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The map label for this gene is ansA [H]

Identifier: 52843068

GI number: 52843068

Start: 3251055

End: 3252065

Strand: Direct

Name: ansA [H]

Synonym: lpg2873

Alternate gene names: 52843068

Gene position: 3251055-3252065 (Clockwise)

Preceding gene: 52843062

Following gene: 52843070

Centisome position: 95.68

GC content: 41.54

Gene sequence:

>1011_bases
ATGAAAAAACGAGTTTTAATTATCAATACTGGCGGCACAATCAGTAGCATAAAAACATCGCATGGTTATGAGCCTTCACC
GGGATTCATTCAAAAAGCGTTGGCAGACATTCCTGCTCTAAAACATCAAGACATGCCTGACTACGTTATCAAAGAATATC
AGCCGCTACTTGATTCCTCTAATATGACCGTCACGGAATGGAACAGGATTGCCAGAGATATTGCTCACGAATACGAACAG
TTTGACGGATTTGTTGTATTACATGGCACTGATACCATGGCTTACACTGCTTCCGCTTTGTCATTTATGTTGGAGTATTT
GGGAAAACCCGTCATAGTCACTGGTTCACAGATTCCTTTATCCGAGATTCGTAACGATGCGACAGATAATCTAATTACTT
CCTTATGGCTTTGTGCTCATCAACCTATCCATGAAGTATGCATCTACTTTAATCAACATTTGTTTCGAGGCAATCGCACC
CAAAAAATCAGTGCCCAACGCTTCAATGCTTTTGATTCACCCAACTACCCACCCTTGGCATCGATTGGTATCGATATCGA
GTTACATCAACACCGTTTATTGCCACACCCCAAAGCACCGTTCCATCTACAAACCATTACTCCTCACTTTATCGCAAACT
TCCGCCTGTTCCCAGGCTTTGCCTCTGACGTTCTTGAATACATTTTAAATCAACCTTTACGAGGTTTAATCCTTGAAACC
TATGGGGCAGGTAATGCCCAGAACAATGATCCAAGATTTTTAAGTTTGCTTAAGGAGGCTTGCGGCAGGGGGGTTATTAT
TATTAATTGCACCCAATGCCACCAGGGGAAAGTGGAAATGAATCAATACGCAACAGGACATACTTTAAAAGAAGCAGGCT
TGATTAGCGGCCATGATATGACACCTGAGGCGGCTCATTGCAAATTGCTGTATTTATTAAGCAAGAATCTGGAAATCCCT
GAAATCAAAAAACTGATTGAAACCAATTTGTGTGGTGAACTGCAGATTTAA

Upstream 100 bases:

>100_bases
ATTGGGGCTTTTCGCAAGTCTTGAAATTAGTTGTTATTGGTATAGCCAAGTTTTCGCTATACTTATCGAAAGAATAATAA
ATCATTGATTATAAATCCGC

Downstream 100 bases:

>100_bases
GAACGAGGGATCTCCTTGCTCCAACAGAAAGTATCTTTTGGAGACCTCTCGTATGCATTCGAGGCAGAGAAATCAAAATA
CACCCATTGAAAGTAAATTA

Product: L-asparaginase I (cytoplasmic)

Products: NA

Alternate protein names: L-asparaginase I; L-ASNase I; L-asparagine amidohydrolase I [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKKRVLIINTGGTISSIKTSHGYEPSPGFIQKALADIPALKHQDMPDYVIKEYQPLLDSSNMTVTEWNRIARDIAHEYEQ
FDGFVVLHGTDTMAYTASALSFMLEYLGKPVIVTGSQIPLSEIRNDATDNLITSLWLCAHQPIHEVCIYFNQHLFRGNRT
QKISAQRFNAFDSPNYPPLASIGIDIELHQHRLLPHPKAPFHLQTITPHFIANFRLFPGFASDVLEYILNQPLRGLILET
YGAGNAQNNDPRFLSLLKEACGRGVIIINCTQCHQGKVEMNQYATGHTLKEAGLISGHDMTPEAAHCKLLYLLSKNLEIP
EIKKLIETNLCGELQI

Sequences:

>Translated_336_residues
MKKRVLIINTGGTISSIKTSHGYEPSPGFIQKALADIPALKHQDMPDYVIKEYQPLLDSSNMTVTEWNRIARDIAHEYEQ
FDGFVVLHGTDTMAYTASALSFMLEYLGKPVIVTGSQIPLSEIRNDATDNLITSLWLCAHQPIHEVCIYFNQHLFRGNRT
QKISAQRFNAFDSPNYPPLASIGIDIELHQHRLLPHPKAPFHLQTITPHFIANFRLFPGFASDVLEYILNQPLRGLILET
YGAGNAQNNDPRFLSLLKEACGRGVIIINCTQCHQGKVEMNQYATGHTLKEAGLISGHDMTPEAAHCKLLYLLSKNLEIP
EIKKLIETNLCGELQI
>Mature_336_residues
MKKRVLIINTGGTISSIKTSHGYEPSPGFIQKALADIPALKHQDMPDYVIKEYQPLLDSSNMTVTEWNRIARDIAHEYEQ
FDGFVVLHGTDTMAYTASALSFMLEYLGKPVIVTGSQIPLSEIRNDATDNLITSLWLCAHQPIHEVCIYFNQHLFRGNRT
QKISAQRFNAFDSPNYPPLASIGIDIELHQHRLLPHPKAPFHLQTITPHFIANFRLFPGFASDVLEYILNQPLRGLILET
YGAGNAQNNDPRFLSLLKEACGRGVIIINCTQCHQGKVEMNQYATGHTLKEAGLISGHDMTPEAAHCKLLYLLSKNLEIP
EIKKLIETNLCGELQI

Specific function: Unknown

COG id: COG0252

COG function: function code EJ; L-asparaginase/archaeal Glu-tRNAGln amidotransferase subunit D

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the asparaginase 1 family [H]

Homologues:

Organism=Homo sapiens, GI122937331, Length=357, Percent_Identity=39.2156862745098, Blast_Score=237, Evalue=1e-62,
Organism=Escherichia coli, GI1788065, Length=333, Percent_Identity=50.7507507507508, Blast_Score=337, Evalue=6e-94,
Organism=Escherichia coli, GI1789327, Length=288, Percent_Identity=22.2222222222222, Blast_Score=63, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI193207372, Length=361, Percent_Identity=43.4903047091413, Blast_Score=293, Evalue=6e-80,
Organism=Caenorhabditis elegans, GI17558278, Length=361, Percent_Identity=43.4903047091413, Blast_Score=293, Evalue=9e-80,
Organism=Caenorhabditis elegans, GI17558280, Length=361, Percent_Identity=43.4903047091413, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI193207374, Length=361, Percent_Identity=43.4903047091413, Blast_Score=293, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6320527, Length=334, Percent_Identity=23.9520958083832, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24639609, Length=362, Percent_Identity=43.0939226519337, Blast_Score=276, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24645530, Length=357, Percent_Identity=40.0560224089636, Blast_Score=261, Evalue=5e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006033
- InterPro:   IPR006034
- InterPro:   IPR020827 [H]

Pfam domain/function: PF00710 Asparaginase [H]

EC number: =3.5.1.1 [H]

Molecular weight: Translated: 37729; Mature: 37729

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: PS00144 ASN_GLN_ASE_1 ; PS00917 ASN_GLN_ASE_2 ; PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRVLIINTGGTISSIKTSHGYEPSPGFIQKALADIPALKHQDMPDYVIKEYQPLLDSS
CCCEEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
NMTVTEWNRIARDIAHEYEQFDGFVVLHGTDTMAYTASALSFMLEYLGKPVIVTGSQIPL
CCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEEECCCCCH
SEIRNDATDNLITSLWLCAHQPIHEVCIYFNQHLFRGNRTQKISAQRFNAFDSPNYPPLA
HHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCC
SIGIDIELHQHRLLPHPKAPFHLQTITPHFIANFRLFPGFASDVLEYILNQPLRGLILET
CEEEEEEECCCCCCCCCCCCEEEEECCHHHHHCCEECCCHHHHHHHHHHCCCCHHEEEEE
YGAGNAQNNDPRFLSLLKEACGRGVIIINCTQCHQGKVEMNQYATGHTLKEAGLISGHDM
CCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEHHHCCCCCHHHHCCCCCCCCC
TPEAAHCKLLYLLSKNLEIPEIKKLIETNLCGELQI
CCCHHHHEEEEEHHCCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKKRVLIINTGGTISSIKTSHGYEPSPGFIQKALADIPALKHQDMPDYVIKEYQPLLDSS
CCCEEEEEECCCCCEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
NMTVTEWNRIARDIAHEYEQFDGFVVLHGTDTMAYTASALSFMLEYLGKPVIVTGSQIPL
CCCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCEEEECCCCCH
SEIRNDATDNLITSLWLCAHQPIHEVCIYFNQHLFRGNRTQKISAQRFNAFDSPNYPPLA
HHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCCCC
SIGIDIELHQHRLLPHPKAPFHLQTITPHFIANFRLFPGFASDVLEYILNQPLRGLILET
CEEEEEEECCCCCCCCCCCCEEEEECCHHHHHCCEECCCHHHHHHHHHHCCCCHHEEEEE
YGAGNAQNNDPRFLSLLKEACGRGVIIINCTQCHQGKVEMNQYATGHTLKEAGLISGHDM
CCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCEEHHHCCCCCHHHHCCCCCCCCC
TPEAAHCKLLYLLSKNLEIPEIKKLIETNLCGELQI
CCCHHHHEEEEEHHCCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]