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The map label for this gene is hap [H]

Identifier: 52843170

GI number: 52843170

Start: 3369048

End: 3370397

Strand: Reverse

Name: hap [H]

Synonym: lpg2977

Alternate gene names: 52843170

Gene position: 3370397-3369048 (Counterclockwise)

Preceding gene: 52843171

Following gene: 52843169

Centisome position: 99.19

GC content: 38.67

Gene sequence:

>1350_bases
ATGAATATTACCGGAATTGAACAGGATATTAATTCTACTGAGGGAAAATTGAGCCCGAATGATATAGAACAAAAGACTCT
TGGAAAGGTAACTGAACCGGTTAAATTTAAAAACCTTAAAAAGGTTATTTATATAGATAAAGGAAATAAGGCTCATTTGG
CTTATCATTTGTCTTACTATTCCAATAGTGAAAAAAAACATGTGAATGCACCTAATTATCTTATTGATGCCAACAGCGGT
GAGATTTTGAAACAATGGAATGAGGTGCGTCATGAAAGAATTGGTCAGGGATTAGGCGGTAATGCGTTTACTCTCCCATA
CCGTCAGGGAATGTTTCAGCATGGTAATGCTTTGCCGGGGCTTCCCTCATTAGGGAAGTTTGACGTTAATGTTGAGGACG
GCTTATGTCGTGTTGAAAATGAATCCATAAAAGTAATGAATTTGGAAAATCATAATATAGGTTATGATTTCTTTCCAATT
ACCATATTTGCAGAGTCTGTACTGAATTTAAGTGCCTTTTCCTACCCATGTAATGAAACCAATTTGTTTTTAAACTATGC
TGATGGCAGAACAGGTCCTGTCAATTATGCTTTTTCTCCAGTTAACGATACGATGTATTTTGCCCAACAAACGTTAGATA
TGTATCAAAAAGTTTATGGTGTTAATCGTCCAATAGGTGATGATTTACCTATACGGGCTTACACCCATCTTGGTGACATG
GATAACGCTTTTGCAGTACCAACTATCAGTCTTGATGGGGTAGTTCTTGCACATCAGCAAATTGTAATCGGAAATGGTGA
TGAATTTTTAACAGCTCCCGCCCAGAGTGTATTGGGACATGAATTATCGCACAATTTTACTGCCTTGCATTCCGGATTGA
TGTATGAAGGGCAATCTGGGGGGATCAATGAATCTTTCTCTGATATGGCGGCAATTGCATTGTTAGATTATCTTAGTAAA
GATTATCCATGGTATTGGGATGGTGAGGATTGGACCATTGGGCGTGAAGCTGTAAAAAGTGGGCAACCTATTCGTTATTT
GGATGATCCAGCCAAGGATGGAATGTCTATAGGGCACGCTAGTGAATACACTGATGCGTTAGATGTGCATATAACGAGCG
GAGTATTTAATAAAGCATTTTATTTATTAGCACATAAACCAGGCTGGTCTATACAAAAAGCATTTCAGGTTATGGTTGAC
GCCAATATGAATTATTGGTCTCCTATTGCATACTATGATTTTGCTGCATGTGGCGTCATTCAGGCAACCATAGATAAGCA
TTGGGATAAAACACCTGTTATCGAGGCATTTGCCGAGGTGGGGGTCGTTTGTCCGATGCATAAAAGCTAG

Upstream 100 bases:

>100_bases
AGGCATGCTCGTTATCAAATAACCTACAAGGGGGTTCCGGTTTTGGGGTTATCAAGTCATTTTTCATTCCAAAGATGGCG
CCAAAGAAACTGTAACAGGT

Downstream 100 bases:

>100_bases
TTTTAAGTAGTTTGACTTCAGTTCAAGCTACTTGAAGCAACGAGAGTTGCTAAGGAAGATTGATGATTTTTTATAGAACG
CCTACTGGATAAGATAATTA

Product: zinc metalloprotease (virulence metalloprotease)

Products: NA

Alternate protein names: HA/protease; Vibriolysin [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MNITGIEQDINSTEGKLSPNDIEQKTLGKVTEPVKFKNLKKVIYIDKGNKAHLAYHLSYYSNSEKKHVNAPNYLIDANSG
EILKQWNEVRHERIGQGLGGNAFTLPYRQGMFQHGNALPGLPSLGKFDVNVEDGLCRVENESIKVMNLENHNIGYDFFPI
TIFAESVLNLSAFSYPCNETNLFLNYADGRTGPVNYAFSPVNDTMYFAQQTLDMYQKVYGVNRPIGDDLPIRAYTHLGDM
DNAFAVPTISLDGVVLAHQQIVIGNGDEFLTAPAQSVLGHELSHNFTALHSGLMYEGQSGGINESFSDMAAIALLDYLSK
DYPWYWDGEDWTIGREAVKSGQPIRYLDDPAKDGMSIGHASEYTDALDVHITSGVFNKAFYLLAHKPGWSIQKAFQVMVD
ANMNYWSPIAYYDFAACGVIQATIDKHWDKTPVIEAFAEVGVVCPMHKS

Sequences:

