGCGGACGCGCAGGACAGCATGCAT
CGGAGCAACAAGAATCGATT

Downstream 100 bases:

>100_bases
CGCGCGGCGGGCGCGGACGCGAACGCGGCCGCCGCGCGGTGCGGCATGTCGCGCGTGCGGCCGTCGCTCGGATTCGTGCC
GCGCGGCGTACGACACGTGA

Product: two-component response regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 223; Mature: 223

Protein sequence:

>223_residues
MVVERKCERVLVVDSHPIVRDGIEVVLGKAYAGIDVDGAADMAEASDRCGRDRPDLVLLDVMLPGEDPLPAMGEILRAHP
GLRFVVFTALGSEARAADAIRFGASGYLLKWCSPDGIVQAIRRVMAGRVHIDPSLDEARVASAAGGRTLASPVDELTRRE
KQVLKLIADGMRNREIASLLNISPKTVDCHRQRLMQKLGARNVATVIHWAYRYGYANACAPAM

Sequences:

>Translated_223_residues
MVVERKCERVLVVDSHPIVRDGIEVVLGKAYAGIDVDGAADMAEASDRCGRDRPDLVLLDVMLPGEDPLPAMGEILRAHP
GLRFVVFTALGSEARAADAIRFGASGYLLKWCSPDGIVQAIRRVMAGRVHIDPSLDEARVASAAGGRTLASPVDELTRRE
KQVLKLIADGMRNREIASLLNISPKTVDCHRQRLMQKLGARNVATVIHWAYRYGYANACAPAM
>Mature_223_residues
MVVERKCERVLVVDSHPIVRDGIEVVLGKAYAGIDVDGAADMAEASDRCGRDRPDLVLLDVMLPGEDPLPAMGEILRAHP
GLRFVVFTALGSEARAADAIRFGASGYLLKWCSPDGIVQAIRRVMAGRVHIDPSLDEARVASAAGGRTLASPVDELTRRE
KQVLKLIADGMRNREIASLLNISPKTVDCHRQRLMQKLGARNVATVIHWAYRYGYANACAPAM

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=211, Percent_Identity=34.1232227488152, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI1786747, Length=210, Percent_Identity=30.4761904761905, Blast_Score=94, Evalue=5e-21,
Organism=Escherichia coli, GI1788521, Length=213, Percent_Identity=32.3943661971831, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790102, Length=197, Percent_Identity=31.9796954314721, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1787473, Length=182, Percent_Identity=32.4175824175824, Blast_Score=80, Evalue=9e-17,
Organism=Escherichia coli, GI1788712, Length=206, Percent_Identity=26.6990291262136, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1788546, Length=208, Percent_Identity=26.4423076923077, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 24253; Mature: 24253

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVERKCERVLVVDSHPIVRDGIEVVLGKAYAGIDVDGAADMAEASDRCGRDRPDLVLLD
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEE
VMLPGEDPLPAMGEILRAHPGLRFVVFTALGSEARAADAIRFGASGYLLKWCSPDGIVQA
EEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCCCCEEEECCCHHHHHH
IRRVMAGRVHIDPSLDEARVASAAGGRTLASPVDELTRREKQVLKLIADGMRNREIASLL
HHHHHHCCEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
NISPKTVDCHRQRLMQKLGARNVATVIHWAYRYGYANACAPAM
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MVVERKCERVLVVDSHPIVRDGIEVVLGKAYAGIDVDGAADMAEASDRCGRDRPDLVLLD
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEE
VMLPGEDPLPAMGEILRAHPGLRFVVFTALGSEARAADAIRFGASGYLLKWCSPDGIVQA
EEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCCCCEEEECCCHHHHHH
IRRVMAGRVHIDPSLDEARVASAAGGRTLASPVDELTRREKQVLKLIADGMRNREIASLL
HHHHHHCCEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
NISPKTVDCHRQRLMQKLGARNVATVIHWAYRYGYANACAPAM
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]