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The map label for this gene is rbsB [H]

Identifier: 53721176

GI number: 53721176

Start: 180870

End: 181817

Strand: Reverse

Name: rbsB [H]

Synonym: BPSS0140

Alternate gene names: 53721176

Gene position: 181817-180870 (Counterclockwise)

Preceding gene: 53721177

Following gene: 53721172

Centisome position: 5.73

GC content: 65.93

Gene sequence:

>948_bases
ATGTTGGAGATCAAGCACAAGCTCGTTCAGACCGCCGCCGCGTGCGCGCTCGCGTTCGCCGCGTGCGCCGCGCACGCGGC
CGACAAACCGCTCAAGTCGATCGGCATCACCGTCGGCTCGCTCGGCAATCCGTACTTCGTGACCGTCGTGAAAGGCGCGC
AGGCGCAGGCGAAGGAAATCAATCCGAGCGCGAAGGTCACGGCGGTATCCGCCGATTACGATCTGAACAAGCAGTTCACG
CAGATCGACAACTTCATCTCGGCGCACGTCGACATGATCCTGCTGAACGCGGCCGACCCGAAGGCGATCGAGCCCGCCGT
GCGCAAGGCGCAGGCGGCGGGCATCGCGGTGATCGCGGTCGACGTCGCGGCGTCCGGCGCGGACGCGACCGTGCAGACGA
ACAACGTGAAGGCGGGCGAGCTCGCGTGCGATTACCTCGCGAAGAAGCTCAACGGCAAGGGCAACGTGATCATCGAGAAC
GGCCCGCAGGTGTCGGCCGTCGTCGATCGCGTGAACGGCTGCAAGGCGGTGCTCGCGAAGAATCCGGGCCTCAGGCTGCT
ATCGAGCGACCAGGACGGCAAGGGCTCGCGCGAAGGCGGCATGAACGCGATGCAGGGCTACCTCACGCGCTTCCCGAAAG
TCGACGGGCTCTTCGCGATCAACGATCCGCAGGCGATCGGCAGCGATCTCGCCGCGAAGCAGTTGCGCCGCACCGGCATC
GTGATCACGTCGGTCGACGGCGCGCCCGACATCGAAACCGCGCTGACGAGCGACACGCAGGTGCAGGCGTCGTCGAGCCA
GGACCCGTTCGCGATGGCGAAGAAGGCCGTTTCGGTCGGCTACGGCATCATGAACGGGCACAAGCCCGCGAACGCGATGA
TCCTGCTCGAGCCGACGCTCATCACGCGCGAGAACGTGAAGCGCTACAAGGGCTGGAGCGCGCATTGA

Upstream 100 bases:

>100_bases
CAGCACGGATCGGCCCGCACCTGAGCGCATCGGCCGCCATGCGGCGCGCGCGCAGGTCCTCGCGCGCGCCCTCCATCCCG
GACCATACGGAGACAACGAC

Downstream 100 bases:

>100_bases
CCGCCATGCCGCCGCCGTCCGGGCGGCGGCACGGTACACGGCACACGGAACGGCAAGGCACCGCACGACGCGATGCGGCG
CGCGGCCGGACGGCGCGCCG

Product: sugar ABC transport system, lipoprotein

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 315; Mature: 315

Protein sequence:

>315_residues
MLEIKHKLVQTAAACALAFAACAAHAADKPLKSIGITVGSLGNPYFVTVVKGAQAQAKEINPSAKVTAVSADYDLNKQFT
QIDNFISAHVDMILLNAADPKAIEPAVRKAQAAGIAVIAVDVAASGADATVQTNNVKAGELACDYLAKKLNGKGNVIIEN
GPQVSAVVDRVNGCKAVLAKNPGLRLLSSDQDGKGSREGGMNAMQGYLTRFPKVDGLFAINDPQAIGSDLAAKQLRRTGI
VITSVDGAPDIETALTSDTQVQASSSQDPFAMAKKAVSVGYGIMNGHKPANAMILLEPTLITRENVKRYKGWSAH

Sequences:

>Translated_315_residues
MLEIKHKLVQTAAACALAFAACAAHAADKPLKSIGITVGSLGNPYFVTVVKGAQAQAKEINPSAKVTAVSADYDLNKQFT
QIDNFISAHVDMILLNAADPKAIEPAVRKAQAAGIAVIAVDVAASGADATVQTNNVKAGELACDYLAKKLNGKGNVIIEN
GPQVSAVVDRVNGCKAVLAKNPGLRLLSSDQDGKGSREGGMNAMQGYLTRFPKVDGLFAINDPQAIGSDLAAKQLRRTGI
VITSVDGAPDIETALTSDTQVQASSSQDPFAMAKKAVSVGYGIMNGHKPANAMILLEPTLITRENVKRYKGWSAH
>Mature_315_residues
MLEIKHKLVQTAAACALAFAACAAHAADKPLKSIGITVGSLGNPYFVTVVKGAQAQAKEINPSAKVTAVSADYDLNKQFT
QIDNFISAHVDMILLNAADPKAIEPAVRKAQAAGIAVIAVDVAASGADATVQTNNVKAGELACDYLAKKLNGKGNVIIEN
GPQVSAVVDRVNGCKAVLAKNPGLRLLSSDQDGKGSREGGMNAMQGYLTRFPKVDGLFAINDPQAIGSDLAAKQLRRTGI
VITSVDGAPDIETALTSDTQVQASSSQDPFAMAKKAVSVGYGIMNGHKPANAMILLEPTLITRENVKRYKGWSAH

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=286, Percent_Identity=29.7202797202797, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1790674, Length=205, Percent_Identity=27.8048780487805, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32863; Mature: 32863

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEIKHKLVQTAAACALAFAACAAHAADKPLKSIGITVGSLGNPYFVTVVKGAQAQAKEI
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCEEEECCCCCEEEEEEECCCCHHHHC
NPSAKVTAVSADYDLNKQFTQIDNFISAHVDMILLNAADPKAIEPAVRKAQAAGIAVIAV
CCCCEEEEEECCCCCCHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHCCEEEEEE
DVAASGADATVQTNNVKAGELACDYLAKKLNGKGNVIIENGPQVSAVVDRVNGCKAVLAK
EEECCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCEEEEEC
NPGLRLLSSDQDGKGSREGGMNAMQGYLTRFPKVDGLFAINDPQAIGSDLAAKQLRRTGI
CCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHCCE
VITSVDGAPDIETALTSDTQVQASSSQDPFAMAKKAVSVGYGIMNGHKPANAMILLEPTL
EEEECCCCCCCHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCC
ITRENVKRYKGWSAH
CCHHHHHHCCCCCCC
>Mature Secondary Structure
MLEIKHKLVQTAAACALAFAACAAHAADKPLKSIGITVGSLGNPYFVTVVKGAQAQAKEI
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCEEEECCCCCEEEEEEECCCCHHHHC
NPSAKVTAVSADYDLNKQFTQIDNFISAHVDMILLNAADPKAIEPAVRKAQAAGIAVIAV
CCCCEEEEEECCCCCCHHHHHHHHHHHHCEEEEEEECCCCCHHHHHHHHHHHCCEEEEEE
DVAASGADATVQTNNVKAGELACDYLAKKLNGKGNVIIENGPQVSAVVDRVNGCKAVLAK
EEECCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCCEEEEEC
NPGLRLLSSDQDGKGSREGGMNAMQGYLTRFPKVDGLFAINDPQAIGSDLAAKQLRRTGI
CCCCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHCCE
VITSVDGAPDIETALTSDTQVQASSSQDPFAMAKKAVSVGYGIMNGHKPANAMILLEPTL
EEEECCCCCCCHHHHCCCCEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCC
ITRENVKRYKGWSAH
CCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]