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The map label for this gene is ytzG [H]

Identifier: 53718572

GI number: 53718572

Start: 1082519

End: 1083241

Strand: Reverse

Name: ytzG [H]

Synonym: BPSL0930

Alternate gene names: 53718572

Gene position: 1083241-1082519 (Counterclockwise)

Preceding gene: 53718574

Following gene: 53718571

Centisome position: 26.59

GC content: 69.43

Gene sequence:

>723_bases
ATGGATCTCGAAAGCATTCTGTTCACCCAAGGCTTCGGTTCGCGCCGCCAGTGCCGCGCGCTCGTCGAAGCGGGACACGT
CGCCGTCGGCGGCGCGACGTGCGCCGACGCGCACGCATCGTTCGACACCACGCAGCTCGTGTTCGAAGTCGACGGCGTCG
CCTGGCCGTACCGCGCGCGCGCTTACGTCGCGCTCAACAAGCCGGCCGGCTACGAATGCTCGCGCGAGCCGCAGCATCAT
TCGAGCGTGTTCGCGCTGCTGCCGCCGCAGTTCGCCGAGCGTGGCGTCCAGTGCGTCGGCCGCCTCGACCAGGACACGAC
GGGCCTGCTGCTCCTGTCCGACGACGGCCAGTTCGTCCACGCGTACACGTCGCCGAAACGCAAGGTGCCGAAAACCTATG
TCGCGACGGTGCGCCACCCGCTCGACGACGCGCAGCTCGACGCGCTGCGCGCGGGCGTGCTGCTGCACGGCGAGCCGAAA
CCGGTCGCCGCGCTCGCCGCCGTTGCCCGAGGAGAACGACTCCTCGAAATGACGATCGCCGAAGGGAAATACCACCAGGT
GAAGCGCATGGTCGCCGCCGCGAGCAACCGCGTCGAGGCGTTGCACCGCTCGCGCATCGGCGGCTTCGCGCTGCCCGAGG
ATCTCGCCGAAGGCGCGTGGCGCTGGCTCGACGAACGCGATCTCGCCGCCTTGCGCGACACGGCGGAAACCCTGTCAGGG
TAA

Upstream 100 bases:

>100_bases
ACGACCGCGGGGCCCCGAGCTTAACAGCCCGCGCGCGCCTGCGCTCGGGCGCGCCGCTTGCCCGAGCCGCGCTTGATGCG
CTTGGTACAATGCGCGCGCC

Downstream 100 bases:

>100_bases
GCTCGGAGGGCCGCCGTTCGGCGCGCCGAGCCATGCTGCCGCGCAGCATGGCAGCGTCATCAAGCCCCGCTATACTCGAA
CCAAACCACAATACGTTCCA

Product: putative ribosomal small subunit pseudouridine synthase A

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MDLESILFTQGFGSRRQCRALVEAGHVAVGGATCADAHASFDTTQLVFEVDGVAWPYRARAYVALNKPAGYECSREPQHH
SSVFALLPPQFAERGVQCVGRLDQDTTGLLLLSDDGQFVHAYTSPKRKVPKTYVATVRHPLDDAQLDALRAGVLLHGEPK
PVAALAAVARGERLLEMTIAEGKYHQVKRMVAAASNRVEALHRSRIGGFALPEDLAEGAWRWLDERDLAALRDTAETLSG

Sequences:

>Translated_240_residues
MDLESILFTQGFGSRRQCRALVEAGHVAVGGATCADAHASFDTTQLVFEVDGVAWPYRARAYVALNKPAGYECSREPQHH
SSVFALLPPQFAERGVQCVGRLDQDTTGLLLLSDDGQFVHAYTSPKRKVPKTYVATVRHPLDDAQLDALRAGVLLHGEPK
PVAALAAVARGERLLEMTIAEGKYHQVKRMVAAASNRVEALHRSRIGGFALPEDLAEGAWRWLDERDLAALRDTAETLSG
>Mature_240_residues
MDLESILFTQGFGSRRQCRALVEAGHVAVGGATCADAHASFDTTQLVFEVDGVAWPYRARAYVALNKPAGYECSREPQHH
SSVFALLPPQFAERGVQCVGRLDQDTTGLLLLSDDGQFVHAYTSPKRKVPKTYVATVRHPLDDAQLDALRAGVLLHGEPK
PVAALAAVARGERLLEMTIAEGKYHQVKRMVAAASNRVEALHRSRIGGFALPEDLAEGAWRWLDERDLAALRDTAETLSG

Specific function: Responsible For Synthesis Of Pseudouridine From Uracil-516 In 16s Ribosomal RNA. [C]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=173, Percent_Identity=44.5086705202312, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787524, Length=243, Percent_Identity=32.9218106995885, Blast_Score=107, Evalue=6e-25,
Organism=Escherichia coli, GI1790453, Length=242, Percent_Identity=27.2727272727273, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081838, Length=146, Percent_Identity=33.5616438356164, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70

Molecular weight: Translated: 26156; Mature: 26156

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLESILFTQGFGSRRQCRALVEAGHVAVGGATCADAHASFDTTQLVFEVDGVAWPYRAR
CCHHHHHHCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCE
AYVALNKPAGYECSREPQHHSSVFALLPPQFAERGVQCVGRLDQDTTGLLLLSDDGQFVH
EEEEECCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEE
AYTSPKRKVPKTYVATVRHPLDDAQLDALRAGVLLHGEPKPVAALAAVARGERLLEMTIA
EECCCCCCCCHHHHHHHHCCCCHHHHHHHHHCEEEECCCCHHHHHHHHHHCCCEEEEECC
EGKYHQVKRMVAAASNRVEALHRSRIGGFALPEDLAEGAWRWLDERDLAALRDTAETLSG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLESILFTQGFGSRRQCRALVEAGHVAVGGATCADAHASFDTTQLVFEVDGVAWPYRAR
CCHHHHHHCCCCCCHHHHHHHHHCCCEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCCE
AYVALNKPAGYECSREPQHHSSVFALLPPQFAERGVQCVGRLDQDTTGLLLLSDDGQFVH
EEEEECCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCCEEEEECCCCCEEE
AYTSPKRKVPKTYVATVRHPLDDAQLDALRAGVLLHGEPKPVAALAAVARGERLLEMTIA
EECCCCCCCCHHHHHHHHCCCCHHHHHHHHHCEEEECCCCHHHHHHHHHHCCCEEEEECC
EGKYHQVKRMVAAASNRVEALHRSRIGGFALPEDLAEGAWRWLDERDLAALRDTAETLSG
CCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]