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The map label for this gene is rfbX [H]

Identifier: 53718246

GI number: 53718246

Start: 679223

End: 680491

Strand: Reverse

Name: rfbX [H]

Synonym: BPSL0603

Alternate gene names: 53718246

Gene position: 680491-679223 (Counterclockwise)

Preceding gene: 53718247

Following gene: 53718238

Centisome position: 16.7

GC content: 67.38

Gene sequence:

>1269_bases
ATGACGACCGTTCGCAAGAATTTCCTGCTGCTGATGACGCTGCAGATCTCCACCTATGTCGTGCCGCTCGTCACGCTGCC
GCTGCTCACGCGCGTGCTCGGGCCCGAAGCGTACGGACAGCTGTCGTTCGTGCTCGCCGTCATCACCTACTTCATCAATT
TCACGAACTACAGCTTCGATCTGACCGCGACGCCGCGCATCGCGCTCGCGCACGACGATCTCGCCGCGCGCTCGAAGATC
TTCTGGGCGACCTTCGCCGCGCAGGCCGCGATCACGGTGGCGGGCTTCGCGATCCTGCTCGCGCTCACGTTCGCGATCGA
GCGGTTCGCGATCGACCGCACGCTGCTCGTGATCGGCTTCGGCATGACGGTGGGCGCCGCGCTCACGCCGGGCTGGTATT
TCCAGGGCATCCAGAAGCTGCGCATCTACAGCATGACGCTGTTCGCGTGCCGCGCGCTCAGCGTGCCCGCGATGTATCTG
TGCGTGCGCTCGCCCGCCGACCTGATCGACGCGGTCGCGATCAACGCGGCCGTGCCGCTCGTGTGCGGCGTGCTCGTGTT
CGGCTACCTGATCGTGCAGCGCGAAGTCGTGTTCGTTCGCATCGGCGCGGCCGACGTCGCCGCGTCGCTCAAGGGCGGCT
GGCAGGTGTTTCTCGCGTCGACGTCAGTCGCGTTCTACGCGTCGACGAACACCGTGCTGCTCGGCTTCGTGTCGGGCAAC
GTCGCGGCGGGCTACTTCGCCGCGGGCGACAAGCTGATCCGCTCGGCCATCAGCCTGCTGCAGCCGCTGAAGGCTGCCGC
GTATCCGCACGTCAGCTATCTGATGCACCACGCGCGCGAGGACGCGATGTCGTTCCTGCGCAAGCTGCTCGTCGTGCAGG
GCGCGATGGTGCTCGCGATCTCGCTCGCGATCTTCGCGCTCGCGCCGCTCGCGGTGCACATCCTGTACGGGTCCACCTAC
GAGCCGACCGTCGGCGTGCTGCGCTGCATGGCGCTCATTCCGTTCATGGCCGGGATGACCGATCTGTTCGGCGTGCAGAC
GATGCTGCCGCTCGGCATGAAGACCGCCTTCAGCCGCGTGCTGATTTCGTCCGGCGTGCTGAATGTCGCGCTGCTGCCGC
CGCTCGCGAAGTGCTTCGCCGAGCAAGGCGCAGCGGCGGCCGTGCTGATCGCCGAGACCGCGGTTGCCGCGACGCTCGCA
TACGTGCTGTATCGGAAGCGCGTATCGATCTTCGCGCGGCCGGCGCCGCGCCCCGCGTCCCGCGGGTGA

Upstream 100 bases:

>100_bases
GAACTCGCGCCACAATGCGATTGACCGATCCGGCTCACCTCGCCGCGCGCGCCATGAGCGCGCGCGGCACGACGTCGCCC
ATGCGACGCGAAGGCCACGC

Downstream 100 bases:

>100_bases
CGGCGGCGAACGGCGGCTGTCGTCGTTCGTCGTCCTCGCCGGTCTCTATCTCCGGCCTTTATCTCCGGTCGCTATTGACG
ATCGCTATTGACGGTCGCTC

Product: putative polysaccharide biosynthesis protein

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 422; Mature: 421

Protein sequence:

>422_residues
MTTVRKNFLLLMTLQISTYVVPLVTLPLLTRVLGPEAYGQLSFVLAVITYFINFTNYSFDLTATPRIALAHDDLAARSKI
FWATFAAQAAITVAGFAILLALTFAIERFAIDRTLLVIGFGMTVGAALTPGWYFQGIQKLRIYSMTLFACRALSVPAMYL
CVRSPADLIDAVAINAAVPLVCGVLVFGYLIVQREVVFVRIGAADVAASLKGGWQVFLASTSVAFYASTNTVLLGFVSGN
VAAGYFAAGDKLIRSAISLLQPLKAAAYPHVSYLMHHAREDAMSFLRKLLVVQGAMVLAISLAIFALAPLAVHILYGSTY
EPTVGVLRCMALIPFMAGMTDLFGVQTMLPLGMKTAFSRVLISSGVLNVALLPPLAKCFAEQGAAAAVLIAETAVAATLA
YVLYRKRVSIFARPAPRPASRG

Sequences:

>Translated_422_residues
MTTVRKNFLLLMTLQISTYVVPLVTLPLLTRVLGPEAYGQLSFVLAVITYFINFTNYSFDLTATPRIALAHDDLAARSKI
FWATFAAQAAITVAGFAILLALTFAIERFAIDRTLLVIGFGMTVGAALTPGWYFQGIQKLRIYSMTLFACRALSVPAMYL
CVRSPADLIDAVAINAAVPLVCGVLVFGYLIVQREVVFVRIGAADVAASLKGGWQVFLASTSVAFYASTNTVLLGFVSGN
VAAGYFAAGDKLIRSAISLLQPLKAAAYPHVSYLMHHAREDAMSFLRKLLVVQGAMVLAISLAIFALAPLAVHILYGSTY
EPTVGVLRCMALIPFMAGMTDLFGVQTMLPLGMKTAFSRVLISSGVLNVALLPPLAKCFAEQGAAAAVLIAETAVAATLA
YVLYRKRVSIFARPAPRPASRG
>Mature_421_residues
TTVRKNFLLLMTLQISTYVVPLVTLPLLTRVLGPEAYGQLSFVLAVITYFINFTNYSFDLTATPRIALAHDDLAARSKIF
WATFAAQAAITVAGFAILLALTFAIERFAIDRTLLVIGFGMTVGAALTPGWYFQGIQKLRIYSMTLFACRALSVPAMYLC
VRSPADLIDAVAINAAVPLVCGVLVFGYLIVQREVVFVRIGAADVAASLKGGWQVFLASTSVAFYASTNTVLLGFVSGNV
AAGYFAAGDKLIRSAISLLQPLKAAAYPHVSYLMHHAREDAMSFLRKLLVVQGAMVLAISLAIFALAPLAVHILYGSTYE
PTVGVLRCMALIPFMAGMTDLFGVQTMLPLGMKTAFSRVLISSGVLNVALLPPLAKCFAEQGAAAAVLIAETAVAATLAY
VLYRKRVSIFARPAPRPASRG

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=396, Percent_Identity=30.3030303030303, Blast_Score=189, Evalue=4e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 45196; Mature: 45065

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTVRKNFLLLMTLQISTYVVPLVTLPLLTRVLGPEAYGQLSFVLAVITYFINFTNYSFD
CCCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEE
LTATPRIALAHDDLAARSKIFWATFAAQAAITVAGFAILLALTFAIERFAIDRTLLVIGF
EECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
GMTVGAALTPGWYFQGIQKLRIYSMTLFACRALSVPAMYLCVRSPADLIDAVAINAAVPL
CHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHH
VCGVLVFGYLIVQREVVFVRIGAADVAASLKGGWQVFLASTSVAFYASTNTVLLGFVSGN
HHHHHHHHHHHHHCEEEEEEECHHHHHHHCCCCEEEEEEECEEEEEEECCEEEEEEECCC
VAAGYFAAGDKLIRSAISLLQPLKAAAYPHVSYLMHHAREDAMSFLRKLLVVQGAMVLAI
CEECHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLAIFALAPLAVHILYGSTYEPTVGVLRCMALIPFMAGMTDLFGVQTMLPLGMKTAFSRV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LISSGVLNVALLPPLAKCFAEQGAAAAVLIAETAVAATLAYVLYRKRVSIFARPAPRPAS
HHHCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC
RG
CC
>Mature Secondary Structure 
TTVRKNFLLLMTLQISTYVVPLVTLPLLTRVLGPEAYGQLSFVLAVITYFINFTNYSFD
CCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCEEE
LTATPRIALAHDDLAARSKIFWATFAAQAAITVAGFAILLALTFAIERFAIDRTLLVIGF
EECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEE
GMTVGAALTPGWYFQGIQKLRIYSMTLFACRALSVPAMYLCVRSPADLIDAVAINAAVPL
CHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHHHHH
VCGVLVFGYLIVQREVVFVRIGAADVAASLKGGWQVFLASTSVAFYASTNTVLLGFVSGN
HHHHHHHHHHHHHCEEEEEEECHHHHHHHCCCCEEEEEEECEEEEEEECCEEEEEEECCC
VAAGYFAAGDKLIRSAISLLQPLKAAAYPHVSYLMHHAREDAMSFLRKLLVVQGAMVLAI
CEECHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLAIFALAPLAVHILYGSTYEPTVGVLRCMALIPFMAGMTDLFGVQTMLPLGMKTAFSRV
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LISSGVLNVALLPPLAKCFAEQGAAAAVLIAETAVAATLAYVLYRKRVSIFARPAPRPAS
HHHCCCHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHEECCCCCCCC
RG
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7517390; 7517391; 9097040; 9278503 [H]