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The map label for this gene is yjfH [C]

Identifier: 53719753

GI number: 53719753

Start: 2574492

End: 2575280

Strand: Reverse

Name: yjfH [C]

Synonym: BPSL2144

Alternate gene names: 53719753

Gene position: 2575280-2574492 (Counterclockwise)

Preceding gene: 53719754

Following gene: 53719751

Centisome position: 63.2

GC content: 71.23

Gene sequence:

>789_bases
ATGAAAGCGATCACTTCCCGCGACAATCCGCTGTACAAGCGCCTGAAGGCGCTCGCGGGATCGACGCACCAGCAGCGGCG
CGGCGAGCAGGCGCTCCTCGAAGGGTTCCATCTCGCGAGCGCCTATCTCGATGCGGCCGGACAGCCGCAGCTGTGCGTCG
TCACCGACGGCGCGCTCGCGCATGAGGAGGCGCGCGGGATCGTCGCGCGCATCGAGCCGGCGCGGGTCGTCACGTTGCCC
GATGTGCTGTTCGGACAGCTATCGAACGTCGTCAACGGCGTCGGGCTGCTGCTGCTCGTCGCCCGGCCGGCGCCGGCGCT
GCCGGCGCGTGTCGCGGGCACGTCGGTCGTGCTCGACGGCGTGCAGGACGCGGGCAACGTCGGCTCGATCCTGCGCAGCG
CGGCCGCGGCGGGCGTGGAGCGGGTGTTCTGCGCGCCGGGCACCGCCTACGCGTGGTCGTCGAAGGTGCTGCGCTCGGCG
ATGGGCGCGCATTTCCTGCTCGACATTCACGAGGACGTCGACGCCGGCACGCTGATCGAGCGGCTCGCGATTCCGGTCGC
GCTGACCGATTCGCACGGCGCGCGCGCGATCTACGACTGCGATCTGGCCGGGCCGCTCGCGTGGGTGTTCGGCAACGAAG
GAGCGGGCGTGTCCGCCCGGTGGCGCGAGGCGGCGACGCATCGCGTGACGATTCCGCAGCCGGGCGGAATGGAATCGCTC
AATGTGGCCGCGGCAGCGGCGGTTTGTCTCTTCGAGCAATGCCGGCAGCAACGCGCGCTTCGCGCGTGA

Upstream 100 bases:

>100_bases
ACGGTACGCCGCAGCATCTGGCCGCCTTGCGCGAGCATGGGCCGTGCGAGCATCACCGCCGCTCGTTCGCGCCGGTGCGC
GAAGCCTTCGATTTGATTCG

Downstream 100 bases:

>100_bases
CGCACGCGCTTTCTCGCTCGCTCTCGCTCTTCTTCGCAATCGGCTTGCCTCGCTGACGCGGCGACGCCGCGACAGCGCCG
GCGAAGGCCGTCGCCGCGAC

Product: putative RNA methylase protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MKAITSRDNPLYKRLKALAGSTHQQRRGEQALLEGFHLASAYLDAAGQPQLCVVTDGALAHEEARGIVARIEPARVVTLP
DVLFGQLSNVVNGVGLLLLVARPAPALPARVAGTSVVLDGVQDAGNVGSILRSAAAAGVERVFCAPGTAYAWSSKVLRSA
MGAHFLLDIHEDVDAGTLIERLAIPVALTDSHGARAIYDCDLAGPLAWVFGNEGAGVSARWREAATHRVTIPQPGGMESL
NVAAAAAVCLFEQCRQQRALRA

Sequences:

>Translated_262_residues
MKAITSRDNPLYKRLKALAGSTHQQRRGEQALLEGFHLASAYLDAAGQPQLCVVTDGALAHEEARGIVARIEPARVVTLP
DVLFGQLSNVVNGVGLLLLVARPAPALPARVAGTSVVLDGVQDAGNVGSILRSAAAAGVERVFCAPGTAYAWSSKVLRSA
MGAHFLLDIHEDVDAGTLIERLAIPVALTDSHGARAIYDCDLAGPLAWVFGNEGAGVSARWREAATHRVTIPQPGGMESL
NVAAAAAVCLFEQCRQQRALRA
>Mature_262_residues
MKAITSRDNPLYKRLKALAGSTHQQRRGEQALLEGFHLASAYLDAAGQPQLCVVTDGALAHEEARGIVARIEPARVVTLP
DVLFGQLSNVVNGVGLLLLVARPAPALPARVAGTSVVLDGVQDAGNVGSILRSAAAAGVERVFCAPGTAYAWSSKVLRSA
MGAHFLLDIHEDVDAGTLIERLAIPVALTDSHGARAIYDCDLAGPLAWVFGNEGAGVSARWREAATHRVTIPQPGGMESL
NVAAAAAVCLFEQCRQQRALRA

Specific function: Unknown

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790623, Length=209, Percent_Identity=29.6650717703349, Blast_Score=75, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24666840, Length=284, Percent_Identity=26.056338028169, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27482; Mature: 27482

Theoretical pI: Translated: 7.54; Mature: 7.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAITSRDNPLYKRLKALAGSTHQQRRGEQALLEGFHLASAYLDAAGQPQLCVVTDGALA
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHH
HEEARGIVARIEPARVVTLPDVLFGQLSNVVNGVGLLLLVARPAPALPARVAGTSVVLDG
HHHHCCEEEEECCCEEEECHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHCCCHHEEEC
VQDAGNVGSILRSAAAAGVERVFCAPGTAYAWSSKVLRSAMGAHFLLDIHEDVDAGTLIE
CCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHH
RLAIPVALTDSHGARAIYDCDLAGPLAWVFGNEGAGVSARWREAATHRVTIPQPGGMESL
HHHCCEEEECCCCCCEEEECCCCCCEEEEECCCCCCCCHHHHHHCCCEEECCCCCCCCHH
NVAAAAAVCLFEQCRQQRALRA
HHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKAITSRDNPLYKRLKALAGSTHQQRRGEQALLEGFHLASAYLDAAGQPQLCVVTDGALA
CCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCHHH
HEEARGIVARIEPARVVTLPDVLFGQLSNVVNGVGLLLLVARPAPALPARVAGTSVVLDG
HHHHCCEEEEECCCEEEECHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHCCCHHEEEC
VQDAGNVGSILRSAAAAGVERVFCAPGTAYAWSSKVLRSAMGAHFLLDIHEDVDAGTLIE
CCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHHHCCCEEEEEECCCCCHHHHHH
RLAIPVALTDSHGARAIYDCDLAGPLAWVFGNEGAGVSARWREAATHRVTIPQPGGMESL
HHHCCEEEECCCCCCEEEECCCCCCEEEEECCCCCCCCHHHHHHCCCEEECCCCCCCCHH
NVAAAAAVCLFEQCRQQRALRA
HHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]