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The map label for this gene is pksI [H]

Identifier: 53722021

GI number: 53722021

Start: 1320465

End: 1321211

Strand: Reverse

Name: pksI [H]

Synonym: BPSS1000

Alternate gene names: 53722021

Gene position: 1321211-1320465 (Counterclockwise)

Preceding gene: 53722022

Following gene: 53722020

Centisome position: 41.64

GC content: 65.46

Gene sequence:

>747_bases
GTGGCGGACTGCGTAGTCCAGGTCGTCATGCAGGATCGCGAGAACAAGAACACGTTCTCCGACGCGCTGATCCGGGGGCT
CGGCGAGGCATTCGACGCGATCCGCCAGAACGGCCGGTGCAAGGCCGCGATCCTCACCGGCTACGGCAACTATTTCGCGA
GCGGCGGCACGCAGGAAAGCCTCATGTACCTGTCCGAGGGCAAGGGCACGTTCGCGGAGATCGCCGCGAGCGCGAGCCGC
AACGGCGCGAACCTGTACAGCCTCGCGCTGAACTGCCCCGTCCCCGTCATTGCGGCGATGCAGGGGCACGGCATCGGCGG
CGGCTTCGTGATGGGCCTGTTCGCGGACTTCGTCGTGCTCGGCAAGGAATGCCTGTACACCACCAATTTCATGAAGTACG
GCTTCACGCCGGGCATGGGCGCGACGCTCGTGCTGCCGCACAAGCTCGGCCTGCCGCTCGCGCAGAACATGCTGATGACC
GCCCGTTCGTTCTACGGCGACGAGCTGCAAAAGCTCGGCGTGCCGTTTCCGGTGGTGCCGCGCGCGGAAGTGGCCGCGTG
TGCGCTCGAGATCGCGAAGAATCTCGCCGAAAAGCCGCGGCTGTCGCTCGTCACGCTGAAGGATCATCTGGTGGCCGAGC
TGCGCTCGAGACTGCCGGCCGTGATCGAGCAGGAGCTCGCGATGCATGAAAAGACCTTCGGCCAGCCCGAGGTCAAGGAG
CGCATCCGCTCGCTCTTCGGACAATAA

Upstream 100 bases:

>100_bases
GGAGAACCGCCGGAAGATCGCGCGGTACGTCGACACCGGAAAATTTCCCTGGGAGGCGGACTAAGTGAGCGCGCATGAAC
GAGTCGTCGAAGTCCGCGAG

Downstream 100 bases:

>100_bases
GTCATCCAACCCGATCGCCGCCCGTCGGCGACACCCGTCAAGGAAGCGCTGCCATGGACCTGCAGCAGGTATTCGACATT
ATCGTTCGGCATACGAAAGA

Product: polyketide biosynthesis enoyl-CoA hydratase

Products: L-Carnitine.; L-carnitinyl-CoA [C]

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MADCVVQVVMQDRENKNTFSDALIRGLGEAFDAIRQNGRCKAAILTGYGNYFASGGTQESLMYLSEGKGTFAEIAASASR
NGANLYSLALNCPVPVIAAMQGHGIGGGFVMGLFADFVVLGKECLYTTNFMKYGFTPGMGATLVLPHKLGLPLAQNMLMT
ARSFYGDELQKLGVPFPVVPRAEVAACALEIAKNLAEKPRLSLVTLKDHLVAELRSRLPAVIEQELAMHEKTFGQPEVKE
RIRSLFGQ

Sequences:

>Translated_248_residues
MADCVVQVVMQDRENKNTFSDALIRGLGEAFDAIRQNGRCKAAILTGYGNYFASGGTQESLMYLSEGKGTFAEIAASASR
NGANLYSLALNCPVPVIAAMQGHGIGGGFVMGLFADFVVLGKECLYTTNFMKYGFTPGMGATLVLPHKLGLPLAQNMLMT
ARSFYGDELQKLGVPFPVVPRAEVAACALEIAKNLAEKPRLSLVTLKDHLVAELRSRLPAVIEQELAMHEKTFGQPEVKE
RIRSLFGQ
>Mature_247_residues
ADCVVQVVMQDRENKNTFSDALIRGLGEAFDAIRQNGRCKAAILTGYGNYFASGGTQESLMYLSEGKGTFAEIAASASRN
GANLYSLALNCPVPVIAAMQGHGIGGGFVMGLFADFVVLGKECLYTTNFMKYGFTPGMGATLVLPHKLGLPLAQNMLMTA
RSFYGDELQKLGVPFPVVPRAEVAACALEIAKNLAEKPRLSLVTLKDHLVAELRSRLPAVIEQELAMHEKTFGQPEVKER
IRSLFGQ

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. May have a role in the decarboxylation of the (S)-3-methylglutaryl group attached to pksL [H]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=224, Percent_Identity=23.2142857142857, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI221142681, Length=200, Percent_Identity=30, Blast_Score=84, Evalue=7e-18,
Organism=Escherichia coli, GI1787659, Length=210, Percent_Identity=25.7142857142857, Blast_Score=68, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24653139, Length=184, Percent_Identity=28.804347826087, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 26796; Mature: 26664

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADCVVQVVMQDRENKNTFSDALIRGLGEAFDAIRQNGRCKAAILTGYGNYFASGGTQES
CHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCCCCHHH
LMYLSEGKGTFAEIAASASRNGANLYSLALNCPVPVIAAMQGHGIGGGFVMGLFADFVVL
HHHHHCCCCHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHH
GKECLYTTNFMKYGFTPGMGATLVLPHKLGLPLAQNMLMTARSFYGDELQKLGVPFPVVP
HHHHHHHHHHHHHCCCCCCCCEEEECHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RAEVAACALEIAKNLAEKPRLSLVTLKDHLVAELRSRLPAVIEQELAMHEKTFGQPEVKE
CHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RIRSLFGQ
HHHHHHCC
>Mature Secondary Structure 
ADCVVQVVMQDRENKNTFSDALIRGLGEAFDAIRQNGRCKAAILTGYGNYFASGGTQES
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHCCCCCHHH
LMYLSEGKGTFAEIAASASRNGANLYSLALNCPVPVIAAMQGHGIGGGFVMGLFADFVVL
HHHHHCCCCHHHHHHHHCCCCCCEEEEEEECCCCHHHHHHCCCCCCHHHHHHHHHHHHHH
GKECLYTTNFMKYGFTPGMGATLVLPHKLGLPLAQNMLMTARSFYGDELQKLGVPFPVVP
HHHHHHHHHHHHHCCCCCCCCEEEECHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RAEVAACALEIAKNLAEKPRLSLVTLKDHLVAELRSRLPAVIEQELAMHEKTFGQPEVKE
CHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
RIRSLFGQ
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Carnitine; D-carnitinyl-CoA [C]

Specific reaction: D-Carnitine = L-Carnitine. D-carnitinyl-CoA = L-carnitinyl-CoA [C]

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]