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The map label for this gene is flhB [H]

Identifier: 53720905

GI number: 53720905

Start: 3913455

End: 3914672

Strand: Reverse

Name: flhB [H]

Synonym: BPSL3295

Alternate gene names: 53720905

Gene position: 3914672-3913455 (Counterclockwise)

Preceding gene: 53720906

Following gene: 53720904

Centisome position: 96.08

GC content: 69.05

Gene sequence:

>1218_bases
ATGGAGGCCCGCGTGGCAGAGGACAGCGATCTCGACCGGACAGAAGCCGCCACTCCCAAGCGCCGCGAGAAGGCGCGCGA
GGAGGGGCAGGTCGCGCGCTCGCGCGAACTGGCCTCTTTCGCGCTGCTGTCGGCGGGCTTCTACGGCGCATGGATGCTGT
CCGGGCCGATCGGCGAGCATCTGCGCACCATGCTGCACACCGCATTCTCGTTCGACCGCGCAGCCGCGTTCGATACGAAC
CGGATGCTGTCGCACGCGGGCACGCTGAGCCTCGAAGGGCTCTACGCGCTCGCGCCCGTGCTCGCGCTCACGGGCGTCGC
GGCGCTCGCCGCGCCGATGGCGATGGGCGGCTGGCTCGTGTCGACGAAGACGTTCGAGCTGAAGTTCGAGCGCCTGAACC
CGATCACGGGCCTCGGCCGCATTTTCTCGATTCAGGGCCCGATTCAACTCGGGATGTCGATCGCGAAGACGCTCGTCGTC
GGCGGAATCGGCGGCATCGCGATCTGGCGCAGCAAGGACGAATTGCTCGGCCTCGCGACGCAGCCGCTTCACGCGGCGCT
CGCCGACGCGCTGCACCTCGTCGCCGTGTGCTGCGGGATGACGGTTGCGGGGATGCTCGTCGTCGCCGGCCTCGACGTGC
CGTACCAACTCTGGCAGTACAACAAGAAGCTGCGCATGACGAAGGAAGAAGTAAAGCGCGAGCACCGCGAGAACGAAGGC
GATCCGCACGTGAAGGGCCGCATCCGCCAGCAGCAGCGCGCGATGGCGCGCCGCCGGATGATGGCGAACGTGCCGACGGC
CGACGTCGTCGTCACGAACCCGACGCACTTCGCGGTCGCGCTCAAGTACACGGATGGCGAGATGCGCGCGCCGAAGGTCG
TCGCCAAGGGCGTGAACCTCGTCGCCGCGCGCATCCGCGAGCTCGCGGCCGAGCACCACGTGCCGCTGCTCGAAGCGCCG
CCGCTCGCGCGTGCGCTCTATCACAACGTCGAACTCGAGCGCGAGATTCCCGGCACGCTGTATTCGGCCGTCGCCGAAGT
GCTCGCGTGGGTGTATCAGCTCAAGCGCTTCCGCGCCGAAGGTGGGATCGCGCCCGAGACGCCCGTCGATCTCGACGTGC
CGGCCGAACTCGACAAGGGCGCGCACGTCGCGCCGCAAGACGAACGCGAAGAGGCCGAAGACGCGCTCGGCGGCCGCGCC
GCCGGAGGTGCCGCATGA

Upstream 100 bases:

>100_bases
CGAGAAATACCCCGGTTTCCCCGCTTTCCTCGACCGATCGCCCGTTGACCGCCGCGGGAATAATCGGTGTCACTGGAAAG
CGGCATCCCGCCGCGCCCAC

Downstream 100 bases:

>100_bases
ACATGCCGGCCGGACTCCTCGGCAAGCGCGCGCAACTGTTCGCGGGCACGAACCTGCGCGCGCTCGCGGGCCCGATCCTC
ATCTGCATGATTCTGGGGAT

Product: flagellar biosynthesis protein FlhB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MEARVAEDSDLDRTEAATPKRREKAREEGQVARSRELASFALLSAGFYGAWMLSGPIGEHLRTMLHTAFSFDRAAAFDTN
RMLSHAGTLSLEGLYALAPVLALTGVAALAAPMAMGGWLVSTKTFELKFERLNPITGLGRIFSIQGPIQLGMSIAKTLVV
GGIGGIAIWRSKDELLGLATQPLHAALADALHLVAVCCGMTVAGMLVVAGLDVPYQLWQYNKKLRMTKEEVKREHRENEG
DPHVKGRIRQQQRAMARRRMMANVPTADVVVTNPTHFAVALKYTDGEMRAPKVVAKGVNLVAARIRELAAEHHVPLLEAP
PLARALYHNVELEREIPGTLYSAVAEVLAWVYQLKRFRAEGGIAPETPVDLDVPAELDKGAHVAPQDEREEAEDALGGRA
AGGAA

Sequences:

>Translated_405_residues
MEARVAEDSDLDRTEAATPKRREKAREEGQVARSRELASFALLSAGFYGAWMLSGPIGEHLRTMLHTAFSFDRAAAFDTN
RMLSHAGTLSLEGLYALAPVLALTGVAALAAPMAMGGWLVSTKTFELKFERLNPITGLGRIFSIQGPIQLGMSIAKTLVV
GGIGGIAIWRSKDELLGLATQPLHAALADALHLVAVCCGMTVAGMLVVAGLDVPYQLWQYNKKLRMTKEEVKREHRENEG
DPHVKGRIRQQQRAMARRRMMANVPTADVVVTNPTHFAVALKYTDGEMRAPKVVAKGVNLVAARIRELAAEHHVPLLEAP
PLARALYHNVELEREIPGTLYSAVAEVLAWVYQLKRFRAEGGIAPETPVDLDVPAELDKGAHVAPQDEREEAEDALGGRA
AGGAA
>Mature_405_residues
MEARVAEDSDLDRTEAATPKRREKAREEGQVARSRELASFALLSAGFYGAWMLSGPIGEHLRTMLHTAFSFDRAAAFDTN
RMLSHAGTLSLEGLYALAPVLALTGVAALAAPMAMGGWLVSTKTFELKFERLNPITGLGRIFSIQGPIQLGMSIAKTLVV
GGIGGIAIWRSKDELLGLATQPLHAALADALHLVAVCCGMTVAGMLVVAGLDVPYQLWQYNKKLRMTKEEVKREHRENEG
DPHVKGRIRQQQRAMARRRMMANVPTADVVVTNPTHFAVALKYTDGEMRAPKVVAKGVNLVAARIRELAAEHHVPLLEAP
PLARALYHNVELEREIPGTLYSAVAEVLAWVYQLKRFRAEGGIAPETPVDLDVPAELDKGAHVAPQDEREEAEDALGGRA
AGGAA

Specific function: Required for formation of the rod structure in the basal body of the flagellar apparatus. Together with fliI and fliH, may constitute the export apparatus of flagellin [H]

COG id: COG1377

COG function: function code NU; Flagellar biosynthesis pathway, component FlhB

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type III secretion exporter family [H]

Homologues:

Organism=Escherichia coli, GI1788188, Length=374, Percent_Identity=47.5935828877005, Blast_Score=321, Evalue=6e-89,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006136
- InterPro:   IPR006135 [H]

Pfam domain/function: PF01312 Bac_export_2 [H]

EC number: NA

Molecular weight: Translated: 43895; Mature: 43895

Theoretical pI: Translated: 7.44; Mature: 7.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEARVAEDSDLDRTEAATPKRREKAREEGQVARSRELASFALLSAGFYGAWMLSGPIGEH
CCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEECCCHHHH
LRTMLHTAFSFDRAAAFDTNRMLSHAGTLSLEGLYALAPVLALTGVAALAAPMAMGGWLV
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEE
STKTFELKFERLNPITGLGRIFSIQGPIQLGMSIAKTLVVGGIGGIAIWRSKDELLGLAT
ECEEEEEEHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHCCCC
QPLHAALADALHLVAVCCGMTVAGMLVVAGLDVPYQLWQYNKKLRMTKEEVKREHRENEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHCCHHHHHHHHCCCCC
DPHVKGRIRQQQRAMARRRMMANVPTADVVVTNPTHFAVALKYTDGEMRAPKVVAKGVNL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEEEECCCCCCCCHHHHHHHHH
VAARIRELAAEHHVPLLEAPPLARALYHNVELEREIPGTLYSAVAEVLAWVYQLKRFRAE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GGIAPETPVDLDVPAELDKGAHVAPQDEREEAEDALGGRAAGGAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEARVAEDSDLDRTEAATPKRREKAREEGQVARSRELASFALLSAGFYGAWMLSGPIGEH
CCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHEEECCCHHHH
LRTMLHTAFSFDRAAAFDTNRMLSHAGTLSLEGLYALAPVLALTGVAALAAPMAMGGWLV
HHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEE
STKTFELKFERLNPITGLGRIFSIQGPIQLGMSIAKTLVVGGIGGIAIWRSKDELLGLAT
ECEEEEEEHHHCCCCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCEEEECCHHHHHCCCC
QPLHAALADALHLVAVCCGMTVAGMLVVAGLDVPYQLWQYNKKLRMTKEEVKREHRENEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHHCCHHHHHHHHCCCCC
DPHVKGRIRQQQRAMARRRMMANVPTADVVVTNPTHFAVALKYTDGEMRAPKVVAKGVNL
CCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEEEEEECCCCCCCCHHHHHHHHH
VAARIRELAAEHHVPLLEAPPLARALYHNVELEREIPGTLYSAVAEVLAWVYQLKRFRAE
HHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHC
GGIAPETPVDLDVPAELDKGAHVAPQDEREEAEDALGGRAAGGAA
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8002587; 11677609; 3298217 [H]