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The map label for this gene is pchD

Identifier: 53721620

GI number: 53721620

Start: 792554

End: 794197

Strand: Reverse

Name: pchD

Synonym: BPSS0584

Alternate gene names: 53721620

Gene position: 794197-792554 (Counterclockwise)

Preceding gene: 53721631

Following gene: 53721619

Centisome position: 25.03

GC content: 72.32

Gene sequence:

>1644_bases
TTGCCCGCCGCCGCGCGCAACGCCGCGTCGAGCGACGCGCCCGACTGGCCCGACGACTTCGCGCGCCGCTACCGCGCCGC
CGGCTACTGGCGAGACGAGACCTTCTACGGCGCGCTCGCCGATCGCGCGCGGCGCAGCCCCGCCGCGACCGCGGTCGTCG
ACGGCGAGCGCCGCGTCAGCTACCGCGCGCTGCTCGAGCGCATCCGGCGTCTCGCCGGCGGCTTACGCCGCCTGGGCCTC
GCGCGCGGCGACACGGCCGTCGTGCATCTGCCGAACGGCGTGCGCTTCATCGAAGCGTGCTTCGCGCTGTTCCAGCTCGG
CGTGCGCCCGGTGCTCGCGCTGCCCGCGCATCGGCAACACGAGATCGGCGCGTTCTGCCGCTTCACGAATGCCCGCGCAT
ATCTCGGCGCGGCCCGGCTCGGCGAGTTCGACTCCCACCCGCTCGCTCACGCGCTGCGGGCGAGCTGCCCGGCGCTCGCG
CACATCGTCGTCGCGGGCGACGATCATGCCTTCTTGCATTTCGATTCGCTGTACGACGCGCCGCCCGTCGCCGACTGCGC
GGCGCGCGCGGACGACATCGCGTGCTTCCAGCTCTCGGGCGGCACGACGGGCACGCCGAAACTGATTCCGCGCCGCCATC
GCGAGTACCTGTACAACGTGCGCGCGTGCTCGGACGCGAGCGGCTTCGGCGCCGACACCGTCTATCTGGCCGCGCTGCCG
ATGGCGCACAACTTCACGCTGTGCTGCCCCGGCGTGATCGGCGCGCTGCTCGCGGGCGGCCGCGTCGTCGCGACCGAGCA
CCCCGAGCCGGAGTGCGGCTTTGCGCTGATCGCGCGCGAGCGGGTCACGCATACGGCGCTCGTCCCGCCGCTCGCGCTAC
TGTGGCTGGACGCGCAGCGCGAGCGCCGAGCGGACCTGTCGAGCCTGCGCGTGCTGCAGGTCGGCGGCGCGCGGCTGATG
GACCACGCGGCCGAACGCGTGACGCCCGTCCTCGGCTGCCGGCTGCAACAGGTATTCGGAATGGCCGAAGGCCTCATCTG
CTGCACGCGGCTCGACGATCCGCCCGCGCGCATCGCGCGTACGCAGGGCCGGCCGGTGTCGCCCGCCGACGAAGTGCGCA
TCGTCGACGAGGCGGGCCGCGCCGTCGCGCCTGGCGAGATCGGCGAATTGCAGGTGCGCGGGCCCTATACGATCCGCGGC
TACTACCGGCTCGCCGAGCATCATGCGGCCGCGTTCACGGCCGACGGCTTCTATCGCAGCGGCGACCGCGTGCGCCGCAC
CGAAGAAGGCGACCTCGTCGTCGAGGGCCGCGACAAGGACCAGATCAATCGCGGCGGCGAAAAAGTATCCGCCGAGGAAG
TCGAGAACCTGCTGCTCGCGCATTCGCAAATCCGCGACGCGGCGCTCGTCGCGATGCCCGATCCGCTGCTCGGCGAGCGC
ACGTGCGCGTTCGTCGTCGCGCGCCCGCCCGCGCCGACCTCGCTCGCGCTGAAGCGGCATTTGCGCGACCACGGGCTCGC
CGCGTTCAAGATTCCCGATCGCATCGAATTCGTGCCGAGCTTTCCTGAAACCGGCATCGGCAAGACCAGCAAGAAATCGC
TGCGCGACCTGCTGCGCCGCCGGCTCGAAGCGGCGCGCGCATGA

Upstream 100 bases:

>100_bases
CGATTCGCCGGCCGCGTCTCCCACGGATTCGCCCGCCTGCGCCCCCAACGTTTCTCCGAACGTTTCCCCAAACGTTTCTC
CCGCCGGCTCGCCGTCGCCG

Downstream 100 bases:

>100_bases
AGCCGCCGCCTCCGCACGCCGGCTGGGTTCGCGAACTCAGGCTGTCGCCCTGCCCGCGCGCGCAGCTCGTCTGCATGCCG
CACGCGGGCGGCGCCGCAAG

Product: salicyl-AMP ligase

Products: (2,3-Dihydroxybenzoyl)adenylate; Diphosphate [C]

Alternate protein names: Dihydroxybenzoic acid-activating enzyme [H]

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MPAAARNAASSDAPDWPDDFARRYRAAGYWRDETFYGALADRARRSPAATAVVDGERRVSYRALLERIRRLAGGLRRLGL
ARGDTAVVHLPNGVRFIEACFALFQLGVRPVLALPAHRQHEIGAFCRFTNARAYLGAARLGEFDSHPLAHALRASCPALA
HIVVAGDDHAFLHFDSLYDAPPVADCAARADDIACFQLSGGTTGTPKLIPRRHREYLYNVRACSDASGFGADTVYLAALP
MAHNFTLCCPGVIGALLAGGRVVATEHPEPECGFALIARERVTHTALVPPLALLWLDAQRERRADLSSLRVLQVGGARLM
DHAAERVTPVLGCRLQQVFGMAEGLICCTRLDDPPARIARTQGRPVSPADEVRIVDEAGRAVAPGEIGELQVRGPYTIRG
YYRLAEHHAAAFTADGFYRSGDRVRRTEEGDLVVEGRDKDQINRGGEKVSAEEVENLLLAHSQIRDAALVAMPDPLLGER
TCAFVVARPPAPTSLALKRHLRDHGLAAFKIPDRIEFVPSFPETGIGKTSKKSLRDLLRRRLEAARA

Sequences:

>Translated_547_residues
MPAAARNAASSDAPDWPDDFARRYRAAGYWRDETFYGALADRARRSPAATAVVDGERRVSYRALLERIRRLAGGLRRLGL
ARGDTAVVHLPNGVRFIEACFALFQLGVRPVLALPAHRQHEIGAFCRFTNARAYLGAARLGEFDSHPLAHALRASCPALA
HIVVAGDDHAFLHFDSLYDAPPVADCAARADDIACFQLSGGTTGTPKLIPRRHREYLYNVRACSDASGFGADTVYLAALP
MAHNFTLCCPGVIGALLAGGRVVATEHPEPECGFALIARERVTHTALVPPLALLWLDAQRERRADLSSLRVLQVGGARLM
DHAAERVTPVLGCRLQQVFGMAEGLICCTRLDDPPARIARTQGRPVSPADEVRIVDEAGRAVAPGEIGELQVRGPYTIRG
YYRLAEHHAAAFTADGFYRSGDRVRRTEEGDLVVEGRDKDQINRGGEKVSAEEVENLLLAHSQIRDAALVAMPDPLLGER
TCAFVVARPPAPTSLALKRHLRDHGLAAFKIPDRIEFVPSFPETGIGKTSKKSLRDLLRRRLEAARA
>Mature_546_residues
PAAARNAASSDAPDWPDDFARRYRAAGYWRDETFYGALADRARRSPAATAVVDGERRVSYRALLERIRRLAGGLRRLGLA
RGDTAVVHLPNGVRFIEACFALFQLGVRPVLALPAHRQHEIGAFCRFTNARAYLGAARLGEFDSHPLAHALRASCPALAH
IVVAGDDHAFLHFDSLYDAPPVADCAARADDIACFQLSGGTTGTPKLIPRRHREYLYNVRACSDASGFGADTVYLAALPM
AHNFTLCCPGVIGALLAGGRVVATEHPEPECGFALIARERVTHTALVPPLALLWLDAQRERRADLSSLRVLQVGGARLMD
HAAERVTPVLGCRLQQVFGMAEGLICCTRLDDPPARIARTQGRPVSPADEVRIVDEAGRAVAPGEIGELQVRGPYTIRGY
YRLAEHHAAAFTADGFYRSGDRVRRTEEGDLVVEGRDKDQINRGGEKVSAEEVENLLLAHSQIRDAALVAMPDPLLGERT
CAFVVARPPAPTSLALKRHLRDHGLAAFKIPDRIEFVPSFPETGIGKTSKKSLRDLLRRRLEAARA

Specific function: Activation of the carboxylate group of 2,3-dihydroxy- benzoate (DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate [H]

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=506, Percent_Identity=25.4940711462451, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI115511026, Length=576, Percent_Identity=24.3055555555556, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI156151445, Length=563, Percent_Identity=22.9129662522202, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI187761345, Length=534, Percent_Identity=27.5280898876405, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI187761343, Length=534, Percent_Identity=27.5280898876405, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI122937307, Length=513, Percent_Identity=25.7309941520468, Blast_Score=110, Evalue=4e-24,
Organism=Homo sapiens, GI157311624, Length=539, Percent_Identity=23.747680890538, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI157311622, Length=539, Percent_Identity=23.747680890538, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI28416953, Length=561, Percent_Identity=24.5989304812834, Blast_Score=106, Evalue=5e-23,
Organism=Homo sapiens, GI58082049, Length=541, Percent_Identity=23.1053604436229, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI42544132, Length=505, Percent_Identity=22.3762376237624, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI8923896, Length=546, Percent_Identity=22.3443223443223, Blast_Score=73, Evalue=7e-13,
Organism=Homo sapiens, GI253970489, Length=127, Percent_Identity=32.2834645669291, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1786810, Length=534, Percent_Identity=45.5056179775281, Blast_Score=447, Evalue=1e-126,
Organism=Escherichia coli, GI145693145, Length=531, Percent_Identity=30.5084745762712, Blast_Score=202, Evalue=5e-53,
Organism=Escherichia coli, GI1788107, Length=549, Percent_Identity=25.6830601092896, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI221142682, Length=505, Percent_Identity=27.1287128712871, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1789201, Length=369, Percent_Identity=25.4742547425474, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1786529, Length=542, Percent_Identity=23.6162361623616, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1790505, Length=525, Percent_Identity=23.047619047619, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1788595, Length=494, Percent_Identity=23.6842105263158, Blast_Score=74, Evalue=3e-14,
Organism=Escherichia coli, GI1786801, Length=466, Percent_Identity=22.5321888412017, Blast_Score=71, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17560308, Length=555, Percent_Identity=27.7477477477477, Blast_Score=148, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17531443, Length=532, Percent_Identity=25.187969924812, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI17538037, Length=542, Percent_Identity=25.4612546125461, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17557194, Length=523, Percent_Identity=25.6214149139579, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71994694, Length=537, Percent_Identity=25.1396648044693, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71994690, Length=537, Percent_Identity=25.1396648044693, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71994703, Length=537, Percent_Identity=25.1396648044693, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17559526, Length=367, Percent_Identity=26.4305177111717, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32563687, Length=512, Percent_Identity=22.4609375, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17558820, Length=350, Percent_Identity=25.7142857142857, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17560140, Length=311, Percent_Identity=26.3665594855305, Blast_Score=82, Evalue=9e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=358, Percent_Identity=26.2569832402235, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71982997, Length=544, Percent_Identity=23.8970588235294, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI71983001, Length=544, Percent_Identity=23.8970588235294, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17556356, Length=356, Percent_Identity=24.1573033707865, Blast_Score=69, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=360, Percent_Identity=26.1111111111111, Blast_Score=130, Evalue=5e-31,
Organism=Drosophila melanogaster, GI24581924, Length=532, Percent_Identity=25.9398496240602, Blast_Score=145, Evalue=1e-34,
Organism=Drosophila melanogaster, GI21355181, Length=520, Percent_Identity=24.6153846153846, Blast_Score=115, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19922652, Length=495, Percent_Identity=24.8484848484848, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI18859661, Length=525, Percent_Identity=24, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24656500, Length=498, Percent_Identity=24.4979919678715, Blast_Score=100, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24667955, Length=549, Percent_Identity=22.5865209471767, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI62472339, Length=549, Percent_Identity=22.5865209471767, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24648260, Length=360, Percent_Identity=22.7777777777778, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24648253, Length=511, Percent_Identity=22.3091976516634, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24667959, Length=407, Percent_Identity=23.8329238329238, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356947, Length=351, Percent_Identity=22.7920227920228, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24653035, Length=360, Percent_Identity=22.2222222222222, Blast_Score=73, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 2.7.7.58; 2.3.1.- [C]

Molecular weight: Translated: 59630; Mature: 59499

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPAAARNAASSDAPDWPDDFARRYRAAGYWRDETFYGALADRARRSPAATAVVDGERRVS
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCCHHHH
YRALLERIRRLAGGLRRLGLARGDTAVVHLPNGVRFIEACFALFQLGVRPVLALPAHRQH
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEECCCCCCC
EIGAFCRFTNARAYLGAARLGEFDSHPLAHALRASCPALAHIVVAGDDHAFLHFDSLYDA
CHHHHEEECCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEHHHCCC
PPVADCAARADDIACFQLSGGTTGTPKLIPRRHREYLYNVRACSDASGFGADTVYLAALP
CCHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEC
MAHNFTLCCPGVIGALLAGGRVVATEHPEPECGFALIARERVTHTALVPPLALLWLDAQR
CCCCCEEECHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
ERRADLSSLRVLQVGGARLMDHAAERVTPVLGCRLQQVFGMAEGLICCTRLDDPPARIAR
HHHCCHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHCCCEEEECCCCCHHHHHH
TQGRPVSPADEVRIVDEAGRAVAPGEIGELQVRGPYTIRGYYRLAEHHAAAFTADGFYRS
CCCCCCCCHHHEEEEHHCCCEECCCCCCEEEECCCEEEEHHHHHHHHHHHEEEECCCCCC
GDRVRRTEEGDLVVEGRDKDQINRGGEKVSAEEVENLLLAHSQIRDAALVAMPDPLLGER
CCHHEECCCCCEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCC
TCAFVVARPPAPTSLALKRHLRDHGLAAFKIPDRIEFVPSFPETGIGKTSKKSLRDLLRR
EEEEEEECCCCCHHHHHHHHHHHCCEEEEECCCHHCCCCCCCCCCCCCCHHHHHHHHHHH
RLEAARA
HHHHHCC
>Mature Secondary Structure 
PAAARNAASSDAPDWPDDFARRYRAAGYWRDETFYGALADRARRSPAATAVVDGERRVS
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCCHHHH
YRALLERIRRLAGGLRRLGLARGDTAVVHLPNGVRFIEACFALFQLGVRPVLALPAHRQH
HHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCEEECCCCCCC
EIGAFCRFTNARAYLGAARLGEFDSHPLAHALRASCPALAHIVVAGDDHAFLHFDSLYDA
CHHHHEEECCCHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEECCCCEEEEEHHHCCC
PPVADCAARADDIACFQLSGGTTGTPKLIPRRHREYLYNVRACSDASGFGADTVYLAALP
CCHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEC
MAHNFTLCCPGVIGALLAGGRVVATEHPEPECGFALIARERVTHTALVPPLALLWLDAQR
CCCCCEEECHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHH
ERRADLSSLRVLQVGGARLMDHAAERVTPVLGCRLQQVFGMAEGLICCTRLDDPPARIAR
HHHCCHHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCHHCCCEEEECCCCCHHHHHH
TQGRPVSPADEVRIVDEAGRAVAPGEIGELQVRGPYTIRGYYRLAEHHAAAFTADGFYRS
CCCCCCCCHHHEEEEHHCCCEECCCCCCEEEECCCEEEEHHHHHHHHHHHEEEECCCCCC
GDRVRRTEEGDLVVEGRDKDQINRGGEKVSAEEVENLLLAHSQIRDAALVAMPDPLLGER
CCHHEECCCCCEEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCC
TCAFVVARPPAPTSLALKRHLRDHGLAAFKIPDRIEFVPSFPETGIGKTSKKSLRDLLRR
EEEEEEECCCCCHHHHHHHHHHHCCEEEEECCCHHCCCCCCCCCCCCCCHHHHHHHHHHH
RLEAARA
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2,3-Dihydroxybenzoate; ATP [C]

Specific reaction: 2,3-Dihydroxybenzoate + ATP <==> (2,3-Dihydroxybenzoyl)adenylate + Diphosphate [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8921902; 9384377; 8224884; 12221282 [H]