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The map label for this gene is ygfZ [C]

Identifier: 53719070

GI number: 53719070

Start: 1670668

End: 1671621

Strand: Reverse

Name: ygfZ [C]

Synonym: BPSL1434

Alternate gene names: 53719070

Gene position: 1671621-1670668 (Counterclockwise)

Preceding gene: 53719081

Following gene: 53719069

Centisome position: 41.03

GC content: 72.75

Gene sequence:

>954_bases
GTGCTCGAGCGCGGCGCGTTCGCGGCGCTCGAGCAATTCGGCATCGTCGACGTGACGGGCGCCGACGCCGCGACGTTTCT
GCACGGCCAGTTGACGAACGACGTCGAACATCTCGACGCCGCGAGCGCGCGCCTGGCCGGCTACTGCTCGCCGAAGGGGC
GCCTGCTCGCGTCGTTCCTCGCATGGCGCGCCGGCCACGACGTGCGCCTGCTCGTATCGAAGGACGTGCAGCCCGCGGTG
CAAAAGCGCCTGTCGATGTTCGTGCTGCGCGCGAAGGCGAAGCTCGCCGACGCGAGCGGCGCGCTCGTCGCGATCGGCTT
CGCGGGCGACGTGCGCGCCGCGCTGTCGGGCATCTTCGACGCCCTGCCCGACGGCATCCACACGAAAGTCGACGCGCCGG
CCGGCGCGCTCATGCGGCTGCCCGACGCGGCGGGCCGCGCGCGCTACCTGTGGATCGCGACGCGCGCCGAGTTCGACGCG
CGCCTGCCCGCGCTCGAAGCCGCGCTGCCGCGCGTGTCGGCCGCCGTCTGGGATTGGCTCGACGTGCGCGCGGGCGAGCC
GCGCATCACGCAGCCGGCCGTCGAGCAATTCGTTCCGCAGATGGTCAACTTCGACGTGATCGGCGGCGTCAACTTCCGCA
AGGGCTGCTATCCGGGCCAGGAAGTCGTCGCGCGCAGCCAGTACCGGGGCACGATCAAGCGCCGCACCGCGCTCGCGCAC
GTCGCGGCCGGCACCGACGCCGCGCATGCGGGCGTCGAGCTCTATCATTCGGACGATCCGGGCCAGCCGTGCGGGATGAT
CGTGAACGCGGCGGCGGCGCCCGAAGGCGGCGTCGACGCGCTCGTCGAGATCAAGCTCGCCGCGCTCGGGAACGGATCGG
TCCACCTGGCGTCCGCGAGCGGCCCGACGCTCGCGTTCCTGCCGCTTCCCTACGCGCTGCCCGTCGAAGCCTGA

Upstream 100 bases:

>100_bases
TCATTCCATGAGCACACCGATCGCCTCCCCGGCCGCGCAGGCCATCGCTCCCACCGCGCCGCCGACGCTGCCCCGTCCGA
GCGCCGACGATTTCGCCGCC

Downstream 100 bases:

>100_bases
CGGCCGCGCGCCGCCGGAGCGCGCGCGCCGCGGCGCGCCGGCCGGCCTCACCCAGCGAGGATATCGCCCGATGTGCCTGA
TCGTATTCGATTGGCAGCCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MLERGAFAALEQFGIVDVTGADAATFLHGQLTNDVEHLDAASARLAGYCSPKGRLLASFLAWRAGHDVRLLVSKDVQPAV
QKRLSMFVLRAKAKLADASGALVAIGFAGDVRAALSGIFDALPDGIHTKVDAPAGALMRLPDAAGRARYLWIATRAEFDA
RLPALEAALPRVSAAVWDWLDVRAGEPRITQPAVEQFVPQMVNFDVIGGVNFRKGCYPGQEVVARSQYRGTIKRRTALAH
VAAGTDAAHAGVELYHSDDPGQPCGMIVNAAAAPEGGVDALVEIKLAALGNGSVHLASASGPTLAFLPLPYALPVEA

Sequences:

>Translated_317_residues
MLERGAFAALEQFGIVDVTGADAATFLHGQLTNDVEHLDAASARLAGYCSPKGRLLASFLAWRAGHDVRLLVSKDVQPAV
QKRLSMFVLRAKAKLADASGALVAIGFAGDVRAALSGIFDALPDGIHTKVDAPAGALMRLPDAAGRARYLWIATRAEFDA
RLPALEAALPRVSAAVWDWLDVRAGEPRITQPAVEQFVPQMVNFDVIGGVNFRKGCYPGQEVVARSQYRGTIKRRTALAH
VAAGTDAAHAGVELYHSDDPGQPCGMIVNAAAAPEGGVDALVEIKLAALGNGSVHLASASGPTLAFLPLPYALPVEA
>Mature_317_residues
MLERGAFAALEQFGIVDVTGADAATFLHGQLTNDVEHLDAASARLAGYCSPKGRLLASFLAWRAGHDVRLLVSKDVQPAV
QKRLSMFVLRAKAKLADASGALVAIGFAGDVRAALSGIFDALPDGIHTKVDAPAGALMRLPDAAGRARYLWIATRAEFDA
RLPALEAALPRVSAAVWDWLDVRAGEPRITQPAVEQFVPQMVNFDVIGGVNFRKGCYPGQEVVARSQYRGTIKRRTALAH
VAAGTDAAHAGVELYHSDDPGQPCGMIVNAAAAPEGGVDALVEIKLAALGNGSVHLASASGPTLAFLPLPYALPVEA

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=307, Percent_Identity=28.3387622149837, Blast_Score=112, Evalue=4e-26,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 33272; Mature: 33272

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLERGAFAALEQFGIVDVTGADAATFLHGQLTNDVEHLDAASARLAGYCSPKGRLLASFL
CCCCHHHHHHHHCCCEEECCCCHHHEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
AWRAGHDVRLLVSKDVQPAVQKRLSMFVLRAKAKLADASGALVAIGFAGDVRAALSGIFD
HHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHH
ALPDGIHTKVDAPAGALMRLPDAAGRARYLWIATRAEFDARLPALEAALPRVSAAVWDWL
HCCCCCCEECCCCCCCEEECCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
DVRAGEPRITQPAVEQFVPQMVNFDVIGGVNFRKGCYPGQEVVARSQYRGTIKRRTALAH
HCCCCCCCCCHHHHHHHHHHHHCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VAAGTDAAHAGVELYHSDDPGQPCGMIVNAAAAPEGGVDALVEIKLAALGNGSVHLASAS
HHCCCCHHHCCEEEEECCCCCCCCCCEEEECCCCCCCCCEEEEEEEEEECCCCEEEEECC
GPTLAFLPLPYALPVEA
CCEEEEEECCCCCCCCC
>Mature Secondary Structure
MLERGAFAALEQFGIVDVTGADAATFLHGQLTNDVEHLDAASARLAGYCSPKGRLLASFL
CCCCHHHHHHHHCCCEEECCCCHHHEECCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
AWRAGHDVRLLVSKDVQPAVQKRLSMFVLRAKAKLADASGALVAIGFAGDVRAALSGIFD
HHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHH
ALPDGIHTKVDAPAGALMRLPDAAGRARYLWIATRAEFDARLPALEAALPRVSAAVWDWL
HCCCCCCEECCCCCCCEEECCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHH
DVRAGEPRITQPAVEQFVPQMVNFDVIGGVNFRKGCYPGQEVVARSQYRGTIKRRTALAH
HCCCCCCCCCHHHHHHHHHHHHCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
VAAGTDAAHAGVELYHSDDPGQPCGMIVNAAAAPEGGVDALVEIKLAALGNGSVHLASAS
HHCCCCHHHCCEEEEECCCCCCCCCCEEEECCCCCCCCCEEEEEEEEEECCCCEEEEECC
GPTLAFLPLPYALPVEA
CCEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA