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The map label for this gene is wbmI

Identifier: 52843143

GI number: 52843143

Start: 3335790

End: 3337616

Strand: Reverse

Name: wbmI

Synonym: lpg2949

Alternate gene names: 52843143

Gene position: 3337616-3335790 (Counterclockwise)

Preceding gene: 52843144

Following gene: 52843142

Centisome position: 98.23

GC content: 39.03

Gene sequence:

>1827_bases
ATGAATGCTTTGTATGGAGTTCTGCGTTTTGACGGGCAACCATTTGATCGAGAGTTATTAGAACGAGCCAAATTTAATAG
CCCGGTTTGGAGAGCGGATCATAGCGAACTTTATTATAATGATTTTTATGCTACGGCGAGCACACAGCGCTTTATTACTC
CCGAATGCGCCTTACAAACAGCTCCATATCATGATAAGGAATCGGGTGTAATTGCCAACGGCGATCTTTATCTAACTAAT
CATTCCAGTTTGGCAAATTGGTTAGGAGTTAGCTCCAAAAATTCGGATTTATACCTGGCAACCCGTGCATATTTAAAATG
GGGTGAAAAATGTGTGAATTATTTAGCAGGGAATTTTTTCCTTGTATTTTATGACTTACGCAAAAAACGGCTGTTTATTG
CCATTGATCATTTTAATTTTTGTTGCTGTTACTATCTGTTTGTGCCTGGTAAATACTTTATATTTAGCAATACATTCAAT
ACATTCAAAGTTTTTCATGACTCCTTAGCGGTGAATCATGGCGCTTTCCATAAGCTTGCGGCAAAACAGAAAATAGGTCA
GGAAACTTGCCTGAAATCGGTTAAAAAACTGACGGCTGCCAGATATATGATTGTTAGCGACCAAAATTTACAAGAGCAAC
AATACTGGGACCTCCGTAAGACACAACAGACAGGACAATGCCAATCGCGTGAAGACTACTATAGTGCATTCAGAAATGAG
ATTCAGCAAAGTATCAGACAAAGTATCCGGACTGACGCAGAAATCATGGCCCATTACAGCGGTGGTTTAGACTCTTCATC
CATTGCTTCGATTGCTGCCTTGCAACTTGCCAAAGAAAATAAAACTCTTATGGCATATACCGCGATTCCTAAATATTTGT
CGGGCGATAGTCATCGGAAAGGATGTAAATATCATGAATTAGATCGGGTGCAAAGCGCATGCCGTTACTATGACAATATT
GAAACAATAGTTTATCAAAGCTCTGAAGACTTGAGTATTTTTGATAAACTTCAAGTCTTTTATCCTTATTTAGATCAGCC
ATTCAAAGCGGTATTCAATATGGAATGGATATTGGCAGGATTGGAAATGGCAGCAGCCAAAAACTGCAGGATTCTTTTAA
ATGGTGAACAGGGGAATGGATCCATTTCATGGAAGGGAGTGACATTGGGCAACTATCTGAATCAGCGTGTAAAATTTACG
CGGCATCTTTTAAAATACCATATGAAATTAGGCAAGCAATTTATAAAATCTGGCTCGCTAAACCTGAAAAAAAATCGTAC
TGATCTCAATGAGATGAAAAAAAATTTTTTAGAGCCCCATTATTCTCTCCTGAGAGGTAAGAACAGGTTGGAAACAAGAA
GTTACATGCGTTCCATCTATTTGTGGTTTGGGGTAGAGGTGATTGACCCAACAAGTAACCTGGATCTGGTCCAGTTTTGC
TATGATCTTCCTGAATGGGTTTATCATGGAGGTAACAAATATTTTGAAATCAGGCGATTGCCTAAATTAAAAAGGCGCTT
ATTGGTGAGAGATGCTCTGGAAGGGCTTGTGCCCCCTGAGATCCGCCATAATCCCTATCGCGGTGAACAGGCAGCAGATT
GGTACTATCAATTCAATAAATATGGTCATGCCTGGGGAAGGCTTTTGGCAGATATTGCAGAGAAACACCCTCTCATTTGG
GAAATTTATCCTAAAAAATACCTTTTGGATTTATTTGCTAATCATCCCATTAGTCCGCAATCAAGAAATTTTCAAGACGT
TAATATGCATTTGGTCCCAGCTTTAAGCACAGCATTTTTTATCAACTACCTAAATGATCTGTATTGA

Upstream 100 bases:

>100_bases
ACGAATTTATTAGTTTTGCTGTTGCGGTAGTTACATTTTTGGCTGGTTGGAAATTAAGCAGCACAAACATTAGTAGTTAT
TTTTTATCTTAGGATATAGC

Downstream 100 bases:

>100_bases
TTATTACTTACTGGAGATAATCTCGATGAGTAACCATTTACAACAACACAAATATCAATTCGAATATAAAACCAATACAA
TATTAGAACGCTATGACTTG

Product: asparagine synthetase (B)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 608; Mature: 608

Protein sequence:

>608_residues
MNALYGVLRFDGQPFDRELLERAKFNSPVWRADHSELYYNDFYATASTQRFITPECALQTAPYHDKESGVIANGDLYLTN
HSSLANWLGVSSKNSDLYLATRAYLKWGEKCVNYLAGNFFLVFYDLRKKRLFIAIDHFNFCCCYYLFVPGKYFIFSNTFN
TFKVFHDSLAVNHGAFHKLAAKQKIGQETCLKSVKKLTAARYMIVSDQNLQEQQYWDLRKTQQTGQCQSREDYYSAFRNE
IQQSIRQSIRTDAEIMAHYSGGLDSSSIASIAALQLAKENKTLMAYTAIPKYLSGDSHRKGCKYHELDRVQSACRYYDNI
ETIVYQSSEDLSIFDKLQVFYPYLDQPFKAVFNMEWILAGLEMAAAKNCRILLNGEQGNGSISWKGVTLGNYLNQRVKFT
RHLLKYHMKLGKQFIKSGSLNLKKNRTDLNEMKKNFLEPHYSLLRGKNRLETRSYMRSIYLWFGVEVIDPTSNLDLVQFC
YDLPEWVYHGGNKYFEIRRLPKLKRRLLVRDALEGLVPPEIRHNPYRGEQAADWYYQFNKYGHAWGRLLADIAEKHPLIW
EIYPKKYLLDLFANHPISPQSRNFQDVNMHLVPALSTAFFINYLNDLY

Sequences:

>Translated_608_residues
MNALYGVLRFDGQPFDRELLERAKFNSPVWRADHSELYYNDFYATASTQRFITPECALQTAPYHDKESGVIANGDLYLTN
HSSLANWLGVSSKNSDLYLATRAYLKWGEKCVNYLAGNFFLVFYDLRKKRLFIAIDHFNFCCCYYLFVPGKYFIFSNTFN
TFKVFHDSLAVNHGAFHKLAAKQKIGQETCLKSVKKLTAARYMIVSDQNLQEQQYWDLRKTQQTGQCQSREDYYSAFRNE
IQQSIRQSIRTDAEIMAHYSGGLDSSSIASIAALQLAKENKTLMAYTAIPKYLSGDSHRKGCKYHELDRVQSACRYYDNI
ETIVYQSSEDLSIFDKLQVFYPYLDQPFKAVFNMEWILAGLEMAAAKNCRILLNGEQGNGSISWKGVTLGNYLNQRVKFT
RHLLKYHMKLGKQFIKSGSLNLKKNRTDLNEMKKNFLEPHYSLLRGKNRLETRSYMRSIYLWFGVEVIDPTSNLDLVQFC
YDLPEWVYHGGNKYFEIRRLPKLKRRLLVRDALEGLVPPEIRHNPYRGEQAADWYYQFNKYGHAWGRLLADIAEKHPLIW
EIYPKKYLLDLFANHPISPQSRNFQDVNMHLVPALSTAFFINYLNDLY
>Mature_608_residues
MNALYGVLRFDGQPFDRELLERAKFNSPVWRADHSELYYNDFYATASTQRFITPECALQTAPYHDKESGVIANGDLYLTN
HSSLANWLGVSSKNSDLYLATRAYLKWGEKCVNYLAGNFFLVFYDLRKKRLFIAIDHFNFCCCYYLFVPGKYFIFSNTFN
TFKVFHDSLAVNHGAFHKLAAKQKIGQETCLKSVKKLTAARYMIVSDQNLQEQQYWDLRKTQQTGQCQSREDYYSAFRNE
IQQSIRQSIRTDAEIMAHYSGGLDSSSIASIAALQLAKENKTLMAYTAIPKYLSGDSHRKGCKYHELDRVQSACRYYDNI
ETIVYQSSEDLSIFDKLQVFYPYLDQPFKAVFNMEWILAGLEMAAAKNCRILLNGEQGNGSISWKGVTLGNYLNQRVKFT
RHLLKYHMKLGKQFIKSGSLNLKKNRTDLNEMKKNFLEPHYSLLRGKNRLETRSYMRSIYLWFGVEVIDPTSNLDLVQFC
YDLPEWVYHGGNKYFEIRRLPKLKRRLLVRDALEGLVPPEIRHNPYRGEQAADWYYQFNKYGHAWGRLLADIAEKHPLIW
EIYPKKYLLDLFANHPISPQSRNFQDVNMHLVPALSTAFFINYLNDLY

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 70989; Mature: 70989

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALYGVLRFDGQPFDRELLERAKFNSPVWRADHSELYYNDFYATASTQRFITPECALQT
CCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCEEECHHHHHCC
APYHDKESGVIANGDLYLTNHSSLANWLGVSSKNSDLYLATRAYLKWGEKCVNYLAGNFF
CCCCCCCCCEEECCEEEEECCHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCEE
LVFYDLRKKRLFIAIDHFNFCCCYYLFVPGKYFIFSNTFNTFKVFHDSLAVNHGAFHKLA
EEEEEECCCEEEEEEECCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHH
AKQKIGQETCLKSVKKLTAARYMIVSDQNLQEQQYWDLRKTQQTGQCQSREDYYSAFRNE
HHHHCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
IQQSIRQSIRTDAEIMAHYSGGLDSSSIASIAALQLAKENKTLMAYTAIPKYLSGDSHRK
HHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCCCC
GCKYHELDRVQSACRYYDNIETIVYQSSEDLSIFDKLQVFYPYLDQPFKAVFNMEWILAG
CCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LEMAAAKNCRILLNGEQGNGSISWKGVTLGNYLNQRVKFTRHLLKYHMKLGKQFIKSGSL
HHHHHCCCCEEEEECCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NLKKNRTDLNEMKKNFLEPHYSLLRGKNRLETRSYMRSIYLWFGVEVIDPTSNLDLVQFC
CEECCCCCHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHH
YDLPEWVYHGGNKYFEIRRLPKLKRRLLVRDALEGLVPPEIRHNPYRGEQAADWYYQFNK
HHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
YGHAWGRLLADIAEKHPLIWEIYPKKYLLDLFANHPISPQSRNFQDVNMHLVPALSTAFF
HHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHH
INYLNDLY
HHHHHHCC
>Mature Secondary Structure
MNALYGVLRFDGQPFDRELLERAKFNSPVWRADHSELYYNDFYATASTQRFITPECALQT
CCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCCEEECHHHHHCC
APYHDKESGVIANGDLYLTNHSSLANWLGVSSKNSDLYLATRAYLKWGEKCVNYLAGNFF
CCCCCCCCCEEECCEEEEECCHHHHHHHCCCCCCCCEEEEHHHHHHHHHHHHHHHCCCEE
LVFYDLRKKRLFIAIDHFNFCCCYYLFVPGKYFIFSNTFNTFKVFHDSLAVNHGAFHKLA
EEEEEECCCEEEEEEECCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHH
AKQKIGQETCLKSVKKLTAARYMIVSDQNLQEQQYWDLRKTQQTGQCQSREDYYSAFRNE
HHHHCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
IQQSIRQSIRTDAEIMAHYSGGLDSSSIASIAALQLAKENKTLMAYTAIPKYLSGDSHRK
HHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEEECCHHHHCCCCCCC
GCKYHELDRVQSACRYYDNIETIVYQSSEDLSIFDKLQVFYPYLDQPFKAVFNMEWILAG
CCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LEMAAAKNCRILLNGEQGNGSISWKGVTLGNYLNQRVKFTRHLLKYHMKLGKQFIKSGSL
HHHHHCCCCEEEEECCCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NLKKNRTDLNEMKKNFLEPHYSLLRGKNRLETRSYMRSIYLWFGVEVIDPTSNLDLVQFC
CEECCCCCHHHHHHHHHCHHHHHHHCCCHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHH
YDLPEWVYHGGNKYFEIRRLPKLKRRLLVRDALEGLVPPEIRHNPYRGEQAADWYYQFNK
HHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHH
YGHAWGRLLADIAEKHPLIWEIYPKKYLLDLFANHPISPQSRNFQDVNMHLVPALSTAFF
HHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCCCEEEHHHHHHHHH
INYLNDLY
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]