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The map label for this gene is rlmE [H]

Identifier: 52842994

GI number: 52842994

Start: 3150972

End: 3151715

Strand: Reverse

Name: rlmE [H]

Synonym: lpg2797

Alternate gene names: 52842994

Gene position: 3151715-3150972 (Counterclockwise)

Preceding gene: 52842995

Following gene: 52842993

Centisome position: 92.76

GC content: 38.58

Gene sequence:

>744_bases
TTGTTTAAAAGACGTATCCACTGCGATATCCCAGATAATAAAATGGTGAATTATTGCACGTTTTGCTGGGAAAAGGTAGT
AAAATTCTGTATCATAGGAAAAATTGTTTTATCTTTATTCGTTATGAATCGTACTAAAAGCAGTAAACGTTGGCTGCAAG
AGCATTTTGATGACGTTTATGTAAAAAAAGCGCAGGCTGAAGGATATCGAAGTCGAGCCGTATATAAACTCAAAGAAATA
GATGACAAAGAGTCACTCATTAAACCAGGCATGACAGTGGTTGATTTAGGGGCTGCTCCTGGTGGCTGGACACAATACGC
CTCAGAAAAGATGAGAGGAAGCGGTCGTCTTGTTGCATTAGATATTTTACCCATGGATGCTTTGCCTAATGTGGAGTTTA
TCCTTGGGGATTTTCGTGAAGATAATGTCTTGCAAGAATTAATTAATTTGATTCCTCAAAGGACTTTAGACTTGTTATTA
TCGGATATGGCCCCAAATATGAGTGGAAGCTCAGCAATAGATATTCCAAGAGCCATGTATCTGGTAGAGCTGGCTTTTGA
TTTCGCGGAAAAAATGTTAAAACCCGGTGGTAATATGCTAGTTAAAATTTTTCATGGCTCGGGTTTTGATGAATTAGTCA
AACAGGCACGAGCGTCTTTTGAAAAAGTGGTAATCCGTAAGCCATCCGCTTCAAGATCTCGTTCAAAAGAAACCTATTTG
CTGGCAAAGGGCTATAATTTATAG

Upstream 100 bases:

>100_bases
CTAACAAGGCTACATTCGTTTCTGCCAATATAGCTTCAGTTAAGCCTTTGGCGCCAAGGAGCACAACGGGCTTTAAATGA
TGAGCCTTGGCTTTAAGTGA

Downstream 100 bases:

>100_bases
TAGTTTTATATATATTTAAAATATGCTTGGCGTTGTTTTGGATGATATGAGCTGTGCAAGAGCTATTTTGGGTCTCGCAA
CAAAATTGTAAGTAACTAAC

Product: ribosomal RNA large subunit methyltransferase J

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MFKRRIHCDIPDNKMVNYCTFCWEKVVKFCIIGKIVLSLFVMNRTKSSKRWLQEHFDDVYVKKAQAEGYRSRAVYKLKEI
DDKESLIKPGMTVVDLGAAPGGWTQYASEKMRGSGRLVALDILPMDALPNVEFILGDFREDNVLQELINLIPQRTLDLLL
SDMAPNMSGSSAIDIPRAMYLVELAFDFAEKMLKPGGNMLVKIFHGSGFDELVKQARASFEKVVIRKPSASRSRSKETYL
LAKGYNL

Sequences:

>Translated_247_residues
MFKRRIHCDIPDNKMVNYCTFCWEKVVKFCIIGKIVLSLFVMNRTKSSKRWLQEHFDDVYVKKAQAEGYRSRAVYKLKEI
DDKESLIKPGMTVVDLGAAPGGWTQYASEKMRGSGRLVALDILPMDALPNVEFILGDFREDNVLQELINLIPQRTLDLLL
SDMAPNMSGSSAIDIPRAMYLVELAFDFAEKMLKPGGNMLVKIFHGSGFDELVKQARASFEKVVIRKPSASRSRSKETYL
LAKGYNL
>Mature_247_residues
MFKRRIHCDIPDNKMVNYCTFCWEKVVKFCIIGKIVLSLFVMNRTKSSKRWLQEHFDDVYVKKAQAEGYRSRAVYKLKEI
DDKESLIKPGMTVVDLGAAPGGWTQYASEKMRGSGRLVALDILPMDALPNVEFILGDFREDNVLQELINLIPQRTLDLLL
SDMAPNMSGSSAIDIPRAMYLVELAFDFAEKMLKPGGNMLVKIFHGSGFDELVKQARASFEKVVIRKPSASRSRSKETYL
LAKGYNL

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit [H]

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family [H]

Homologues:

Organism=Homo sapiens, GI7019377, Length=213, Percent_Identity=34.7417840375587, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI29029591, Length=207, Percent_Identity=35.2657004830918, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI29029589, Length=207, Percent_Identity=35.2657004830918, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI7110661, Length=207, Percent_Identity=35.2657004830918, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI194097365, Length=188, Percent_Identity=30.8510638297872, Blast_Score=101, Evalue=7e-22,
Organism=Escherichia coli, GI1789569, Length=202, Percent_Identity=53.960396039604, Blast_Score=242, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17554650, Length=208, Percent_Identity=31.7307692307692, Blast_Score=110, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17553474, Length=214, Percent_Identity=33.1775700934579, Blast_Score=103, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI71987550, Length=188, Percent_Identity=30.8510638297872, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71987561, Length=188, Percent_Identity=30.8510638297872, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17553860, Length=188, Percent_Identity=30.8510638297872, Blast_Score=95, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=33.1658291457286, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319796, Length=189, Percent_Identity=31.2169312169312, Blast_Score=90, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6321302, Length=133, Percent_Identity=32.3308270676692, Blast_Score=74, Evalue=1e-14,
Organism=Drosophila melanogaster, GI21356387, Length=211, Percent_Identity=35.0710900473934, Blast_Score=128, Evalue=3e-30,
Organism=Drosophila melanogaster, GI18859957, Length=188, Percent_Identity=29.2553191489362, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24647580, Length=216, Percent_Identity=27.3148148148148, Blast_Score=92, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648639, Length=214, Percent_Identity=26.6355140186916, Blast_Score=91, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015507
- InterPro:   IPR002877 [H]

Pfam domain/function: PF01728 FtsJ [H]

EC number: =2.1.1.166 [H]

Molecular weight: Translated: 28069; Mature: 28069

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRRIHCDIPDNKMVNYCTFCWEKVVKFCIIGKIVLSLFVMNRTKSSKRWLQEHFDDVY
CCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VKKAQAEGYRSRAVYKLKEIDDKESLIKPGMTVVDLGAAPGGWTQYASEKMRGSGRLVAL
HHHHHHCCHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEE
DILPMDALPNVEFILGDFREDNVLQELINLIPQRTLDLLLSDMAPNMSGSSAIDIPRAMY
EEECCCCCCCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LVELAFDFAEKMLKPGGNMLVKIFHGSGFDELVKQARASFEKVVIRKPSASRSRSKETYL
HHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEE
LAKGYNL
EECCCCC
>Mature Secondary Structure
MFKRRIHCDIPDNKMVNYCTFCWEKVVKFCIIGKIVLSLFVMNRTKSSKRWLQEHFDDVY
CCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
VKKAQAEGYRSRAVYKLKEIDDKESLIKPGMTVVDLGAAPGGWTQYASEKMRGSGRLVAL
HHHHHHCCHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCCCHHHHHHHHHCCCCCEEEE
DILPMDALPNVEFILGDFREDNVLQELINLIPQRTLDLLLSDMAPNMSGSSAIDIPRAMY
EEECCCCCCCCHHEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
LVELAFDFAEKMLKPGGNMLVKIFHGSGFDELVKQARASFEKVVIRKPSASRSRSKETYL
HHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHEEECCCCCCCCCCCEEE
LAKGYNL
EECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA