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The map label for this gene is tfdA [H]

Identifier: 53721644

GI number: 53721644

Start: 830360

End: 831238

Strand: Reverse

Name: tfdA [H]

Synonym: BPSS0608

Alternate gene names: 53721644

Gene position: 831238-830360 (Counterclockwise)

Preceding gene: 53721645

Following gene: 53721643

Centisome position: 26.2

GC content: 66.21

Gene sequence:

>879_bases
ATGAGCGAGACGATCACGCGCAGGAAAGTGGCATACAAGACGCTATCCAATGCGCTCGGCACTGAGGTGTACGGCTTCGA
TTCGCCGTTTCCGCACGATGCCGAGGCCGTGCGCGCGCTGATCGAGGCATGGCATGCGGGCGGCATCTGCCGGCTTCGGC
GCCAGCGGCTCGACATGGCGGAGTTCGTCGAGTTCAGCCGGATCTTCGGCAGGCCCGAGCGCGCGCTGAACCAGGAGCGC
AAGCTGACCTCGCGCGAAGACCTGCCCGAACTGATGATCGTGTCGAACATCCTGGAAAACGGCGTGTCGATCGGGCATCT
CGGCTCGAAGGAAGCGTACTGGCACACCGACATGTGCTACACCGACGTGCCGCCGATCGCGAGCATTCTCTATGCGATCG
AGGTGCCCGCGCGCGGCGGCAACACCGAGTTCATGAACATGTACCGCGTGCACGACGCGCTGCCCGCCTCGCTGCGCCGG
CAGATCGCCGGCCTGTCGATCAAGCACGACCGCAGCTACACGGCCGTCGGCGAGCTGCGCTACGGGTTCGATTCGGTCGT
CGACGTGACGACCTGCCCCGGCTCGATTCATCCGATCGTGCGCGTCCATCCGGTCACGCAGCGGCCGTATCTTTATCTCG
GCCGCCGGCTGAACGCGTATGTCGTCGGCCTGCCCGTCGGCGAATCGGAGGCGCTCCTCGACGAGCTGTGGCGCTATACG
CGGCTCGACGGCGTGACGTGGACGCAGCGCTGGGAAGTCGGCGACATCATGATCTGGGACAACCGCTGCACGATGCACCG
GCGCGATGCGTTCGACGCGAACGCGCGGCGCCTGATGTGGCGCACGCAGATCCAGGCCGATCCGGCGCGGCCGCTGTAG

Upstream 100 bases:

>100_bases
TGCCGCCCCGGCTATCGCAGCGATCTCGGCCGGCCGCGCACGTCGCCCGCCGAGAACAAACGCCATTTCATGGCCTTCCT
ATCCCGCAACGGAGCGACAC

Downstream 100 bases:

>100_bases
CGGCCCGCGCATCCGATACGAACGACAGCGAGGCTCAATTGCTCAATCACCGCTCCGCCGCTTTCGCGCGCGTTGCCCGC
GCGCTCGACGCCCGCGGCAA

Product: TauD/TfdA family dioxygenase

Products: Aminoacetaldehyde; CO2; H+; Sulfite; Succinate [C]

Alternate protein names: 2,4-D dioxygenase [H]

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MSETITRRKVAYKTLSNALGTEVYGFDSPFPHDAEAVRALIEAWHAGGICRLRRQRLDMAEFVEFSRIFGRPERALNQER
KLTSREDLPELMIVSNILENGVSIGHLGSKEAYWHTDMCYTDVPPIASILYAIEVPARGGNTEFMNMYRVHDALPASLRR
QIAGLSIKHDRSYTAVGELRYGFDSVVDVTTCPGSIHPIVRVHPVTQRPYLYLGRRLNAYVVGLPVGESEALLDELWRYT
RLDGVTWTQRWEVGDIMIWDNRCTMHRRDAFDANARRLMWRTQIQADPARPL

Sequences:

>Translated_292_residues
MSETITRRKVAYKTLSNALGTEVYGFDSPFPHDAEAVRALIEAWHAGGICRLRRQRLDMAEFVEFSRIFGRPERALNQER
KLTSREDLPELMIVSNILENGVSIGHLGSKEAYWHTDMCYTDVPPIASILYAIEVPARGGNTEFMNMYRVHDALPASLRR
QIAGLSIKHDRSYTAVGELRYGFDSVVDVTTCPGSIHPIVRVHPVTQRPYLYLGRRLNAYVVGLPVGESEALLDELWRYT
RLDGVTWTQRWEVGDIMIWDNRCTMHRRDAFDANARRLMWRTQIQADPARPL
>Mature_291_residues
SETITRRKVAYKTLSNALGTEVYGFDSPFPHDAEAVRALIEAWHAGGICRLRRQRLDMAEFVEFSRIFGRPERALNQERK
LTSREDLPELMIVSNILENGVSIGHLGSKEAYWHTDMCYTDVPPIASILYAIEVPARGGNTEFMNMYRVHDALPASLRRQ
IAGLSIKHDRSYTAVGELRYGFDSVVDVTTCPGSIHPIVRVHPVTQRPYLYLGRRLNAYVVGLPVGESEALLDELWRYTR
LDGVTWTQRWEVGDIMIWDNRCTMHRRDAFDANARRLMWRTQIQADPARPL

Specific function: Involved in degradation of the herbicide 2,4- dichlorophenoxyacetic acid (2,4-D). Is also able to degrade 2- methyl-4-chlorophenoxyacetic acid and 3-chlorobenzoic acid [H]

COG id: COG2175

COG function: function code Q; Probable taurine catabolism dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tfdA dioxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786565, Length=184, Percent_Identity=32.0652173913043, Blast_Score=99, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6322971, Length=279, Percent_Identity=27.9569892473118, Blast_Score=84, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003819 [H]

Pfam domain/function: PF02668 TauD [H]

EC number: 1.14.11.17 [C]

Molecular weight: Translated: 33477; Mature: 33345

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETITRRKVAYKTLSNALGTEVYGFDSPFPHDAEAVRALIEAWHAGGICRLRRQRLDMA
CCCCHHHHHHHHHHHHHHHCCEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHH
EFVEFSRIFGRPERALNQERKLTSREDLPELMIVSNILENGVSIGHLGSKEAYWHTDMCY
HHHHHHHHHCCCHHHHHHHHHCCCHHCCHHHHHHHHHHHCCCEECCCCCCCCEEECCCCC
TDVPPIASILYAIEVPARGGNTEFMNMYRVHDALPASLRRQIAGLSIKHDRSYTAVGELR
CCCCHHHHHHHHEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCEEECCCCEEEHHHHH
YGFDSVVDVTTCPGSIHPIVRVHPVTQRPYLYLGRRLNAYVVGLPVGESEALLDELWRYT
CCCHHEEEEECCCCCCCCEEEEECCCCCCHHHHCCCCCEEEEEECCCCHHHHHHHHHHHH
RLDGVTWTQRWEVGDIMIWDNRCTMHRRDAFDANARRLMWRTQIQADPARPL
HCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SETITRRKVAYKTLSNALGTEVYGFDSPFPHDAEAVRALIEAWHAGGICRLRRQRLDMA
CCCHHHHHHHHHHHHHHHCCEECCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCHH
EFVEFSRIFGRPERALNQERKLTSREDLPELMIVSNILENGVSIGHLGSKEAYWHTDMCY
HHHHHHHHHCCCHHHHHHHHHCCCHHCCHHHHHHHHHHHCCCEECCCCCCCCEEECCCCC
TDVPPIASILYAIEVPARGGNTEFMNMYRVHDALPASLRRQIAGLSIKHDRSYTAVGELR
CCCCHHHHHHHHEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHCCEEECCCCEEEHHHHH
YGFDSVVDVTTCPGSIHPIVRVHPVTQRPYLYLGRRLNAYVVGLPVGESEALLDELWRYT
CCCHHEEEEECCCCCCCCEEEEECCCCCCHHHHCCCCCEEEEEECCCCHHHHHHHHHHHH
RLDGVTWTQRWEVGDIMIWDNRCTMHRRDAFDANARRLMWRTQIQADPARPL
HCCCCCEEEEECCCCEEEECCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Ascorbate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Oxoglutarate; O2; Taurine [C]

Specific reaction: 2-Oxoglutarate + O2 + Taurine --> Aminoacetaldehyde + CO2 + H+ + Sulfite + Succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036764; 10777523; 11457355; 11955067 [H]