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The map label for this gene is puuA [H]

Identifier: 53719946

GI number: 53719946

Start: 2822562

End: 2823896

Strand: Reverse

Name: puuA [H]

Synonym: BPSL2336

Alternate gene names: 53719946

Gene position: 2823896-2822562 (Counterclockwise)

Preceding gene: 53719947

Following gene: 53719945

Centisome position: 69.31

GC content: 64.27

Gene sequence:

>1335_bases
ATGCAAGACATCGATGATTTTTTGAGGCAGCACCGGATCACCGAAGTGGAAGCGATCATTCCCGACATGGCGGGCATCGC
GCGCGGCAAGATCATTCCGCGCAACAAGTTCGAAACCGGCGAATCGATGCGCCTGCCGCAAGCGGTGATGGTGCAGACCG
TCACCGGCGATTATCCGGAGGACGGCACGCTCACGGGCGTCACGGATCCCGACATGGTGTGCGTGGCCGACGCGTCGACG
ATCTGCCTGATTCCGTGGGCAGTCGACCCCACCGCGCAGGTGATTCACGATTGCGTGCATTTCGACGGCTCGCCCGTCGA
AATTTCGCCGCGCTACGTGCTGCGCCGCGTGCTCGACCTGTATCGCGCGAGGGGGTGGAAGCCCGTCGTCGCGCCGGAGC
TCGAGTTCTATCTGGTCGACATGAACAAGGACCCGGACCTGCCGCTGCGGCCGCCCGTCGGACGCACGGGGCGCGCGGAA
ACGGGGCGGCAGTCGTACTCGATCGAAGCGGTCAACGAATTCGATCCGCTCTTCGAGGACATCTACGAGTACTGCGAGCT
GCAGGGCCTCGACATCGACACGCTGATTCACGAAGTCGGCGCCGCGCAGATGGAGATCAACTTCCTGCACGGCGATGCGC
TATCGCTCGCCGACCAGGTGTTTCTGTTCAAGCGCACGGTGCGCGAGGCGGCGCTGCGCCACAACATGTACGCCACCTTC
ATGGCAAAGCCGATGGAGAGCGAGCCCGGCTCGGCGATGCACATTCATCAGAGCGTCGTCGACATCGAGACCGGGCGCAA
TCTCTTCATCGGCGCGAGCGGCGACGTGACGCCGATGTTCCGCAGCTATCTCGCCGGGCTGCAGAAGTACACGCCCGCGC
TGATGCCGATCTTCGCGCCGTACATCAACTCGTACCGGCGGCTGTCGCGCTTCATGGCCGCGCCGATCAACGTGCAATGG
GGCTACGACAACCGCACGGTCGGCTTCCGGATTCCGCACTCGGCGGCCGCCGCGCGGCGGATCGAGAACCGGATTCCGGG
CGTCGACTGCAACCCGTATCTCGCGATCGCGGGCACGCTCGCGGCGGGCTACCTCGGCGTCACGCAGGGGCTCGAGCCGA
CCGAGCCGCTCGCGAGCGACGGCTACAGCCTGCCGTATCAACTGCCGCGCAATCTGGAGGAAGGGCTCACGCTGATGAGC
GCATGCGCGCCGCTTGCCGAGATGCTCGGCGAGAAATTCGTGAAAGCCTATCTCGCATTGAAGGAAACGGAGTACGAAGC
ATTTTTCCGCGTGATCAGCTCGTGGGAGCGCAGGCACCTGCTGCTGCACGTTTGA

Upstream 100 bases:

>100_bases
TTGCGGCGTTCGGCGCGGCGTGCCGCGCACGAAGACGCGCGACGGCCGGGCGCGCGCCGCGCGCGTGACGGCCGTCGACG
CTTATCGAAGAGAGGCTCAC

Downstream 100 bases:

>100_bases
GCGTGCGCACGCACGCCGCATTCAGGAGATCCAAGTGACGTATCGAACCGAAGACATCGCCGATGTAGCGCCGCTGTACG
CGAGCGCCGCGCCCGCGCAC

Product: putative glutamine synthetase

Products: NA

Alternate protein names: Gamma-Glu-Put synthetase; Glutamate--putrescine ligase [H]

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MQDIDDFLRQHRITEVEAIIPDMAGIARGKIIPRNKFETGESMRLPQAVMVQTVTGDYPEDGTLTGVTDPDMVCVADAST
ICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYRARGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRAE
TGRQSYSIEAVNEFDPLFEDIYEYCELQGLDIDTLIHEVGAAQMEINFLHGDALSLADQVFLFKRTVREAALRHNMYATF
MAKPMESEPGSAMHIHQSVVDIETGRNLFIGASGDVTPMFRSYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQW
GYDNRTVGFRIPHSAAAARRIENRIPGVDCNPYLAIAGTLAAGYLGVTQGLEPTEPLASDGYSLPYQLPRNLEEGLTLMS
ACAPLAEMLGEKFVKAYLALKETEYEAFFRVISSWERRHLLLHV

Sequences:

>Translated_444_residues
MQDIDDFLRQHRITEVEAIIPDMAGIARGKIIPRNKFETGESMRLPQAVMVQTVTGDYPEDGTLTGVTDPDMVCVADAST
ICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYRARGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRAE
TGRQSYSIEAVNEFDPLFEDIYEYCELQGLDIDTLIHEVGAAQMEINFLHGDALSLADQVFLFKRTVREAALRHNMYATF
MAKPMESEPGSAMHIHQSVVDIETGRNLFIGASGDVTPMFRSYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQW
GYDNRTVGFRIPHSAAAARRIENRIPGVDCNPYLAIAGTLAAGYLGVTQGLEPTEPLASDGYSLPYQLPRNLEEGLTLMS
ACAPLAEMLGEKFVKAYLALKETEYEAFFRVISSWERRHLLLHV
>Mature_444_residues
MQDIDDFLRQHRITEVEAIIPDMAGIARGKIIPRNKFETGESMRLPQAVMVQTVTGDYPEDGTLTGVTDPDMVCVADAST
ICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDLYRARGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRAE
TGRQSYSIEAVNEFDPLFEDIYEYCELQGLDIDTLIHEVGAAQMEINFLHGDALSLADQVFLFKRTVREAALRHNMYATF
MAKPMESEPGSAMHIHQSVVDIETGRNLFIGASGDVTPMFRSYLAGLQKYTPALMPIFAPYINSYRRLSRFMAAPINVQW
GYDNRTVGFRIPHSAAAARRIENRIPGVDCNPYLAIAGTLAAGYLGVTQGLEPTEPLASDGYSLPYQLPRNLEEGLTLMS
ACAPLAEMLGEKFVKAYLALKETEYEAFFRVISSWERRHLLLHV

Specific function: Involved in the breakdown of putrescine via the biosynthesis of gamma-L-glutamylputrescine [H]

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=443, Percent_Identity=26.86230248307, Blast_Score=153, Evalue=2e-37,
Organism=Escherichia coli, GI87081870, Length=450, Percent_Identity=35.3333333333333, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1790301, Length=463, Percent_Identity=28.0777537796976, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146 [H]

Pfam domain/function: PF00120 Gln-synt_C [H]

EC number: =6.3.1.11 [H]

Molecular weight: Translated: 49735; Mature: 49735

Theoretical pI: Translated: 4.93; Mature: 4.93

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDIDDFLRQHRITEVEAIIPDMAGIARGKIIPRNKFETGESMRLPQAVMVQTVTGDYPE
CCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHEEECCCCCCC
DGTLTGVTDPDMVCVADASTICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDL
CCEEECCCCCCEEEEECCCEEEEEEECCCHHHHHHHHHHHCCCCCEEECHHHHHHHHHHH
YRARGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRAETGRQSYSIEAVNEFDPLFED
HHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHH
IYEYCELQGLDIDTLIHEVGAAQMEINFLHGDALSLADQVFLFKRTVREAALRHNMYATF
HHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAKPMESEPGSAMHIHQSVVDIETGRNLFIGASGDVTPMFRSYLAGLQKYTPALMPIFAP
HCCCCCCCCCCEEEHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHH
YINSYRRLSRFMAAPINVQWGYDNRTVGFRIPHSAAAARRIENRIPGVDCNPYLAIAGTL
HHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
AAGYLGVTQGLEPTEPLASDGYSLPYQLPRNLEEGLTLMSACAPLAEMLGEKFVKAYLAL
HHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KETEYEAFFRVISSWERRHLLLHV
HHHHHHHHHHHHHHHHHCEEEEEC
>Mature Secondary Structure
MQDIDDFLRQHRITEVEAIIPDMAGIARGKIIPRNKFETGESMRLPQAVMVQTVTGDYPE
CCCHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHEEECCCCCCC
DGTLTGVTDPDMVCVADASTICLIPWAVDPTAQVIHDCVHFDGSPVEISPRYVLRRVLDL
CCEEECCCCCCEEEEECCCEEEEEEECCCHHHHHHHHHHHCCCCCEEECHHHHHHHHHHH
YRARGWKPVVAPELEFYLVDMNKDPDLPLRPPVGRTGRAETGRQSYSIEAVNEFDPLFED
HHHCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHH
IYEYCELQGLDIDTLIHEVGAAQMEINFLHGDALSLADQVFLFKRTVREAALRHNMYATF
HHHHHHCCCCCHHHHHHHHCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MAKPMESEPGSAMHIHQSVVDIETGRNLFIGASGDVTPMFRSYLAGLQKYTPALMPIFAP
HCCCCCCCCCCEEEHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHHCHHHHHHHHH
YINSYRRLSRFMAAPINVQWGYDNRTVGFRIPHSAAAARRIENRIPGVDCNPYLAIAGTL
HHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCHHHHHHHHHHCCCCCCCCCHHHHHHHH
AAGYLGVTQGLEPTEPLASDGYSLPYQLPRNLEEGLTLMSACAPLAEMLGEKFVKAYLAL
HHHHHHHHCCCCCCCCHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KETEYEAFFRVISSWERRHLLLHV
HHHHHHHHHHHHHHHHHCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]