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The map label for this gene is glyS [H]

Identifier: 53718310

GI number: 53718310

Start: 757548

End: 759647

Strand: Reverse

Name: glyS [H]

Synonym: BPSL0667

Alternate gene names: 53718310

Gene position: 759647-757548 (Counterclockwise)

Preceding gene: 53718311

Following gene: 53718309

Centisome position: 18.64

GC content: 69.9

Gene sequence:

>2100_bases
ATGACGCAAAACCATTCCGCATCCCTGCTCGTCGAACTGCTGACCGAAGAGCTGCCGCCGAAAGCGCTCGCGCGCCTCGG
CGACGCGTTCGCCGAAGGCATCGCGCAACGCCTCGCCGCGCGCGACCTGATCGAGGGCGAGCTCGCATTCGAGCGCTACG
CGACGCCGCGCCGCCTCGCCGTCGTCGTCCAGAACGTGCGCGCCGTCGCGCCCGAACGGCAGGTTCGCGAAAAAGTGCTG
CCGGTGTCGGTCGCGCTCGACGCGCAAGGCGCGCCGAGCGCGCCGCTCGCGAAGAAGCTCGCGGCGCTCGGCCATCCGAA
CCTGTCGATCGCCGATCTCGAGCGCGCGCACGACGGCAAGGCCGAAGCGTTCTTCGTCAACTACGCGGCGCCCGGCGCGA
CGCTCGCCGACGGCCTGCAGGCCGCGCTCGACGAAACGCTCGCGAAGCTGCCGATCCCGAAGGTGATGACCTATCAGCGC
CCGGACGGCACCGATGTGCAATTCGTGCGCCCCGTGCACCGGCTGACGGTGCTGCACGGCGATCGCATCGTGCCCGTCGC
CGCATTCGGCATCGACGCGGGCGACACGACGCTCGGCCACCGCTTCCTGTCCGACGGCCTCGTCGCGATCCGGCATGCGA
ACGCCTACGCGGATACGCTGCGCGAGAAAGGCCGCGTGCTCGCGCACTTCGCGCAACGCCGGGAAACGATCCGCACGCAG
CTCGAGGCGAACGCAAACGGCGATCGGGTCGTGATGCCCGAGGCGCTTCTCGACGAAGTGACGTCGCTCGTCGAATGGCC
GGTCGTCTACCCGTGCCGCTTCGAGGACGAGTTCCTGCAAGTGCCGCAGGAATGCCTGATCCTCACGATGCAGACCAACC
AGAAGTACTTCGCGCTCACCGACGCGGCGGGCAAGCTGCGCTCGCGCTTCCTGATCGTGTCGAACATCGAGACGAAGACG
CCGGGCGAGATCGTCGAGGGCAACGAGCGCGTCGTGCGCCCGCGCCTGGCCGACGCGAAGTTCTTCTTCGAGCAGGACAA
GAAGAAGCCGCTCGCCGAGCGCGTGCCGCTCCTCGCGAACGTCGTCTATCACAACAAGCTCGGCTCGCAGCTCGCGCGCG
TCGAGCGCCTCGAAACGCTCGCGGGCGAGATCGCGCCCGCGATCGGCGCCGACGCGACGGTCGCGATTCGTGCCGCCCGC
CTCGCGAAGGCCGATCTGCTGACCGACATGGTCGGCGAGTTCCCCGAGCTGCAAGGCACGATGGGCACGTACTACGCGCG
CCACGACGGCGAGCCCGAAGACGTCGCGATCGCGTGCAGCGAGCACTACCAGCCGCGCTTCTCCGGCGACGCGCTGCCGA
CGACGCCCGTGTCGACCGCGGTCGCGCTCGCCGACAAGCTCGAGACGATCGTCGGCATCTGGGGCATCGGCCTTGCGCCG
ACGGGCGAGAAGGACCCGTTCGCGCTGCGCCGGCACGCGCTCGGCGTGCTGCGCCTGCTGCTCGAAAAGCAGTTGCCGCT
CGATCTCGTCTGGCTGCTGCGCACCGCGTACGAGCGCTTCGCGGCGGTGCCGGGCGTCGCCGAATCGACCGACGCGATCC
ATGCGTTCTTCATCGACCGCCTGCGCGGCCTGCTGCGCGAGCGCGGCTACACGGCGGGCGAAATCGACGCGGTGCTGAGC
CTGAACCCGACGCGCCTCGACGACATCGTCGCGCGCCTTAACGCGGTGCGCGAGTTCACGCGCCTCGCCGAAGCCGAGGC
GCTCGCGGCCGCGAACAAGCGGATCTCGAACATCCTGAAGAAATCGGAAAGCGGCGCGAACGGCGCGGTGCAGCCGGCGC
TCTTCGTCGAAGCGGCCGAGAAGGCGCTTGCCGAGCAGCTCGCCGACGTGACGCCGCGCGTGCAGTCGCAGCTCGAGGCG
CGCGCGTACACCGGCGCGCTCACCGCGCTCGCCGCGCTGCGCGCGCCCGTCGACGCGTTCTTCGACGGCGTGATGGTCAA
CGCCGACGATCCCGCGCTGCGGGCGAACCGGCTCGCGCTCCTGTCCGCGCTGCACCAGCAGATGAACTGCGTCGCGGACA
TCTCGAAGCTCGCCGCCTGA

Upstream 100 bases:

>100_bases
GGCCTCACCACCGACGCGCAGGAGGCCGCGCAGCCCGCATGGGCGCCGCCGCTGAAAGCCGAACGCAAGATCGATCAGGA
CTGACGAGATTCTTCACACC

Downstream 100 bases:

>100_bases
AGGCCCGGAGCCACGCGATGCCGACCAGTCTCAGCAAGAAACTCGTCGTCCTCGATCGGGACGGCGTCATCAACGTCGAT
TCGGACGCGTTCATCAAGTC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 699; Mature: 698

Protein sequence:

>699_residues
MTQNHSASLLVELLTEELPPKALARLGDAFAEGIAQRLAARDLIEGELAFERYATPRRLAVVVQNVRAVAPERQVREKVL
PVSVALDAQGAPSAPLAKKLAALGHPNLSIADLERAHDGKAEAFFVNYAAPGATLADGLQAALDETLAKLPIPKVMTYQR
PDGTDVQFVRPVHRLTVLHGDRIVPVAAFGIDAGDTTLGHRFLSDGLVAIRHANAYADTLREKGRVLAHFAQRRETIRTQ
LEANANGDRVVMPEALLDEVTSLVEWPVVYPCRFEDEFLQVPQECLILTMQTNQKYFALTDAAGKLRSRFLIVSNIETKT
PGEIVEGNERVVRPRLADAKFFFEQDKKKPLAERVPLLANVVYHNKLGSQLARVERLETLAGEIAPAIGADATVAIRAAR
LAKADLLTDMVGEFPELQGTMGTYYARHDGEPEDVAIACSEHYQPRFSGDALPTTPVSTAVALADKLETIVGIWGIGLAP
TGEKDPFALRRHALGVLRLLLEKQLPLDLVWLLRTAYERFAAVPGVAESTDAIHAFFIDRLRGLLRERGYTAGEIDAVLS
LNPTRLDDIVARLNAVREFTRLAEAEALAAANKRISNILKKSESGANGAVQPALFVEAAEKALAEQLADVTPRVQSQLEA
RAYTGALTALAALRAPVDAFFDGVMVNADDPALRANRLALLSALHQQMNCVADISKLAA

Sequences:

>Translated_699_residues
MTQNHSASLLVELLTEELPPKALARLGDAFAEGIAQRLAARDLIEGELAFERYATPRRLAVVVQNVRAVAPERQVREKVL
PVSVALDAQGAPSAPLAKKLAALGHPNLSIADLERAHDGKAEAFFVNYAAPGATLADGLQAALDETLAKLPIPKVMTYQR
PDGTDVQFVRPVHRLTVLHGDRIVPVAAFGIDAGDTTLGHRFLSDGLVAIRHANAYADTLREKGRVLAHFAQRRETIRTQ
LEANANGDRVVMPEALLDEVTSLVEWPVVYPCRFEDEFLQVPQECLILTMQTNQKYFALTDAAGKLRSRFLIVSNIETKT
PGEIVEGNERVVRPRLADAKFFFEQDKKKPLAERVPLLANVVYHNKLGSQLARVERLETLAGEIAPAIGADATVAIRAAR
LAKADLLTDMVGEFPELQGTMGTYYARHDGEPEDVAIACSEHYQPRFSGDALPTTPVSTAVALADKLETIVGIWGIGLAP
TGEKDPFALRRHALGVLRLLLEKQLPLDLVWLLRTAYERFAAVPGVAESTDAIHAFFIDRLRGLLRERGYTAGEIDAVLS
LNPTRLDDIVARLNAVREFTRLAEAEALAAANKRISNILKKSESGANGAVQPALFVEAAEKALAEQLADVTPRVQSQLEA
RAYTGALTALAALRAPVDAFFDGVMVNADDPALRANRLALLSALHQQMNCVADISKLAA
>Mature_698_residues
TQNHSASLLVELLTEELPPKALARLGDAFAEGIAQRLAARDLIEGELAFERYATPRRLAVVVQNVRAVAPERQVREKVLP
VSVALDAQGAPSAPLAKKLAALGHPNLSIADLERAHDGKAEAFFVNYAAPGATLADGLQAALDETLAKLPIPKVMTYQRP
DGTDVQFVRPVHRLTVLHGDRIVPVAAFGIDAGDTTLGHRFLSDGLVAIRHANAYADTLREKGRVLAHFAQRRETIRTQL
EANANGDRVVMPEALLDEVTSLVEWPVVYPCRFEDEFLQVPQECLILTMQTNQKYFALTDAAGKLRSRFLIVSNIETKTP
GEIVEGNERVVRPRLADAKFFFEQDKKKPLAERVPLLANVVYHNKLGSQLARVERLETLAGEIAPAIGADATVAIRAARL
AKADLLTDMVGEFPELQGTMGTYYARHDGEPEDVAIACSEHYQPRFSGDALPTTPVSTAVALADKLETIVGIWGIGLAPT
GEKDPFALRRHALGVLRLLLEKQLPLDLVWLLRTAYERFAAVPGVAESTDAIHAFFIDRLRGLLRERGYTAGEIDAVLSL
NPTRLDDIVARLNAVREFTRLAEAEALAAANKRISNILKKSESGANGAVQPALFVEAAEKALAEQLADVTPRVQSQLEAR
AYTGALTALAALRAPVDAFFDGVMVNADDPALRANRLALLSALHQQMNCVADISKLAA

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=692, Percent_Identity=44.2196531791908, Blast_Score=535, Evalue=1e-153,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 75956; Mature: 75825

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB ; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQNHSASLLVELLTEELPPKALARLGDAFAEGIAQRLAARDLIEGELAFERYATPRRLA
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VVVQNVRAVAPERQVREKVLPVSVALDAQGAPSAPLAKKLAALGHPNLSIADLERAHDGK
HHHHHHHHHCCHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCC
AEAFFVNYAAPGATLADGLQAALDETLAKLPIPKVMTYQRPDGTDVQFVRPVHRLTVLHG
CCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHEECCCCCCCHHHHHHHHHHHHHCC
DRIVPVAAFGIDAGDTTLGHRFLSDGLVAIRHANAYADTLREKGRVLAHFAQRRETIRTQ
CEEEEEEEECCCCCCHHHHHHHHHCCHHEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LEANANGDRVVMPEALLDEVTSLVEWPVVYPCRFEDEFLQVPQECLILTMQTNQKYFALT
HHCCCCCCEEECCHHHHHHHHHHHHCCCEECCCCCHHHHHCCHHHEEEEEECCCEEEEEE
DAAGKLRSRFLIVSNIETKTPGEIVEGNERVVRPRLADAKFFFEQDKKKPLAERVPLLAN
CHHHHHHHCEEEEECCCCCCCCCHHCCCCCEECCCHHHHHHHHHHHCCCCHHHHHHHHHH
VVYHNKLGSQLARVERLETLAGEIAPAIGADATVAIRAARLAKADLLTDMVGEFPELQGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCC
MGTYYARHDGEPEDVAIACSEHYQPRFSGDALPTTPVSTAVALADKLETIVGIWGIGLAP
HHHEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
TGEKDPFALRRHALGVLRLLLEKQLPLDLVWLLRTAYERFAAVPGVAESTDAIHAFFIDR
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LRGLLRERGYTAGEIDAVLSLNPTRLDDIVARLNAVREFTRLAEAEALAAANKRISNILK
HHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSESGANGAVQPALFVEAAEKALAEQLADVTPRVQSQLEARAYTGALTALAALRAPVDAF
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
FDGVMVNADDPALRANRLALLSALHQQMNCVADISKLAA
HCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQNHSASLLVELLTEELPPKALARLGDAFAEGIAQRLAARDLIEGELAFERYATPRRLA
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
VVVQNVRAVAPERQVREKVLPVSVALDAQGAPSAPLAKKLAALGHPNLSIADLERAHDGK
HHHHHHHHHCCHHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHCCCC
AEAFFVNYAAPGATLADGLQAALDETLAKLPIPKVMTYQRPDGTDVQFVRPVHRLTVLHG
CCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHEECCCCCCCHHHHHHHHHHHHHCC
DRIVPVAAFGIDAGDTTLGHRFLSDGLVAIRHANAYADTLREKGRVLAHFAQRRETIRTQ
CEEEEEEEECCCCCCHHHHHHHHHCCHHEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
LEANANGDRVVMPEALLDEVTSLVEWPVVYPCRFEDEFLQVPQECLILTMQTNQKYFALT
HHCCCCCCEEECCHHHHHHHHHHHHCCCEECCCCCHHHHHCCHHHEEEEEECCCEEEEEE
DAAGKLRSRFLIVSNIETKTPGEIVEGNERVVRPRLADAKFFFEQDKKKPLAERVPLLAN
CHHHHHHHCEEEEECCCCCCCCCHHCCCCCEECCCHHHHHHHHHHHCCCCHHHHHHHHHH
VVYHNKLGSQLARVERLETLAGEIAPAIGADATVAIRAARLAKADLLTDMVGEFPELQGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHCCC
MGTYYARHDGEPEDVAIACSEHYQPRFSGDALPTTPVSTAVALADKLETIVGIWGIGLAP
HHHEEECCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
TGEKDPFALRRHALGVLRLLLEKQLPLDLVWLLRTAYERFAAVPGVAESTDAIHAFFIDR
CCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHH
LRGLLRERGYTAGEIDAVLSLNPTRLDDIVARLNAVREFTRLAEAEALAAANKRISNILK
HHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KSESGANGAVQPALFVEAAEKALAEQLADVTPRVQSQLEARAYTGALTALAALRAPVDAF
HCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
FDGVMVNADDPALRANRLALLSALHQQMNCVADISKLAA
HCCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA