Click here to switch to the map view.

The map label for this gene is mpl [H]

Identifier: 53720587

GI number: 53720587

Start: 3552955

End: 3554439

Strand: Reverse

Name: mpl [H]

Synonym: BPSL2979

Alternate gene names: 53720587

Gene position: 3554439-3552955 (Counterclockwise)

Preceding gene: 53720598

Following gene: 53720586

Centisome position: 87.24

GC content: 68.01

Gene sequence:

>1485_bases
ATGTTTTCCGGCAACCGGCGCGACGTGCGGCCGGCTGCCGATTTCGACTACGCCCACGCGGCGCGAAGCTCTATGCATAT
CCACATTCTCGGCATCTGCGGCACCTTCATGGGCGGTCTGGCCGTGCTCGCACGCGCGGCGGGGCATACCGTGACCGGCT
GCGACGCGGGCGTCTATCCGCCAATGAGCACGCAACTCGAGGCGCAGGGTATCCAGCTGATCGAAGGCTACGGCGCCGAA
CAGATCGATCTGAAGCCCGATCTGTTCGTGATCGGCAACGTGGTCTCGCGCGGCAACCCGCTGATGGAGGCGATTCTCGA
TCGCGGGCTGCCGTATGTATCGGGCCCGCAATGGCTCGGCGAGCATGTGCTGGCGGGTAAGTGGGTGCTCGCTGTCGCCG
GTACGCACGGCAAGACGACGACGAGCTCGATGCTCGCGTGGGTACTGGAAGACGCCGGCCTGAATCCGGGTTTCCTGATC
GGTGGCGTGCCGCTGAATTTCGGCGTGTCCGCACGGTTGACCGATTCGAGCTTCTTCGTGATCGAGGCGGACGAGTACGA
CACGGCGTTTTTCGACAAGCGCTCGAAATTCGTCCATTACCGGCCGCGGACCGCGATCCTGAACAATCTCGAATTCGATC
ATGCGGACATCTTCCCGGATCTCGCCGCGATCGAGACGCAATTCCATCATCTGGTGCGCACCGTGCCGGGCGTCGGCCGG
CTCGTGACGAACGGCCGCGACGACGCGCTCGAGCGCGTGCTGTCGCGCGGCTGCTGGAGCGACGTCGAGCGCTTCGGTGT
CGACGGCGGCTGGCAGGCGTTGCCTGTCGAGAATGGCGTGCCGGTGGACGGGCGTTTCGCGGTGTATTGGCGCAGCGAGC
GCGTCGGCGCCGTCGATTGGCAGGTGCAGGGCGAGCACAATCGGATGAACGCGCTCGCGGCGATCGCCGCCGCGCGCCAC
GTCGGCGTGCCGCCCGCGCAGGCGGCCGCCGCGCTGGCCGCGTTCCGCAACGTTAAGCGCCGGATGGAGGTGCGCGGCAG
CGTCGACGGGGTCACCGTCTACGACGATTTCGCTCACCACCCCACCGCGATCGAAACGACGATTGCCGGCCTTCGTACAC
GCATCGGTCGCGAAAACACCCGCATCCTCGCGGTGCTGGAGCCGCGGTCGAACACGATGAAGCTCGGCGTGATGAAGGCG
CAACTGCCGGCGAGCCTGGCCGATGCCGATCTCGTGTTCGGCTACGGCGCGCCGAGCGGCCGCGACGCGCTCGGCTGGAG
CCTGCCCGACGCGCTTGCGCCGCTTGGCGGCAAGGCGCGCGCGTTCGACGATCTGCATGCGCTCGTGAAGGCCGTGACCG
CCTCGGCACGGCCGGGCGACCACGTGCTCGTGATGAGCAACGGCGGCTTCGGCGGTGTGCACCAGAAGCTGCTCGACGCG
CTGTCCGCCCGCGGCGACGCGGCGCCGGCCCGGAGCGGCGCGTGA

Upstream 100 bases:

>100_bases
CGGTGCGGAGCGACCCGGCGGTTGCGTCAAGCGTCGTTCGTGCCGGGGGCGTCAGTTACAATAGCCCTCTTTGCGCGGCG
GCGCGAGCGCCGCGCTGCGC

Downstream 100 bases:

>100_bases
TCCTGTATCTGCATGGCTTCCGTTCGTCGCCGCAGTCGTTCAAGGCGCGCGCGCTGGCCGCGCGGCTAGACGAACTCGGC
CGAGCGGACGAATGGCGCTG

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- me so-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 494; Mature: 494

Protein sequence:

>494_residues
MFSGNRRDVRPAADFDYAHAARSSMHIHILGICGTFMGGLAVLARAAGHTVTGCDAGVYPPMSTQLEAQGIQLIEGYGAE
QIDLKPDLFVIGNVVSRGNPLMEAILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWVLEDAGLNPGFLI
GGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGR
LVTNGRDDALERVLSRGCWSDVERFGVDGGWQALPVENGVPVDGRFAVYWRSERVGAVDWQVQGEHNRMNALAAIAAARH
VGVPPAQAAAALAAFRNVKRRMEVRGSVDGVTVYDDFAHHPTAIETTIAGLRTRIGRENTRILAVLEPRSNTMKLGVMKA
QLPASLADADLVFGYGAPSGRDALGWSLPDALAPLGGKARAFDDLHALVKAVTASARPGDHVLVMSNGGFGGVHQKLLDA
LSARGDAAPARSGA

Sequences:

>Translated_494_residues
MFSGNRRDVRPAADFDYAHAARSSMHIHILGICGTFMGGLAVLARAAGHTVTGCDAGVYPPMSTQLEAQGIQLIEGYGAE
QIDLKPDLFVIGNVVSRGNPLMEAILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWVLEDAGLNPGFLI
GGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGR
LVTNGRDDALERVLSRGCWSDVERFGVDGGWQALPVENGVPVDGRFAVYWRSERVGAVDWQVQGEHNRMNALAAIAAARH
VGVPPAQAAAALAAFRNVKRRMEVRGSVDGVTVYDDFAHHPTAIETTIAGLRTRIGRENTRILAVLEPRSNTMKLGVMKA
QLPASLADADLVFGYGAPSGRDALGWSLPDALAPLGGKARAFDDLHALVKAVTASARPGDHVLVMSNGGFGGVHQKLLDA
LSARGDAAPARSGA
>Mature_494_residues
MFSGNRRDVRPAADFDYAHAARSSMHIHILGICGTFMGGLAVLARAAGHTVTGCDAGVYPPMSTQLEAQGIQLIEGYGAE
QIDLKPDLFVIGNVVSRGNPLMEAILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWVLEDAGLNPGFLI
GGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGR
LVTNGRDDALERVLSRGCWSDVERFGVDGGWQALPVENGVPVDGRFAVYWRSERVGAVDWQVQGEHNRMNALAAIAAARH
VGVPPAQAAAALAAFRNVKRRMEVRGSVDGVTVYDDFAHHPTAIETTIAGLRTRIGRENTRILAVLEPRSNTMKLGVMKA
QLPASLADADLVFGYGAPSGRDALGWSLPDALAPLGGKARAFDDLHALVKAVTASARPGDHVLVMSNGGFGGVHQKLLDA
LSARGDAAPARSGA

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=464, Percent_Identity=58.8362068965517, Blast_Score=543, Evalue=1e-155,
Organism=Escherichia coli, GI1786279, Length=362, Percent_Identity=29.5580110497238, Blast_Score=107, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 52805; Mature: 52805

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSGNRRDVRPAADFDYAHAARSSMHIHILGICGTFMGGLAVLARAAGHTVTGCDAGVYP
CCCCCCCCCCCCCCCCHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCC
PMSTQLEAQGIQLIEGYGAEQIDLKPDLFVIGNVVSRGNPLMEAILDRGLPYVSGPQWLG
CCCCCCHHCCCCEEECCCCCEECCCCCEEEEECHHHCCCHHHHHHHHCCCCCCCCHHHHH
EHVLAGKWVLAVAGTHGKTTTSSMLAWVLEDAGLNPGFLIGGVPLNFGVSARLTDSSFFV
HHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEECCEEEE
IEADEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGR
EECCCCCCHHHHCCCCEEEECCHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCH
LVTNGRDDALERVLSRGCWSDVERFGVDGGWQALPVENGVPVDGRFAVYWRSERVGAVDW
HHCCCCHHHHHHHHHCCCHHHHHHHCCCCCCEEECCCCCCCCCCEEEEEEECCCCCEEEE
QVQGEHNRMNALAAIAAARHVGVPPAQAAAALAAFRNVKRRMEVRGSVDGVTVYDDFAHH
EEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCC
PTAIETTIAGLRTRIGRENTRILAVLEPRSNTMKLGVMKAQLPASLADADLVFGYGAPSG
CCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEHHCCCCCCCCCEEEECCCCCC
RDALGWSLPDALAPLGGKARAFDDLHALVKAVTASARPGDHVLVMSNGGFGGVHQKLLDA
CCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHH
LSARGDAAPARSGA
HHCCCCCCCCCCCC
>Mature Secondary Structure
MFSGNRRDVRPAADFDYAHAARSSMHIHILGICGTFMGGLAVLARAAGHTVTGCDAGVYP
CCCCCCCCCCCCCCCCHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCC
PMSTQLEAQGIQLIEGYGAEQIDLKPDLFVIGNVVSRGNPLMEAILDRGLPYVSGPQWLG
CCCCCCHHCCCCEEECCCCCEECCCCCEEEEECHHHCCCHHHHHHHHCCCCCCCCHHHHH
EHVLAGKWVLAVAGTHGKTTTSSMLAWVLEDAGLNPGFLIGGVPLNFGVSARLTDSSFFV
HHHHCCCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCCCEEEEECCEEEE
IEADEYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHHLVRTVPGVGR
EECCCCCCHHHHCCCCEEEECCHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHCCCCCH
LVTNGRDDALERVLSRGCWSDVERFGVDGGWQALPVENGVPVDGRFAVYWRSERVGAVDW
HHCCCCHHHHHHHHHCCCHHHHHHHCCCCCCEEECCCCCCCCCCEEEEEEECCCCCEEEE
QVQGEHNRMNALAAIAAARHVGVPPAQAAAALAAFRNVKRRMEVRGSVDGVTVYDDFAHH
EEECCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCC
PTAIETTIAGLRTRIGRENTRILAVLEPRSNTMKLGVMKAQLPASLADADLVFGYGAPSG
CCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEEEEHHCCCCCCCCCEEEECCCCCC
RDALGWSLPDALAPLGGKARAFDDLHALVKAVTASARPGDHVLVMSNGGFGGVHQKLLDA
CCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHH
LSARGDAAPARSGA
HHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]