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The map label for this gene is cht60 [H]

Identifier: 53719627

GI number: 53719627

Start: 2404072

End: 2406120

Strand: Reverse

Name: cht60 [H]

Synonym: BPSL2015

Alternate gene names: 53719627

Gene position: 2406120-2404072 (Counterclockwise)

Preceding gene: 53719629

Following gene: 53719626

Centisome position: 59.05

GC content: 70.18

Gene sequence:

>2049_bases
ATGCGCAAGAAAAGCTTGGTTCATTTGCCCGTCGCGCTGGCGGTGGCCGCGGCGCTCGGCGCGTGCTCGGGCGACGACGA
CGCGACGCTCGAGTCGCGCGCGGACGCGATCGTCGAACGCATGACGACCCGGCAGAAGGTCGGCCAAAAACTGATGATGG
CGTTCCGGTACTGGTGCCCCGACGGGCAGCCCGCGTGCACGACCGGCATGACCGAATTTCCGGACGCCGCGCGCGACGCG
CTGCGCGAGAACGGCATTGGCGGCGTGATCCTGTTCTCGAACAACCTGACGGGCATCGAGCAGACGCGCCGGCTGATCGA
CGGCATTCGGGCCGCGCCCGCGGCCGACAGCCCGCTCGGGCTGATGATCGGCATCGACGAGGAGGGCGGCAACGTGTTTC
GCCTGCCGCGCGTCGAGGCGACCGCGTTCGCCGGCAACATGGCGCTCGGCGCGGCGTACGAAGCGACGCGGGACGACCGG
CTCGCGTACGACATGGGCCGCGTGCTCGCCGCGGAGATCGCGGCGGTCGGCTTCAACGTGAATTTCGCGCCGGACGTCGA
CGTCAACAGCAATCCGCTCAATCCGGTGATCAACGTGCGCGCGTTCGGCGACGATCCGGCGACGATCGGCCTGCTCGGCC
GGCGCATGGTGCAGGGCATGAAGAGCGAGCGCGTGATCGGCACGTTCAAGCATTTTCCCGGGCACGGCGACACGGATACC
GATTCGCACTACGGACTGCCCGTCGTGATCAAGTCGCGCGCCGACGCCTACGCGATCGATCTCGCGCCGTACCGGCAGGC
GATCGAAGCGGGCGAGGCGCCCGACATGATCATGACCGCGCACATCCAGTATCCGTCGCTCGACGACACCCGCGTCGCGA
CCCGCACGGGCGAGCAGATGATCGCGCCCGCGACGATGTCGCGGCGCATCCAGCACGATATCCTGCGCGGCGAGTTCGGC
TATCAGGGCGTGACGATCACCGACGCGCTCGACATGAAGGGCATCGCCGGCTTCTTCGACGAGGACGACGCGGTCGTCAA
GGTGTTCCAGGCGGACGTCGACATTGCGCTGATGCCCGTCGAGTTTCGCACCGCGGCCGATGCCGGGCGCCTGGCGGCGC
TCGTCGATCGCGTCGCCGCGGCGGTCGATTCGGGGCGGATCGATCGCGCCGAGTTCGATCGCTCGGTGCGCCGTATCGTG
CTGACGAAGCTGCGCCACGGCATCGTCGCGTCGGATCGCGGCCGGCCGGTCGACGAGCTGGCATCGATCGGCGGGCCCGC
GCACCGCGCGATCGAGCGCGACATCGCGCAAAAATCGATCACCGTGCTGCGCAACGAGAACGGCGCGCTGCCGCTGCAGG
CCGCGGGCCGGCGCATCTTCATCCTGACGCCGTGGGGCGAGCAGGCCGAGGCGATGCGGCGGCGTTTCGTCGAGCTTGGC
CATCCGCTCGTCACGGGCGCGAAGCTGAGCACGATCACGTGGGCCGAACAGCAGCAGGCGATCGATGCGGCCGACGTCGT
GATCGTCGGCACGCTGTCGACGGGCGTCACGCCCGTCGAGCACAACGGGGATCCGAACGCGCGCGTGAGCCCGCCGGCGC
CGTCGGCCGTGCGGATGCGGCAGGCCGCGCCCGCGAACGGCGAGGAGGAGGGTTCGGTGATCTTCGACCACGTCGAGCGG
GCGGACGCGGCGAAGGACATCGGCGCGCGCCCGAGCGTGCTCGCGGCGATCGCGGCGCCGAGCGAGGCGCAGCAGATGCG
CGACGCGATGGACTACGCGAAGGCGCGGCGCAAGACCGTGATCCACGTGACGATGCGCGCGCCGTACGACGTGATCAGCT
ACGACGATGTCGCGGACGCGACGCTCGCCACCTACGCGTACTACGGCTACGAAGGCGGCTTGCGCGGCCCGTCGCTGCCC
GCGGCCGTCGACGCGATGCTCGGCGTCGCGCGGCCGGTCGGCAGGCTGCCGGTCGCGATCCACGCACTGAACGCGGACGG
ATCGACGGGGCCGCTGCGCTACGCGCGCGGATTCGGGTTGCAGTACTGA

Upstream 100 bases:

>100_bases
GTTCCGGAGTACCGATGAACCGAAAGCCGATAGGGATGCCGGATGACGAATGAAACGACGTGAATGCCGCGGGAATGCGC
GCGGCAAGGATTCGGTGAAG

Downstream 100 bases:

>100_bases
GGCCGGACGCCGGCGACGCCGCGTCGCTGTCGGCGCGGCGTCGAGCGACACGCCGCGCGCGCAGGCGGGCTGCGGCGGCG
CGGCCGATCGCCGCGCGTTA

Product: putative membrane attached glycosyl hydrolase

Products: NA

Alternate protein names: Beta-N-acetylhexosaminidase; Chitobiase; N-acetyl-beta-glucosaminidase [H]

Number of amino acids: Translated: 682; Mature: 682

Protein sequence:

>682_residues
MRKKSLVHLPVALAVAAALGACSGDDDATLESRADAIVERMTTRQKVGQKLMMAFRYWCPDGQPACTTGMTEFPDAARDA
LRENGIGGVILFSNNLTGIEQTRRLIDGIRAAPAADSPLGLMIGIDEEGGNVFRLPRVEATAFAGNMALGAAYEATRDDR
LAYDMGRVLAAEIAAVGFNVNFAPDVDVNSNPLNPVINVRAFGDDPATIGLLGRRMVQGMKSERVIGTFKHFPGHGDTDT
DSHYGLPVVIKSRADAYAIDLAPYRQAIEAGEAPDMIMTAHIQYPSLDDTRVATRTGEQMIAPATMSRRIQHDILRGEFG
YQGVTITDALDMKGIAGFFDEDDAVVKVFQADVDIALMPVEFRTAADAGRLAALVDRVAAAVDSGRIDRAEFDRSVRRIV
LTKLRHGIVASDRGRPVDELASIGGPAHRAIERDIAQKSITVLRNENGALPLQAAGRRIFILTPWGEQAEAMRRRFVELG
HPLVTGAKLSTITWAEQQQAIDAADVVIVGTLSTGVTPVEHNGDPNARVSPPAPSAVRMRQAAPANGEEEGSVIFDHVER
ADAAKDIGARPSVLAAIAAPSEAQQMRDAMDYAKARRKTVIHVTMRAPYDVISYDDVADATLATYAYYGYEGGLRGPSLP
AAVDAMLGVARPVGRLPVAIHALNADGSTGPLRYARGFGLQY

Sequences:

>Translated_682_residues
MRKKSLVHLPVALAVAAALGACSGDDDATLESRADAIVERMTTRQKVGQKLMMAFRYWCPDGQPACTTGMTEFPDAARDA
LRENGIGGVILFSNNLTGIEQTRRLIDGIRAAPAADSPLGLMIGIDEEGGNVFRLPRVEATAFAGNMALGAAYEATRDDR
LAYDMGRVLAAEIAAVGFNVNFAPDVDVNSNPLNPVINVRAFGDDPATIGLLGRRMVQGMKSERVIGTFKHFPGHGDTDT
DSHYGLPVVIKSRADAYAIDLAPYRQAIEAGEAPDMIMTAHIQYPSLDDTRVATRTGEQMIAPATMSRRIQHDILRGEFG
YQGVTITDALDMKGIAGFFDEDDAVVKVFQADVDIALMPVEFRTAADAGRLAALVDRVAAAVDSGRIDRAEFDRSVRRIV
LTKLRHGIVASDRGRPVDELASIGGPAHRAIERDIAQKSITVLRNENGALPLQAAGRRIFILTPWGEQAEAMRRRFVELG
HPLVTGAKLSTITWAEQQQAIDAADVVIVGTLSTGVTPVEHNGDPNARVSPPAPSAVRMRQAAPANGEEEGSVIFDHVER
ADAAKDIGARPSVLAAIAAPSEAQQMRDAMDYAKARRKTVIHVTMRAPYDVISYDDVADATLATYAYYGYEGGLRGPSLP
AAVDAMLGVARPVGRLPVAIHALNADGSTGPLRYARGFGLQY
>Mature_682_residues
MRKKSLVHLPVALAVAAALGACSGDDDATLESRADAIVERMTTRQKVGQKLMMAFRYWCPDGQPACTTGMTEFPDAARDA
LRENGIGGVILFSNNLTGIEQTRRLIDGIRAAPAADSPLGLMIGIDEEGGNVFRLPRVEATAFAGNMALGAAYEATRDDR
LAYDMGRVLAAEIAAVGFNVNFAPDVDVNSNPLNPVINVRAFGDDPATIGLLGRRMVQGMKSERVIGTFKHFPGHGDTDT
DSHYGLPVVIKSRADAYAIDLAPYRQAIEAGEAPDMIMTAHIQYPSLDDTRVATRTGEQMIAPATMSRRIQHDILRGEFG
YQGVTITDALDMKGIAGFFDEDDAVVKVFQADVDIALMPVEFRTAADAGRLAALVDRVAAAVDSGRIDRAEFDRSVRRIV
LTKLRHGIVASDRGRPVDELASIGGPAHRAIERDIAQKSITVLRNENGALPLQAAGRRIFILTPWGEQAEAMRRRFVELG
HPLVTGAKLSTITWAEQQQAIDAADVVIVGTLSTGVTPVEHNGDPNARVSPPAPSAVRMRQAAPANGEEEGSVIFDHVER
ADAAKDIGARPSVLAAIAAPSEAQQMRDAMDYAKARRKTVIHVTMRAPYDVISYDDVADATLATYAYYGYEGGLRGPSLP
AAVDAMLGVARPVGRLPVAIHALNADGSTGPLRYARGFGLQY

Specific function: Most active towards p-nitrophenyl-N-acetyl-beta-D- glucosaminide(PNP-beta-GlcNAc) and diacetylchitobiose [H]

COG id: COG1472

COG function: function code G; Beta-glucosidase-related glycosidases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787350, Length=259, Percent_Identity=31.2741312741313, Blast_Score=108, Evalue=8e-25,
Organism=Escherichia coli, GI1788453, Length=689, Percent_Identity=23.0769230769231, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019800
- InterPro:   IPR001764
- InterPro:   IPR017853 [H]

Pfam domain/function: PF00933 Glyco_hydro_3 [H]

EC number: =3.2.1.52 [H]

Molecular weight: Translated: 73127; Mature: 73127

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00775 GLYCOSYL_HYDROL_F3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKKSLVHLPVALAVAAALGACSGDDDATLESRADAIVERMTTRQKVGQKLMMAFRYWCP
CCCCCEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DGQPACTTGMTEFPDAARDALRENGIGGVILFSNNLTGIEQTRRLIDGIRAAPAADSPLG
CCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCE
LMIGIDEEGGNVFRLPRVEATAFAGNMALGAAYEATRDDRLAYDMGRVLAAEIAAVGFNV
EEEEEECCCCCEEEECCCCHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
NFAPDVDVNSNPLNPVINVRAFGDDPATIGLLGRRMVQGMKSERVIGTFKHFPGHGDTDT
CCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEHHHCCCCCCCCCC
DSHYGLPVVIKSRADAYAIDLAPYRQAIEAGEAPDMIMTAHIQYPSLDDTRVATRTGEQM
CCCCCCEEEEECCCCEEEEECHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHCCCHH
IAPATMSRRIQHDILRGEFGYQGVTITDALDMKGIAGFFDEDDAVVKVFQADVDIALMPV
CCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEE
EFRTAADAGRLAALVDRVAAAVDSGRIDRAEFDRSVRRIVLTKLRHGIVASDRGRPVDEL
EECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHH
ASIGGPAHRAIERDIAQKSITVLRNENGALPLQAAGRRIFILTPWGEQAEAMRRRFVELG
HHCCCHHHHHHHHHHHHHHEEEEECCCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHC
HPLVTGAKLSTITWAEQQQAIDAADVVIVGTLSTGVTPVEHNGDPNARVSPPAPSAVRMR
CCEECCCEEEEEEEHHHHHCCCCCCEEEEEECCCCCCEECCCCCCCCEECCCCCHHHHHH
QAAPANGEEEGSVIFDHVERADAAKDIGARPSVLAAIAAPSEAQQMRDAMDYAKARRKTV
HCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEE
IHVTMRAPYDVISYDDVADATLATYAYYGYEGGLRGPSLPAAVDAMLGVARPVGRLPVAI
EEEEEECCCCCCCCCCHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEE
HALNADGSTGPLRYARGFGLQY
EEECCCCCCCCHHHHCCCCCCC
>Mature Secondary Structure
MRKKSLVHLPVALAVAAALGACSGDDDATLESRADAIVERMTTRQKVGQKLMMAFRYWCP
CCCCCEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DGQPACTTGMTEFPDAARDALRENGIGGVILFSNNLTGIEQTRRLIDGIRAAPAADSPLG
CCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCCE
LMIGIDEEGGNVFRLPRVEATAFAGNMALGAAYEATRDDRLAYDMGRVLAAEIAAVGFNV
EEEEEECCCCCEEEECCCCHHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHCCCC
NFAPDVDVNSNPLNPVINVRAFGDDPATIGLLGRRMVQGMKSERVIGTFKHFPGHGDTDT
CCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCCEEEHHHCCCCCCCCCC
DSHYGLPVVIKSRADAYAIDLAPYRQAIEAGEAPDMIMTAHIQYPSLDDTRVATRTGEQM
CCCCCCEEEEECCCCEEEEECHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHHHCCCHH
IAPATMSRRIQHDILRGEFGYQGVTITDALDMKGIAGFFDEDDAVVKVFQADVDIALMPV
CCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEE
EFRTAADAGRLAALVDRVAAAVDSGRIDRAEFDRSVRRIVLTKLRHGIVASDRGRPVDEL
EECCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCEECCCCCCHHHH
ASIGGPAHRAIERDIAQKSITVLRNENGALPLQAAGRRIFILTPWGEQAEAMRRRFVELG
HHCCCHHHHHHHHHHHHHHEEEEECCCCCCCHHHCCCEEEEECCCCHHHHHHHHHHHHHC
HPLVTGAKLSTITWAEQQQAIDAADVVIVGTLSTGVTPVEHNGDPNARVSPPAPSAVRMR
CCEECCCEEEEEEEHHHHHCCCCCCEEEEEECCCCCCEECCCCCCCCEECCCCCHHHHHH
QAAPANGEEEGSVIFDHVERADAAKDIGARPSVLAAIAAPSEAQQMRDAMDYAKARRKTV
HCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHCCCEE
IHVTMRAPYDVISYDDVADATLATYAYYGYEGGLRGPSLPAAVDAMLGVARPVGRLPVAI
EEEEEECCCCCCCCCCHHHHHHHHHEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEE
HALNADGSTGPLRYARGFGLQY
EEECCCCCCCCHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8063094 [H]