>Translated_449_residues
MNITGIEQDINSTEGKLSPNDIEQKTLGKVTEPVKFKNLKKVIYIDKGNKAHLAYHLSYYSNSEKKHVNAPNYLIDANSG
EILKQWNEVRHERIGQGLGGNAFTLPYRQGMFQHGNALPGLPSLGKFDVNVEDGLCRVENESIKVMNLENHNIGYDFFPI
TIFAESVLNLSAFSYPCNETNLFLNYADGRTGPVNYAFSPVNDTMYFAQQTLDMYQKVYGVNRPIGDDLPIRAYTHLGDM
DNAFAVPTISLDGVVLAHQQIVIGNGDEFLTAPAQSVLGHELSHNFTALHSGLMYEGQSGGINESFSDMAAIALLDYLSK
DYPWYWDGEDWTIGREAVKSGQPIRYLDDPAKDGMSIGHASEYTDALDVHITSGVFNKAFYLLAHKPGWSIQKAFQVMVD
ANMNYWSPIAYYDFAACGVIQATIDKHWDKTPVIEAFAEVGVVCPMHKS
>Mature_449_residues
MNITGIEQDINSTEGKLSPNDIEQKTLGKVTEPVKFKNLKKVIYIDKGNKAHLAYHLSYYSNSEKKHVNAPNYLIDANSG
EILKQWNEVRHERIGQGLGGNAFTLPYRQGMFQHGNALPGLPSLGKFDVNVEDGLCRVENESIKVMNLENHNIGYDFFPI
TIFAESVLNLSAFSYPCNETNLFLNYADGRTGPVNYAFSPVNDTMYFAQQTLDMYQKVYGVNRPIGDDLPIRAYTHLGDM
DNAFAVPTISLDGVVLAHQQIVIGNGDEFLTAPAQSVLGHELSHNFTALHSGLMYEGQSGGINESFSDMAAIALLDYLSK
DYPWYWDGEDWTIGREAVKSGQPIRYLDDPAKDGMSIGHASEYTDALDVHITSGVFNKAFYLLAHKPGWSIQKAFQVMVD
ANMNYWSPIAYYDFAACGVIQATIDKHWDKTPVIEAFAEVGVVCPMHKS

Specific function: May play a role in the pathogenesis of cholera. Hap nicks and activates the A subunit of cholera enterotoxin and related enterotoxins [H]

COG id: COG3227

COG function: function code E; Zinc metalloprotease (elastase)

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M4 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005075
- InterPro:   IPR007280
- InterPro:   IPR013856
- InterPro:   IPR001570
- InterPro:   IPR011096 [H]

Pfam domain/function: PF07504 FTP; PF03413 PepSY; PF01447 Peptidase_M4; PF02868 Peptidase_M4_C; PF04151 PPC [H]

EC number: NA

Molecular weight: Translated: 50010; Mature: 50010

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNITGIEQDINSTEGKLSPNDIEQKTLGKVTEPVKFKNLKKVIYIDKGNKAHLAYHLSYY
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHCCCEEEEEEECCCCEEEEEEEEEC
SNSEKKHVNAPNYLIDANSGEILKQWNEVRHERIGQGLGGNAFTLPYRQGMFQHGNALPG
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHCCCCCCC
LPSLGKFDVNVEDGLCRVENESIKVMNLENHNIGYDFFPITIFAESVLNLSAFSYPCNET
CCCCCEEEEECCCCEEEECCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCC
NLFLNYADGRTGPVNYAFSPVNDTMYFAQQTLDMYQKVYGVNRPIGDDLPIRAYTHLGDM
EEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
DNAFAVPTISLDGVVLAHQQIVIGNGDEFLTAPAQSVLGHELSHNFTALHSGLMYEGQSG
CCCEEEEEEECCCEEEEEEEEEEECCCCEEECCHHHHHHHHHCCCHHHHHCCEEEECCCC
GINESFSDMAAIALLDYLSKDYPWYWDGEDWTIGREAVKSGQPIRYLDDPAKDGMSIGHA
CCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHCCCCEEECCCCCCCCCCCCCC
SEYTDALDVHITSGVFNKAFYLLAHKPGWSIQKAFQVMVDANMNYWSPIAYYDFAACGVI
HHCCCEEEEEEECCHHCCEEEEEEECCCCCHHHHHHHHEECCCCCCCCHHHHHHHHHHHH
QATIDKHWDKTPVIEAFAEVGVVCPMHKS
HHHHHHCCCCCHHHHHHHHCCEECCCCCC
>Mature Secondary Structure
MNITGIEQDINSTEGKLSPNDIEQKTLGKVTEPVKFKNLKKVIYIDKGNKAHLAYHLSYY
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCHHHCCCEEEEEEECCCCEEEEEEEEEC
SNSEKKHVNAPNYLIDANSGEILKQWNEVRHERIGQGLGGNAFTLPYRQGMFQHGNALPG
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEECHHHHHHHHCCCCCCC
LPSLGKFDVNVEDGLCRVENESIKVMNLENHNIGYDFFPITIFAESVLNLSAFSYPCNET
CCCCCEEEEECCCCEEEECCCCEEEEEECCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCC
NLFLNYADGRTGPVNYAFSPVNDTMYFAQQTLDMYQKVYGVNRPIGDDLPIRAYTHLGDM
EEEEEECCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCC
DNAFAVPTISLDGVVLAHQQIVIGNGDEFLTAPAQSVLGHELSHNFTALHSGLMYEGQSG
CCCEEEEEEECCCEEEEEEEEEEECCCCEEECCHHHHHHHHHCCCHHHHHCCEEEECCCC
GINESFSDMAAIALLDYLSKDYPWYWDGEDWTIGREAVKSGQPIRYLDDPAKDGMSIGHA
CCCCCHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHCCCCEEECCCCCCCCCCCCCC
SEYTDALDVHITSGVFNKAFYLLAHKPGWSIQKAFQVMVDANMNYWSPIAYYDFAACGVI
HHCCCEEEEEEECCHHCCEEEEEEECCCCCHHHHHHHHEECCCCCCCCHHHHHHHHHHHH
QATIDKHWDKTPVIEAFAEVGVVCPMHKS
HHHHHHCCCCCHHHHHHHHCCEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2045361; 10952301; 2123831 [H]