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The map label for this gene is livM [H]

Identifier: 53721021

GI number: 53721021

Start: 4054658

End: 4055635

Strand: Reverse

Name: livM [H]

Synonym: BPSL3414

Alternate gene names: 53721021

Gene position: 4055635-4054658 (Counterclockwise)

Preceding gene: 53721022

Following gene: 53721020

Centisome position: 99.54

GC content: 66.56

Gene sequence:

>978_bases
ATGATGCAGAGAAAAGCGCTCTACGGCCTGCTGCTGCTCGGCCTGCTCGTCGCGCCGTTCGTCGGCGCGTATCCGGTGTT
CGTGATGAAGGTGCTGTGTTTCGCGTTGTTCGCGGCGGCGTTCAATCTGCTGATCGGCTTCACGGGGTTGCTGTCGTTCG
GCCACGCGATGTTTCTCGCGACCGCGGGCTACGTGACGGGTTACGCGATGCAGTCGCTCGGCACGGCGCCCGAGCTCGGC
GTGCTGGCCGGCACCGCCGCCGCGACGCTGCTCGGCCTCGTCGTCGGCCTGTTCGCGATCCGGCGGCAGGGCATCTATTT
CGCGATGATCACGCTCGCGTTCGCCCAGATGGTGTATTTCATCTATCTGCAAGCGCCGTTCACGCACGGCGAGGACGGCC
TGCAGGGCGTGCCGCGCGGGCGGCTGTTCGGGCTCGTCGATCTGTCGTCCGATCTCGCGCTCTACTATGTGGTGCTCGTC
GTGATCGCGTGCGCGTGCTTCTTCATCGTGCGCATCGTGCATTCGCCGTTCGGGCAGGTGCTTGTCGCGATCAAGGAGAA
CGAGCCTCGCGCGATATCGCTCGGCTACGATACCGATCGCTTCAAGCTGCTTGCGTTCATCCTGTCGGCCGCGCTCGCGG
GGCTTGCCGGCGCGCTGAAAGTGGTCGTGCTCGGCTTCGAGACGCTGAGCGATGCGTACTGGACGATGTCGGGCCTCGTC
GTGCTGATGACGCTCGTGGGCGGCATGGGCACGCTGTTCGGCCCGCTCGTCGGCGCGGCGCTGATCGTCGCGCTCGAGGA
TCGGCTGGGCGACATCGGCGGCTGGCTCGCGTCGGTCACGCACATCGACTGGTTCCGCTCGCTCGGCGAATCCGCGACGA
TCGTCACCGGCCTTATCTTCATCGTGTGCGTGCTCGCGTTCAGGCGCGGCATCGTCGGCGAGGTGATCGCCCGGGTGCGG
CCGCTGCGCGCGTCGTGA

Upstream 100 bases:

>100_bases
TGACGCGCGTGTTCTATCCGGAAGCATCGGCGACGGTCGTCTTCGTGATCATGGCGATCGTGCTGTTGATTCGTCCGGCA
GGTTTGTTCGGCAAGGAAAG

Downstream 100 bases:

>100_bases
ATGGCGAGGACGCGCGATCGTGCGGTGCGCAATGATCGGCGGCCATCCGGTGCGATGCGCGCCGCGAACGGCCGGCCCGG
TTCGGCCGGACATGGCCGCC

Product: putative branched-chain amino acid ABC transporter integral membrane subunit

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein M [H]

Number of amino acids: Translated: 325; Mature: 325

Protein sequence:

>325_residues
MMQRKALYGLLLLGLLVAPFVGAYPVFVMKVLCFALFAAAFNLLIGFTGLLSFGHAMFLATAGYVTGYAMQSLGTAPELG
VLAGTAAATLLGLVVGLFAIRRQGIYFAMITLAFAQMVYFIYLQAPFTHGEDGLQGVPRGRLFGLVDLSSDLALYYVVLV
VIACACFFIVRIVHSPFGQVLVAIKENEPRAISLGYDTDRFKLLAFILSAALAGLAGALKVVVLGFETLSDAYWTMSGLV
VLMTLVGGMGTLFGPLVGAALIVALEDRLGDIGGWLASVTHIDWFRSLGESATIVTGLIFIVCVLAFRRGIVGEVIARVR
PLRAS

Sequences:

>Translated_325_residues
MMQRKALYGLLLLGLLVAPFVGAYPVFVMKVLCFALFAAAFNLLIGFTGLLSFGHAMFLATAGYVTGYAMQSLGTAPELG
VLAGTAAATLLGLVVGLFAIRRQGIYFAMITLAFAQMVYFIYLQAPFTHGEDGLQGVPRGRLFGLVDLSSDLALYYVVLV
VIACACFFIVRIVHSPFGQVLVAIKENEPRAISLGYDTDRFKLLAFILSAALAGLAGALKVVVLGFETLSDAYWTMSGLV
VLMTLVGGMGTLFGPLVGAALIVALEDRLGDIGGWLASVTHIDWFRSLGESATIVTGLIFIVCVLAFRRGIVGEVIARVR
PLRAS
>Mature_325_residues
MMQRKALYGLLLLGLLVAPFVGAYPVFVMKVLCFALFAAAFNLLIGFTGLLSFGHAMFLATAGYVTGYAMQSLGTAPELG
VLAGTAAATLLGLVVGLFAIRRQGIYFAMITLAFAQMVYFIYLQAPFTHGEDGLQGVPRGRLFGLVDLSSDLALYYVVLV
VIACACFFIVRIVHSPFGQVLVAIKENEPRAISLGYDTDRFKLLAFILSAALAGLAGALKVVVLGFETLSDAYWTMSGLV
VLMTLVGGMGTLFGPLVGAALIVALEDRLGDIGGWLASVTHIDWFRSLGESATIVTGLIFIVCVLAFRRGIVGEVIARVR
PLRAS

Specific function: Part of the binding-protein-dependent transport system for branched-chain amino acids. Probably responsible for the translocation of the substrates across the membrane [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=258, Percent_Identity=29.0697674418605, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 34621; Mature: 34621

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMQRKALYGLLLLGLLVAPFVGAYPVFVMKVLCFALFAAAFNLLIGFTGLLSFGHAMFLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAGYVTGYAMQSLGTAPELGVLAGTAAATLLGLVVGLFAIRRQGIYFAMITLAFAQMVYF
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IYLQAPFTHGEDGLQGVPRGRLFGLVDLSSDLALYYVVLVVIACACFFIVRIVHSPFGQV
HHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHE
LVAIKENEPRAISLGYDTDRFKLLAFILSAALAGLAGALKVVVLGFETLSDAYWTMSGLV
EEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLMTLVGGMGTLFGPLVGAALIVALEDRLGDIGGWLASVTHIDWFRSLGESATIVTGLIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IVCVLAFRRGIVGEVIARVRPLRAS
HHHHHHHHCCHHHHHHHHHCCCCCC
>Mature Secondary Structure
MMQRKALYGLLLLGLLVAPFVGAYPVFVMKVLCFALFAAAFNLLIGFTGLLSFGHAMFLA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TAGYVTGYAMQSLGTAPELGVLAGTAAATLLGLVVGLFAIRRQGIYFAMITLAFAQMVYF
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
IYLQAPFTHGEDGLQGVPRGRLFGLVDLSSDLALYYVVLVVIACACFFIVRIVHSPFGQV
HHHCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHE
LVAIKENEPRAISLGYDTDRFKLLAFILSAALAGLAGALKVVVLGFETLSDAYWTMSGLV
EEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VLMTLVGGMGTLFGPLVGAALIVALEDRLGDIGGWLASVTHIDWFRSLGESATIVTGLIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
IVCVLAFRRGIVGEVIARVRPLRAS
HHHHHHHHCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]