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The map label for this gene is btuF [H]

Identifier: 53718625

GI number: 53718625

Start: 1141238

End: 1142170

Strand: Reverse

Name: btuF [H]

Synonym: BPSL0983

Alternate gene names: 53718625

Gene position: 1142170-1141238 (Counterclockwise)

Preceding gene: 53718626

Following gene: 53718624

Centisome position: 28.03

GC content: 72.45

Gene sequence:

>933_bases
ATGCGCCCCGCCCTGCGCCGCGCCGCACGCATCGCACTCGCCGCCCTCTTCTGCGCGAGCGCATGCGCGCATGCGCTCGC
CGCCGCGCATGCGGACATCGCCGTCACCGACGACGCCGGGCACGCGATCACGCTCGCCGCGCCCGCACGGCGCGTCGTGA
GCCTCGCGCCGCACGTGACCGAGCTGATCTATGCGGCGGGCGGCGGCGCGAAGCTCGTCGGCGCGGTGTCGTACAGCGAC
TACCCGCCCGCCGCGAAGGCGATTGCGCGCGTCGGCTCGAACAAGGCGCTCGATCTCGAACGGATCGCGGCGCTCAAGCC
CGATCTGATCGTCGTCTGGCGCCACGGCAACGCCGAGCACGAGACCGAGCGGCTGCGCGCGCTCGGCATTCCGCTTTACT
TCAGCGAGCCACGGCATCTCGACGATGTCGCCGCATCGCTCGACAAGCTCGGCCTGCTGCTCGGCACGCACGAGATCGCG
TCGGCCGCGGCCGACGCGTACCGGCGGCGAATCGCGCAGCTGCGCGCGCGCTACGCGGACAAGCCGCCCGTCACGGTGTT
CTTCCAGGCGTGGGACAAACCGCTCATCACGCTGAACGGCGATCACATCGTGAGCGACGTGATCGCGCTGTGCGGCGGGC
GCAACGTGTTCGCGCGGCTGCAGCCGGTCGCGCCGTCCGTCACCGACGAAGCGGTGCTCGCGGCAAATCCTGAGGCGATC
GTGACGACGGCGGCGGGCGCGAGCCCGACGCGCGACGCACTGCCCGACTTCACGCGCTGGCGCGCATGGCCGAAGCTGAC
CGCGGTCGCGCGGGGCAATCTGTTCGCGATCGACGGCGATCTGCTGACGCGGCCCGCGCCGCGCATCGCGCAGGGCGCCG
AGCAACTGTGCCGGGATCTCGACGTCGCGCGCGCGAGACGGCCGAAGCCGTGA

Upstream 100 bases:

>100_bases
ACGACTGGCGGCGGCTCGAACGCACGCTCGCCGAGTGCGTGCCGACGCTAGCGGCCGCAGCCGCGCATCCTTCCGAATCG
ACCACACGGGATTGACACCG

Downstream 100 bases:

>100_bases
GCGCGGCGCGCCGGCGAGCGCCCGCCGCTCGCCGCCCGCGTCGAAACGCGCGGGCGAACCGGGGCCGCGGCGGCCCGCGC
ACGCCGATGTCAGGGGTACC

Product: putative vitamin B12 transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MRPALRRAARIALAALFCASACAHALAAAHADIAVTDDAGHAITLAAPARRVVSLAPHVTELIYAAGGGAKLVGAVSYSD
YPPAAKAIARVGSNKALDLERIAALKPDLIVVWRHGNAEHETERLRALGIPLYFSEPRHLDDVAASLDKLGLLLGTHEIA
SAAADAYRRRIAQLRARYADKPPVTVFFQAWDKPLITLNGDHIVSDVIALCGGRNVFARLQPVAPSVTDEAVLAANPEAI
VTTAAGASPTRDALPDFTRWRAWPKLTAVARGNLFAIDGDLLTRPAPRIAQGAEQLCRDLDVARARRPKP

Sequences:

>Translated_310_residues
MRPALRRAARIALAALFCASACAHALAAAHADIAVTDDAGHAITLAAPARRVVSLAPHVTELIYAAGGGAKLVGAVSYSD
YPPAAKAIARVGSNKALDLERIAALKPDLIVVWRHGNAEHETERLRALGIPLYFSEPRHLDDVAASLDKLGLLLGTHEIA
SAAADAYRRRIAQLRARYADKPPVTVFFQAWDKPLITLNGDHIVSDVIALCGGRNVFARLQPVAPSVTDEAVLAANPEAI
VTTAAGASPTRDALPDFTRWRAWPKLTAVARGNLFAIDGDLLTRPAPRIAQGAEQLCRDLDVARARRPKP
>Mature_310_residues
MRPALRRAARIALAALFCASACAHALAAAHADIAVTDDAGHAITLAAPARRVVSLAPHVTELIYAAGGGAKLVGAVSYSD
YPPAAKAIARVGSNKALDLERIAALKPDLIVVWRHGNAEHETERLRALGIPLYFSEPRHLDDVAASLDKLGLLLGTHEIA
SAAADAYRRRIAQLRARYADKPPVTVFFQAWDKPLITLNGDHIVSDVIALCGGRNVFARLQPVAPSVTDEAVLAANPEAI
VTTAAGASPTRDALPDFTRWRAWPKLTAVARGNLFAIDGDLLTRPAPRIAQGAEQLCRDLDVARARRPKP

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786353, Length=252, Percent_Identity=37.3015873015873, Blast_Score=158, Evalue=4e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 33009; Mature: 33009

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPALRRAARIALAALFCASACAHALAAAHADIAVTDDAGHAITLAAPARRVVSLAPHVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECHHHHHHHHHHHHH
ELIYAAGGGAKLVGAVSYSDYPPAAKAIARVGSNKALDLERIAALKPDLIVVWRHGNAEH
HHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEECCCCCHH
ETERLRALGIPLYFSEPRHLDDVAASLDKLGLLLGTHEIASAAADAYRRRIAQLRARYAD
HHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KPPVTVFFQAWDKPLITLNGDHIVSDVIALCGGRNVFARLQPVAPSVTDEAVLAANPEAI
CCCEEEEEECCCCCEEEECCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEECCCCEE
VTTAAGASPTRDALPDFTRWRAWPKLTAVARGNLFAIDGDLLTRPAPRIAQGAEQLCRDL
EEECCCCCCCHHCCCCHHHHHCCHHHHHEECCCEEEECCHHHCCCCHHHHHHHHHHHHHH
DVARARRPKP
HHHHHCCCCC
>Mature Secondary Structure
MRPALRRAARIALAALFCASACAHALAAAHADIAVTDDAGHAITLAAPARRVVSLAPHVT
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEECHHHHHHHHHHHHH
ELIYAAGGGAKLVGAVSYSDYPPAAKAIARVGSNKALDLERIAALKPDLIVVWRHGNAEH
HHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEECCCCCHH
ETERLRALGIPLYFSEPRHLDDVAASLDKLGLLLGTHEIASAAADAYRRRIAQLRARYAD
HHHHHHHHCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KPPVTVFFQAWDKPLITLNGDHIVSDVIALCGGRNVFARLQPVAPSVTDEAVLAANPEAI
CCCEEEEEECCCCCEEEECCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCEEEECCCCEE
VTTAAGASPTRDALPDFTRWRAWPKLTAVARGNLFAIDGDLLTRPAPRIAQGAEQLCRDL
EEECCCCCCCHHCCCCHHHHHCCHHHHHEECCCEEEECCHHHCCCCHHHHHHHHHHHHHH
DVARARRPKP
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA