Click here to switch to the map view.

The map label for this gene is pdhB [H]

Identifier: 53719910

GI number: 53719910

Start: 2768796

End: 2770436

Strand: Reverse

Name: pdhB [H]

Synonym: BPSL2300

Alternate gene names: 53719910

Gene position: 2770436-2768796 (Counterclockwise)

Preceding gene: 53719911

Following gene: 53719909

Centisome position: 67.99

GC content: 69.23

Gene sequence:

>1641_bases
ATGAGTCAAGCGATCGAAGTCAAGGTGCCGGATATCGGCGATTACAAGGACGTGCCCGTCATCGAAGTGCTCGTGAAGCC
GGGCGATGCGGTCGAGCCCGAGCAGTCGCTCGTCACGCTCGAATCGGACAAGGCGACGATGGACGTGCCGAGCCCGTCGG
CGGGCACGGTCAAGGAAGTGAAGGTGAAGGTCGGCGACGCGGTGTCGCAAGGCTCGCTGATCGTGCTGCTCGACGGCGCG
CAGGCGGCGGCCCAGCCCGCGCAGGCGAACGGCGCCGCGACGAGCGCCGCGCAGCCGGCGGCGGCGCCCGCTGCCGCGCC
TGCGCCGGCGGCGGCCGCGGGCGGCGGCACGGTCGACGTGAAGGTGCCGGACATCGGCGACTACAAGGACGTGCCCGTCA
TCGAGATCGCCGTGAAGATCGGCGACACGGTCGAGAAGGAGCAGTCGCTCGTCACGCTCGAATCGGACAAGGCGACGATG
GACGTGCCGAGCCCGGCCGCGGGCGTCGTCAAGGACATCAAGGTGAAGGTCGGCGATGCGGTGTCGGAAGGTTCGCTGAT
CGTCGTGCTCGAAGCGTCGGGCGGCGCCGCCGCGAGCGCGCCGCAGGCGGCCGCGCCCGCCCCCGCGCCGGCTCCGGCTC
CGGCGCCCGCGCCGCAGGCCGCACCCGCGGCTGCGCCGGCCCCCGCGCAGGCACCGGCACCCGCCGCGAGCGGCGAGTAC
CGCGCGAGCCACGCGTCGCCGTCGGTGCGCAAGTTCGCGCGCGAGCTCGGCGTCGACGTGTCGCGCGTCACGGGCACGGG
GCCGAAGAGCCGCATCACGAAGGACGACGTCACCGCGTTCGTGAAGGGCGTGATGACGGGACAGCGCGCGGCGCCCGGCG
CCGCGGCCGCGCCCGCGGGCGGCGGCGAGCTGAACCTGCTGCCGTGGCCGAAGGTCGACTTCTCGAAGTTCGGCCCGTTC
GAGGCGAAGCCGCTGTCGCGCATCAAGAAGATCTCGGGCGCGAACCTGCATCGCAACTGGGTGATGATCCCGCACGTCAC
GAACAACGACGAGGCGGACATCACCGAGCTCGAAGCGCTGCGCGTGCAACTGAACAAGGAGCACGAGAAGGCGGGCGTGA
AGTTCACGATGCTCGCGTTCGTGATCAAGGCGGTCGTCGCCGCGCTGAAGAAGTTCCCGACCTTCAACGCGAGCCTCGAC
GGCGACAACCTCGTGTTCAAGCAGTACTACCACATCGGTTTCGCCGCCGACACGCCGAACGGCCTCGTCGTGCCGGTGAT
TCGCGACGCGGACAAGAAGGGGCTCGTCGACATCGCGAAGGAAATGGCCGAGCTGTCGAAGGCCGCGCGCGAAGGCAAGC
TCAAGCCGGACCAGATGCAGGGCGGCTGCTTCTCGATCTCGTCGCTTGGCGGGATCGGCGGCACGCACTTCACGCCGATC
ATCAATGCGCCGGAAGTGGCGATCCTCGGGCTGTCGCGCGGCCAGATGAAGCCGGTGTGGGACGGCAAGCAGTTTGTGCC
GCGCCTCATGCTGCCGCTGTCGCTGTCGTATGACCATCGCGTGATCGATGGCGCGGAAGCCGCGCGGTTCAATGCGTATC
TCGGCGCGTTGCTTGCCGATTTCCGTCGCATCATTCTTTGA

Upstream 100 bases:

>100_bases
CGCTGTCGACGTTTTCGACGAACGAAGCGGAGGCGGGTGGCGCGGCTGCGCCTTGCATGGGATCGGAGCGGCCGCTCCGA
TCGACAGGAGACTGAAACAG

Downstream 100 bases:

>100_bases
TGAGCGTGGGAGTGGCTCGCGGGGGCGTGGGTTTTTTGTCGGTGATCCGCGCTTCGTCGTTGAACCTGTTGCGCTAACGG
TCTATCAGCGTCGCCCCTGC

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MSQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEVKVKVGDAVSQGSLIVLLDGA
QAAAQPAQANGAATSAAQPAAAPAAAPAPAAAAGGGTVDVKVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATM
DVPSPAAGVVKDIKVKVGDAVSEGSLIVVLEASGGAAASAPQAAAPAPAPAPAPAPAPQAAPAAAPAPAQAPAPAASGEY
RASHASPSVRKFARELGVDVSRVTGTGPKSRITKDDVTAFVKGVMTGQRAAPGAAAAPAGGGELNLLPWPKVDFSKFGPF
EAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASLD
GDNLVFKQYYHIGFAADTPNGLVVPVIRDADKKGLVDIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFTPI
INAPEVAILGLSRGQMKPVWDGKQFVPRLMLPLSLSYDHRVIDGAEAARFNAYLGALLADFRRIIL

Sequences:

>Translated_546_residues
MSQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEVKVKVGDAVSQGSLIVLLDGA
QAAAQPAQANGAATSAAQPAAAPAAAPAPAAAAGGGTVDVKVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATM
DVPSPAAGVVKDIKVKVGDAVSEGSLIVVLEASGGAAASAPQAAAPAPAPAPAPAPAPQAAPAAAPAPAQAPAPAASGEY
RASHASPSVRKFARELGVDVSRVTGTGPKSRITKDDVTAFVKGVMTGQRAAPGAAAAPAGGGELNLLPWPKVDFSKFGPF
EAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASLD
GDNLVFKQYYHIGFAADTPNGLVVPVIRDADKKGLVDIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFTPI
INAPEVAILGLSRGQMKPVWDGKQFVPRLMLPLSLSYDHRVIDGAEAARFNAYLGALLADFRRIIL
>Mature_545_residues
SQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEVKVKVGDAVSQGSLIVLLDGAQ
AAAQPAQANGAATSAAQPAAAPAAAPAPAAAAGGGTVDVKVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATMD
VPSPAAGVVKDIKVKVGDAVSEGSLIVVLEASGGAAASAPQAAAPAPAPAPAPAPAPQAAPAAAPAPAQAPAPAASGEYR
ASHASPSVRKFARELGVDVSRVTGTGPKSRITKDDVTAFVKGVMTGQRAAPGAAAAPAGGGELNLLPWPKVDFSKFGPFE
AKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASLDG
DNLVFKQYYHIGFAADTPNGLVVPVIRDADKKGLVDIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFTPII
NAPEVAILGLSRGQMKPVWDGKQFVPRLMLPLSLSYDHRVIDGAEAARFNAYLGALLADFRRIIL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=459, Percent_Identity=29.4117647058824, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI31711992, Length=423, Percent_Identity=32.6241134751773, Blast_Score=159, Evalue=5e-39,
Organism=Homo sapiens, GI203098816, Length=482, Percent_Identity=27.8008298755187, Blast_Score=141, Evalue=2e-33,
Organism=Homo sapiens, GI203098753, Length=438, Percent_Identity=28.5388127853881, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=34.8017621145374, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI260898739, Length=153, Percent_Identity=37.2549019607843, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1786305, Length=545, Percent_Identity=53.394495412844, Blast_Score=512, Evalue=1e-146,
Organism=Escherichia coli, GI1786946, Length=432, Percent_Identity=31.0185185185185, Blast_Score=189, Evalue=5e-49,
Organism=Caenorhabditis elegans, GI17537937, Length=424, Percent_Identity=28.5377358490566, Blast_Score=172, Evalue=5e-43,
Organism=Caenorhabditis elegans, GI17560088, Length=441, Percent_Identity=31.2925170068027, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=423, Percent_Identity=30.9692671394799, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17538894, Length=309, Percent_Identity=33.3333333333333, Blast_Score=121, Evalue=8e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=419, Percent_Identity=30.0715990453461, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6324258, Length=435, Percent_Identity=28.735632183908, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI18859875, Length=431, Percent_Identity=33.6426914153132, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=31.8965517241379, Blast_Score=127, Evalue=3e-29,
Organism=Drosophila melanogaster, GI20129315, Length=232, Percent_Identity=31.8965517241379, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI24645909, Length=207, Percent_Identity=35.7487922705314, Blast_Score=123, Evalue=4e-28,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 56296; Mature: 56165

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEV
CCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCCCEEE
KVKVGDAVSQGSLIVLLDGAQAAAQPAQANGAATSAAQPAAAPAAAPAPAAAAGGGTVDV
EEEECCCCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
KVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATMDVPSPAAGVVKDIKVKVGDA
ECCCCCCCCCCCEEEEEEECCCCHHCCCCEEEEECCCCEECCCCCHHHHHHHEEEEECCC
VSEGSLIVVLEASGGAAASAPQAAAPAPAPAPAPAPAPQAAPAAAPAPAQAPAPAASGEY
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RASHASPSVRKFARELGVDVSRVTGTGPKSRITKDDVTAFVKGVMTGQRAAPGAAAAPAG
CCCCCCHHHHHHHHHHCCEEEEECCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
GGELNLLPWPKVDFSKFGPFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEAL
CCEEEECCCCCCCHHHCCCCCCCHHHHHHHHCCCCEECCEEEEEEECCCCCCCHHHHHHH
RVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASLDGDNLVFKQYYHIGFAADTPN
HHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCC
GLVVPVIRDADKKGLVDIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFTPI
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCCCCCCCCCEE
INAPEVAILGLSRGQMKPVWDGKQFVPRLMLPLSLSYDHRVIDGAEAARFNAYLGALLAD
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCEECCCCCEEECCCHHHHHHHHHHHHHHH
FRRIIL
HHHHHC
>Mature Secondary Structure 
SQAIEVKVPDIGDYKDVPVIEVLVKPGDAVEPEQSLVTLESDKATMDVPSPSAGTVKEV
CCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCCCEEECCCCCCCCCEEE
KVKVGDAVSQGSLIVLLDGAQAAAQPAQANGAATSAAQPAAAPAAAPAPAAAAGGGTVDV
EEEECCCCCCCCEEEEECCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEE
KVPDIGDYKDVPVIEIAVKIGDTVEKEQSLVTLESDKATMDVPSPAAGVVKDIKVKVGDA
ECCCCCCCCCCCEEEEEEECCCCHHCCCCEEEEECCCCEECCCCCHHHHHHHEEEEECCC
VSEGSLIVVLEASGGAAASAPQAAAPAPAPAPAPAPAPQAAPAAAPAPAQAPAPAASGEY
CCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RASHASPSVRKFARELGVDVSRVTGTGPKSRITKDDVTAFVKGVMTGQRAAPGAAAAPAG
CCCCCCHHHHHHHHHHCCEEEEECCCCCHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCC
GGELNLLPWPKVDFSKFGPFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEAL
CCEEEECCCCCCCHHHCCCCCCCHHHHHHHHCCCCEECCEEEEEEECCCCCCCHHHHHHH
RVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASLDGDNLVFKQYYHIGFAADTPN
HHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEECCCC
GLVVPVIRDADKKGLVDIAKEMAELSKAAREGKLKPDQMQGGCFSISSLGGIGGTHFTPI
CEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHCCCEEEECCCCCCCCCCCCEE
INAPEVAILGLSRGQMKPVWDGKQFVPRLMLPLSLSYDHRVIDGAEAARFNAYLGALLAD
CCCCCEEEEECCCCCCCCCCCCHHHHHHHHCCEECCCCCEEECCCHHHHHHHHHHHHHHH
FRRIIL
HHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]