Click here to switch to the map view.

The map label for this gene is waaA [H]

Identifier: 53720273

GI number: 53720273

Start: 3185671

End: 3187056

Strand: Reverse

Name: waaA [H]

Synonym: BPSL2663

Alternate gene names: 53720273

Gene position: 3187056-3185671 (Counterclockwise)

Preceding gene: 53720274

Following gene: 53720260

Centisome position: 78.22

GC content: 74.6

Gene sequence:

>1386_bases
ATGCTGAGGGCGATCTATCGCGGACTGTGGTGGCTCGTCGCGCCGCTCGCGGTGCTGCGCCTCGTGTGGCGCTCGCGCAA
GGAGCGCGGCTATCGCGAGCATATCGGCGAGCGCTTCGGCTTCGGGCCGGGGCGCGCGCTCGCGCGCGACGTCGACGAAG
CGACGCCGATCGTGTGGGTGCATGCGGTGTCGGTCGGCGAGACGCGCGCCGCGCAGCCGCTCATCGATGCGCTGTTGCGC
GCGCGCCCCGACGCGCACGTGCTGCTCACGCACATGACGCCGAGCGGCCGCGCGACGGGCGAGCAGGTCTTCGGCGAGCG
CGTGTCGCGCTGCTACCTGCCGTACGATCTGCCGCGCGCCGTGCGGCGCTTCCTGCGCGCATGGCGGCCGTCGCTCGGGC
TCGTGATGGAAACCGAGGTCTGGCCGACGCTTATTGACGAATGCCGCCGCGCCGACGTGCCGCTCGTGCTGACGAATGCG
CGGATGTCGGCGCGCTCGTTCGGGCGCGCAGCGAAATTCGGCGCCGCCGCGCGCGACGTGTTCGGCGGCTTCTCGCGCGT
GCTCGCGCAAAGCCCCGCCGACGCGCAGCGCCTGACTTCGCTCGGCGCGCGCAACGTCGCGGTGCTCGGCAATCTGAAGT
TCGACATGACGACACCGCCCGAGCTCGCCGCGCGCGGGCGCGCATGGCGCGCGGCGATCGGCGAGCGGCCCGTGTGGGTG
GCCGCGAGCACGCGCGACGGCGAAGAGGCGCTCGTGCTCGACGCATTCGTCTCGCTGAAGACGCCGGACGCGTTGCTGAT
CCTCGTGCCGCGTCATCCGCAGCGCTTCGCCGAAGTCGCGGCGCTCGTCGAGCGGCGCGGGCTGCGCCACGTGCGCCGCA
CCGAATGGGCGCACGACGCGGCGGCCGCCGCCGCGGGCCGGCCGGCCGCCTCCGCGCTGCCGGCCGGCGTCTCGGTGCTG
CTCGGCGATTCGATGGGCGAACTGGGCGCGTACTATGCGGCCGCCGACGTCGCGTTCATCGGCGGCAGCCTGTTGCCGCT
CGGCGGCCAGAACCTGATCGAGGCGTGCGCGGTCGGCGTGCCGGTGCTGATCGGGCCGCACGTGTTCAACTTCACGCAGG
CGACGGCCGATGCGGTCACGGCGGGCGCGTGCGCGCAAGTGCGGGATCCGGCCGATCTCGCATGCACGCTCGACGCGCTG
TTCGCCGATCACGCGCGCCGCACGGCGATGGGCGCGGCGGGCGCGGCGTTCGCCGCGCGGCATCGCGGCGCGACGGCGCG
CACGGTCGACGTGCTGAACGCGCTGCTGCCGGCGCCGCGCGTCGCGCCGGCGGCGAACGACGAAGGCGATGCGGGCGGGC
TCGATGCGGGCGCGGCGCTCGACTGA

Upstream 100 bases:

>100_bases
GCCCGCCCGCAGCGCGCGCCGCTCGGCATCCTCGAGCGTCTGCAGGGCAGGGCCCTCGTTTGAGCGCGCGCGACGCAGTC
GCGGCCGGAGCCGCCGCGCG

Downstream 100 bases:

>100_bases
GCGGTGCGGGCGGCGTTGCGTGCGCGTGGCTGAGCGAGCATGGGCGGGGGCGTTGCCGCATCGGCTGCCGCGTTGCGCGG
CGTGCCGTTTCGGGAGCCGC

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: KDO2-lipid IVA; CMP; KDO1-lipid IVA [C]

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MLRAIYRGLWWLVAPLAVLRLVWRSRKERGYREHIGERFGFGPGRALARDVDEATPIVWVHAVSVGETRAAQPLIDALLR
ARPDAHVLLTHMTPSGRATGEQVFGERVSRCYLPYDLPRAVRRFLRAWRPSLGLVMETEVWPTLIDECRRADVPLVLTNA
RMSARSFGRAAKFGAAARDVFGGFSRVLAQSPADAQRLTSLGARNVAVLGNLKFDMTTPPELAARGRAWRAAIGERPVWV
AASTRDGEEALVLDAFVSLKTPDALLILVPRHPQRFAEVAALVERRGLRHVRRTEWAHDAAAAAAGRPAASALPAGVSVL
LGDSMGELGAYYAAADVAFIGGSLLPLGGQNLIEACAVGVPVLIGPHVFNFTQATADAVTAGACAQVRDPADLACTLDAL
FADHARRTAMGAAGAAFAARHRGATARTVDVLNALLPAPRVAPAANDEGDAGGLDAGAALD

Sequences:

>Translated_461_residues
MLRAIYRGLWWLVAPLAVLRLVWRSRKERGYREHIGERFGFGPGRALARDVDEATPIVWVHAVSVGETRAAQPLIDALLR
ARPDAHVLLTHMTPSGRATGEQVFGERVSRCYLPYDLPRAVRRFLRAWRPSLGLVMETEVWPTLIDECRRADVPLVLTNA
RMSARSFGRAAKFGAAARDVFGGFSRVLAQSPADAQRLTSLGARNVAVLGNLKFDMTTPPELAARGRAWRAAIGERPVWV
AASTRDGEEALVLDAFVSLKTPDALLILVPRHPQRFAEVAALVERRGLRHVRRTEWAHDAAAAAAGRPAASALPAGVSVL
LGDSMGELGAYYAAADVAFIGGSLLPLGGQNLIEACAVGVPVLIGPHVFNFTQATADAVTAGACAQVRDPADLACTLDAL
FADHARRTAMGAAGAAFAARHRGATARTVDVLNALLPAPRVAPAANDEGDAGGLDAGAALD
>Mature_461_residues
MLRAIYRGLWWLVAPLAVLRLVWRSRKERGYREHIGERFGFGPGRALARDVDEATPIVWVHAVSVGETRAAQPLIDALLR
ARPDAHVLLTHMTPSGRATGEQVFGERVSRCYLPYDLPRAVRRFLRAWRPSLGLVMETEVWPTLIDECRRADVPLVLTNA
RMSARSFGRAAKFGAAARDVFGGFSRVLAQSPADAQRLTSLGARNVAVLGNLKFDMTTPPELAARGRAWRAAIGERPVWV
AASTRDGEEALVLDAFVSLKTPDALLILVPRHPQRFAEVAALVERRGLRHVRRTEWAHDAAAAAAGRPAASALPAGVSVL
LGDSMGELGAYYAAADVAFIGGSLLPLGGQNLIEACAVGVPVLIGPHVFNFTQATADAVTAGACAQVRDPADLACTLDAL
FADHARRTAMGAAGAAFAARHRGATARTVDVLNALLPAPRVAPAANDEGDAGGLDAGAALD

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=447, Percent_Identity=40.0447427293065, Blast_Score=298, Evalue=4e-82,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 49098; Mature: 49098

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRAIYRGLWWLVAPLAVLRLVWRSRKERGYREHIGERFGFGPGRALARDVDEATPIVWV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEE
HAVSVGETRAAQPLIDALLRARPDAHVLLTHMTPSGRATGEQVFGERVSRCYLPYDLPRA
EEEECCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHH
VRRFLRAWRPSLGLVMETEVWPTLIDECRRADVPLVLTNARMSARSFGRAAKFGAAARDV
HHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
FGGFSRVLAQSPADAQRLTSLGARNVAVLGNLKFDMTTPPELAARGRAWRAAIGERPVWV
HHHHHHHHHCCCCHHHHHHHCCCCCEEEEECEEECCCCCHHHHHCCCHHHHHHCCCCEEE
AASTRDGEEALVLDAFVSLKTPDALLILVPRHPQRFAEVAALVERRGLRHVRRTEWAHDA
EECCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAAAGRPAASALPAGVSVLLGDSMGELGAYYAAADVAFIGGSLLPLGGQNLIEACAVGV
HHHHCCCCCHHHCCCCHHEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
PVLIGPHVFNFTQATADAVTAGACAQVRDPADLACTLDALFADHARRTAMGAAGAAFAAR
CEEECCCHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
HRGATARTVDVLNALLPAPRVAPAANDEGDAGGLDAGAALD
HCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLRAIYRGLWWLVAPLAVLRLVWRSRKERGYREHIGERFGFGPGRALARDVDEATPIVWV
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEE
HAVSVGETRAAQPLIDALLRARPDAHVLLTHMTPSGRATGEQVFGERVSRCYLPYDLPRA
EEEECCCHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCHHHH
VRRFLRAWRPSLGLVMETEVWPTLIDECRRADVPLVLTNARMSARSFGRAAKFGAAARDV
HHHHHHHHCCCCCEEEECHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHH
FGGFSRVLAQSPADAQRLTSLGARNVAVLGNLKFDMTTPPELAARGRAWRAAIGERPVWV
HHHHHHHHHCCCCHHHHHHHCCCCCEEEEECEEECCCCCHHHHHCCCHHHHHHCCCCEEE
AASTRDGEEALVLDAFVSLKTPDALLILVPRHPQRFAEVAALVERRGLRHVRRTEWAHDA
EECCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AAAAAGRPAASALPAGVSVLLGDSMGELGAYYAAADVAFIGGSLLPLGGQNLIEACAVGV
HHHHCCCCCHHHCCCCHHEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCC
PVLIGPHVFNFTQATADAVTAGACAQVRDPADLACTLDALFADHARRTAMGAAGAAFAAR
CEEECCCHHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
HRGATARTVDVLNALLPAPRVAPAANDEGDAGGLDAGAALD
HCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: KDO1-lipid IVA; CMP-3-deoxy-D-manno-octulosonate; lipid IVA [C]

Specific reaction: KDO1-lipid IVA + CMP-3-deoxy-D-manno-octulosonate = KDO2-lipid IVA + CMP CMP-3-deoxy-D-manno-octulosonate + lipid IVA = KDO1-lipid IVA + CMP [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]