Click here to switch to the map view.

The map label for this gene is pat [H]

Identifier: 53721954

GI number: 53721954

Start: 1226079

End: 1226645

Strand: Reverse

Name: pat [H]

Synonym: BPSS0930

Alternate gene names: 53721954

Gene position: 1226645-1226079 (Counterclockwise)

Preceding gene: 53721956

Following gene: 53721953

Centisome position: 38.66

GC content: 70.72

Gene sequence:

>567_bases
ATGAAAGCCACCGACGCCCCCACCTCTTCCGCCATCGTGCGCGACGCCACCGAACACGATCTCGAAGCGATCCAGGCGAT
CTATGCGCATCACGTGCTGACGGGCGTCGCGTCGTTCGAGGAAACGCCGCCGTCGGTCGACGATCTGCGCGCGCGGCGCG
AAGCGGTGCGGCGGCACGGCCTGCCGTATCTCGTCGCCGAGCTGGACGGCGTCGTCGCGGGCTACGCGTATGCGACGCCG
TATCGGCCGCGCAGTGCATACCGCTATGCGATCGAAGATTCGATCTACGTGAACGAGGCGTGCCGCGGCCGCGGCATCGG
CCGCGTGCTGCTCGCCGCGCTGATCGCGCGCTGCGAGGCGGGCCCGTGGCGGCAGATGATCGCGGTGATCGCCGACGGCG
GGCGCGGCGGCTCGACATCGCTGCATCGCGCGCTCGGCTTCGAGCCCATCGGCACGCTGCGCGGCGTGGGCTTCAAGCAC
GGCCGCTGGATCGACACCGCGCTGATGCAACGCGTGCTCGGAGACGGCGCACAGACGCCCCCCTCGAACGCCGACGCCTC
GCACTGA

Upstream 100 bases:

>100_bases
CGCCCGCCGCGCCCACCGTTCGACGCCGCCGCGCTTGGCGGACCTCGACGAATGCGCGCATAGTAGCGATTTCGTCATTC
CTGCCCTGTTTGTTTTCGAG

Downstream 100 bases:

>100_bases
CACGCGCGGTCGCGCTAAAATCGCCGCATGCCGAAATCGAACTCAACCACTCCCGCCGACGTCGCCCCGGATGCATCGAT
GTCGAACGAATATACGGTCG

Product: phosphinothricin N-acetyltransferase

Products: NA

Alternate protein names: PPT N-acetyltransferase; Phosphinothricin-resistance protein [H]

Number of amino acids: Translated: 188; Mature: 188

Protein sequence:

>188_residues
MKATDAPTSSAIVRDATEHDLEAIQAIYAHHVLTGVASFEETPPSVDDLRARREAVRRHGLPYLVAELDGVVAGYAYATP
YRPRSAYRYAIEDSIYVNEACRGRGIGRVLLAALIARCEAGPWRQMIAVIADGGRGGSTSLHRALGFEPIGTLRGVGFKH
GRWIDTALMQRVLGDGAQTPPSNADASH

Sequences:

>Translated_188_residues
MKATDAPTSSAIVRDATEHDLEAIQAIYAHHVLTGVASFEETPPSVDDLRARREAVRRHGLPYLVAELDGVVAGYAYATP
YRPRSAYRYAIEDSIYVNEACRGRGIGRVLLAALIARCEAGPWRQMIAVIADGGRGGSTSLHRALGFEPIGTLRGVGFKH
GRWIDTALMQRVLGDGAQTPPSNADASH
>Mature_188_residues
MKATDAPTSSAIVRDATEHDLEAIQAIYAHHVLTGVASFEETPPSVDDLRARREAVRRHGLPYLVAELDGVVAGYAYATP
YRPRSAYRYAIEDSIYVNEACRGRGIGRVLLAALIARCEAGPWRQMIAVIADGGRGGSTSLHRALGFEPIGTLRGVGFKH
GRWIDTALMQRVLGDGAQTPPSNADASH

Specific function: This enzyme is an effector of phosphinothricin tripeptide (PTT or bialaphos) resistance. Inactivates PTT by transfer of an acetyl group [H]

COG id: COG1247

COG function: function code M; Sortase and related acyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 N-acetyltransferase domain [H]

Homologues:

Organism=Escherichia coli, GI1787719, Length=163, Percent_Identity=36.1963190184049, Blast_Score=92, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000182
- InterPro:   IPR016181 [H]

Pfam domain/function: PF00583 Acetyltransf_1 [H]

EC number: =2.3.1.183 [H]

Molecular weight: Translated: 20264; Mature: 20264

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKATDAPTSSAIVRDATEHDLEAIQAIYAHHVLTGVASFEETPPSVDDLRARREAVRRHG
CCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
LPYLVAELDGVVAGYAYATPYRPRSAYRYAIEDSIYVNEACRGRGIGRVLLAALIARCEA
CCEEHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEECHHHCCCCHHHHHHHHHHHHCCC
GPWRQMIAVIADGGRGGSTSLHRALGFEPIGTLRGVGFKHGRWIDTALMQRVLGDGAQTP
CHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
PSNADASH
CCCCCCCC
>Mature Secondary Structure
MKATDAPTSSAIVRDATEHDLEAIQAIYAHHVLTGVASFEETPPSVDDLRARREAVRRHG
CCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
LPYLVAELDGVVAGYAYATPYRPRSAYRYAIEDSIYVNEACRGRGIGRVLLAALIARCEA
CCEEHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCEEECHHHCCCCHHHHHHHHHHHHCCC
GPWRQMIAVIADGGRGGSTSLHRALGFEPIGTLRGVGFKHGRWIDTALMQRVLGDGAQTP
CHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCC
PSNADASH
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA