Click here to switch to the map view.

The map label for this gene is gph [C]

Identifier: 53720056

GI number: 53720056

Start: 2953348

End: 2954007

Strand: Reverse

Name: gph [C]

Synonym: BPSL2450

Alternate gene names: 53720056

Gene position: 2954007-2953348 (Counterclockwise)

Preceding gene: 53720057

Following gene: 53720055

Centisome position: 72.5

GC content: 70.3

Gene sequence:

>660_bases
ATGGCCCGACAGCAATTTGATCTGATCGTCTTCGACTGGGACGGCACGCTGATGGATTCGACCGCGCACATCGCGCAGTG
CATCCAGGCCGCGTGCCGCGATCTCGGCGTGCCCGCGCCGTCCGACGAGGCCGCCCGCTACGTGATCGGGCTCGGCCTGC
GCGACGCGCTGGCCGTGACCGCGCCGAGCGTCGATCCCGCCGATTATCCGCGGCTCGCCGAGCGCTACCGGTTTCACTAT
CTGGTCAAGGATCAGCGAACCGAGCTCTTCGGCGGCGTGCGCGAGATGCTGGAAGAGCTGCGCGACACGGGCTACCTGCT
GGCCGTCGCGACGGGCAAGGGGCGCGTCGGGCTGAATCGCGCGCTCGATCAGGCGAAGCTCACGAGCCTCTTCGACGCCA
CGCGCTGCGCGGACGAGACATTCTCGAAGCCGCATCCGGCGATGCTGCAGGAATTGTCGCGCGAATTGGGGCAGGATCTG
GCCCGCACCGTGATGATCGGCGACACCACGCACGATCTGCAGATGGCGGCGAGCGCGGGCGCCGCGGGCGTCGGCGTCGC
GTACGGCGCGCATTCGGCCGACGCGCTCGCGGCGCTGTCGCCGCGCTTCGTCGCGGCCGACGTCGCGGCGCTCGCCGGCT
GGCTGCGGGAGCACGCATGA

Upstream 100 bases:

>100_bases
CGCTGACGGGCGAGCCGCTGCAGTTCGACGCGCCGCTGCCCGCCGAGTGCCGGCAATTCATCGATCAACTCTCCGACTTG
CGCGACACCGCGTGACCAGC

Downstream 100 bases:

>100_bases
CGGGCGATGGCGACGCGCGCTTCGTGTGCGCGGCCGACGCGCTCGTCGACGGCGGCGAGGGCGTGCGGCTCGACGCGACG
CTGCGCGGCGAGCCCGCCGT

Product: haloacid dehalogenase-like hydrolase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MARQQFDLIVFDWDGTLMDSTAHIAQCIQAACRDLGVPAPSDEAARYVIGLGLRDALAVTAPSVDPADYPRLAERYRFHY
LVKDQRTELFGGVREMLEELRDTGYLLAVATGKGRVGLNRALDQAKLTSLFDATRCADETFSKPHPAMLQELSRELGQDL
ARTVMIGDTTHDLQMAASAGAAGVGVAYGAHSADALAALSPRFVAADVAALAGWLREHA

Sequences:

>Translated_219_residues
MARQQFDLIVFDWDGTLMDSTAHIAQCIQAACRDLGVPAPSDEAARYVIGLGLRDALAVTAPSVDPADYPRLAERYRFHY
LVKDQRTELFGGVREMLEELRDTGYLLAVATGKGRVGLNRALDQAKLTSLFDATRCADETFSKPHPAMLQELSRELGQDL
ARTVMIGDTTHDLQMAASAGAAGVGVAYGAHSADALAALSPRFVAADVAALAGWLREHA
>Mature_218_residues
ARQQFDLIVFDWDGTLMDSTAHIAQCIQAACRDLGVPAPSDEAARYVIGLGLRDALAVTAPSVDPADYPRLAERYRFHYL
VKDQRTELFGGVREMLEELRDTGYLLAVATGKGRVGLNRALDQAKLTSLFDATRCADETFSKPHPAMLQELSRELGQDLA
RTVMIGDTTHDLQMAASAGAAGVGVAYGAHSADALAALSPRFVAADVAALAGWLREHA

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=227, Percent_Identity=25.9911894273128, Blast_Score=60, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR000150
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23573; Mature: 23442

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARQQFDLIVFDWDGTLMDSTAHIAQCIQAACRDLGVPAPSDEAARYVIGLGLRDALAVT
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHEE
APSVDPADYPRLAERYRFHYLVKDQRTELFGGVREMLEELRDTGYLLAVATGKGRVGLNR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHH
ALDQAKLTSLFDATRCADETFSKPHPAMLQELSRELGQDLARTVMIGDTTHDLQMAASAG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
AAGVGVAYGAHSADALAALSPRFVAADVAALAGWLREHA
CCCCHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ARQQFDLIVFDWDGTLMDSTAHIAQCIQAACRDLGVPAPSDEAARYVIGLGLRDALAVT
CCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCHHHHHHEE
APSVDPADYPRLAERYRFHYLVKDQRTELFGGVREMLEELRDTGYLLAVATGKGRVGLNR
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHH
ALDQAKLTSLFDATRCADETFSKPHPAMLQELSRELGQDLARTVMIGDTTHDLQMAASAG
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCC
AAGVGVAYGAHSADALAALSPRFVAADVAALAGWLREHA
CCCCHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA