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The map label for this gene is phnE [H]

Identifier: 53720456

GI number: 53720456

Start: 3410457

End: 3411272

Strand: Reverse

Name: phnE [H]

Synonym: BPSL2848

Alternate gene names: 53720456

Gene position: 3411272-3410457 (Counterclockwise)

Preceding gene: 53720457

Following gene: 53720449

Centisome position: 83.72

GC content: 69.73

Gene sequence:

>816_bases
ATGAACGCGCGCGAGCCGGCGGGCGCCGCGCTCGGCGAACCGCTCGCAACGGCCGCCGCCGCGCGCCGCGGCTGGCGGGC
GTGGCTTGCGTGGGCGCTCGCGTTCGCGATTCTCGGCGGCACGTGGCGCAGCGCCGACATGCGGCCGCTCGATCTGCTTG
CCGAGTCCGCGAACATGGGCGAGTTCGCGCGCGGCTTCTTTCCGCCGGACTTCTCCGAATGGCGGTACTACGCGAGCGAG
ATGGGCGTGACGCTGTCGATCGCGCTCTGGGGCACGGCGCTCGCGATCGTGAGCGCCGTGCCGTTCGGCCTGATGTCGGC
GCGCAACATTGCGCCCGCGTGGCTGCTGCATCCGGTGCGGCGCACGATGGACAGCTGCCGTGCGATCAACGAGATGGTGT
TCGCGATGCTGTTCATCGTCGCCGTCGGTCTCGGGCCGTTCTCCGGCGTGCTCGCGCTGTGGGTTCACACGACGGGCGTG
CTCGCGAAGCTTTTCGCGGAGGCGGTGGAGGCGGTCGATCCGCGTCCGGCCGAGGGCGTGCGCGCCGCCGGCGCGACGCC
GCTCGACGAGATCGTCTATGCGGTGCTGCCGCAGGTGCTGCCGTTGTGGATTTCATATGCGCTGTACCGGTTCGAATCGA
ACGTGCGCTCGGCGATGGTGGTCGGGATGGTCGGCGCGGGCGGGATTGGCGTCGTGCTGTACGAGGCGATTCGTTCGTTC
AACTATGCGCAGACGGCCGCGCTGCTCGTCATCGTCATCGCGGTCGTGACGCTGATCGACATGAGCTCCGCGTGGCTGCG
GGCGAGGGCGATCTGA

Upstream 100 bases:

>100_bases
CCGATGCGCGCCGCGCGCAGCTCGCGGCGCTCGATGCGAGGCTCGCCGCGCTCGACGCGTCCGGCGCGGCGGATGCGTCG
AGCGCGCAGGCGGACAAGCG

Downstream 100 bases:

>100_bases
CGCGCGGGGCGGCGCGAGGTGCGCGCGGCGGGCTTGCGATGCGCTCGGGACGTCGAGCGCGCCGCGCGCGCGGCCGCTCG
CGGAGGGAAAGAGAAGCCGG

Product: putative phosphonate transport protein PhnE

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MNAREPAGAALGEPLATAAAARRGWRAWLAWALAFAILGGTWRSADMRPLDLLAESANMGEFARGFFPPDFSEWRYYASE
MGVTLSIALWGTALAIVSAVPFGLMSARNIAPAWLLHPVRRTMDSCRAINEMVFAMLFIVAVGLGPFSGVLALWVHTTGV
LAKLFAEAVEAVDPRPAEGVRAAGATPLDEIVYAVLPQVLPLWISYALYRFESNVRSAMVVGMVGAGGIGVVLYEAIRSF
NYAQTAALLVIVIAVVTLIDMSSAWLRARAI

Sequences:

>Translated_271_residues
MNAREPAGAALGEPLATAAAARRGWRAWLAWALAFAILGGTWRSADMRPLDLLAESANMGEFARGFFPPDFSEWRYYASE
MGVTLSIALWGTALAIVSAVPFGLMSARNIAPAWLLHPVRRTMDSCRAINEMVFAMLFIVAVGLGPFSGVLALWVHTTGV
LAKLFAEAVEAVDPRPAEGVRAAGATPLDEIVYAVLPQVLPLWISYALYRFESNVRSAMVVGMVGAGGIGVVLYEAIRSF
NYAQTAALLVIVIAVVTLIDMSSAWLRARAI
>Mature_271_residues
MNAREPAGAALGEPLATAAAARRGWRAWLAWALAFAILGGTWRSADMRPLDLLAESANMGEFARGFFPPDFSEWRYYASE
MGVTLSIALWGTALAIVSAVPFGLMSARNIAPAWLLHPVRRTMDSCRAINEMVFAMLFIVAVGLGPFSGVLALWVHTTGV
LAKLFAEAVEAVDPRPAEGVRAAGATPLDEIVYAVLPQVLPLWISYALYRFESNVRSAMVVGMVGAGGIGVVLYEAIRSF
NYAQTAALLVIVIAVVTLIDMSSAWLRARAI

Specific function: Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29115; Mature: 29115

Theoretical pI: Translated: 7.51; Mature: 7.51

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAREPAGAALGEPLATAAAARRGWRAWLAWALAFAILGGTWRSADMRPLDLLAESANMG
CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCH
EFARGFFPPDFSEWRYYASEMGVTLSIALWGTALAIVSAVPFGLMSARNIAPAWLLHPVR
HHHHCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RTMDSCRAINEMVFAMLFIVAVGLGPFSGVLALWVHTTGVLAKLFAEAVEAVDPRPAEGV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RAAGATPLDEIVYAVLPQVLPLWISYALYRFESNVRSAMVVGMVGAGGIGVVLYEAIRSF
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
NYAQTAALLVIVIAVVTLIDMSSAWLRARAI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNAREPAGAALGEPLATAAAARRGWRAWLAWALAFAILGGTWRSADMRPLDLLAESANMG
CCCCCCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCH
EFARGFFPPDFSEWRYYASEMGVTLSIALWGTALAIVSAVPFGLMSARNIAPAWLLHPVR
HHHHCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
RTMDSCRAINEMVFAMLFIVAVGLGPFSGVLALWVHTTGVLAKLFAEAVEAVDPRPAEGV
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
RAAGATPLDEIVYAVLPQVLPLWISYALYRFESNVRSAMVVGMVGAGGIGVVLYEAIRSF
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
NYAQTAALLVIVIAVVTLIDMSSAWLRARAI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]