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The map label for this gene is phaZ1 [H]

Identifier: 53719251

GI number: 53719251

Start: 1880385

End: 1881473

Strand: Reverse

Name: phaZ1 [H]

Synonym: BPSL1623

Alternate gene names: 53719251

Gene position: 1881473-1880385 (Counterclockwise)

Preceding gene: 53719252

Following gene: 53719248

Centisome position: 46.18

GC content: 67.03

Gene sequence:

>1089_bases
ATGCGCAGAAAAAAGTCCAGCATCTGGCTACCGTACTTCGACCTGCTCTCCCTGACGCTGCGCGCGCGCCCTCGGCAAAA
GGTCGCCAAACCCAAGCCGTCCGTCACGCGCCCCACAAGCGCCGCGAAGACTCGGAGCACGAAACGCACGCGCGCCGCGC
ATGCCGGGCGAACGTCGGCGGACAACGCGCGCGGCACCTGGCTGCGCTCGTTTCACTCCGCCCGCCCCGCGGCGGGACGC
TTGATCAATCATCTCGCGTATGCGCTGTATCTGCCCGCCGCACCGGCCGCGGCGGCAAGCATGCCCGCCGTCGTCATGTT
GCACGGATGCAAACAGACCGCGGAGTCGTTCGCCACCGGCACGCGCATTTGCGATCTCGCGCAGCGGGCGGGGTTCGCCG
TGTTGCTTCCCGAGCAGGCCAAGACTTCGCATTCTCACCGGTGCTGGAACTGGCACGGCGATTCATCGCAGTCCGAAGCG
CCGGCCGTCGCCTCGCTCGTCGACGCGATCATTCGGCAGTACGGTTTCGACCGCGAGCGAATCTATCTGGCGGGCCTCTC
CGCGGGAGCCGGCCTGGCGGCGGGACTCGCGATGCGCTATCCCGAGCTCTTCGCGGCCGTCGGCCTGCACTCCGGCCCGG
TCTTCGGCGCGCCCTCGTCCACCCTCGCGGCGATGAGCCTGATGCGCGGCGGCAGCCGGGAAGATCCGCTACGCGTCATC
GAAAACTGCGTCGACGTTTCGGATCATCCCGGCATGCCCGCACTCATCGTCCACGGTGAACACGATACGGTGGTGGCGAA
GCAGAACGCGATGCAACTGGGTCTCGAGTTCGCGCGAATCAATCGGCTCATCGACGGGCAGGGCACACTGCGCGTGGGCG
AGCAACACGTCTACAGCCGCAAGGGCGTCGACTATACCGACTATCTCAAGTCCGGGCGGCTCGTCGTCAGGGTGTGCATC
GTTCACGGGCTGCGGCACGCATGGAGTGGCGGCGATCCGCGCGAAGCATTCCATTCCGCCACCGGGCCGGATGCCACCGC
GATGTTCTGGCATTTCTTTCGGCCGCGGCGTCGCAAGCGGGCACAGTGA

Upstream 100 bases:

>100_bases
GGCTGCGAACGTCCTCCGAAGCGCCGCCGAGCCACGTTGCGGCGTCCAACGCGCCGACACGGCGGCGAAGTGCAATCGAA
CCCCTTGTCTGCCTTCCGAT

Downstream 100 bases:

>100_bases
ATGAACGCGCCACACGGACGGCCTCGCGACCGTGACGGCGCGCCCGACGCTGCGCCGGCCGCGCCGCTCCGTATCCGCGC
CGATATGACGGCGATGCCCG

Product: hypothetical protein

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 362; Mature: 362

Protein sequence:

>362_residues
MRRKKSSIWLPYFDLLSLTLRARPRQKVAKPKPSVTRPTSAAKTRSTKRTRAAHAGRTSADNARGTWLRSFHSARPAAGR
LINHLAYALYLPAAPAAAASMPAVVMLHGCKQTAESFATGTRICDLAQRAGFAVLLPEQAKTSHSHRCWNWHGDSSQSEA
PAVASLVDAIIRQYGFDRERIYLAGLSAGAGLAAGLAMRYPELFAAVGLHSGPVFGAPSSTLAAMSLMRGGSREDPLRVI
ENCVDVSDHPGMPALIVHGEHDTVVAKQNAMQLGLEFARINRLIDGQGTLRVGEQHVYSRKGVDYTDYLKSGRLVVRVCI
VHGLRHAWSGGDPREAFHSATGPDATAMFWHFFRPRRRKRAQ

Sequences:

>Translated_362_residues
MRRKKSSIWLPYFDLLSLTLRARPRQKVAKPKPSVTRPTSAAKTRSTKRTRAAHAGRTSADNARGTWLRSFHSARPAAGR
LINHLAYALYLPAAPAAAASMPAVVMLHGCKQTAESFATGTRICDLAQRAGFAVLLPEQAKTSHSHRCWNWHGDSSQSEA
PAVASLVDAIIRQYGFDRERIYLAGLSAGAGLAAGLAMRYPELFAAVGLHSGPVFGAPSSTLAAMSLMRGGSREDPLRVI
ENCVDVSDHPGMPALIVHGEHDTVVAKQNAMQLGLEFARINRLIDGQGTLRVGEQHVYSRKGVDYTDYLKSGRLVVRVCI
VHGLRHAWSGGDPREAFHSATGPDATAMFWHFFRPRRRKRAQ
>Mature_362_residues
MRRKKSSIWLPYFDLLSLTLRARPRQKVAKPKPSVTRPTSAAKTRSTKRTRAAHAGRTSADNARGTWLRSFHSARPAAGR
LINHLAYALYLPAAPAAAASMPAVVMLHGCKQTAESFATGTRICDLAQRAGFAVLLPEQAKTSHSHRCWNWHGDSSQSEA
PAVASLVDAIIRQYGFDRERIYLAGLSAGAGLAAGLAMRYPELFAAVGLHSGPVFGAPSSTLAAMSLMRGGSREDPLRVI
ENCVDVSDHPGMPALIVHGEHDTVVAKQNAMQLGLEFARINRLIDGQGTLRVGEQHVYSRKGVDYTDYLKSGRLVVRVCI
VHGLRHAWSGGDPREAFHSATGPDATAMFWHFFRPRRRKRAQ

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 39545; Mature: 39545

Theoretical pI: Translated: 11.08; Mature: 11.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRKKSSIWLPYFDLLSLTLRARPRQKVAKPKPSVTRPTSAAKTRSTKRTRAAHAGRTSA
CCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
DNARGTWLRSFHSARPAAGRLINHLAYALYLPAAPAAAASMPAVVMLHGCKQTAESFATG
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHH
TRICDLAQRAGFAVLLPEQAKTSHSHRCWNWHGDSSQSEAPAVASLVDAIIRQYGFDRER
HHHHHHHHHCCEEEEECCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCE
IYLAGLSAGAGLAAGLAMRYPELFAAVGLHSGPVFGAPSSTLAAMSLMRGGSREDPLRVI
EEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHH
ENCVDVSDHPGMPALIVHGEHDTVVAKQNAMQLGLEFARINRLIDGQGTLRVGEQHVYSR
HHHHCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHH
KGVDYTDYLKSGRLVVRVCIVHGLRHAWSGGDPREAFHSATGPDATAMFWHFFRPRRRKR
CCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHCHHHHCC
AQ
CC
>Mature Secondary Structure
MRRKKSSIWLPYFDLLSLTLRARPRQKVAKPKPSVTRPTSAAKTRSTKRTRAAHAGRTSA
CCCCCCCCCCCHHHHHHHHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCC
DNARGTWLRSFHSARPAAGRLINHLAYALYLPAAPAAAASMPAVVMLHGCKQTAESFATG
CCCCHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHHHHHHHH
TRICDLAQRAGFAVLLPEQAKTSHSHRCWNWHGDSSQSEAPAVASLVDAIIRQYGFDRER
HHHHHHHHHCCEEEEECCHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCE
IYLAGLSAGAGLAAGLAMRYPELFAAVGLHSGPVFGAPSSTLAAMSLMRGGSREDPLRVI
EEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCCCCCHHHHH
ENCVDVSDHPGMPALIVHGEHDTVVAKQNAMQLGLEFARINRLIDGQGTLRVGEQHVYSR
HHHHCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHH
KGVDYTDYLKSGRLVVRVCIVHGLRHAWSGGDPREAFHSATGPDATAMFWHFFRPRRRKR
CCCCHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHCHHHHCC
AQ
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]