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The map label for this gene is shiA [H]

Identifier: 53719937

GI number: 53719937

Start: 2812366

End: 2813709

Strand: Reverse

Name: shiA [H]

Synonym: BPSL2327

Alternate gene names: 53719937

Gene position: 2813709-2812366 (Counterclockwise)

Preceding gene: 53719938

Following gene: 53719934

Centisome position: 69.06

GC content: 71.21

Gene sequence:

>1344_bases
ATGGAGACAACGATGACATTCCAGCACGACGAGCGCGCCGAGCGCGCGCCGGTCGGCCAGCCCGCGCGCGCCGCGCTCGC
GGCATTCGTCGGCACGACGATCGAGTGGTACGACTTCTATCTTTACGCCACCGCGTCCGCGCTCATCTTCGGCAAGCTGT
TCTTTCCGTCGGGCGATCCGTTCTTCAGCACGCTCGCGTCGTTCGGCACGTTCGCGGTCGGCTTTTTCGCGCGGCCGTTC
GGCGGCCTCGTGTTCGGCCACCTCGGCGACCGGGTCGGCCGCAAGAAGGCGCTCGTCGCGACGCTCGTCATCATGGCGGT
CGGCACGGTCGGCATCGGCCTGCTGCCCACGTACGCGCAGGCGGGCGCCGTCGCGCCCGTCGCGCTCGTCGTGCTGCGCA
TCGCGCAAGGCATCGCGATCGGCGGCGAGTGGGGCGGCGCGGTGCTGATGGCGAGCGAGCACGCGAGCGCGGACAGGCGC
ACGTTCGTCGCGTCGTTCGCGCAGCTCGGCAGCCCCGCCGGGTTGATTCTCGCGCTACTCGCGTTTCGCCTCGTCGCCGG
CATCGAGCATGACGCGTTCCTCGCGTGGGGCTGGCGGCTGCCGTTTCTCGCGAGCGCGGCGTTGCTCGTGGTGGGGCTCG
CGATCCGCGCGGGCGTCGACGAATCGCCGGAGTTCGCGCGCATGAAGGCGCAGCGGCGCACGGCGGCGCTGCCCGTGGCC
GACGTGGTGCGCGACGCCTGGCGCACGCTGTTGCTCTGCCTCGGCGCGAACGTGATCGGCGTCGCGGGCGCGTGGTTCGT
CAACACGTTCATGCTGAACTACACGACGCAGACGCTCGGGCTCGATCGCGCGCTGATTCTCGACTGCCTGTTCGTCGTCG
CGTTCATCCAGCTCGGCACGCAGCTCGCGTCCGGCTGGTTCGCGCAGCGCATCGGCACCGGGCGCTTCCTGACGGCGGCC
GCCGCGCTCGCGATCGCGTCGCCGTATCCGATGTTCGCGCTCGTGTCGACGGGGCGCCCCGTGGCGATCGTCGTCGGCAT
CGCGATCGCGGTGATGTGCATGTCGAGCTCGTACGCGGTGATGGCGGGCTTCATGTCGACCGCGTTCGACGTGCGCGTGC
GCTACTCGGCGATCTCGCTGTCGTACCAGCTCTGCGCGGCGCTCGCGGGCGGGCTCACGCCGCTCGCGGGCACGCTGCTC
GCGCACCGTTTCGCCGGCCAGTGGTGGCCGCTCGCCGCGTTCTACAGCGCGCTCGCGGTGGTGTCGCTCGCCTGCATCGC
GACACTCGAGCGCGGCAAGCGCGTCGGCGTTGGCGAACGGCGCGGAGCGCTCGCGAAGCCTTGA

Upstream 100 bases:

>100_bases
GTCTTCGGCCGAACGGTTCGCGCCTGCCGGCGCGACCGAATCCGGAGCGATCCGGCCCGACCGGTGAAGGCGCGCGCGAG
TCGCGCCGCCGGCAACAGAC

Downstream 100 bases:

>100_bases
CGGCGCCGCGCGGGCCGGCGCGCGCCGCCCTCGGCAAGCGGGTGCGCCGCGTGCCGAGGGCGAGGCGCCCGTAAAAAAAG
CCTCGGGCAGCAATCGCTGC

Product: putative transporter protein

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
METTMTFQHDERAERAPVGQPARAALAAFVGTTIEWYDFYLYATASALIFGKLFFPSGDPFFSTLASFGTFAVGFFARPF
GGLVFGHLGDRVGRKKALVATLVIMAVGTVGIGLLPTYAQAGAVAPVALVVLRIAQGIAIGGEWGGAVLMASEHASADRR
TFVASFAQLGSPAGLILALLAFRLVAGIEHDAFLAWGWRLPFLASAALLVVGLAIRAGVDESPEFARMKAQRRTAALPVA
DVVRDAWRTLLLCLGANVIGVAGAWFVNTFMLNYTTQTLGLDRALILDCLFVVAFIQLGTQLASGWFAQRIGTGRFLTAA
AALAIASPYPMFALVSTGRPVAIVVGIAIAVMCMSSSYAVMAGFMSTAFDVRVRYSAISLSYQLCAALAGGLTPLAGTLL
AHRFAGQWWPLAAFYSALAVVSLACIATLERGKRVGVGERRGALAKP

Sequences:

>Translated_447_residues
METTMTFQHDERAERAPVGQPARAALAAFVGTTIEWYDFYLYATASALIFGKLFFPSGDPFFSTLASFGTFAVGFFARPF
GGLVFGHLGDRVGRKKALVATLVIMAVGTVGIGLLPTYAQAGAVAPVALVVLRIAQGIAIGGEWGGAVLMASEHASADRR
TFVASFAQLGSPAGLILALLAFRLVAGIEHDAFLAWGWRLPFLASAALLVVGLAIRAGVDESPEFARMKAQRRTAALPVA
DVVRDAWRTLLLCLGANVIGVAGAWFVNTFMLNYTTQTLGLDRALILDCLFVVAFIQLGTQLASGWFAQRIGTGRFLTAA
AALAIASPYPMFALVSTGRPVAIVVGIAIAVMCMSSSYAVMAGFMSTAFDVRVRYSAISLSYQLCAALAGGLTPLAGTLL
AHRFAGQWWPLAAFYSALAVVSLACIATLERGKRVGVGERRGALAKP
>Mature_447_residues
METTMTFQHDERAERAPVGQPARAALAAFVGTTIEWYDFYLYATASALIFGKLFFPSGDPFFSTLASFGTFAVGFFARPF
GGLVFGHLGDRVGRKKALVATLVIMAVGTVGIGLLPTYAQAGAVAPVALVVLRIAQGIAIGGEWGGAVLMASEHASADRR
TFVASFAQLGSPAGLILALLAFRLVAGIEHDAFLAWGWRLPFLASAALLVVGLAIRAGVDESPEFARMKAQRRTAALPVA
DVVRDAWRTLLLCLGANVIGVAGAWFVNTFMLNYTTQTLGLDRALILDCLFVVAFIQLGTQLASGWFAQRIGTGRFLTAA
AALAIASPYPMFALVSTGRPVAIVVGIAIAVMCMSSSYAVMAGFMSTAFDVRVRYSAISLSYQLCAALAGGLTPLAGTLL
AHRFAGQWWPLAAFYSALAVVSLACIATLERGKRVGVGERRGALAKP

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=416, Percent_Identity=35.0961538461538, Blast_Score=248, Evalue=5e-67,
Organism=Escherichia coli, GI1789941, Length=401, Percent_Identity=34.9127182044888, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1790550, Length=423, Percent_Identity=30.2600472813239, Blast_Score=201, Evalue=8e-53,
Organism=Escherichia coli, GI1788942, Length=396, Percent_Identity=31.5656565656566, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI87082231, Length=226, Percent_Identity=25.2212389380531, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 47117; Mature: 47117

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METTMTFQHDERAERAPVGQPARAALAAFVGTTIEWYDFYLYATASALIFGKLFFPSGDP
CCCCEECCCCCHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCH
FFSTLASFGTFAVGFFARPFGGLVFGHLGDRVGRKKALVATLVIMAVGTVGIGLLPTYAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGAVAPVALVVLRIAQGIAIGGEWGGAVLMASEHASADRRTFVASFAQLGSPAGLILALL
CCCHHHHHHHHHHHHCCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHH
AFRLVAGIEHDAFLAWGWRLPFLASAALLVVGLAIRAGVDESPEFARMKAQRRTAALPVA
HHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHH
DVVRDAWRTLLLCLGANVIGVAGAWFVNTFMLNYTTQTLGLDRALILDCLFVVAFIQLGT
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QLASGWFAQRIGTGRFLTAAAALAIASPYPMFALVSTGRPVAIVVGIAIAVMCMSSSYAV
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHH
MAGFMSTAFDVRVRYSAISLSYQLCAALAGGLTPLAGTLLAHRFAGQWWPLAAFYSALAV
HHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VSLACIATLERGKRVGVGERRGALAKP
HHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
METTMTFQHDERAERAPVGQPARAALAAFVGTTIEWYDFYLYATASALIFGKLFFPSGDP
CCCCEECCCCCHHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCH
FFSTLASFGTFAVGFFARPFGGLVFGHLGDRVGRKKALVATLVIMAVGTVGIGLLPTYAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGAVAPVALVVLRIAQGIAIGGEWGGAVLMASEHASADRRTFVASFAQLGSPAGLILALL
CCCHHHHHHHHHHHHCCCEECCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHH
AFRLVAGIEHDAFLAWGWRLPFLASAALLVVGLAIRAGVDESPEFARMKAQRRTAALPVA
HHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHH
DVVRDAWRTLLLCLGANVIGVAGAWFVNTFMLNYTTQTLGLDRALILDCLFVVAFIQLGT
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
QLASGWFAQRIGTGRFLTAAAALAIASPYPMFALVSTGRPVAIVVGIAIAVMCMSSSYAV
HHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCHHH
MAGFMSTAFDVRVRYSAISLSYQLCAALAGGLTPLAGTLLAHRFAGQWWPLAAFYSALAV
HHHHHHHHHHHEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHH
VSLACIATLERGKRVGVGERRGALAKP
HHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]