Click here to switch to the map view.

The map label for this gene is oprM [H]

Identifier: 53719415

GI number: 53719415

Start: 2146271

End: 2147821

Strand: Reverse

Name: oprM [H]

Synonym: BPSL1802

Alternate gene names: 53719415

Gene position: 2147821-2146271 (Counterclockwise)

Preceding gene: 53719416

Following gene: 53719414

Centisome position: 52.71

GC content: 74.6

Gene sequence:

>1551_bases
GTGAAAGCGATGATGAAACCCCGGGCCCGGCGGCGCGGCGCGCGCGCAGCGCGGCGGCCGAACGGGCCGCGGGCCTGGCC
GCTCGCGGTCGCGGCCGCGCTCGTCGCGGGCTGCACGCTCGCGCCGCGCTACGAGCGGCCGGCCGCGCCCGTGCCGGCGC
ATTATTGGTCGACGGCGACGGGCGCGCCGCGCGAGGCGGGCCCGGCGGCCGCCGGCGGCCCGCGCGCGATGCCGGACGCG
CGCCGCGGCGACGCCGGGCGCGACGCCCGCGACGCGCGGCTCGACGACTGGCGCGCGTATTTCACCGATCCGGCGCTGCG
CGCGCTGATCGACGCGGCGCTCGCGAACAATCGCGACCTGCGGATCGCGACGCTGCGTATCCAGGAAGCGCGCGGCCTCT
ACGGCGTCGCGCGCGCGGACCGGCTGCCGTCGATCGACGGCAGCCTCGGCTACGAGCGCACGCGGCTTTACGACCCGGTG
CTGCGCGAGAGCGCGACGAGCTCGCTGTATCGCGCGAGTGTCGGCGTGAGCGCGTTCGAGATCGATCTGTTCGGCCGCGT
GAAGAGCCTCTCGGACGCGGCGCTCGCCGAGTACTTCGCGACCGCCGAGGCGCAGCGCGCGGCGCGCATCAGCCTGATCG
CCGAAGTCGCGTCCGCGTACGTGACCGAGCGCGCGCTCGTCGATCAACTGGGGCTCGCCGAACGCACGCTCGCCGCGCGC
GACGCGGCCTACGCGCTCACGCAGCGCCGTTACGCGGCCGGCACGAGCACGGCGATCGAGCTGCGCACGGCCGAGATGCT
CGTCGCGTCGGCGCGCGCGTCGAAGGCGGCGCTCGAGCGCGAGCACACGCAGGCGGCGAGCGCGCTGAAGCTGCTCGCGG
GCGACTTCATGACGGCGCTGCCCGCCGATGCGCCCGCGCTCGACGCGCTCGCGGTGGCGCGGGTGTCGCCGGGGCTGTCG
TCGGATCTGCTCGAGCAGCGCCCGGACATCCGGCAGGCCGAGCAGCGCCTCGTCGCGGCGAACGCGAACATCGGCGCGGC
GCGCGCGGCGTTCTTCCCGCGCATCGCGCTCACGACCGACGTCGGCTCGGTGAGCGACGCGTTCTCGGGACTCTTCTCGG
CCGGCTCGAGCGTGTGGACGTTCGCGCCGCGGCTCACGCTGCCGATTTTCGCGGGCGGGCGCAATCGTGCGAATCTCGAC
GTGGCGGACGCGCGCAAGCATATCGCGGTCGCCGAATACGAGAAGACGATCCAGACGGCGTTTCGCGAAGTCGCCGATGC
GCTCGCGGCGCGCGATCAGATCGACGCGCAGCTTGCCGCGCAGCAGGCGGTCTACGGTGCGGACGCCGAGCGGCTGCGGC
TCGCGCAGCGGCGCTACGACAGCGGCGTGGCGAGCTATCTCGAGCTGCTCGACGCGCAGCGCAGCACGTTCGAGTCCGGG
CAGGAGCTGATTCGCCTGAAGCAGCTGCGGCTCACGAACGCGATCACGCTGTATCGCGCGCTGGGCGGGGGCTGGTCGCG
CGCCGGGTGCGGCGGCGATGGGTGTGCTTGA

Upstream 100 bases:

>100_bases
TCGCGATCTTCCTCGTCCCGCTCTTTTTCGTCTGCGTCGGGCGCGTGTTCGACGTCGTTCCGCGCCGCCGAGGCGGCGCG
CAAGCGGCACTGGAGGCCAA

Downstream 100 bases:

>100_bases
GCGCTTGAGCGCTTGAGCGCGCTGTGCCTCGTGCCTCGTGCCTCGTGCCTCGTGCCTCGTGCCTCGTGCCTCGTGCCTCG
TGCCTCGTGCCTCGTGCGAT

Product: multidrug efflux system putative membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 516; Mature: 516

Protein sequence:

>516_residues
MKAMMKPRARRRGARAARRPNGPRAWPLAVAAALVAGCTLAPRYERPAAPVPAHYWSTATGAPREAGPAAAGGPRAMPDA
RRGDAGRDARDARLDDWRAYFTDPALRALIDAALANNRDLRIATLRIQEARGLYGVARADRLPSIDGSLGYERTRLYDPV
LRESATSSLYRASVGVSAFEIDLFGRVKSLSDAALAEYFATAEAQRAARISLIAEVASAYVTERALVDQLGLAERTLAAR
DAAYALTQRRYAAGTSTAIELRTAEMLVASARASKAALEREHTQAASALKLLAGDFMTALPADAPALDALAVARVSPGLS
SDLLEQRPDIRQAEQRLVAANANIGAARAAFFPRIALTTDVGSVSDAFSGLFSAGSSVWTFAPRLTLPIFAGGRNRANLD
VADARKHIAVAEYEKTIQTAFREVADALAARDQIDAQLAAQQAVYGADAERLRLAQRRYDSGVASYLELLDAQRSTFESG
QELIRLKQLRLTNAITLYRALGGGWSRAGCGGDGCA

Sequences:

>Translated_516_residues
MKAMMKPRARRRGARAARRPNGPRAWPLAVAAALVAGCTLAPRYERPAAPVPAHYWSTATGAPREAGPAAAGGPRAMPDA
RRGDAGRDARDARLDDWRAYFTDPALRALIDAALANNRDLRIATLRIQEARGLYGVARADRLPSIDGSLGYERTRLYDPV
LRESATSSLYRASVGVSAFEIDLFGRVKSLSDAALAEYFATAEAQRAARISLIAEVASAYVTERALVDQLGLAERTLAAR
DAAYALTQRRYAAGTSTAIELRTAEMLVASARASKAALEREHTQAASALKLLAGDFMTALPADAPALDALAVARVSPGLS
SDLLEQRPDIRQAEQRLVAANANIGAARAAFFPRIALTTDVGSVSDAFSGLFSAGSSVWTFAPRLTLPIFAGGRNRANLD
VADARKHIAVAEYEKTIQTAFREVADALAARDQIDAQLAAQQAVYGADAERLRLAQRRYDSGVASYLELLDAQRSTFESG
QELIRLKQLRLTNAITLYRALGGGWSRAGCGGDGCA
>Mature_516_residues
MKAMMKPRARRRGARAARRPNGPRAWPLAVAAALVAGCTLAPRYERPAAPVPAHYWSTATGAPREAGPAAAGGPRAMPDA
RRGDAGRDARDARLDDWRAYFTDPALRALIDAALANNRDLRIATLRIQEARGLYGVARADRLPSIDGSLGYERTRLYDPV
LRESATSSLYRASVGVSAFEIDLFGRVKSLSDAALAEYFATAEAQRAARISLIAEVASAYVTERALVDQLGLAERTLAAR
DAAYALTQRRYAAGTSTAIELRTAEMLVASARASKAALEREHTQAASALKLLAGDFMTALPADAPALDALAVARVSPGLS
SDLLEQRPDIRQAEQRLVAANANIGAARAAFFPRIALTTDVGSVSDAFSGLFSAGSSVWTFAPRLTLPIFAGGRNRANLD
VADARKHIAVAEYEKTIQTAFREVADALAARDQIDAQLAAQQAVYGADAERLRLAQRRYDSGVASYLELLDAQRSTFESG
QELIRLKQLRLTNAITLYRALGGGWSRAGCGGDGCA

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=482, Percent_Identity=41.0788381742739, Blast_Score=347, Evalue=7e-97,
Organism=Escherichia coli, GI2367348, Length=433, Percent_Identity=26.7898383371824, Blast_Score=99, Evalue=8e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 55140; Mature: 55140

Theoretical pI: Translated: 10.06; Mature: 10.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAMMKPRARRRGARAARRPNGPRAWPLAVAAALVAGCTLAPRYERPAAPVPAHYWSTAT
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCC
GAPREAGPAAAGGPRAMPDARRGDAGRDARDARLDDWRAYFTDPALRALIDAALANNRDL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCE
RIATLRIQEARGLYGVARADRLPSIDGSLGYERTRLYDPVLRESATSSLYRASVGVSAFE
EEEEEEEHHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IDLFGRVKSLSDAALAEYFATAEAQRAARISLIAEVASAYVTERALVDQLGLAERTLAAR
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
DAAYALTQRRYAAGTSTAIELRTAEMLVASARASKAALEREHTQAASALKLLAGDFMTAL
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PADAPALDALAVARVSPGLSSDLLEQRPDIRQAEQRLVAANANIGAARAAFFPRIALTTD
CCCCCHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHCCCEEEECC
VGSVSDAFSGLFSAGSSVWTFAPRLTLPIFAGGRNRANLDVADARKHIAVAEYEKTIQTA
CCHHHHHHHHHHHCCCCCEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FREVADALAARDQIDAQLAAQQAVYGADAERLRLAQRRYDSGVASYLELLDAQRSTFESG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QELIRLKQLRLTNAITLYRALGGGWSRAGCGGDGCA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKAMMKPRARRRGARAARRPNGPRAWPLAVAAALVAGCTLAPRYERPAAPVPAHYWSTAT
CCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHCCCCC
GAPREAGPAAAGGPRAMPDARRGDAGRDARDARLDDWRAYFTDPALRALIDAALANNRDL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCE
RIATLRIQEARGLYGVARADRLPSIDGSLGYERTRLYDPVLRESATSSLYRASVGVSAFE
EEEEEEEHHHCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IDLFGRVKSLSDAALAEYFATAEAQRAARISLIAEVASAYVTERALVDQLGLAERTLAAR
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
DAAYALTQRRYAAGTSTAIELRTAEMLVASARASKAALEREHTQAASALKLLAGDFMTAL
HHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PADAPALDALAVARVSPGLSSDLLEQRPDIRQAEQRLVAANANIGAARAAFFPRIALTTD
CCCCCHHHHHHHHHCCCCCCHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHCCCEEEECC
VGSVSDAFSGLFSAGSSVWTFAPRLTLPIFAGGRNRANLDVADARKHIAVAEYEKTIQTA
CCHHHHHHHHHHHCCCCCEEECCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHH
FREVADALAARDQIDAQLAAQQAVYGADAERLRLAQRRYDSGVASYLELLDAQRSTFESG
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QELIRLKQLRLTNAITLYRALGGGWSRAGCGGDGCA
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]