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The map label for this gene is ynfL [H]

Identifier: 53719643

GI number: 53719643

Start: 2427413

End: 2428306

Strand: Reverse

Name: ynfL [H]

Synonym: BPSL2032

Alternate gene names: 53719643

Gene position: 2428306-2427413 (Counterclockwise)

Preceding gene: 53719647

Following gene: 53719642

Centisome position: 59.6

GC content: 67.45

Gene sequence:

>894_bases
ATGGAATCCAAGCATCTACGTTATTTTCTGGCCATCGCCGATACCGGCAGCCTCACTCATGCGGCGATTCGTCTCGGTGT
CGCGCAGCCCGCGCTCAGCCACGCGCTGACGAAGATGGAGCGAGAGCTCGGCGTGAAGCTGTTCGACCGCTCGAGGCGCG
GCGCGACGCTCACGACGGCGGGCGAGGCGATCGTCGACGACATTCGCCTGAGCCTCGCGCGAATGGACGCCGCCGCGCGC
CGCGCGAGCGAGATCGGCGCGCAGCGGGCCGGGCGGCTGAGCATCGGCTTCGTATCGGCCGCGCTGTTCGATACGTTGCC
CTCCGCCGTGTGCGCGCTGCGCGCGGCATCGCCGCGCGTCGATCTCGTGATGCGCGAGATGAGCAACGCGGAGCAGGTGG
TCGCGCTCGAGCGCGGCGAGATCGATATCGGGATTCTGCATACGCCGGTGGCGATCGGCGGCCCGGTTCACGAGAAGCTG
ATTCGGCGCGATCCGCTGATCGCCGTGATTCCGAAGTCGTTTCCTCGCCAGCCGGACGGCTCGATCACGCTGAGGGAACT
GTCGCGCGCGGGGCTCATCTGGTTTCCGCACGAGCAGCTGCCGTCGATCCGCGCGGCGATCCTCAGCGCGTTTCGGCTGG
CGGGCCATCCGATCGAGGTCGTCCAGGATGCGAATCGCACGCTGACCGTGATCGCCTGCGTCGCGGCGGGTTGCGGCGTA
TCGCTGCTGCCGTCGTCGATCCGCACGCTGACGTTCGAAGGCGTCGATTTCAGTCCGGTCTCCGACGGGCATGCGTTGCC
GGAATTTCCGTTGACGGCGATCTGGCCGAAGCGGTCGCGCCGAACGCTCGCCGATCGGTTCGCGGAGTTGTTGCCCCGGT
TTCAGCCGAACTGA

Upstream 100 bases:

>100_bases
GGGGCGAGCGCGGAATGAAAGCGTGTATTCGCCAATCAGCCCGCCGATTCGGCGGCGCGGCGGCGAGCGTCGAAAAGCAG
TCTCATTGCGCCGAGTGATG

Downstream 100 bases:

>100_bases
GCGCACGGGCGGGACGAGCGGCGGGCGGCGCACGCGCGCGTGCGCGCGTTCCGGCCGGTGCCGCATGCGCCGCTGCCGCC
ATGCCGCTCGTCCGGATGCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MESKHLRYFLAIADTGSLTHAAIRLGVAQPALSHALTKMERELGVKLFDRSRRGATLTTAGEAIVDDIRLSLARMDAAAR
RASEIGAQRAGRLSIGFVSAALFDTLPSAVCALRAASPRVDLVMREMSNAEQVVALERGEIDIGILHTPVAIGGPVHEKL
IRRDPLIAVIPKSFPRQPDGSITLRELSRAGLIWFPHEQLPSIRAAILSAFRLAGHPIEVVQDANRTLTVIACVAAGCGV
SLLPSSIRTLTFEGVDFSPVSDGHALPEFPLTAIWPKRSRRTLADRFAELLPRFQPN

Sequences:

>Translated_297_residues
MESKHLRYFLAIADTGSLTHAAIRLGVAQPALSHALTKMERELGVKLFDRSRRGATLTTAGEAIVDDIRLSLARMDAAAR
RASEIGAQRAGRLSIGFVSAALFDTLPSAVCALRAASPRVDLVMREMSNAEQVVALERGEIDIGILHTPVAIGGPVHEKL
IRRDPLIAVIPKSFPRQPDGSITLRELSRAGLIWFPHEQLPSIRAAILSAFRLAGHPIEVVQDANRTLTVIACVAAGCGV
SLLPSSIRTLTFEGVDFSPVSDGHALPEFPLTAIWPKRSRRTLADRFAELLPRFQPN
>Mature_297_residues
MESKHLRYFLAIADTGSLTHAAIRLGVAQPALSHALTKMERELGVKLFDRSRRGATLTTAGEAIVDDIRLSLARMDAAAR
RASEIGAQRAGRLSIGFVSAALFDTLPSAVCALRAASPRVDLVMREMSNAEQVVALERGEIDIGILHTPVAIGGPVHEKL
IRRDPLIAVIPKSFPRQPDGSITLRELSRAGLIWFPHEQLPSIRAAILSAFRLAGHPIEVVQDANRTLTVIACVAAGCGV
SLLPSSIRTLTFEGVDFSPVSDGHALPEFPLTAIWPKRSRRTLADRFAELLPRFQPN

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=287, Percent_Identity=33.1010452961672, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1788887, Length=278, Percent_Identity=29.4964028776978, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI2367136, Length=282, Percent_Identity=29.0780141843972, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1788297, Length=250, Percent_Identity=32.4, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI157672245, Length=251, Percent_Identity=31.8725099601594, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1788748, Length=242, Percent_Identity=23.5537190082645, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI87081904, Length=171, Percent_Identity=31.5789473684211, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790399, Length=249, Percent_Identity=26.5060240963855, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI145693105, Length=249, Percent_Identity=24.0963855421687, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789506, Length=281, Percent_Identity=23.4875444839858, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1787601, Length=141, Percent_Identity=29.7872340425532, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1790262, Length=250, Percent_Identity=24.4, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1787806, Length=279, Percent_Identity=23.2974910394265, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790208, Length=264, Percent_Identity=24.2424242424242, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1787530, Length=256, Percent_Identity=26.171875, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32179; Mature: 32179

Theoretical pI: Translated: 10.25; Mature: 10.25

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESKHLRYFLAIADTGSLTHAAIRLGVAQPALSHALTKMERELGVKLFDRSRRGATLTTA
CCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHCCCCCCCCEEEEC
GEAIVDDIRLSLARMDAAARRASEIGAQRAGRLSIGFVSAALFDTLPSAVCALRAASPRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCCCHH
DLVMREMSNAEQVVALERGEIDIGILHTPVAIGGPVHEKLIRRDPLIAVIPKSFPRQPDG
HHHHHHHCCCHHEEEEECCCEEEEEEECCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCC
SITLRELSRAGLIWFPHEQLPSIRAAILSAFRLAGHPIEVVQDANRTLTVIACVAAGCGV
CEEHHHHHCCCEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCCH
SLLPSSIRTLTFEGVDFSPVSDGHALPEFPLTAIWPKRSRRTLADRFAELLPRFQPN
HHCCCCHHEEEECCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MESKHLRYFLAIADTGSLTHAAIRLGVAQPALSHALTKMERELGVKLFDRSRRGATLTTA
CCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHCCCCCCCCEEEEC
GEAIVDDIRLSLARMDAAARRASEIGAQRAGRLSIGFVSAALFDTLPSAVCALRAASPRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCCCCHH
DLVMREMSNAEQVVALERGEIDIGILHTPVAIGGPVHEKLIRRDPLIAVIPKSFPRQPDG
HHHHHHHCCCHHEEEEECCCEEEEEEECCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCC
SITLRELSRAGLIWFPHEQLPSIRAAILSAFRLAGHPIEVVQDANRTLTVIACVAAGCGV
CEEHHHHHCCCEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHCCCCHHHHHHHHHHCCCH
SLLPSSIRTLTFEGVDFSPVSDGHALPEFPLTAIWPKRSRRTLADRFAELLPRFQPN
HHCCCCHHEEEECCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]