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The map label for this gene is hisH [H]

Identifier: 53720742

GI number: 53720742

Start: 3736662

End: 3737303

Strand: Reverse

Name: hisH [H]

Synonym: BPSL3135

Alternate gene names: 53720742

Gene position: 3737303-3736662 (Counterclockwise)

Preceding gene: 53720743

Following gene: 53720741

Centisome position: 91.72

GC content: 66.51

Gene sequence:

>642_bases
ATGAAAACTTCGATTGCAATTGTGGATTACGGAATGGGCAACCTGCGCTCGGTCGCGCAGGCGCTCATGAAGGCGGAGCC
GGCGGCCGACGTGAAGATCGTCGACCGGCCCGAGGCGATTCGCGCGGCCGACCGCGTGGTGCTGCCGGGCCAGGGCGCGA
TGCCCGACTGCATGCGGTGCCTCGGCGAATCCGGCTTGCAGGAGGCGGTGGTCGAGGCGTCGCGCACGAAGCCGCTGCTC
GGCGTATGCGTCGGCGAGCAGATGCTGTTCGACTGGAGCGCGGAAGGCGACACGAAGGGGCTCGGCCTGCTGCCGGGCAA
GGTGGTGCGCTTCGCGCTCGAGGGCCGGCTGCAGGACGACGGCTCGCGCTTCAAGGTGCCGCAGATGGGCTGGAACCGGG
TGCGCCAGACCCAGGCGCATCCGTTGTGGGACGGTGTGCCGGATGACGCCTATTTCTACTTCGTGCACAGTTATTACGTG
GTGCCGGACAACCCGGCGCACACGGCGGGCGAGACCCTGTACGGCGACGTTTTCACGTCCGCGGTCGCGCGCGACAATCT
CTTCGCGACCCAATTTCACCCCGAAAAGAGCGCCGAAGTGGGGCTGCGGCTGTACCGGAATTTTGTCCACTGGAACCCCT
GA

Upstream 100 bases:

>100_bases
CCGGCATCAACATCGTCACGCGTCTGATGGGATTGATGCTGTCGGCGCTGGCGGTGGAATTCATCGTCGACGGACTGAAG
GCATTATTGCCTGCACTGAC

Downstream 100 bases:

>100_bases
ACGTGGCGCGGGAACCACACTCGGCGTGCGCAAAACGCCCGCCGCACGGGCTTCGCGAGCGGATACGCAGAAAGAGTTGT
ACTAAACTAGCGAGACGGCA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKTSIAIVDYGMGNLRSVAQALMKAEPAADVKIVDRPEAIRAADRVVLPGQGAMPDCMRCLGESGLQEAVVEASRTKPLL
GVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFALEGRLQDDGSRFKVPQMGWNRVRQTQAHPLWDGVPDDAYFYFVHSYYV
VPDNPAHTAGETLYGDVFTSAVARDNLFATQFHPEKSAEVGLRLYRNFVHWNP

Sequences:

>Translated_213_residues
MKTSIAIVDYGMGNLRSVAQALMKAEPAADVKIVDRPEAIRAADRVVLPGQGAMPDCMRCLGESGLQEAVVEASRTKPLL
GVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFALEGRLQDDGSRFKVPQMGWNRVRQTQAHPLWDGVPDDAYFYFVHSYYV
VPDNPAHTAGETLYGDVFTSAVARDNLFATQFHPEKSAEVGLRLYRNFVHWNP
>Mature_213_residues
MKTSIAIVDYGMGNLRSVAQALMKAEPAADVKIVDRPEAIRAADRVVLPGQGAMPDCMRCLGESGLQEAVVEASRTKPLL
GVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFALEGRLQDDGSRFKVPQMGWNRVRQTQAHPLWDGVPDDAYFYFVHSYYV
VPDNPAHTAGETLYGDVFTSAVARDNLFATQFHPEKSAEVGLRLYRNFVHWNP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=208, Percent_Identity=36.0576923076923, Blast_Score=120, Evalue=5e-29,
Organism=Saccharomyces cerevisiae, GI6319725, Length=218, Percent_Identity=31.651376146789, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 23513; Mature: 23513

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSIAIVDYGMGNLRSVAQALMKAEPAADVKIVDRPEAIRAADRVVLPGQGAMPDCMRC
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHCEEEECCCCCCHHHHHH
LGESGLQEAVVEASRTKPLLGVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFALEGRLQDD
HCCCCHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCC
GSRFKVPQMGWNRVRQTQAHPLWDGVPDDAYFYFVHSYYVVPDNPAHTAGETLYGDVFTS
CCEEECCCCCHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCHHHHHHHHH
AVARDNLFATQFHPEKSAEVGLRLYRNFVHWNP
HHHHCCEEEEECCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTSIAIVDYGMGNLRSVAQALMKAEPAADVKIVDRPEAIRAADRVVLPGQGAMPDCMRC
CCCEEEEEECCCCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHCEEEECCCCCCHHHHHH
LGESGLQEAVVEASRTKPLLGVCVGEQMLFDWSAEGDTKGLGLLPGKVVRFALEGRLQDD
HCCCCHHHHHHHHHCCCCEEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCC
GSRFKVPQMGWNRVRQTQAHPLWDGVPDDAYFYFVHSYYVVPDNPAHTAGETLYGDVFTS
CCEEECCCCCHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCCCCCHHHHHHHHH
AVARDNLFATQFHPEKSAEVGLRLYRNFVHWNP
HHHHCCEEEEECCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12754231 [H]