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The map label for this gene is truB

Identifier: 52842969

GI number: 52842969

Start: 3120631

End: 3121557

Strand: Reverse

Name: truB

Synonym: lpg2770

Alternate gene names: 52842969

Gene position: 3121557-3120631 (Counterclockwise)

Preceding gene: 52842970

Following gene: 52842968

Centisome position: 91.87

GC content: 39.16

Gene sequence:

>927_bases
CTGATTCAAGTAGTGATGACAACAATTGAATCTCAGTGTTCCATAGATGGAATCCTGCTATTAAATAAACCACAAGGAAT
GACCTCTAACGCTGCTCTTCAGAAAACCAAACATTTGTTTGGTGCTAAAAAAGCTGGTCACACAGGCAGCTTGGATCCTT
TAGCAACCGGCATGCTACCTCTATGCTTCGGAGAAGCGACTAAAATATGCCAGTATCTCCTTAATGCGGATAAAAGCTAT
GAAACAATTGGGAGGCTAGGCATTAAGACAAATACTGCTGATTGTACTGGCGAAGTGATATTTTGCATTGAAAATTATAC
TGTTAGCCATGAGGAAATGATTGCAACCCTAGAAAAATATAAGGGTAAAATCAAGCAAATTCCATCCATGTTTTCTGCTT
TAAAGCACAAGGGGACCCCTTTGTATCGACTTGCCAGAGAAGGTATAGAGATAGAACGTAAGGCCAGGGATATAGTAATC
AGCCAATTGAATTTGGAACAATTTGATGGTGAATGTTTTACATTAACGGTATCATGCAGCAAAGGAACCTATATACGTAA
TTTAGTGGAAGATATTGGTGATACGTTGAAGGCAGGAGCTCATATGACAAAATTGCATAGGCTCTACACTGCAGGGTTTG
AAAATAATCGAATGTACACTCTCGATGAGTTACAGGATATGCCTTTATCACAAAGACTTGCTTGCTTAATCCCTATTGAT
CAGGCCGTTCAACATTTAACACCAGTAATTCTTTCAGATTCTGAGGTAACAGCAATTCGCCAGGGCAAGGTGATTTCAAA
TAAAACTGGTGCTGTTGAAGGAGAAGATTTGCGCCTTTATGGCGAGCAATCCCAATTTATTGGCATTGGCCAAGCTTTAA
TTCATGGTGATATTAAAGCAAAAAGGTTAGTTTCTTTTGCGTTGTAA

Upstream 100 bases:

>100_bases
AGTATTACTCTACGCACTGTGCCGCAACTTCATTTTATTTATGATGAATCGATTGAGTATGGACAGCGATTAAGCCGATT
AATTGATGAAGTAAATCCCC

Downstream 100 bases:

>100_bases
TTACAATTTTTGTAAGAATTGCTGCCTACTTTGTAATAAGTATGAAATTTTTTACACAAATTTATATCCACTACTTGTTT
CATTTGACATGCCTTGGTAG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MIQVVMTTIESQCSIDGILLLNKPQGMTSNAALQKTKHLFGAKKAGHTGSLDPLATGMLPLCFGEATKICQYLLNADKSY
ETIGRLGIKTNTADCTGEVIFCIENYTVSHEEMIATLEKYKGKIKQIPSMFSALKHKGTPLYRLAREGIEIERKARDIVI
SQLNLEQFDGECFTLTVSCSKGTYIRNLVEDIGDTLKAGAHMTKLHRLYTAGFENNRMYTLDELQDMPLSQRLACLIPID
QAVQHLTPVILSDSEVTAIRQGKVISNKTGAVEGEDLRLYGEQSQFIGIGQALIHGDIKAKRLVSFAL

Sequences:

>Translated_308_residues
MIQVVMTTIESQCSIDGILLLNKPQGMTSNAALQKTKHLFGAKKAGHTGSLDPLATGMLPLCFGEATKICQYLLNADKSY
ETIGRLGIKTNTADCTGEVIFCIENYTVSHEEMIATLEKYKGKIKQIPSMFSALKHKGTPLYRLAREGIEIERKARDIVI
SQLNLEQFDGECFTLTVSCSKGTYIRNLVEDIGDTLKAGAHMTKLHRLYTAGFENNRMYTLDELQDMPLSQRLACLIPID
QAVQHLTPVILSDSEVTAIRQGKVISNKTGAVEGEDLRLYGEQSQFIGIGQALIHGDIKAKRLVSFAL
>Mature_308_residues
MIQVVMTTIESQCSIDGILLLNKPQGMTSNAALQKTKHLFGAKKAGHTGSLDPLATGMLPLCFGEATKICQYLLNADKSY
ETIGRLGIKTNTADCTGEVIFCIENYTVSHEEMIATLEKYKGKIKQIPSMFSALKHKGTPLYRLAREGIEIERKARDIVI
SQLNLEQFDGECFTLTVSCSKGTYIRNLVEDIGDTLKAGAHMTKLHRLYTAGFENNRMYTLDELQDMPLSQRLACLIPID
QAVQHLTPVILSDSEVTAIRQGKVISNKTGAVEGEDLRLYGEQSQFIGIGQALIHGDIKAKRLVSFAL

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=259, Percent_Identity=32.046332046332, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI2367200, Length=300, Percent_Identity=43, Blast_Score=249, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6324037, Length=191, Percent_Identity=29.3193717277487, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6323204, Length=257, Percent_Identity=26.0700389105058, Blast_Score=82, Evalue=9e-17,
Organism=Drosophila melanogaster, GI281364189, Length=262, Percent_Identity=24.0458015267176, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364187, Length=262, Percent_Identity=24.0458015267176, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364185, Length=262, Percent_Identity=24.0458015267176, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281364183, Length=262, Percent_Identity=24.0458015267176, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI62471759, Length=262, Percent_Identity=24.0458015267176, Blast_Score=70, Evalue=1e-12,
Organism=Drosophila melanogaster, GI17975520, Length=262, Percent_Identity=24.0458015267176, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240 [H]

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 33972; Mature: 33972

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQVVMTTIESQCSIDGILLLNKPQGMTSNAALQKTKHLFGAKKAGHTGSLDPLATGMLP
CEEEEEEHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHH
LCFGEATKICQYLLNADKSYETIGRLGIKTNTADCTGEVIFCIENYTVSHEEMIATLEKY
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
KGKIKQIPSMFSALKHKGTPLYRLAREGIEIERKARDIVISQLNLEQFDGECFTLTVSCS
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCCCEEEEEEECC
KGTYIRNLVEDIGDTLKAGAHMTKLHRLYTAGFENNRMYTLDELQDMPLSQRLACLIPID
CCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEHHHHHCCCHHHHHEEEEEHH
QAVQHLTPVILSDSEVTAIRQGKVISNKTGAVEGEDLRLYGEQSQFIGIGQALIHGDIKA
HHHHHHCCEEECCCCEEEEECCCEEECCCCCCCCCCEEEECCCCCEEEHHHHHHHCCHHH
KRLVSFAL
HHHHHHCC
>Mature Secondary Structure
MIQVVMTTIESQCSIDGILLLNKPQGMTSNAALQKTKHLFGAKKAGHTGSLDPLATGMLP
CEEEEEEHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHCCHH
LCFGEATKICQYLLNADKSYETIGRLGIKTNTADCTGEVIFCIENYTVSHEEMIATLEKY
HHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHH
KGKIKQIPSMFSALKHKGTPLYRLAREGIEIERKARDIVISQLNLEQFDGECFTLTVSCS
HHHHHHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCCCEEEEEEECC
KGTYIRNLVEDIGDTLKAGAHMTKLHRLYTAGFENNRMYTLDELQDMPLSQRLACLIPID
CCHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEHHHHHCCCHHHHHEEEEEHH
QAVQHLTPVILSDSEVTAIRQGKVISNKTGAVEGEDLRLYGEQSQFIGIGQALIHGDIKA
HHHHHHCCEEECCCCEEEEECCCEEECCCCCCCCCCEEEECCCCCEEEHHHHHHHCCHHH
KRLVSFAL
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA